Journal: To Be Published Title: Structure of a mouse IgG antibody antigen-binding fragment (Fab) targeting N6-methyladenosine (m6A), an RNA modification, m6A nucleoside ligand Authors: Aoki, S.T.
Mass: 24023.018 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Cell line (production host): HEK293 / Organ (production host): KIDNEY / Production host: Homo sapiens (human)
Mass: 23042.525 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Cell line (production host): HEK293 / Organ (production host): KIDNEY / Production host: Homo sapiens (human)
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.06→48.23 Å / SU ML: 0.3566 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 28.269 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 Details: Data anisotropic, eventually chose cut refinement cut off based on the "estimates of resolution limits in reciprocal lattice direction" by aimless where CC(1/2) > 0.3 in the anisotropic direction
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2531
1307
4.19 %
0.2744
Rwork
0.2238
29901
-
-
obs
0.225
31208
99.82 %
-
Solvent computation
Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL