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- PDB-8vtt: Meis1 homeobox domain bound to neomycin fragment -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 8vtt
TitleMeis1 homeobox domain bound to neomycin fragment
ComponentsHomeobox protein Meis1
KeywordsDNA BINDING PROTEIN / HOMEOBOX DOMAIN / HUMAN HOMEOBOX PROTEIN MEIS1 / DNA-BINDING / TRANSCRIPTION / HOMEOBOX / NUCLEUS / PHOSPHOPROTEIN
Function / homology
Function and homology information


cell growth involved in cardiac muscle cell development / negative regulation of myeloid cell differentiation / lens morphogenesis in camera-type eye / eye development / embryonic pattern specification / definitive hemopoiesis / blood vessel morphogenesis / megakaryocyte development / hemopoiesis / negative regulation of neuron differentiation ...cell growth involved in cardiac muscle cell development / negative regulation of myeloid cell differentiation / lens morphogenesis in camera-type eye / eye development / embryonic pattern specification / definitive hemopoiesis / blood vessel morphogenesis / megakaryocyte development / hemopoiesis / negative regulation of neuron differentiation / locomotory behavior / animal organ morphogenesis / brain development / DNA-binding transcription activator activity, RNA polymerase II-specific / angiogenesis / transcription regulator complex / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin binding / positive regulation of cell population proliferation / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus
Similarity search - Function
Homeobox protein PKNOX/Meis, N-terminal / N-terminal of Homeobox Meis and PKNOX1 / Homeobox KN domain / Homeobox KN domain / 'Homeobox' domain profile. / Homeodomain / Homeobox domain / Homeobox-like domain superfamily
Similarity search - Domain/homology
RIBOSTAMYCIN / Homeobox protein Meis1
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.45 Å
AuthorsTomchick, D.R. / Ahmed, M.S. / Nguyen, N.U.N. / Sadek, H.A.
Funding support United States, France, European Union, United Kingdom, 14items
OrganizationGrant numberCountry
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL137415-02 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL147276-01 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL149137-01 United States
Cancer Prevention and Research Institute of Texas (CPRIT)RP160520 United States
Leducq Foundation France
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM119336 United States
American Heart Association856552 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM127390 United States
Robert A. Welch FoundationI-1505 United States
Leukemia & Lymphoma Society6629-21 United States
European Research Council (ERC)787971European Union
British Heart FoundationRG/19/11/34633 United Kingdom
European Commission825670European Union
European Commission874764European Union
CitationJournal: to be published
Title: Identification of FDA-Approved Drugs That Induce Heart Regeneration in Mammals
Authors: Ahmed, M.S. / Nguyen, N.U.N. / Nakada, Y. / Hsu, C.-C. / Farag, A. / Lam, N.T. / Wang, P. / Thet, S. / Menendez-Montes, I. / Elhelaly, W.M. / Lou, X. / Secco, I. / Tomczyk, M. / Zentilin, L. ...Authors: Ahmed, M.S. / Nguyen, N.U.N. / Nakada, Y. / Hsu, C.-C. / Farag, A. / Lam, N.T. / Wang, P. / Thet, S. / Menendez-Montes, I. / Elhelaly, W.M. / Lou, X. / Secco, I. / Tomczyk, M. / Zentilin, L. / Pei, J. / Cui, M. / Dos Santos, M. / Liu, X. / Liu, Y. / Zaha, D. / Walcott, G. / Tomchick, D.R. / Xing, C. / Zhang, C.-C. / Grishin, N.V. / Giacca, M. / Zhang, J. / Sadek, H.A.
History
DepositionJan 27, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 14, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Homeobox protein Meis1
B: Homeobox protein Meis1
C: Homeobox protein Meis1
D: Homeobox protein Meis1
E: Homeobox protein Meis1
F: Homeobox protein Meis1
G: Homeobox protein Meis1
H: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)62,83810
Polymers62,2878
Non-polymers5512
Water1,09961
1
A: Homeobox protein Meis1


Theoretical massNumber of molelcules
Total (without water)7,7861
Polymers7,7861
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Homeobox protein Meis1


Theoretical massNumber of molelcules
Total (without water)7,7861
Polymers7,7861
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Homeobox protein Meis1


Theoretical massNumber of molelcules
Total (without water)7,7861
Polymers7,7861
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)8,2402
Polymers7,7861
Non-polymers4541
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)7,8822
Polymers7,7861
Non-polymers961
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: Homeobox protein Meis1


Theoretical massNumber of molelcules
Total (without water)7,7861
Polymers7,7861
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: Homeobox protein Meis1


Theoretical massNumber of molelcules
Total (without water)7,7861
Polymers7,7861
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: Homeobox protein Meis1


Theoretical massNumber of molelcules
Total (without water)7,7861
Polymers7,7861
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)77.757, 77.757, 213.298
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number153
Space group name H-MP3212
Space group name HallP322(x,y,z+1/6)
Symmetry operation#1: x,y,z
#2: -y,x-y,z+2/3
#3: -x+y,-x,z+1/3
#4: -y,-x,-z+1/3
#5: -x+y,y,-z+2/3
#6: x,x-y,-z

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Components

#1: Protein
Homeobox protein Meis1 / Myeloid ecotropic viral integration site 1


Mass: 7785.920 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Meis1 / Plasmid: pETDuet / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta / References: UniProt: Q60954
#2: Chemical ChemComp-RIO / RIBOSTAMYCIN / 5-AMINO-2-AMINOMETHYL-6-[4,6-DIAMINO-2-(3,4-DIHYDROXY-5-HYDROXYMETHYL-TETRAHYDRO-FURAN-2-YLOXY)-3-HYDROXY-CYCLOHEXYLOXY ]-TETRAHYDRO-PYRAN-3,4-DIOL / (1R,2R,3S,4R,6S)-4,6-diamino-3-hydroxy-2-(beta-D-ribofuranosyloxy)cyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside


Mass: 454.473 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C17H34N4O10 / Feature type: SUBJECT OF INVESTIGATION / Comment: antibiotic*YM
#3: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 61 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.99 Å3/Da / Density % sol: 58.84 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8
Details: 2.60 M ammonium sulfate, 0.15 M sodium chloride, 20 mM Tris, 4% 2-propanol, 10 mM neomycin, 25% ethylene glycol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97976 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 9, 2021
RadiationMonochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97976 Å / Relative weight: 1
ReflectionResolution: 2.45→50 Å / Num. obs: 27646 / % possible obs: 100 % / Redundancy: 11.9 % / Biso Wilson estimate: 33.85 Å2 / CC1/2: 0.997 / CC star: 0.999 / Rmerge(I) obs: 0.181 / Rpim(I) all: 0.052 / Rrim(I) all: 0.188 / Χ2: 0.972 / Net I/σ(I): 5.2 / Num. measured all: 330178
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2CC starRpim(I) allRrim(I) allΧ2% possible all
2.45-2.496.21.27713600.4790.8050.5561.40.931100
2.49-2.547.21.22613280.5680.8510.4881.3240.952100
2.54-2.5981.13514010.7350.920.4251.2140.958100
2.59-2.6491.12113410.7980.9420.391.1880.97299.9
2.64-2.79.80.98313940.8220.950.3281.0380.973100
2.7-2.7610.10.9213440.8480.9580.30.9690.966100
2.76-2.8311.20.91113740.8870.970.280.9540.974100
2.83-2.9130.83213880.9430.9850.2370.8660.956100
2.9-2.9913.50.7613890.9520.9880.2110.7890.956100
2.99-3.0913.90.63913350.9720.9930.1750.6630.951100
3.09-3.213.90.54413890.9750.9940.1490.5640.954100
3.2-3.3213.50.42513670.9810.9950.1190.4410.983100
3.32-3.4812.70.2913780.9890.9970.0840.3030.989100
3.48-3.6614.50.22813950.9910.9980.0610.2361.002100
3.66-3.8914.20.17713960.9950.9990.0480.1841.02299.7
3.89-4.19140.14113780.9950.9990.0390.1461.022100
4.19-4.6112.90.1113830.9960.9990.0320.1151.056100
4.61-5.2814.50.10314100.9960.9990.0280.1070.953100
5.28-6.6513.20.10414250.9940.9990.030.1080.857100
6.65-5013.20.07314710.9960.9990.0210.0760.96499.6

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
PHENIX1.20.1_4487refinement
HKL-3000data reduction
HKL-3000data scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 2.45→38.88 Å / SU ML: 0.3096 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 27.234
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2569 1992 8.08 %
Rwork0.2149 22657 -
obs0.2184 24649 89.34 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 41.26 Å2
Refinement stepCycle: LAST / Resolution: 2.45→38.88 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3757 0 36 61 3854
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00393897
X-RAY DIFFRACTIONf_angle_d0.42365294
X-RAY DIFFRACTIONf_chiral_restr0.0324581
X-RAY DIFFRACTIONf_plane_restr0.0031669
X-RAY DIFFRACTIONf_dihedral_angle_d14.89671453
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.45-2.510.2839570.2659693X-RAY DIFFRACTION38.5
2.51-2.580.2813760.2508878X-RAY DIFFRACTION49.38
2.58-2.650.33191140.24761249X-RAY DIFFRACTION70.77
2.65-2.740.25981430.24031663X-RAY DIFFRACTION92.19
2.74-2.840.28531610.23951776X-RAY DIFFRACTION98.42
2.84-2.950.29821600.24331777X-RAY DIFFRACTION99.95
2.95-3.080.28991630.23661797X-RAY DIFFRACTION100
3.09-3.250.30371620.24271822X-RAY DIFFRACTION100
3.25-3.450.26891540.22511796X-RAY DIFFRACTION100
3.45-3.720.24121570.20761824X-RAY DIFFRACTION100
3.72-4.090.2481620.20811820X-RAY DIFFRACTION100
4.09-4.680.21841610.17111804X-RAY DIFFRACTION100
4.68-5.890.23551600.20661851X-RAY DIFFRACTION100
5.9-38.880.24021620.20391907X-RAY DIFFRACTION99.52
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.45022582432-0.2747542484890.3821497650619.103340279020.1738374155326.029040380390.3675994697610.342490938680.002167531719580.0812556151622-0.5337563095590.259226459084-0.0928055831328-0.5088697899390.1397294459520.1714280583130.03406926947340.001469349142790.2565504771030.008876626819790.1717253246683.6967977770123.7898318013-39.9619460703
26.337097391380.2794295816931.222147907422.80155909211-2.352433932129.67351328010.29241460438-0.853960414338-0.170593673919-0.149418625542-0.0917245640385-0.3840811217151.24882801760.1035465130150.04542027887960.302979818395-0.01293139264120.05786959237940.296847684131-0.07024507566560.35115069357510.856300231113.6639760564-37.610765218
35.36768292008-0.306645031372.114427264876.1393090718-4.107901320677.93426829270.2587449449990.75950766573-0.468956675234-0.313332236536-0.660191900714-0.02315944254850.8942329784570.6301037987470.2688210266840.281842072315-0.04771850387390.04902932069350.436177844367-0.08993130773010.3013884363089.6014455316721.0911191995-49.6631847428
43.74724349959-0.6098201013160.4937917920256.30233987429-2.854537476444.81864988144-0.4375775382450.08741819317790.4002371419740.7565067722940.137703880171-0.49669743836-0.544563581435-0.1948060951620.1135717202240.20092393279-0.0345052014313-0.04051601577270.261427769680.02373354294360.28231134282714.506321757624.3498882696-35.263034013
55.687800046850.505452990557-1.363917588797.72293069544-0.5505495002276.98142538298-0.387366962996-0.04966756885590.08391443009340.1902128419920.121479937981-0.836273004666-0.7633711693620.8447124366270.3282881003130.3355117307930.0101308017188-0.04788423546290.4556320599870.1106922065540.31469191767211.635364705816.322917575-16.2630414622
64.08433380289-0.497010176147-0.5551115016884.34313767606-1.920511873651.195366825720.01879004989420.991417895808-0.0722876937049-0.828999824586-0.06760670960080.5210181092620.345606154652-0.8457981209920.06216228828020.336110713453-0.0515215564875-0.1063977721420.5502799301330.01776709244240.2389309481360.87423917329112.9400730501-22.927847597
77.167857744640.4425408323994.339693999274.664422277182.016062991367.9951065955-0.262902221076-0.934316505828-0.2328676542570.6628924052610.2167019400950.05194035494030.317341790843-0.5177966953670.05959296311570.3551199500620.0972963100960.0472788661760.2937648145350.05005342102410.2288940354493.6791799815511.4837046501-9.55708521612
80.728126932313-1.400027169632.036787235815.34138583322-2.427314124266.50334308865-0.355976964762-0.129530399610.03769929324140.2510179560310.177379265988-0.316848144965-0.60619134317-0.004621497349950.1725664184020.2227630821860.00979205082292-0.07604393806170.252198939477-0.0258444786130.2213427445313.6131851620523.2661766677-19.2052682382
94.498524077290.8769391187780.571526195094.31184614781-2.177103141294.595670337420.07432900592550.4011040549330.5550229370260.0698751600467-0.1522383197710.0112642192737-0.2272981336950.01487687242350.1170892376130.219639444099-0.005513146904220.04973729298160.4035993991010.09276004949060.48802926490842.003268199236.0409560464-42.2118377178
107.84681950577-1.771813126514.273926965321.925257849810.7975644725144.345981472230.271029479441.17816898071-0.113875345807-0.18183550651-0.04462215679580.09999628847380.1128837266120.570720415153-0.2116313070040.250746114729-0.001992027869860.007432549217470.3739421754690.04644221978420.2943266043243.204803544234.1490280836-48.4006628902
116.203950287321.204331173362.653636256668.474603492113.287958643589.69726313640.223196404810.4604624024210.276763935037-0.07789027737420.07180011851920.301898626959-0.5454202803980.26632431875-0.2134851825960.2761127310030.01758817538890.09527579105820.3222696410890.06517199510560.33428433526120.182198816342.0220248681-44.9726364861
127.83354696364-0.6764713202181.240406825153.40322130173-1.149197980344.988050960630.187079797911-0.407276473133-0.240397058380.361693830590.1568170928050.0346253380661-0.2935957145790.00250478649392-0.2915065271810.340847918283-0.0504155017410.03820813517180.2215241809290.02178509519630.30454457587118.646819940338.9025764769-37.5092946096
133.02495195506-0.923249621573-0.6188171836043.55561332296-0.8011034628164.09392841615-0.05239991899340.187453025776-0.30732398980.170602268205-0.006050064365590.269165089910.0823739900157-0.2417970587690.09455649673840.3353938703370.04991211007220.0372909490810.30530907871-0.06625390198270.21198257733312.6606380406-5.1642184966-9.33646611297
141.70149089664-0.162277634902-1.773555146246.09582982598-4.822018531248.21780833999-0.1159429840520.1864980050090.1769130093960.546215143406-0.0777140238392-0.533394770265-0.521223120103-0.1222419293340.2051023446310.3107393273280.0803196961393-0.01436372185320.257538385595-0.03295985047920.16124838152215.47325516475.45302153826-8.23809265033
154.87264904409-1.98876174302-0.5149300782537.72096061791-3.2612515026.588696576190.06975150996590.308260152855-0.13065726292-0.189118807581-0.210545827643-0.249702230570.2484332694660.07391196295840.05211460055950.2672930049640.06651418686780.001396269385330.260102896741-0.01239381413760.20386344900421.641582661712.7571711879-30.0737864577
165.160197236881.29701903396-1.973066869196.92675531729-1.127004784954.682765290440.42770877708-0.004870128312260.1365981767380.36779459437-0.228586035496-1.13786490662-0.3380989786320.493237192757-0.2135014699050.2522024764340.0647342007152-0.03199768883510.3885490126340.01699226999880.50818903159827.91632797898.11248868778-22.4517662669
178.852044382823.72342860471-0.7710215841786.753002380841.328599855535.216839054680.862985386148-1.26100148529-0.02838222008330.155853480487-0.901332260944-0.3053447774710.16128218654-0.5571582406520.1461800269170.337895159701-0.03729983787230.01476285093710.340212059275-0.1118537361850.39949076421326.9487817463-5.63377480747-19.0644012854
184.75688204723.10394172911-1.653860571017.26035657842-1.483299760416.83637138713-1.020271075940.6993476010870.0460714956182-0.6265016543220.2919213153150.5095403148530.539074645005-1.531453242540.4487054146960.473424941528-0.0751346941691-0.002083277457020.476444025698-0.05395735911870.48344225076720.8273850598-12.2110364599-27.9124405476
198.534454314580.1283679952010.8617123590666.124958808264.245041900927.652163136510.2586279657410.840987827550.19991305664-1.02019866727-0.00189172711208-0.55591996478-1.15783742442-0.025735651992-0.2753446354640.3946444867040.03098647647580.1563216727630.3880962576030.0115290066390.43871331759531.9285025679-2.95293599422-28.9327962866
203.404615572114.62496592258-0.2880756935147.453037207740.2984115707450.9235678608150.584005820631-0.338110446544-0.6317833115510.531161960079-0.456274856431-0.90324579255-0.07725232150260.1627725788020.01394870166550.2915853370140.0571473876149-0.04336227605230.316165331373-0.00910539622780.41292267119129.4425805563-16.3215134945-21.1687316115
216.667163304211.63479835728-0.6786368160576.431533386540.9914785976126.02489288221-0.2762857986530.138265897593-0.02643517602170.1129968487650.029209534576-0.2352253090820.03447403758-0.3614967098730.1888463682080.262784879636-0.0141920908524-0.0132120035360.279637861996-0.04063848602840.1798290785467.22752449956-23.9687549072-14.0888294028
224.274908603235.06835531738-2.536617444979.65223451291-2.611866545594.257515412190.0863537924014-0.680838488638-0.466464404870.54853829803-0.28920112207-1.1139378707-0.04571832622010.2553647217060.1609743717980.388050772746-0.020675336771-0.06193038850040.292828509834-0.05557481462940.27900242797614.8057711141-19.1639058523-7.99063734596
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 280 through 294 )AA280 - 2941 - 15
22chain 'A' and (resid 295 through 301 )AA295 - 30116 - 22
33chain 'A' and (resid 302 through 315 )AA302 - 31523 - 36
44chain 'A' and (resid 316 through 334 )AA316 - 33437 - 55
55chain 'B' and (resid 279 through 293 )BB279 - 2931 - 15
66chain 'B' and (resid 294 through 301 )BB294 - 30116 - 23
77chain 'B' and (resid 302 through 315 )BB302 - 31524 - 37
88chain 'B' and (resid 316 through 334 )BB316 - 33438 - 56
99chain 'C' and (resid 279 through 301 )CC279 - 3011 - 23
1010chain 'C' and (resid 302 through 334 )CC302 - 33424 - 56
1111chain 'D' and (resid 279 through 297 )DD279 - 2971 - 19
1212chain 'D' and (resid 298 through 334 )DD298 - 33420 - 56
1313chain 'E' and (resid 279 through 315 )EF279 - 3151 - 37
1414chain 'E' and (resid 316 through 334 )EF316 - 33438 - 56
1515chain 'F' and (resid 279 through 315 )FH279 - 3151 - 37
1616chain 'F' and (resid 316 through 334 )FH316 - 33438 - 56
1717chain 'G' and (resid 279 through 294 )GI279 - 2941 - 16
1818chain 'G' and (resid 295 through 301 )GI295 - 30117 - 23
1919chain 'G' and (resid 302 through 315 )GI302 - 31524 - 37
2020chain 'G' and (resid 316 through 334 )GI316 - 33438 - 56
2121chain 'H' and (resid 279 through 315 )HJ279 - 3151 - 37
2222chain 'H' and (resid 316 through 334 )HJ316 - 33438 - 56

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