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- PDB-8wec: Crystal structure of Arabidopsis thaliana MIK2 ectodomain in comp... -

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Basic information

Entry
Database: PDB / ID: 8wec
TitleCrystal structure of Arabidopsis thaliana MIK2 ectodomain in complex with BAK1 ectodomain and SCOOP12
Components
  • BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
  • MDIS1-interacting receptor like kinase 2
  • SERINE-RICH ENDOGENOUS PEPTIDE (SCOOP)
KeywordsPLANT PROTEIN / LRR receptor-like serine/threonine-protein kinase
Function / homology
Function and homology information


indole glucosinolate biosynthetic process / positive regulation of defense response / pollen tube / pollen tube guidance / response to herbivore / defense response to insect / peptide receptor activity / jasmonic acid biosynthetic process / cellular response to peptide / plasmodesma ...indole glucosinolate biosynthetic process / positive regulation of defense response / pollen tube / pollen tube guidance / response to herbivore / defense response to insect / peptide receptor activity / jasmonic acid biosynthetic process / cellular response to peptide / plasmodesma / receptor serine/threonine kinase binding / peptide binding / response to molecule of bacterial origin / defense response / hepatocyte growth factor receptor activity / macrophage colony-stimulating factor receptor activity / platelet-derived growth factor alpha-receptor activity / platelet-derived growth factor beta-receptor activity / stem cell factor receptor activity / boss receptor activity / protein tyrosine kinase collagen receptor activity / brain-derived neurotrophic factor receptor activity / transmembrane-ephrin receptor activity / GPI-linked ephrin receptor activity / epidermal growth factor receptor activity / fibroblast growth factor receptor activity / insulin-like growth factor receptor activity / receptor protein-tyrosine kinase / eukaryotic translation initiation factor 2alpha kinase activity / 3-phosphoinositide-dependent protein kinase activity / DNA-dependent protein kinase activity / ribosomal protein S6 kinase activity / histone H3S10 kinase activity / histone H2AXS139 kinase activity / histone H3S28 kinase activity / histone H4S1 kinase activity / histone H2BS14 kinase activity / histone H3T3 kinase activity / histone H2AS121 kinase activity / Rho-dependent protein serine/threonine kinase activity / histone H2BS36 kinase activity / histone H3S57 kinase activity / histone H2AT120 kinase activity / AMP-activated protein kinase activity / histone H2AS1 kinase activity / histone H3T6 kinase activity / histone H3T11 kinase activity / histone H3T45 kinase activity / non-specific serine/threonine protein kinase / endosome membrane / signaling receptor binding / protein serine kinase activity / ATP binding / identical protein binding / plasma membrane
Similarity search - Function
: / Leucine-rich repeat-containing N-terminal, plant-type / Leucine rich repeat N-terminal domain / : / Leucine-rich repeat region / Leucine Rich Repeat / Leucine-rich repeat, SDS22-like subfamily / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily ...: / Leucine-rich repeat-containing N-terminal, plant-type / Leucine rich repeat N-terminal domain / : / Leucine-rich repeat region / Leucine Rich Repeat / Leucine-rich repeat, SDS22-like subfamily / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Leucine-rich repeat profile. / Leucine-rich repeat / Leucine-rich repeat domain superfamily / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
beta-D-mannopyranose / MDIS1-interacting receptor like kinase 2 / BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å
AuthorsWan, L.H. / Hu, Y.X. / Wu, H.M.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32000900 China
CitationJournal: Nat.Plants / Year: 2024
Title: Mechanistic study of SCOOPs recognition by MIK2-BAK1 complex reveals the role of N-glycans in plant ligand-receptor-coreceptor complex formation.
Authors: Wu, H. / Wan, L. / Liu, Z. / Jian, Y. / Zhang, C. / Mao, X. / Wang, Z. / Wang, Q. / Hu, Y. / Xiong, L. / Xia, Z. / Xue, J. / Li, S. / He, P. / Shan, L. / Xu, S.
History
DepositionSep 17, 2023Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Nov 6, 2024Provider: repository / Type: Initial release
Revision 1.1Nov 27, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ISSN / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Dec 25, 2024Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: MDIS1-interacting receptor like kinase 2
B: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
C: SERINE-RICH ENDOGENOUS PEPTIDE (SCOOP)
D: MDIS1-interacting receptor like kinase 2
E: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
F: SERINE-RICH ENDOGENOUS PEPTIDE (SCOOP)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)213,94832
Polymers204,0716
Non-polymers9,87726
Water00
1
A: MDIS1-interacting receptor like kinase 2
B: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
C: SERINE-RICH ENDOGENOUS PEPTIDE (SCOOP)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)106,63115
Polymers102,0353
Non-polymers4,59512
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: MDIS1-interacting receptor like kinase 2
E: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
F: SERINE-RICH ENDOGENOUS PEPTIDE (SCOOP)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)107,31717
Polymers102,0353
Non-polymers5,28214
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)73.004, 178.690, 144.161
Angle α, β, γ (deg.)90.00, 90.79, 90.00
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 2 types, 4 molecules ADBE

#1: Protein MDIS1-interacting receptor like kinase 2 / AtMIK2 / Probable LRR receptor-like serine/threonine-protein kinase At4g08850


Mass: 75533.305 Da / Num. of mol.: 2 / Mutation: L137E/D164K/S564F
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: MIK2, At4g08850, T32A17.160 / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: Q8VZG8, non-specific serine/threonine protein kinase
#2: Protein BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 / AtBAK1 / BRI1-associated receptor kinase 1 / Protein ELONGATED / Somatic embryogenesis receptor ...AtBAK1 / BRI1-associated receptor kinase 1 / Protein ELONGATED / Somatic embryogenesis receptor kinase 3 / AtSERK3 / Somatic embryogenesis receptor-like kinase 3


Mass: 25183.758 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: BAK1, ELG, SERK3, At4g33430, F17M5.190 / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: Q94F62, receptor protein-tyrosine kinase, non-specific serine/threonine protein kinase

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Protein/peptide , 1 types, 2 molecules CF

#3: Protein/peptide SERINE-RICH ENDOGENOUS PEPTIDE (SCOOP)


Mass: 1318.399 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Arabidopsis thaliana (thale cress)

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Sugars , 8 types, 26 molecules

#4: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 9 / Source method: obtained synthetically
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-4-deoxy-GlcpNAc]{}}LINUCSPDB-CARE
#5: Polysaccharide alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1- ...alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 716.682 Da / Num. of mol.: 1 / Source method: obtained synthetically
DescriptorTypeProgram
LFucpa1-3[DGlcpNAcb1-4][LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2-1-2/a3-b1_a4-c1_a6-d1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#6: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 2 / Source method: obtained synthetically
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#7: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 2 / Source method: obtained synthetically
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#8: Polysaccharide alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose


Type: oligosaccharide / Mass: 342.297 Da / Num. of mol.: 1 / Source method: obtained synthetically
DescriptorTypeProgram
DManpa1-3DManpb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,2,1/[a1122h-1b_1-5][a1122h-1a_1-5]/1-2/a3-b1WURCSPDB2Glycan 1.1.0
[][b-D-Manp]{[(3+1)][a-D-Manp]{}}LINUCSPDB-CARE
#9: Polysaccharide alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 367.349 Da / Num. of mol.: 2 / Source method: obtained synthetically
DescriptorTypeProgram
LFucpa1-6DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,2,1/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2/a6-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(6+1)][a-L-Fucp]{}}LINUCSPDB-CARE
#10: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C8H15NO6 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#11: Sugar ChemComp-BMA / beta-D-mannopyranose / beta-D-mannose / D-mannose / mannose


Type: D-saccharide, beta linking / Mass: 180.156 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H12O6 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DManpbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
b-D-mannopyranoseCOMMON NAMEGMML 1.0
b-D-ManpIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
ManSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.93 Å3/Da / Density % sol: 75.07 %
Crystal growTemperature: 289 K / Method: evaporation / pH: 5.9 / Details: 0.1 M MES pH 5.9, 22% PEG200, 7% PEG 3000

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Data collection

DiffractionMean temperature: 80 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NFPSS / Beamline: BL19U1 / Wavelength: 0.97853 Å
DetectorType: PSI PILATUS 6M / Detector: PIXEL / Date: Sep 9, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97853 Å / Relative weight: 1
ReflectionResolution: 3.1→50 Å / Num. obs: 66926 / % possible obs: 99.9 % / Redundancy: 6.6 % / CC1/2: 0.99 / Net I/σ(I): 7.5
Reflection shellResolution: 3.1→3.17 Å / Num. unique obs: 6711 / CC1/2: 0.68

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Processing

Software
NameVersionClassification
PHENIX(1.20.1_4487: ???)refinement
XDSdata reduction
Aimlessdata scaling
PHENIX(1.20.1_4487: ???)phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.1→46.15 Å / SU ML: 0.49 / Cross valid method: FREE R-VALUE / σ(F): 1.48 / Phase error: 28.43 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2558 3307 4.95 %
Rwork0.245 --
obs0.2455 66822 99.79 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.1→46.15 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13386 0 0 0 13386
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.005
X-RAY DIFFRACTIONf_angle_d0.794
X-RAY DIFFRACTIONf_dihedral_angle_d18.0565152
X-RAY DIFFRACTIONf_chiral_restr0.0552311
X-RAY DIFFRACTIONf_plane_restr0.0092355
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.1-3.170.38422440.37944262X-RAY DIFFRACTION100
3.17-3.250.35932380.35694165X-RAY DIFFRACTION100
3.25-3.340.35772210.33564249X-RAY DIFFRACTION100
3.34-3.440.32352020.33274238X-RAY DIFFRACTION100
3.44-3.550.30642060.30364207X-RAY DIFFRACTION100
3.55-3.680.29422160.29264246X-RAY DIFFRACTION100
3.68-3.820.28152170.26494230X-RAY DIFFRACTION100
3.82-40.24871970.2414250X-RAY DIFFRACTION100
4-4.210.22511950.22874247X-RAY DIFFRACTION100
4.21-4.470.20932160.20764258X-RAY DIFFRACTION100
4.47-4.810.20032340.20394231X-RAY DIFFRACTION100
4.81-5.30.20242210.2014232X-RAY DIFFRACTION100
5.3-6.060.25732470.23884253X-RAY DIFFRACTION100
6.06-7.630.22422030.21444251X-RAY DIFFRACTION100
7.64-46.150.26252500.22164196X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.53281.99121.66031.8972.08373.61220.0556-0.1996-0.23550.1711-0.0432-0.2220.22520.0893-0.00540.61460.11150.02670.28550.06830.618-47.435131.7193112.4332
22.1588-1.0969-1.98261.57641.83474.85120.29340.7292-0.094-0.3647-0.49930.4479-0.415-1.0960.19890.57520.1865-0.120.6413-0.07180.6219-58.727620.529273.6016
33.6226-3.1396-3.31125.53591.30833.93370.00510.75310.0377-0.7218-0.26050.2429-0.6242-0.72510.24660.59920.0533-0.10910.5059-0.0420.4624-35.83836.961554.1371
46.3622-2.0789-1.2978.0048-1.11535.0762-0.3753-0.1419-0.1540.35340.0301-0.05590.72460.25120.32870.64280.0479-0.02480.38010.00330.3597-26.6068-8.210560.1206
50.9423-1.24520.14343.267-1.66671.5217-0.8178-0.382-0.97941.25860.09640.41331.8182-0.04930.60042.07690.10110.3470.71940.20011.0766-29.4096-27.906469.101
66.29032.374-2.23717.2401-0.5891.99790.3529-1.08450.01711.6942-0.18910.33480.01630.3824-0.15691.27470.16120.11420.86140.00940.6915-37.72766.675984.629
72.791-1.90170.01935.96540.76621.86350.24950.01920.03490.5459-0.26960.27770.44340.4790.0580.95770.017-0.10060.6078-0.13980.5486-36.02266.679476.1058
85.0441-1.23793.63883.72282.68546.3925-0.296-0.2704-0.16421.30310.08530.60771.17060.210.18081.22960.19480.14390.76020.21850.6631-37.8963-2.126682.7069
92.39860.34120.33965.5192.33978.5185-0.5405-0.3617-0.03911.73610.21520.25830.91681.78620.29961.99990.5724-0.20051.13280.09710.8552-32.4576-3.784990.1866
103.77170.44642.88653.5419-0.36772.9488-0.2106-0.7295-0.16081.40040.08240.37091.29360.57120.10711.89130.3910.34021.28410.23390.8038-34.7635-10.977891.2462
113.28880.6142-0.29620.2422-0.72673.3122-0.4661-2.0267-1.25331.85-0.3222-0.55021.130.39660.80582.80930.40950.06291.41550.29321.0052-29.8926-16.841297.9531
124.1769-0.04071.80471.7405-1.84312.6839-0.7436-0.851-0.34470.4445-0.2764-0.53620.35120.6910.98992.76710.8940.13271.83650.55491.2465-24.385-21.668797.6885
132.7604-2.3194-1.69222.18751.91732.0964-0.91330.15750.54831.4302-0.30620.98571.71060.63671.20150.97450.08070.04160.46690.11390.9042-48.739621.19383.5812
142.26950.8472-1.1751.189-0.65891.3273-0.0699-0.2429-0.07790.1012-0.0682-0.16490.01810.21810.12830.40530.0897-0.17550.32010.06240.5301-4.9119-12.762423.8049
152.6822-0.5196-0.60466.0883-1.07353.48850.2141-0.01560.48370.03080.08760.1638-0.7958-0.1789-0.23140.57130.01210.05150.36840.04540.57344.264524.7588-11.6938
169.4886-1.1181.00875.92120.73557.06730.0004-0.0876-1.19670.63650.37641.11230.1077-1.249-0.35330.5103-0.02660.10260.60520.10650.8031-9.71466.28537.7711
178.5695-1.2087-0.65576.26940.38055.3910.19740.0009-0.28160.11710.11030.1443-0.4774-0.9868-0.31070.42880.04420.01120.4880.09310.6113-3.793510.6452.4784
187.3872-0.9160.19016.74480.67183.9639-0.1588-0.39270.7620.85220.31721.5574-0.3437-1.3843-0.12350.67420.28370.12541.0260.26520.9221-14.801215.42728.5227
194.5965-1.5968-0.48869.32712.32924.6776-0.1329-0.28950.64850.80960.49140.9542-0.8755-1.3075-0.33310.97210.38730.22991.0020.1960.8755-16.362923.91519.3695
206.2605-2.4679-1.4927.73853.69722.83550.51920.32760.03860.6754-0.26951.5408-1.0826-2.2338-0.16671.12820.6340.55371.80790.43031.9031-27.324.08597.0234
216.5999-3.429-0.32276.16994.28224.4194-0.0595-1.02450.73371.18030.26291.1031-0.7652-0.8368-0.25451.64640.37180.44180.96750.01741.0929-17.698133.278811.8549
227.8395-3.7029-0.82667.62613.90753.34720.3505-0.0368-0.10610.3157-0.711.934-0.5976-1.46050.28640.87030.33350.27861.31620.18951.4613-27.675833.70186.9141
233.96963.5622-2.85453.6283-1.72143.54470.37190.3757-1.19190.1372-0.1926-0.5617-0.2133-0.1676-0.21080.64580.10690.11280.60320.12271.1919-2.679-8.705115.8693
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 47 through 212 )
2X-RAY DIFFRACTION2chain 'A' and (resid 213 through 442 )
3X-RAY DIFFRACTION3chain 'A' and (resid 443 through 500 )
4X-RAY DIFFRACTION4chain 'A' and (resid 501 through 591 )
5X-RAY DIFFRACTION5chain 'A' and (resid 592 through 683 )
6X-RAY DIFFRACTION6chain 'B' and (resid 26 through 41 )
7X-RAY DIFFRACTION7chain 'B' and (resid 42 through 61 )
8X-RAY DIFFRACTION8chain 'B' and (resid 62 through 95 )
9X-RAY DIFFRACTION9chain 'B' and (resid 96 through 114 )
10X-RAY DIFFRACTION10chain 'B' and (resid 115 through 146 )
11X-RAY DIFFRACTION11chain 'B' and (resid 147 through 185 )
12X-RAY DIFFRACTION12chain 'B' and (resid 186 through 200 )
13X-RAY DIFFRACTION13chain 'C' and (resid 1 through 13 )
14X-RAY DIFFRACTION14chain 'D' and (resid 47 through 442 )
15X-RAY DIFFRACTION15chain 'D' and (resid 443 through 683 )
16X-RAY DIFFRACTION16chain 'E' and (resid 26 through 41 )
17X-RAY DIFFRACTION17chain 'E' and (resid 42 through 79 )
18X-RAY DIFFRACTION18chain 'E' and (resid 80 through 114 )
19X-RAY DIFFRACTION19chain 'E' and (resid 115 through 146 )
20X-RAY DIFFRACTION20chain 'E' and (resid 147 through 157 )
21X-RAY DIFFRACTION21chain 'E' and (resid 158 through 170 )
22X-RAY DIFFRACTION22chain 'E' and (resid 171 through 199 )
23X-RAY DIFFRACTION23chain 'F' and (resid 1 through 13 )

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