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Yorodumi- PDB-8wcp: Cryo-EM structure of human disease-associated P301L Tau amyloid f... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8wcp | ||||||
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Title | Cryo-EM structure of human disease-associated P301L Tau amyloid fibril from mouse brain | ||||||
Components |
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Keywords | PROTEIN FIBRIL / amyloid | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.28 Å | ||||||
Authors | Liu, K.E. / Zhao, W.B. / Liu, C. | ||||||
Funding support | 1items
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Citation | Journal: Cell Discov / Year: 2024 Title: Cryo-EM structures reveal variant Tau amyloid fibrils between the rTg4510 mouse model and sporadic human tauopathies. Authors: Wanbing Zhao / Kaien Liu / Yun Fan / Qinyue Zhao / Youqi Tao / Mengwei Zhang / Linhua Gan / Wenbo Yu / Bo Sun / Dan Li / Cong Liu / Jian Wang / | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8wcp.cif.gz | 58.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8wcp.ent.gz | 35.2 KB | Display | PDB format |
PDBx/mmJSON format | 8wcp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8wcp_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 8wcp_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 8wcp_validation.xml.gz | 26.8 KB | Display | |
Data in CIF | 8wcp_validation.cif.gz | 36.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wc/8wcp ftp://data.pdbj.org/pub/pdb/validation_reports/wc/8wcp | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 40089.898 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) #2: Protein/peptide | Mass: 869.063 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) #3: Protein/peptide | Mass: 783.958 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-Sample preparation
Component | Name: Tau / Type: TISSUE / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Mus musculus (house mouse) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
CTF correction | Type: NONE |
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Helical symmerty | Angular rotation/subunit: -1.36 ° / Axial rise/subunit: 4.78 Å / Axial symmetry: C1 |
3D reconstruction | Resolution: 3.28 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 92188 / Symmetry type: HELICAL |