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- PDB-8x27: Crystal structure of H5 hemagglutinin from human-infecting H5N8 i... -

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Basic information

Entry
Database: PDB / ID: 8x27
TitleCrystal structure of H5 hemagglutinin from human-infecting H5N8 influenza virus in complex with LSTc
ComponentsHemagglutinin
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM COMPLEX
Function / homology
Function and homology information


viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane
Similarity search - Function
Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin / Haemagglutinin, influenzavirus A/B / Viral capsid/haemagglutinin protein
Similarity search - Domain/homology
6'-sialyl-N-acetyllactosamine / Hemagglutinin
Similarity search - Component
Biological speciesInfluenza A virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.04 Å
AuthorsJin, X.Y. / Han, P. / Song, H. / Qi, J.X.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)82122040 China
CitationJournal: To Be Published
Title: Enhanced binding of Sialyl Lewis X to hemagglutinin contributes to H5N8 mammalian pathogenicity and infectivity
Authors: Jin, X. / Han, P. / Zhang, C. / Maio, A.D. / Shi, Y. / Qi, J. / Bi, Y. / Liu, Y. / Song, H. / Gao, G.F.
History
DepositionNov 9, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0May 14, 2025Provider: repository / Type: Initial release
Revision 1.1May 28, 2025Group: Derived calculations
Category: pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Hemagglutinin
C: Hemagglutinin
D: Hemagglutinin
B: Hemagglutinin
E: Hemagglutinin
F: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)353,06624
Polymers346,1286
Non-polymers6,93818
Water00
1
A: Hemagglutinin
C: Hemagglutinin
D: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)177,40315
Polymers173,0643
Non-polymers4,33912
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area18740 Å2
ΔGint3 kcal/mol
Surface area61800 Å2
MethodPISA
2
B: Hemagglutinin
E: Hemagglutinin
F: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)175,6639
Polymers173,0643
Non-polymers2,5996
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area17500 Å2
ΔGint-17 kcal/mol
Surface area61660 Å2
MethodPISA
Unit cell
Length a, b, c (Å)72.826, 263.929, 131.199
Angle α, β, γ (deg.)90.000, 101.080, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Protein , 1 types, 6 molecules ACDBEF

#1: Protein
Hemagglutinin


Mass: 57687.926 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza A virus / Gene: HA / Production host: Baculovirus transfer vector pFASTBAC1 / References: UniProt: A0A8E4ZAK5

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Sugars , 5 types, 18 molecules

#2: Polysaccharide N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide, Oligosaccharide / Class: Glycan component / Mass: 674.604 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: oligosaccharide / References: 6'-sialyl-N-acetyllactosamine
DescriptorTypeProgram
DNeup5Aca2-6DGalpb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,3,2/[a2122h-1b_1-5_2*NCC/3=O][a2112h-1b_1-5][Aad21122h-2a_2-6_5*NCC/3=O]/1-2-3/a4-b1_b6-c2WURCSPDB2Glycan 1.1.0
[][b-D-GlcpNAc]{[(4+1)][b-D-Galp]{[(6+2)][a-D-Neup5Ac]{}}}LINUCSPDB-CARE
#3: Polysaccharide N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D- ...N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose


Type: oligosaccharide / Mass: 836.744 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DNeup5Aca2-6DGalpb1-4DGlcpNAcb1-3DGalpb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2112h-1b_1-5][a2122h-1b_1-5_2*NCC/3=O][Aad21122h-2a_2-6_5*NCC/3=O]/1-2-1-3/a3-b1_b4-c1_c6-d2WURCSPDB2Glycan 1.1.0
[][b-D-Galp]{[(3+1)][b-D-GlcpNAc]{[(4+1)][b-D-Galp]{[(6+2)][a-D-Neup5Ac]{}}}}LINUCSPDB-CARE
#4: Polysaccharide N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D- ...N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose


Type: oligosaccharide / Mass: 836.744 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DNeup5Aca2-6DGalpb1-3DGlcpNAcb1-3DGalpb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2112h-1b_1-5][a2122h-1b_1-5_2*NCC/3=O][Aad21122h-2a_2-6_5*NCC/3=O]/1-2-1-3/a3-b1_b3-c1_c6-d2WURCSPDB2Glycan 1.1.0
[][b-D-Galp]{[(3+1)][b-D-GlcpNAc]{[(3+1)][b-D-Galp]{[(6+2)][a-D-Neup5Ac]{}}}}LINUCSPDB-CARE
#5: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 1 / Source method: obtained synthetically
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#6: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.57 Å3/Da / Density % sol: 65.59 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop
Details: 3 % dextran sulfate sodium salt, 0.1 M bicine pH 7.7, 18 % w/v PEG20000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL02U1 / Wavelength: 0.979183 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 20, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979183 Å / Relative weight: 1
ReflectionResolution: 3.02→50 Å / Num. obs: 92082 / % possible obs: 96.6 % / Redundancy: 5.3 % / Biso Wilson estimate: 71.12 Å2 / Rmerge(I) obs: 0.149 / Net I/σ(I): 10.896
Reflection shellResolution: 3.02→3.13 Å / Rmerge(I) obs: 1.424 / Num. unique obs: 8926

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
PHENIX1.20.1_4487refinement
HKL-3000data reduction
HKL-3000data scaling
HKL-3000phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.04→31.72 Å / SU ML: 0.3807 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 25.1048
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2418 1987 2.23 %
Rwork0.2112 86983 -
obs0.2119 88970 95.97 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 97.14 Å2
Refinement stepCycle: LAST / Resolution: 3.04→31.72 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms23298 0 459 0 23757
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01124292
X-RAY DIFFRACTIONf_angle_d1.199732907
X-RAY DIFFRACTIONf_chiral_restr0.06653598
X-RAY DIFFRACTIONf_plane_restr0.00884259
X-RAY DIFFRACTIONf_dihedral_angle_d5.72213340
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.04-3.120.34921310.29835682X-RAY DIFFRACTION88.59
3.12-3.20.32751280.28946063X-RAY DIFFRACTION93.35
3.2-3.30.30811450.28666053X-RAY DIFFRACTION93.68
3.3-3.40.3241410.26996006X-RAY DIFFRACTION93.89
3.4-3.520.29111280.25846103X-RAY DIFFRACTION93.85
3.52-3.670.28231410.23876153X-RAY DIFFRACTION95.13
3.67-3.830.25971530.22976212X-RAY DIFFRACTION95.71
3.83-4.030.25381410.20766289X-RAY DIFFRACTION97.01
4.03-4.290.22411410.19416365X-RAY DIFFRACTION98.37
4.29-4.620.19441490.17796374X-RAY DIFFRACTION98.94
4.62-5.080.22431480.17566434X-RAY DIFFRACTION99.34
5.08-5.810.23671500.18326458X-RAY DIFFRACTION99.8
5.81-7.30.20651500.21246525X-RAY DIFFRACTION99.9
7.3-31.720.21410.18746266X-RAY DIFFRACTION95.94
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.04196021948090.463023157671-0.3617977823825.0813811213-3.562737039532.478246686970.2081597502790.4162274060730.553856607303-0.597329745966-0.320037523160.641402457956-1.275815214390.2009662766980.1698391660672.407990240960.3332910896850.1427692072730.8024759770470.3137691092581.053703052512.02953.495-4.22
21.058997001090.6358833087510.008452263030459.89377704302-2.477635257560.7205702673690.08518134211980.711750539922-0.13740904929-1.80916928706-0.199071199024-0.133375645277-0.610940723538-0.2159575206360.1391434785610.8514222464640.03727475669830.05001270649250.9104866679360.07181831458660.37373244359222.39710.237-17.818
31.95900600148-0.0219312580081-0.15993723325.6960616242-0.5721234640972.2738556925-0.04327696146670.518031363587-0.308705083017-1.05412103432-0.0257189477642-0.421931461021-0.1533441807790.2559903067840.07023835748820.56482906625-0.002689640905680.1367600665980.625872094564-0.09378056563250.32340999141429.238-12.512-15.403
41.62079146143-0.269043571146-0.2582640606013.23819800834-0.09748306678522.28093428337-0.05713621432660.198461530563-0.160954740711-0.6796040591810.0233882316825-0.313892734055-0.07616604184080.2770316966480.04302545125760.435585100525-0.04099661296590.09953904421350.533161180462-0.08337741499320.26501857481127.667-12.585-9.531
50.803725358497-0.3764400885730.3612286409188.82073367798-1.25229947820.3655951512490.1908372756160.5144400322220.728433696203-0.6632459405880.04957231597720.0980068833033-1.30404831913-0.152896912721-0.2042554371511.847400930010.1556385266630.260733623790.7424900803140.3279812701980.86331667207317.77146.115-8.41
61.7982179694-0.89885888142-0.4216270927639.51789678867-2.289476014351.291039774180.06324353949320.4338043628170.660136010603-0.214175205370.322686203221-0.359615447054-0.955421715593-0.102417594183-0.3376714275681.17457187099-0.01222003207910.1556988106420.610391759160.1137694351150.54272253445223.12635.6910.755
72.86236546692.16279779851-1.261937249952.51300327654-2.242592689582.486915393760.4934189145230.2569359981681.52656367206-0.802893668958-0.231024203885-0.527366173362-1.843996886990.030951741694-0.2876847721183.3065774023-0.1189795300310.2587864214731.057370269270.312098087161.8585229230422.25281.013-3.217
80.761660625104-0.940896329708-0.4428540440066.77054371054-0.06400910284610.8945025053090.21446253443-0.1112454043280.6997812429040.2988638338590.1978390244810.163071000924-1.3738800929-0.192499083034-0.3910359962921.473582519090.01932291065210.2773662291860.611041022616-0.02440380713370.62241180062312.79341.33725.423
90.0977185319674-1.181568216950.6524598170788.3301738781-1.410500833493.1685408710.181554849949-0.0539735759717-0.3229208904030.430283852423-0.03641591571480.968338333319-0.0944632248457-0.661135069393-0.1330258413050.2019632634380.002409230858210.01131979215880.5510499597050.009831993854950.4505627875827.557-3.67221.481
101.31063311660.25395379047-0.8101440725762.526917062380.2748556313261.90425759179-0.02157894229940.117010497836-0.167542147431-0.0151022850272-0.059104521310.4448857145560.0948162116288-0.4891288076230.0779199733960.169189714172-0.02719902808430.02804134074170.5114815426050.00544528833810.4494656798948.744-16.89515.65
112.26177913212-1.19202828284-0.2995588397818.623101223121.14149083452.12926413630.280173082814-0.1975257516840.7115550329010.246260657332-0.0546657477084-0.0747193115424-1.01193483-0.130090052724-0.2401474128660.8469308447760.04387538577160.2247521636170.554133000328-0.01356251032560.46665800751614.46134.99822.768
122.06411757179-1.227723036970.01023997770614.934754913091.348828036611.43293315632-0.0490258051104-0.1179240160050.617358226767-0.5039692416630.3367993894020.302915604131-1.23234885873-0.13443000173-0.2697838032181.328868456070.120744368760.2687398909530.5966000437220.1167843817260.63201548950815.40440.7216.346
130.106988540448-0.4542869330660.153412167862.09145216144-0.6676069695010.1715990122060.2258224765530.1235737360180.489630064535-1.003941611090.00288140264230.831093993115-0.4403362435-0.0921956418045-0.2582599130142.671913551240.3057664116580.3315436543940.9355475566390.01661889736512.150673237269.95280.04817.805
140.828997508610.329717058539-0.0386052404421.990846915261.523020423531.018982462440.131586578454-0.09972689922650.582054782048-0.301925722795-0.177930917862-1.41388446869-0.6892395372810.5915504434980.0303934774680.73793702191-0.3294069692910.2267829035660.869796883994-0.01193117747710.83223659055146.66227.05614.313
151.756506323870.468521910371-0.4632133177972.472928858630.3095936032011.60701947434-0.0182666462618-0.175182722537-0.335013016311-0.00925127153724-0.000229754094584-0.7145940890.08397318843120.5003177279640.01692172499650.2056957061010.04891194582890.0005829591671510.6862177450220.08794838050520.60224125251643.356-14.84918.764
164.51630968634-4.0944065672.549169749798.46907245458-0.8691153758323.02874645974-0.08126884841290.2297059925890.550487761058-0.0910027580199-0.26571830961-1.17711855289-0.6675807309720.7350096921380.30260836450.591953516761-0.2969977634470.1574496531470.6608416233230.02458015510350.64386305316444.30522.43414.746
171.974531029032.3851511465-0.07597070568699.85295080360.3451810150581.313531208210.165622566412-0.09964521868980.6530546977130.714619990190.136359134633-0.190382832128-1.063629223330.536969457368-0.2217166029921.27423210522-0.2784316555240.1767095875070.677322412971-0.0001200750807640.6867350868535.54244.02612.634
181.356298479750.405577119628-0.009291696702320.539825380860.6308246119921.10379632807-0.00846010654591-0.406344074498-0.2409643747420.344154501675-0.0693517112054-0.4760965067460.0993293161838-0.1353982310870.0618070196472.95076247039-0.3513071954460.3507820037020.9357273620410.03757379957271.5353780833834.55881.10818.082
190.676119025301-0.552419593946-0.09027179254362.61389402181-1.271778554110.82273547254-0.0252675232079-0.378450784888-0.4333773033190.861602259474-0.07289371844021.117227390391.37535238056-0.3206551237020.1316525364451.75102740095-0.3642547402630.4831628074681.03277515390.1056360497491.05092226979-10.493-36.15677.09
202.32342354263-0.650476002551.44365681079.27299483363-2.284275492121.29373310918-0.162004007403-1.038838632560.07372308047581.794680603620.02070699331080.523880999146-0.6495188071610.3737798602110.08854439857571.14231393434-0.2254260308820.08151868439791.081984576410.002356136283940.4326191062569.4693.30487.495
211.049358770930.6607855843010.2910639766732.538198561520.2668306682152.901831494750.278820359625-0.3084229043450.1326985771961.1702026434-0.232909238796-0.208068856827-0.7492488328790.942521707628-0.0603481612371.16470123063-0.3609292412730.05837965280681.206990996750.06420938972830.47863088633118.09411.29386.827
224.985324631853.58686137683-0.7313837973325.60517904253-1.061329771941.196067119690.468582523648-0.502145062856-1.043564666211.16200365891-0.4820744082340.3348957958991.10804148506-0.2576434212730.004931847917441.75122677015-0.3100940851670.2097702436451.041562786160.194940833630.989566475356-11.68-45.31676.685
232.801792754052.59518021123-0.7844664324357.08318767672-3.071729153122.31418617989-0.0146915461275-0.344204947508-0.6149429833540.634556109861-0.104768421377-0.09012461532851.181451937890.05179869601760.163527642881.36713135945-0.08938602425110.2277913578110.8592152956660.1259079736680.5855519067691.06-32.61670.443
240.09205052592450.1350847985120.2642337528010.1117986830390.2381093069260.3872058106970.681599632469-0.375686888807-0.770914885351.45771632587-0.09795026381660.0606033749678-0.3650442308810.0106494690783-0.6967043170422.25685747489-0.3171241356650.28430637751.133737592270.1004455856381.84190284637-16.313-75.21272.551
250.8067185542910.0007399328132590.3866896726686.847202642031.93610787522.836096940270.08260850090830.14863265861-0.267038720093-0.0155060021887-0.1167256429280.1367174797390.948782493217-0.1443002713460.03344280871270.785342631444-0.05085008780790.1337931228660.7220548173370.05090609644230.394868592928-0.9-22.79644.086
261.516530400580.08226958767520.6550893083457.689172421764.036175514763.19385409290.1649703088280.7088442067840.157465590305-0.847676349399-0.06798547378710.136510186192-0.638716154765-0.173115339871-0.08375503042460.537060822260.04932154169350.1578223240490.770096410620.1402271517660.3655499401884.3538.89350.172
270.5420077332860.1344388301410.06242108908533.148031300470.2219021995333.038498881210.202389608835-0.1976881816170.2498265403560.372068639327-0.2020167178870.0509250448484-1.029103909630.3698679328710.004239256618760.845811996494-0.1640985151850.1422478371450.7399376721910.06988796233370.4071341136679.65418.40553.715
284.012765419284.501601458242.471916415159.659431111984.875783214164.009327544860.5982369805620.0688897890349-0.9851196017710.898222310612-0.4748597519610.1401528900041.55705102515-0.619687876562-0.175287740311.4351546653-0.1658580851040.03201047746460.864685363950.03010285881510.861668925057-8.214-44.19444.994
293.155599879871.713516564250.9635876318923.917407622061.429244909712.439419348140.0945790296950.122625196318-0.551865024730.2152261486210.002948724695460.1075024730111.08090778872-0.0360488272889-0.07419742744171.08007360064-0.03906503720140.1034580547950.6878670436020.1118507964620.4939061131731.142-30.43756.196
300.0550547370883-0.482730462797-0.1600562453743.817899677771.195740147190.3082371764920.635445347989-0.5385495053540.3833993923020.528110386458-0.2993771111291.61184322372-0.2210294597530.0982016544976-0.1656494558642.83544256344-0.6203547520720.2523549500191.34724580241-0.0003208536444072.28014389608-20.644-71.25548.61
311.4520487223-0.001702213624530.0886673387840.9591646748651.23490465241.303076153230.2749078017050.240032999281-0.7299764994920.902538343578-0.029369408283-0.3656384219321.24671976060.591402632707-0.244301084571.74023869220.65189714642-0.01532904898791.245676545650.2580332673011.055085381121.902-44.31865.273
323.652771327583.338011413941.056727374367.45265544130.6165797653640.342883942572-0.0469072368377-0.206368175984-0.9461840758960.667491296928-0.11485550394-1.214295840650.1466092836072.3665222130.1284042113970.6645199765030.06788958963850.01285419367022.160359911780.304096761780.87053458041140.058-5.92763.639
330.9317194594760.365122026138-0.01674084781272.61494429482-1.010037227081.882183139750.122855215757-0.01294440756310.08482696537310.304584629478-0.434754696462-0.818433798194-0.11176042871.6295443330.2947256907480.641034870975-0.154027790921-0.005864645760191.97115416710.2916059802070.85155026161739.0722.31561.151
341.32171933462-0.192474157862-0.07365099809526.33823478171.235584465372.046270611980.0374343930907-0.0428120025838-0.807617623212-0.208608011914-0.175925465336-0.0532741740951.513793733340.3912494135170.1593330682171.857420371930.3026690581880.1543933479740.8852087000030.1717168054890.89567368949412.575-45.28862.301
352.559448380671.24090594879-0.2362057539590.654348393155-0.5455456326522.29889801510.3562391568680.583217312143-0.0908346615647-1.58874773371-0.686751337593-0.359609091025-0.628519364272-0.02563634818240.3402607586533.136744246860.4183902399450.1364506752811.306749314010.03494960381921.858476238890.419-80.15555.764
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 1:27 )A1 - 27
2X-RAY DIFFRACTION2( CHAIN A AND RESID 28:72 )A28 - 72
3X-RAY DIFFRACTION3( CHAIN A AND RESID 73:180 )A73 - 180
4X-RAY DIFFRACTION4( CHAIN A AND RESID 181:281 )A181 - 281
5X-RAY DIFFRACTION5( CHAIN A AND RESID 282:366 )A282 - 366
6X-RAY DIFFRACTION6( CHAIN A AND RESID 367:454 )A367 - 454
7X-RAY DIFFRACTION7( CHAIN A AND RESID 455:500 )A455 - 500
8X-RAY DIFFRACTION8( CHAIN C AND RESID 1:46 )C1 - 46
9X-RAY DIFFRACTION9( CHAIN C AND RESID 47:106 )C47 - 106
10X-RAY DIFFRACTION10( CHAIN C AND RESID 107:281 )C107 - 281
11X-RAY DIFFRACTION11( CHAIN C AND RESID 282:345 )C282 - 345
12X-RAY DIFFRACTION12( CHAIN C AND RESID 346:454 )C346 - 454
13X-RAY DIFFRACTION13( CHAIN C AND RESID 455:500 )C455 - 500
14X-RAY DIFFRACTION14( CHAIN D AND RESID 1:72 )D1 - 72
15X-RAY DIFFRACTION15( CHAIN D AND RESID 73:266 )D73 - 266
16X-RAY DIFFRACTION16( CHAIN D AND RESID 267:311 )D267 - 311
17X-RAY DIFFRACTION17( CHAIN D AND RESID 312:454 )D312 - 454
18X-RAY DIFFRACTION18( CHAIN D AND RESID 455:500 )D455 - 500
19X-RAY DIFFRACTION19( CHAIN B AND RESID 1:46 )B1 - 46
20X-RAY DIFFRACTION20( CHAIN B AND RESID 47:106 )B47 - 106
21X-RAY DIFFRACTION21( CHAIN B AND RESID 107:295 )B107 - 295
22X-RAY DIFFRACTION22( CHAIN B AND RESID 296:367 )B296 - 367
23X-RAY DIFFRACTION23( CHAIN B AND RESID 368:455 )B368 - 455
24X-RAY DIFFRACTION24( CHAIN B AND RESID 456:500 )B456 - 500
25X-RAY DIFFRACTION25( CHAIN E AND RESID 1:66 )E1 - 66
26X-RAY DIFFRACTION26( CHAIN E AND RESID 67:115 )E67 - 115
27X-RAY DIFFRACTION27( CHAIN E AND RESID 116:295 )E116 - 295
28X-RAY DIFFRACTION28( CHAIN E AND RESID 296:369 )E296 - 369
29X-RAY DIFFRACTION29( CHAIN E AND RESID 370:454 )E370 - 454
30X-RAY DIFFRACTION30( CHAIN E AND RESID 455:500 )E455 - 500
31X-RAY DIFFRACTION31( CHAIN F AND RESID 1:51 )F1 - 51
32X-RAY DIFFRACTION32( CHAIN F AND RESID 52:106 )F52 - 106
33X-RAY DIFFRACTION33( CHAIN F AND RESID 107:311 )F107 - 311
34X-RAY DIFFRACTION34( CHAIN F AND RESID 312:454 )F312 - 454
35X-RAY DIFFRACTION35( CHAIN F AND RESID 455:500 )F455 - 500

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