Entry Database : PDB / ID : 8xi9 Structure visualization Downloads & linksTitle Crystal structure of FRB-FKBP fusion protein in complex with rapamycin ComponentsFRB-FKBP fusion protein Details Keywords SIGNALING PROTEIN / RapamycinFunction / homology Function and homology informationFunction Domain/homology Component
RNA polymerase III type 2 promoter sequence-specific DNA binding / RNA polymerase III type 1 promoter sequence-specific DNA binding / positive regulation of cytoplasmic translational initiation / T-helper 1 cell lineage commitment / positive regulation of pentose-phosphate shunt / regulation of locomotor rhythm / positive regulation of wound healing, spreading of epidermal cells / cellular response to leucine starvation / TFIIIC-class transcription factor complex binding / TORC2 complex ... RNA polymerase III type 2 promoter sequence-specific DNA binding / RNA polymerase III type 1 promoter sequence-specific DNA binding / positive regulation of cytoplasmic translational initiation / T-helper 1 cell lineage commitment / positive regulation of pentose-phosphate shunt / regulation of locomotor rhythm / positive regulation of wound healing, spreading of epidermal cells / cellular response to leucine starvation / TFIIIC-class transcription factor complex binding / TORC2 complex / regulation of membrane permeability / heart valve morphogenesis / macrolide binding / negative regulation of lysosome organization / activin receptor binding / RNA polymerase III type 3 promoter sequence-specific DNA binding / positive regulation of transcription of nucleolar large rRNA by RNA polymerase I / TORC1 complex / cytoplasmic side of membrane / calcineurin-NFAT signaling cascade / nucleus localization / cellular response to methionine / voluntary musculoskeletal movement / regulation of osteoclast differentiation / regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion / TORC1 signaling / signaling receptor inhibitor activity / positive regulation of keratinocyte migration / transforming growth factor beta receptor binding / TGFBR1 LBD Mutants in Cancer / cellular response to L-leucine / MTOR signalling / Amino acids regulate mTORC1 / type I transforming growth factor beta receptor binding / cellular response to nutrient / regulation of autophagosome assembly / Energy dependent regulation of mTOR by LKB1-AMPK / energy reserve metabolic process / negative regulation of activin receptor signaling pathway / heart trabecula formation / negative regulation of cell size / ruffle organization / terminal cisterna / ryanodine receptor complex / cellular response to osmotic stress / I-SMAD binding / negative regulation of protein localization to nucleus / regulation of amyloid precursor protein catabolic process / anoikis / cardiac muscle cell development / protein maturation by protein folding / positive regulation of transcription by RNA polymerase III / ventricular cardiac muscle tissue morphogenesis / negative regulation of calcineurin-NFAT signaling cascade / 'de novo' protein folding / regulation of myelination / negative regulation of macroautophagy / regulation of cell size / positive regulation of oligodendrocyte differentiation / FK506 binding / Macroautophagy / positive regulation of actin filament polymerization / lysosome organization / positive regulation of myotube differentiation / oligodendrocyte differentiation / behavioral response to pain / channel regulator activity / TGF-beta receptor signaling activates SMADs / Constitutive Signaling by AKT1 E17K in Cancer / germ cell development / mTORC1-mediated signalling / CD28 dependent PI3K/Akt signaling / : / protein peptidyl-prolyl isomerization / Calcineurin activates NFAT / HSF1-dependent transactivation / neuronal action potential / regulation of ryanodine-sensitive calcium-release channel activity / regulation of immune response / TOR signaling / response to amino acid / endomembrane system / cellular response to nutrient levels / positive regulation of translational initiation / regulation of macroautophagy / 'de novo' pyrimidine nucleobase biosynthetic process / positive regulation of lamellipodium assembly / positive regulation of epithelial to mesenchymal transition / positive regulation of lipid biosynthetic process / heart morphogenesis / response to nutrient / cardiac muscle contraction / supramolecular fiber organization / regulation of cellular response to heat / phagocytic vesicle / positive regulation of stress fiber assembly / cytoskeleton organization / Regulation of PTEN gene transcription / sarcoplasmic reticulum membrane / T cell costimulation Similarity search - Function Domain of unknown function DUF3385, target of rapamycin protein / Serine/threonine-protein kinase mTOR domain / Domain of unknown function / FKBP12-rapamycin binding domain / Serine/threonine-protein kinase TOR / FKBP12-rapamycin binding domain superfamily / FKBP12-rapamycin binding domain / Rapamycin binding domain / : / FATC domain ... Domain of unknown function DUF3385, target of rapamycin protein / Serine/threonine-protein kinase mTOR domain / Domain of unknown function / FKBP12-rapamycin binding domain / Serine/threonine-protein kinase TOR / FKBP12-rapamycin binding domain superfamily / FKBP12-rapamycin binding domain / Rapamycin binding domain / : / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / : / FKBP-type peptidyl-prolyl cis-trans isomerase domain profile. / FKBP-type peptidyl-prolyl cis-trans isomerase domain / FKBP-type peptidyl-prolyl cis-trans isomerase / Phosphatidylinositol 3- and 4-kinases signature 1. / Phosphatidylinositol 3/4-kinase, conserved site / Phosphatidylinositol 3- and 4-kinases signature 2. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Peptidyl-prolyl cis-trans isomerase domain superfamily / Armadillo-like helical / Tetratricopeptide-like helical domain superfamily / Armadillo-type fold / Protein kinase-like domain superfamily Similarity search - Domain/homology RAPAMYCIN IMMUNOSUPPRESSANT DRUG / Serine/threonine-protein kinase mTOR / Peptidyl-prolyl cis-trans isomerase FKBP1A Similarity search - ComponentBiological species Homo sapiens (human)Method X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution : 1.85 Å DetailsAuthors Inobe, T. / Sakaguchi, R. / Obita, T. / Mukaiyama, A. / Yokoyama, T. / Mizuguchi, M. / Akiyama, S. Funding support Japan, 2items Details Hide detailsOrganization Grant number Country Japan Society for the Promotion of Science (JSPS) JP18H04544 Japan Japan Society for the Promotion of Science (JSPS) JP19K06595 Japan
CitationJournal : Febs Lett. / Year : 2024Title : Structural insights into rapamycin-induced oligomerization of a FRB-FKBP fusion protein.Authors : Inobe, T. / Sakaguchi, R. / Obita, T. / Mukaiyama, A. / Koike, S. / Yokoyama, T. / Mizuguchi, M. / Akiyama, S. History Deposition Dec 19, 2023 Deposition site : PDBJ / Processing site : PDBJRevision 1.0 Aug 7, 2024 Provider : repository / Type : Initial release