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Yorodumi- EMDB-37445: Cryo-EM structure of human disease-associated P301L Tau amyloid f... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37445 | |||||||||
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Title | Cryo-EM structure of human disease-associated P301L Tau amyloid fibril from mouse brain | |||||||||
Map data | ||||||||||
Sample |
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Keywords | amyloid / PROTEIN FIBRIL | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.28 Å | |||||||||
Authors | Liu KE / Zhao WB / Liu C / Li D | |||||||||
Funding support | 1 items
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Citation | Journal: Cell Discov / Year: 2024 Title: Cryo-EM structures reveal variant Tau amyloid fibrils between the rTg4510 mouse model and sporadic human tauopathies. Authors: Wanbing Zhao / Kaien Liu / Yun Fan / Qinyue Zhao / Youqi Tao / Mengwei Zhang / Linhua Gan / Wenbo Yu / Bo Sun / Dan Li / Cong Liu / Jian Wang / | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37445.map.gz | 30.2 MB | EMDB map data format | |
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Header (meta data) | emd-37445-v30.xml emd-37445.xml | 14.9 KB 14.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_37445_fsc.xml | 12.8 KB | Display | FSC data file |
Images | emd_37445.png | 38.3 KB | ||
Filedesc metadata | emd-37445.cif.gz | 5.1 KB | ||
Others | emd_37445_half_map_1.map.gz emd_37445_half_map_2.map.gz | 140.9 MB 140.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37445 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37445 | HTTPS FTP |
-Validation report
Summary document | emd_37445_validation.pdf.gz | 768.2 KB | Display | EMDB validaton report |
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Full document | emd_37445_full_validation.pdf.gz | 767.7 KB | Display | |
Data in XML | emd_37445_validation.xml.gz | 20 KB | Display | |
Data in CIF | emd_37445_validation.cif.gz | 25.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37445 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37445 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_37445.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_37445_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_37445_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Tau
Entire | Name: Tau |
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Components |
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-Supramolecule #1: Tau
Supramolecule | Name: Tau / type: tissue / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Mus musculus (house mouse) |
-Macromolecule #1: P301L Tau amyloid fibril from rTg4510 mice
Macromolecule | Name: P301L Tau amyloid fibril from rTg4510 mice / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 40.089898 KDa |
Sequence | String: MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV SKSKDGTGSD DKKAKGADG KTKIATPRGA APPGQKGQAN ATRIPAKTPP APKTPPSSGE PPKSGDRSGY SSPGSPGTPG SRSRTPSLPT P PTREPKKV ...String: MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV SKSKDGTGSD DKKAKGADG KTKIATPRGA APPGQKGQAN ATRIPAKTPP APKTPPSSGE PPKSGDRSGY SSPGSPGTPG SRSRTPSLPT P PTREPKKV AVVRTPPKSP SSAKSRLQTA PVPMPDLKNV KSKIGSTENL KHQPGGGKVQ IINKKLDLSN VQSKCGSKDN IK HVLGGGS VQIVYKPVDL SKVTSKCGSL GNIHHKPGGG QVEVKSEKLD FKDRVQSKIG SLDNITHVPG GGNKKIETHK LTF RENAKA KTDHGAEIVY KSPVVSGDTS PRHLSNVSST GSIDMVDSPQ LATLADEVSA SLAKQGL |
-Macromolecule #2: Island B
Macromolecule | Name: Island B / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 869.063 Da |
Sequence | String: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) |
-Macromolecule #3: Island A
Macromolecule | Name: Island A / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 783.958 Da |
Sequence | String: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 55.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |