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- PDB-8vts: Meis1 homeobox domain bound to paromomycin fragment -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 8vts
TitleMeis1 homeobox domain bound to paromomycin fragment
ComponentsHomeobox protein Meis1
KeywordsDNA BINDING PROTEIN / HOMEOBOX DOMAIN / HUMAN HOMEOBOX PROTEIN MEIS2 / DNA-BINDING / TRANSCRIPTION / HOMEOBOX / NUCLEUS / PHOSPHOPROTEIN
Function / homology
Function and homology information


cell growth involved in cardiac muscle cell development / negative regulation of myeloid cell differentiation / lens morphogenesis in camera-type eye / eye development / embryonic pattern specification / definitive hemopoiesis / blood vessel morphogenesis / megakaryocyte development / hemopoiesis / negative regulation of neuron differentiation ...cell growth involved in cardiac muscle cell development / negative regulation of myeloid cell differentiation / lens morphogenesis in camera-type eye / eye development / embryonic pattern specification / definitive hemopoiesis / blood vessel morphogenesis / megakaryocyte development / hemopoiesis / negative regulation of neuron differentiation / locomotory behavior / animal organ morphogenesis / brain development / DNA-binding transcription activator activity, RNA polymerase II-specific / angiogenesis / transcription regulator complex / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin binding / positive regulation of cell population proliferation / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus
Similarity search - Function
Homeobox protein PKNOX/Meis, N-terminal / N-terminal of Homeobox Meis and PKNOX1 / Homeobox KN domain / Homeobox KN domain / 'Homeobox' domain profile. / Homeodomain / Homeobox domain / Homeobox-like domain superfamily
Similarity search - Domain/homology
ISOPROPYL ALCOHOL / RIBOSTAMYCIN / Homeobox protein Meis1
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 1.91 Å
AuthorsTomchick, D.R. / Ahmed, M.S. / Nguyen, N.U.N.
Funding support United States, France, European Union, United Kingdom, 14items
OrganizationGrant numberCountry
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL137415-02 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL147276-01 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL149137-01 United States
Cancer Prevention and Research Institute of Texas (CPRIT)RP160520 United States
Leducq Foundation France
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM119336 United States
American Heart Association856552 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM127390 United States
Robert A. Welch FoundationI-1505 United States
Leukemia & Lymphoma Society6629-21 United States
European Research Council (ERC)787971European Union
British Heart FoundationRG/19/11/34633 United Kingdom
European Commission825670European Union
European Commission874764European Union
CitationJournal: to be published
Title: Identification of FDA-Approved Drugs That Induce Heart Regeneration in Mammals
Authors: Ahmed, M.S. / Nguyen, N.U.N. / Nakada, Y. / Hsu, C.-C. / Farag, A. / Lam, N.T. / Wang, P. / Thet, S. / Menendez-Montes, I. / Elhelaly, W.M. / Lou, X. / Secco, I. / Tomczyk, M. / Zentilin, L. ...Authors: Ahmed, M.S. / Nguyen, N.U.N. / Nakada, Y. / Hsu, C.-C. / Farag, A. / Lam, N.T. / Wang, P. / Thet, S. / Menendez-Montes, I. / Elhelaly, W.M. / Lou, X. / Secco, I. / Tomczyk, M. / Zentilin, L. / Pei, J. / Cui, M. / Dos Santos, M. / Liu, X. / Liu, Y. / Zaha, D. / Walcott, G. / Tomchick, D.R. / Xing, C. / Zhang, C.-C. / Grishin, N.V. / Giacca, M. / Zhang, J. / Sadek, H.A.
History
DepositionJan 27, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 14, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Homeobox protein Meis1
B: Homeobox protein Meis1
C: Homeobox protein Meis1
D: Homeobox protein Meis1
E: Homeobox protein Meis1
F: Homeobox protein Meis1
G: Homeobox protein Meis1
H: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)63,65917
Polymers62,2878
Non-polymers1,3719
Water7,008389
1
A: Homeobox protein Meis1


Theoretical massNumber of molelcules
Total (without water)7,7861
Polymers7,7861
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)8,3023
Polymers7,7861
Non-polymers5172
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)7,8462
Polymers7,7861
Non-polymers601
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)8,3023
Polymers7,7861
Non-polymers5172
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)7,8462
Polymers7,7861
Non-polymers601
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)7,8822
Polymers7,7861
Non-polymers961
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)7,8462
Polymers7,7861
Non-polymers601
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: Homeobox protein Meis1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)7,8482
Polymers7,7861
Non-polymers621
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)77.621, 77.621, 213.724
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number153
Space group name H-MP3212
Space group name HallP322(x,y,z+1/6)
Symmetry operation#1: x,y,z
#2: -y,x-y,z+2/3
#3: -x+y,-x,z+1/3
#4: -y,-x,-z+1/3
#5: -x+y,y,-z+2/3
#6: x,x-y,-z
Components on special symmetry positions
IDModelComponents
11A-444-

HOH

21A-447-

HOH

31A-449-

HOH

41E-532-

HOH

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Components

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Protein , 1 types, 8 molecules ABCDEFGH

#1: Protein
Homeobox protein Meis1 / Myeloid ecotropic viral integration site 1


Mass: 7785.920 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Meis1 / Plasmid: pETDuet / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta / References: UniProt: Q60954

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Non-polymers , 5 types, 398 molecules

#2: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Formula: C2H6O2
#3: Chemical ChemComp-RIO / RIBOSTAMYCIN / 5-AMINO-2-AMINOMETHYL-6-[4,6-DIAMINO-2-(3,4-DIHYDROXY-5-HYDROXYMETHYL-TETRAHYDRO-FURAN-2-YLOXY)-3-HYDROXY-CYCLOHEXYLOXY ]-TETRAHYDRO-PYRAN-3,4-DIOL / (1R,2R,3S,4R,6S)-4,6-diamino-3-hydroxy-2-(beta-D-ribofuranosyloxy)cyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside


Mass: 454.473 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C17H34N4O10 / Feature type: SUBJECT OF INVESTIGATION / Comment: antibiotic*YM
#4: Chemical ChemComp-IPA / ISOPROPYL ALCOHOL / 2-PROPANOL


Mass: 60.095 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H8O / Comment: alkaloid*YM
#5: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 389 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.99 Å3/Da / Density % sol: 58.84 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8
Details: 2.20 M ammonium sulfate, 0.15 M sodium chloride, 20 mM Tris, 4% 2-propanol, 10 mM paromomycin, 25% ethylene glycol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97976 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 8, 2021
RadiationMonochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97976 Å / Relative weight: 1
ReflectionResolution: 1.9→50 Å / Num. obs: 57573 / % possible obs: 100 % / Redundancy: 12.5 % / Biso Wilson estimate: 20.86 Å2 / CC1/2: 0.997 / CC star: 0.999 / Rmerge(I) obs: 0.079 / Rpim(I) all: 0.023 / Rrim(I) all: 0.082 / Χ2: 0.984 / Net I/σ(I): 6.8 / Num. measured all: 717867
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2CC starRpim(I) allRrim(I) allΧ2% possible all
1.9-1.9310.71.80828970.5320.8330.5731.8990.931100
1.93-1.9711.41.56827970.5780.8560.4831.6430.92100
1.97-2.0111.41.19228330.7060.910.3681.2490.93100
2.01-2.0510.60.97728840.7960.9410.3131.0270.94100
2.05-2.0911.80.79628750.8720.9650.2410.8330.966100
2.09-2.1412.30.60728430.9140.9770.180.6340.97100
2.14-2.1912.30.48128440.9430.9850.1430.5021.008100
2.19-2.2512.30.39828700.9650.9910.1180.4161.007100
2.25-2.3212.20.31828420.9730.9930.0950.3331.002100
2.32-2.3912.10.25528470.9840.9960.0770.2671.039100
2.39-2.4811.30.21728850.9850.9960.0670.2271.036100
2.48-2.5812.80.17528850.9920.9980.0510.1821.041100
2.58-2.713.40.14128430.9950.9990.040.1461.031100
2.7-2.8413.50.11228830.9960.9990.0320.1171.03100
2.84-3.0213.50.08928880.9970.9990.0250.0921.004100
3.02-3.2512.60.07128870.9980.9990.0210.0741.028100
3.25-3.5814.40.05328920.99910.0140.0551.005100
3.58-4.0914.30.04529060.99910.0120.0461.003100
4.09-5.1613.10.0429470.99910.0110.0410.94100
5.16-5013.40.03730250.99910.010.0380.83999.9

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
PHENIX1.20.1_4487refinement
HKL-3000data scaling
HKL-3000data reduction
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 1.91→41.83 Å / SU ML: 0.2177 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 20.7794
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2109 2000 3.57 %
Rwork0.1909 54065 -
obs0.1916 56065 96.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 28.47 Å2
Refinement stepCycle: LAST / Resolution: 1.91→41.83 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3760 0 91 389 4240
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00364001
X-RAY DIFFRACTIONf_angle_d0.48885441
X-RAY DIFFRACTIONf_chiral_restr0.0355603
X-RAY DIFFRACTIONf_plane_restr0.0037683
X-RAY DIFFRACTIONf_dihedral_angle_d14.52651491
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.91-1.960.297930.2822533X-RAY DIFFRACTION63.68
1.96-2.010.31361400.26183734X-RAY DIFFRACTION95.35
2.01-2.070.2861420.22963913X-RAY DIFFRACTION98.85
2.07-2.130.21811530.20693944X-RAY DIFFRACTION99.93
2.13-2.210.21421550.18433962X-RAY DIFFRACTION99.98
2.21-2.30.19911410.18063950X-RAY DIFFRACTION100
2.3-2.40.20911430.16733942X-RAY DIFFRACTION100
2.4-2.530.17411460.17213958X-RAY DIFFRACTION99.98
2.53-2.690.20211440.17263992X-RAY DIFFRACTION100
2.69-2.90.19641510.17943968X-RAY DIFFRACTION100
2.9-3.190.22341450.18993978X-RAY DIFFRACTION100
3.19-3.650.20971540.18084004X-RAY DIFFRACTION100
3.65-4.60.18491460.16954041X-RAY DIFFRACTION100
4.6-41.830.21241470.21924146X-RAY DIFFRACTION99.84
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.60039888248-0.185865574908-0.02860428918895.45861340036-1.074482266734.781708308760.1525923572410.03270687783410.284574033353-0.438352871256-0.00816198802736-0.4253500889030.06855403423690.120220840039-0.1637037172170.1778810087580.06376700725460.04792980170770.148410333807-0.01085274411670.28594592743154.0871183165-21.2869531906-8.13253072865
25.03386476878-4.034589665892.733926063038.83326244815-1.683770771981.645789163910.249875122949-0.1089729613210.103289635563-0.143362029569-0.02826345970640.2432764456750.304387759571-0.277212402228-0.1800191945050.2802761652810.04503634341570.03920290754540.1719355999760.0335053263440.19291229509746.8765881891-16.1181628546-14.2595699708
32.52737796337-0.7718835582832.380088113196.384467075920.2640773009532.5040905847-0.01845278544360.4670398467290.0863778980049-0.449061310420.0886351851628-0.0522840165155-0.04249240432090.342509449197-0.02229226529150.199678613254-0.01376066029820.1031865324510.195704628877-0.01124681146810.23041487989444.26702564694.90440690502-11.554366605
43.29324958951.56934900394-1.512214938972.94407457994-1.191087199689.32858563651-0.0317934885676-0.223099501183-0.22434673879-0.0398310658672-0.177527025646-1.050511023910.4581573028851.03537923910.1163146212870.1552669063150.03409149349840.02404819548340.302847250156-0.02821996578620.37987009634651.53752126970.0379191611482-0.809538423844
57.16799528501-4.590208741182.739495277236.77702711388-4.666571987027.12068505265-0.0319070014760.2325072114930.299033822860.6117469007780.0694789543839-0.0630335211706-0.6700999992350.6372686934270.03584842820110.224312645221-0.07958505273540.09294430934340.17477959726-0.04378328737820.27536536091745.04178440312.5998194739-2.44232824367
63.13188704799-4.469891158230.4070124860436.54471963702-0.8610886919923.610242401720.152245709253-0.213878022929-0.263975492393-0.2142845685230.2617332083780.5736236659220.215511234067-0.03220124970610.01897422776210.101604439553-0.003443036735190.06183244802310.1620792475110.03320146532080.27898172427440.2737723064-1.49927049181-2.03248615821
74.159200725110.882377581377-1.320371725268.003665327840.3887885548417.62506237881-0.173483611984-0.138813239715-0.115855847724-0.0206673439380.1126007623950.0635979111017-0.258349296982-0.05186956491960.05963349186030.1252740123180.0217029533304-0.05609048177390.180968683327-0.0388764320220.177603103036-17.1475651266-18.167031859121.4653774148
84.65358307598-3.2732861163-4.352314963913.254831156712.494011839375.04925061012-0.306009170095-0.637935919989-0.5598041897740.3486357734880.269636044623-0.04693697929860.2313555849180.1069311583680.02130849563920.189528471216-0.0430287536722-0.06934774927540.264001131305-0.006204701008450.23714287532-9.18366750772-22.597619196227.476898776
98.46773954821-0.4581084028170.7811386650516.888399176340.7958803796046.23572825295-0.316212978296-0.128066222299-0.0724593245898-0.3119133991730.196195945371-0.324708272686-0.382508487109-0.09172417180160.0939762236730.177834323702-0.023111106415-0.02022427826370.131034987017-0.08444416127020.2490052068988.83084410383-26.827524719111.2886904166
103.28870088449-4.16925124656-1.184204570566.577769212720.8299503141382.74128147106-0.226750995651-0.430646761645-0.5651397553160.1147863427620.288991223893-0.1751852927940.253452098074-0.0675063045914-0.01295409554390.175210339024-0.0383774481538-0.05280875407950.203387233324-0.05259386555820.2885865692330.583413227899-33.66617964414.3971793862
112.420345992712.88161798125-1.161128794555.13537727267-1.14963954647.57687897067-0.00691022130537-0.07482164675450.1158456078590.0315172072585-0.09396365294790.19150163269-0.1339396579930.015071093040.01443761853930.1306566341690.0444393536720.02775552280790.1014482582340.008478018937470.11589209406321.87522831587.68574905497-3.99553520712
125.055216461622.82446324635-4.053670551434.86732479457-2.267123085465.46648257789-0.1413106735310.270180774607-0.179367213639-0.649221903404-0.1343221529310.1783792586930.5997544664270.04104534800380.2225766548710.1653938731170.0474312108479-0.02923490121110.156358836243-0.03184313384970.14091033728222.06278703770.736274908157-12.1561398424
133.94811228470.687089522048-4.431932417813.72263800125-1.708424903097.112974650240.219889956038-0.183293687441-0.1424690731410.23229715635-0.383580845138-0.247385803061-0.1642809840280.6245259502150.03833009951230.09441801443790.02110033966980.002774628751470.1438429186370.05667589970870.15942502875728.1896738266-0.5321317329470.290337679358
144.72624339076-0.885104149273-1.414027590117.49458439649-0.3978441946896.339584437930.1197316733530.0735286338135-0.098034213803-0.219727690414-0.255400906773-0.3030036488710.2245229069630.353424627720.1506552525650.184633823739-0.002554415185240.04238172756830.1826555482360.03079997760790.090145886264316.99135998420.59210802514317.0296992114
151.168870120931.21370688618-0.943168649092.58315098017-2.443722344485.836915464360.162414171368-0.056025150127-0.009605715693170.316152760643-0.245076483221-0.138610973388-0.148903243780.116774347690.07945162953620.202401135452-0.01807813747970.0007865961522780.1236385341490.03045862596090.092940205765918.04742000356.1778040702520.0897385799
163.38347484914-0.0292004467129-0.4895559786772.95297981866-0.6600782038277.88553343652-0.05052459381390.174836856888-0.213485032438-0.3286065197060.04829844467680.09927949692410.111660777598-0.3751467819790.05752508032310.244466927423-0.0256682641273-0.02666277136040.174415893725-0.06001609384340.09795946695094.49263008689-15.073593063423.6421497379
173.831137636690.8529710699071.766680537177.80580968453-3.552861252433.302301068550.210100127283-0.103475031750.1380232157930.0081375655636-0.219111479730.756300494647-0.290578755254-0.3964952532170.1057438702490.226933218019-0.0225972531261-0.03289282013810.237432831003-0.05784379081640.14772478577-2.71928262378-10.93515197630.6273448043
183.150345278131.68146021251-1.198629336935.19010473048-1.330578724133.669100827530.03372179319740.0134267491636-0.331946727516-0.127777074309-0.190019764935-0.467132765890.2466958141610.0503771795393-0.1695909140680.227018045305-0.0832633954535-0.04904733443350.114732157885-0.0251489387642-0.011201567156612.4400658218-10.824276286327.3003717775
195.127227403790.965427410142-1.739331349155.06923393517-0.8426048442735.65438759717-0.09771238073850.111447153538-0.377365569837-0.206542517799-0.09009283927580.079652119570.331026405997-0.2088399706090.1152151220410.156876222665-0.007444197976970.02171837650420.107259970763-0.02514382727320.14811882278521.6589195424-12.39010454665.50103421972
208.08988337565-0.495359678662-3.836207811194.424290297640.557215364483.74275286181-0.0528165039682-0.521585447009-0.6865360692960.113678065648-0.286784776057-0.4482160846970.415926042570.4934175539010.1994367974150.216406477602-0.01244085437470.005036168500420.1173980675230.02826592393920.33278044694320.9234408059-20.488255110113.3494757493
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'E' and (resid 279 through 315 )EK279 - 3151 - 37
22chain 'E' and (resid 316 through 334 )EK316 - 33438 - 56
33chain 'F' and (resid 279 through 293 )FM279 - 2931 - 15
44chain 'F' and (resid 294 through 301 )FM294 - 30116 - 23
55chain 'F' and (resid 302 through 315 )FM302 - 31524 - 37
66chain 'F' and (resid 316 through 333 )FM316 - 33338 - 55
77chain 'G' and (resid 279 through 315 )GN279 - 3151 - 37
88chain 'G' and (resid 316 through 334 )GN316 - 33438 - 56
99chain 'H' and (resid 279 through 315 )HO279 - 3151 - 37
1010chain 'H' and (resid 316 through 334 )HO316 - 33438 - 56
1111chain 'A' and (resid 279 through 297 )AA279 - 2971 - 19
1212chain 'A' and (resid 298 through 315 )AA298 - 31520 - 37
1313chain 'A' and (resid 316 through 334 )AA316 - 33438 - 56
1414chain 'B' and (resid 279 through 301 )BD279 - 3011 - 23
1515chain 'B' and (resid 302 through 334 )BD302 - 33424 - 56
1616chain 'C' and (resid 279 through 301 )CE279 - 3011 - 23
1717chain 'C' and (resid 302 through 315 )CE302 - 31524 - 37
1818chain 'C' and (resid 316 through 334 )CE316 - 33438 - 56
1919chain 'D' and (resid 279 through 315 )DJ279 - 3151 - 37
2020chain 'D' and (resid 316 through 334 )DJ316 - 33438 - 56

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