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- PDB-8wcp: Cryo-EM structure of human disease-associated P301L Tau amyloid f... -

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Basic information

Entry
Database: PDB / ID: 8wcp
TitleCryo-EM structure of human disease-associated P301L Tau amyloid fibril from mouse brain
Components
  • Island A
  • Island B
  • P301L Tau amyloid fibril from rTg4510 mice
KeywordsPROTEIN FIBRIL / amyloid
Biological speciesMus musculus (house mouse)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.28 Å
AuthorsLiu, K.E. / Zhao, W.B. / Liu, C.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Cell Discov / Year: 2024
Title: Cryo-EM structures reveal variant Tau amyloid fibrils between the rTg4510 mouse model and sporadic human tauopathies.
Authors: Wanbing Zhao / Kaien Liu / Yun Fan / Qinyue Zhao / Youqi Tao / Mengwei Zhang / Linhua Gan / Wenbo Yu / Bo Sun / Dan Li / Cong Liu / Jian Wang /
History
DepositionSep 13, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 19, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: P301L Tau amyloid fibril from rTg4510 mice
D: Island B
H: Island A
B: P301L Tau amyloid fibril from rTg4510 mice
E: Island B
G: Island A
C: P301L Tau amyloid fibril from rTg4510 mice
F: Island B
I: Island A


Theoretical massNumber of molelcules
Total (without water)125,2299
Polymers125,2299
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein P301L Tau amyloid fibril from rTg4510 mice


Mass: 40089.898 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
#2: Protein/peptide Island B


Mass: 869.063 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
#3: Protein/peptide Island A


Mass: 783.958 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: helical reconstruction

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Sample preparation

ComponentName: Tau / Type: TISSUE / Entity ID: all / Source: NATURAL
Source (natural)Organism: Mus musculus (house mouse)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 55 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

CTF correctionType: NONE
Helical symmertyAngular rotation/subunit: -1.36 ° / Axial rise/subunit: 4.78 Å / Axial symmetry: C1
3D reconstructionResolution: 3.28 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 92188 / Symmetry type: HELICAL

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