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- PDB-8vd0: Human TCR ET650-4 in complex with DQ8-InsC8-15-IAPP2 -

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Basic information

Entry
Database: PDB / ID: 8vd0
TitleHuman TCR ET650-4 in complex with DQ8-InsC8-15-IAPP2
Components
  • (T-CELL-RECEPTOR, TCR ET650-4 ...) x 2
  • Hybrid insulin peptide (HIP; InsC8-15-IAPP74-80),MHC class II HLA-DQ-beta-1 chimera
  • MHC class II HLA-DQ-alpha chain
KeywordsIMMUNE SYSTEM / Immune T cell receptor-pMHC II complex
Function / homology
Function and homology information


antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / MHC class II protein complex / adaptive immune response / endosome membrane / lysosomal membrane
Similarity search - Function
MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain / Class II histocompatibility antigen, alpha domain / MHC class II, alpha/beta chain, N-terminal / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site ...MHC class II, beta chain, N-terminal / Class II histocompatibility antigen, beta domain / Class II histocompatibility antigen, beta domain / MHC class II, alpha chain, N-terminal / Class II histocompatibility antigen, alpha domain / Class II histocompatibility antigen, alpha domain / MHC class II, alpha/beta chain, N-terminal / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
PHOSPHATE ION / MHC class II HLA-DQ-beta-1 / MHC class II HLA-DQ-alpha chain
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å
AuthorsTran, T.M. / Lim, J.J. / Loh, T.Y. / Mannering, I.S. / Rossjohn, J. / Reid, H.H.
Funding support Australia, 2items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia)2008981 Australia
National Health and Medical Research Council (NHMRC, Australia)APP1123586 Australia
CitationJournal: J.Biol.Chem. / Year: 2024
Title: A structural basis of T cell cross-reactivity to native and spliced self-antigens presented by HLA-DQ8.
Authors: Tran, M.T. / Lim, J.J. / Loh, T.J. / Mannering, S.I. / Rossjohn, J. / Reid, H.H.
History
DepositionDec 14, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 7, 2024Provider: repository / Type: Initial release
Revision 1.1Aug 14, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Sep 4, 2024Group: Database references / Category: citation / Item: _citation.journal_volume
Revision 1.3Oct 30, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: MHC class II HLA-DQ-alpha chain
C: Hybrid insulin peptide (HIP; InsC8-15-IAPP74-80),MHC class II HLA-DQ-beta-1 chimera
F: MHC class II HLA-DQ-alpha chain
H: Hybrid insulin peptide (HIP; InsC8-15-IAPP74-80),MHC class II HLA-DQ-beta-1 chimera
D: T-CELL-RECEPTOR, TCR ET650-4 alpha
E: T-CELL-RECEPTOR, TCR ET650-4 beta
I: T-CELL-RECEPTOR, TCR ET650-4 alpha
J: T-CELL-RECEPTOR, TCR ET650-4 beta
hetero molecules


Theoretical massNumber of molelcules
Total (without water)194,13231
Polymers191,3518
Non-polymers2,78123
Water6,738374
1
A: MHC class II HLA-DQ-alpha chain
C: Hybrid insulin peptide (HIP; InsC8-15-IAPP74-80),MHC class II HLA-DQ-beta-1 chimera
D: T-CELL-RECEPTOR, TCR ET650-4 alpha
E: T-CELL-RECEPTOR, TCR ET650-4 beta
hetero molecules


Theoretical massNumber of molelcules
Total (without water)96,86614
Polymers95,6764
Non-polymers1,19110
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
F: MHC class II HLA-DQ-alpha chain
H: Hybrid insulin peptide (HIP; InsC8-15-IAPP74-80),MHC class II HLA-DQ-beta-1 chimera
I: T-CELL-RECEPTOR, TCR ET650-4 alpha
J: T-CELL-RECEPTOR, TCR ET650-4 beta
hetero molecules


Theoretical massNumber of molelcules
Total (without water)97,26617
Polymers95,6764
Non-polymers1,59013
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)72.193, 139.303, 120.495
Angle α, β, γ (deg.)90.000, 93.698, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 1 through 96 or (resid 97...
d_2ens_1(chain "F" and (resid 1 through 179 or resid 202 or resid 204 through 205))
d_1ens_2(chain "B" and (resid 3 through 103 or (resid 113...
d_2ens_2(chain "G" and (resid 3 through 21 or (resid 22...
d_1ens_3chain "C"
d_2ens_3(chain "H" and resid -2 through 13)
d_1ens_4(chain "D" and (resid 2 through 146 or (resid 147...
d_2ens_4(chain "I" and (resid 2 through 144 or (resid 145...
d_1ens_5(chain "E" and (resid 3 through 215 or (resid 216...
d_2ens_5(chain "J" and (resid 3 through 185 or (resid 186...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1ILEILEGLUGLUAA1 - 1793 - 182
d_12ens_1NAGNAGNAGNAGAI201
d_13ens_1GOLGOLGOLGOLAK203
d_14ens_1GOLGOLGOLGOLAL204
d_21ens_1ILEILEGLUGLUFC1 - 1793 - 182
d_22ens_1NAGNAGNAGNAGFP202
d_23ens_1GOLGOLGOLGOLFR204
d_24ens_1GOLGOLGOLGOLFS205
d_11ens_2SERSERARGARGCB21 - 20724 - 210
d_21ens_2SERSERARGARGHD21 - 20724 - 210
d_11ens_3GLYGLYGLYGLYCB-2 - 131 - 16
d_21ens_3GLYGLYGLYGLYHD-2 - 131 - 16
d_11ens_4METMETLYSLYSDE2 - 1971 - 180
d_12ens_4ASPASPSERSERDE199 - 219182 - 202
d_13ens_4GOLGOLGOLGOLDV301
d_21ens_4METMETSERSERIG2 - 2191 - 202
d_22ens_4GOLGOLGOLGOLIZ301
d_11ens_5GLYGLYASPASPEF3 - 2661 - 240
d_12ens_5GOLGOLGOLGOLEX301
d_21ens_5GLYGLYASPASPJH3 - 2661 - 240
d_22ens_5GOLGOLGOLGOLJCA301

NCS ensembles :
ID
ens_1
ens_2
ens_3
ens_4
ens_5

NCS oper:
IDCodeMatrixVector
1given(0.892212938115, -0.151955489872, 0.425282967162), (-0.169206364361, -0.985576575871, 0.00283184654439), (0.418718615923, -0.0744871948237, -0.905056008481)-21.345634003, 28.1064348397, 22.2294407684
2given(0.888739508085, -0.163668411854, 0.42819941351), (-0.183203583472, -0.983064731258, 0.00449234463936), (0.420212486454, -0.0824401911606, -0.903673105226)-21.2268351023, 28.2437611467, 22.0744421191
3given(0.888442774975, -0.164085284623, 0.428655403518), (-0.191790989578, -0.981190965154, 0.0219204520259), (0.416995985487, -0.101687311255, -0.903202102974)-21.2849393811, 28.7113609605, 22.20735766
4given(0.894102186793, -0.176117267313, 0.411781480552), (-0.211466127799, -0.976503437898, 0.0415103910705), (0.394795334782, -0.124192366622, -0.910336695793)-21.3551802869, 29.4425733139, 22.5280538531
5given(0.893616920645, -0.151418230331, 0.422517832359), (-0.188283814698, -0.981006000033, 0.0466522563504), (0.407428526567, -0.121242514915, -0.905153162905)-21.399437662, 29.154069438, 22.3484027263

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Components

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Protein , 2 types, 4 molecules AFCH

#1: Protein MHC class II HLA-DQ-alpha chain / DQA1*03:01 (DQ8-alpha chain


Mass: 21150.596 Da / Num. of mol.: 2 / Mutation: I75C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DQA1 / Plasmid: pZip3 / Cell line (production host): High Five (BTI-Tn-5B1-4) / Production host: Trichoplusia ni (cabbage looper) / Strain (production host): DH10B / References: UniProt: Q30069
#2: Protein Hybrid insulin peptide (HIP; InsC8-15-IAPP74-80),MHC class II HLA-DQ-beta-1 chimera


Mass: 24450.369 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pZip3 / Gene: HLA-DQB1 / Cell line (production host): High Five (BTI-Tn-5B1-4) / Production host: Trichoplusia ni (cabbage looper) / Strain (production host): DH10B / References: UniProt: O19707

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T-CELL-RECEPTOR, TCR ET650-4 ... , 2 types, 4 molecules DIEJ

#3: Protein T-CELL-RECEPTOR, TCR ET650-4 alpha


Mass: 22921.559 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Human CD4+ T cell clone isolated from PBMC of recent onset T1D patient
Source: (gene. exp.) Homo sapiens (human) / Gene: TRAV26-1*01 / Plasmid: pET30 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): Escherichia coli (E.coli)
#4: Protein T-CELL-RECEPTOR, TCR ET650-4 beta


Mass: 27153.135 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Human CD4+ T cell clone isolated from PBMC of recent onset T1D patient
Source: (gene. exp.) Homo sapiens (human) / Gene: TRBV5-1*01 / Plasmid: pET30 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): Escherichia coli (E.coli)

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Sugars , 1 types, 5 molecules

#5: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 3 types, 392 molecules

#6: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C3H8O3
#7: Chemical
ChemComp-PO4 / PHOSPHATE ION


Mass: 94.971 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: PO4
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 374 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.16 Å3/Da / Density % sol: 61.11 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 8
Details: 0.2 M K Na tartrate, 15% w/v PEG 20,000 with seeding and additive C8 silver bullet

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.95372 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Aug 6, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.95372 Å / Relative weight: 1
ReflectionResolution: 2.4→46.98 Å / Num. obs: 91516 / % possible obs: 98.7 % / Redundancy: 5 % / Biso Wilson estimate: 43.34 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.047 / Rrim(I) all: 0.078 / Net I/σ(I): 13.3
Reflection shellResolution: 2.4→2.44 Å / Rmerge(I) obs: 0.522 / Mean I/σ(I) obs: 3.2 / Num. unique obs: 4473 / CC1/2: 0.865 / Rpim(I) all: 0.258 / Rrim(I) all: 0.584

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Processing

Software
NameVersionClassification
PHENIX1.19_4092refinement
autoXDSdata reduction
Coot0.9.8.92model building
XDSccp4-8.0data scaling
PHASERccp4-8.0phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.4→45.51 Å / SU ML: 0.2715 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.0085
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.22 4596 5.03 %
Rwork0.1883 86860 -
obs0.1899 91456 98.6 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 52.96 Å2
Refinement stepCycle: LAST / Resolution: 2.4→45.51 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12736 0 172 374 13282
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002113216
X-RAY DIFFRACTIONf_angle_d0.517518006
X-RAY DIFFRACTIONf_chiral_restr0.04551995
X-RAY DIFFRACTIONf_plane_restr0.00422329
X-RAY DIFFRACTIONf_dihedral_angle_d12.09774663
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AAX-RAY DIFFRACTIONTorsion NCS3.89707972665
ens_2d_2BCX-RAY DIFFRACTIONTorsion NCS0.53277651086
ens_3d_2CFX-RAY DIFFRACTIONTorsion NCS0.328478891329
ens_4d_2GIX-RAY DIFFRACTIONTorsion NCS2.98226043605
ens_5d_2HJX-RAY DIFFRACTIONTorsion NCS1.51042414024
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.4-2.430.30211430.24432843X-RAY DIFFRACTION98.13
2.43-2.460.27681570.25472886X-RAY DIFFRACTION98.19
2.46-2.490.31421480.2342894X-RAY DIFFRACTION97.44
2.49-2.520.27251550.23492804X-RAY DIFFRACTION98.4
2.52-2.550.27811590.22432868X-RAY DIFFRACTION98.18
2.55-2.590.29511430.21912883X-RAY DIFFRACTION97.49
2.59-2.620.26551600.24112899X-RAY DIFFRACTION98.74
2.62-2.660.27671320.25182852X-RAY DIFFRACTION97.9
2.66-2.70.29151880.25992836X-RAY DIFFRACTION98.15
2.7-2.750.35811580.25752922X-RAY DIFFRACTION98.56
2.75-2.790.30081400.24372870X-RAY DIFFRACTION98.01
2.79-2.850.27931730.22942858X-RAY DIFFRACTION98.79
2.85-2.90.27851480.22492904X-RAY DIFFRACTION97.82
2.9-2.960.25341540.20832874X-RAY DIFFRACTION98.86
2.96-3.020.24621290.20692916X-RAY DIFFRACTION98.29
3.02-3.090.23251580.21392896X-RAY DIFFRACTION98.9
3.09-3.170.23321590.21442879X-RAY DIFFRACTION98.54
3.17-3.260.28891580.21722887X-RAY DIFFRACTION98.74
3.26-3.350.23571490.21592905X-RAY DIFFRACTION98.9
3.35-3.460.25141380.19852914X-RAY DIFFRACTION98.67
3.46-3.580.23331470.18442907X-RAY DIFFRACTION98.83
3.58-3.730.20531480.18252902X-RAY DIFFRACTION99.15
3.73-3.90.20771440.17272924X-RAY DIFFRACTION99.03
3.9-4.10.17811820.16972883X-RAY DIFFRACTION99.09
4.1-4.360.1751400.14492934X-RAY DIFFRACTION99.1
4.36-4.70.15741750.13662886X-RAY DIFFRACTION99.16
4.7-5.170.17531470.142960X-RAY DIFFRACTION99.11
5.17-5.910.19711360.16722957X-RAY DIFFRACTION99.49
5.92-7.440.20411670.1882935X-RAY DIFFRACTION99.33
7.45-45.510.17141610.17142982X-RAY DIFFRACTION99.15
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.18879574634-0.3016735252932.143787617841.31359223721-0.01726438706613.352565999960.00182067457815-0.329958111204-0.206933213290.238286261420.0914530847973-0.009654034282220.2683892675040.140228213575-0.09150116476140.3652899294950.0242956687066-0.09526354754260.4067436830720.01548175691560.24989419233117.268-5.592-1.869
21.32838648930.235568449179-0.009347065748971.98577887680.5897606801232.285559104830.0595757578514-0.4454785697840.2016002538640.12680789572-0.0381752841597-0.0280452654164-0.360232253064-0.0318559366546-0.0573126696880.359232411368-0.0172940368204-0.06228526628070.294699523688-0.0001570933531550.2775973457069.2180.995-0.733
34.036541797821.306911085231.485312144733.21322980560.6159484159377.04450422265-0.0610097098899-0.5749219415880.8951590258530.136438356651-0.2172490557130.277505284924-0.961797079253-0.1332315277990.2247520264450.46498023719-0.01356393492840.005845233433310.362801783167-0.1026653916950.40725103385712.62911.6250.961
41.22572566880.504552070872-0.2080525486781.700813894120.9307214358022.31523929384-0.06971523371420.304647379355-0.209226653688-0.1440040301740.0617754021820.1187504651460.479428165536-0.164085001490.03600023171130.36830925549-0.0199349290138-0.07197822501680.313662650921-0.03351021677370.3510595407154.08-7.401-12.044
51.99642272062-0.551881672224-0.4383427842261.18623296170.2951680893323.14366219028-0.0365477318898-0.252151302261-0.09933368406870.284234437679-0.06181160194790.2484387886770.222928882921-0.1437268541680.07989042401670.405941678488-0.05090405028180.01574017709080.286556692722-0.005576913533110.3452323422132.289-3.31415.431
61.416622121650.591153255055-1.892134772343.331356451541.485316955626.221277421330.166659165070.02824032863220.0301135210660.6942289932-0.4200505667480.2399007622731.37160823069-1.106885118690.1074130376780.555966246971-0.1235843088420.04568061513480.3847772798460.001786088470970.387492360935-4.002-10.1315.63
72.498768374850.4432624772480.2966107897871.656190984650.7017251828462.593977106390.00835600914591-0.176509715009-0.1598973103760.0592748529717-0.05189826917010.005327502730940.2731844493810.02948072651150.02872019118020.3144210029150.0139507274424-0.05023812337420.21359842972-0.0009157207784820.23298149003110.86-8.278-5.759
82.4973825635-0.373815592184-1.056893478642.16416037665-0.3341140293562.4474963010.109815178032-0.044135408482-0.1127511953530.0828501477512-0.0995944244535-0.1516669047810.2304174660090.259722701306-0.02889475235480.3565037819920.0439753293197-0.02470965576040.267508269753-0.0004013387198960.27584249723619.412-11.725-10.34
95.872827370034.53246441605-3.458198979864.44343434581-4.45447987385.31577408524-0.3702574921460.530777929356-0.0909413136017-1.082398482330.15496700437-0.3531918353820.204261291026-0.6950852917510.2169422021810.4838589626070.0717946988149-0.06220891311210.426618620543-0.08881325479020.30258238143616.83-10.787-23.377
103.18545028511-0.2083129789621.218652920855.210372577811.768927606492.741777864360.08169510231610.1671770701860.179579887878-0.464940028587-0.00605403563203-0.364230638358-0.3845507902440.101486547912-0.06228480415440.3109790300260.06799438493590.001636805577110.4036602747740.01049983562070.29550639181625.199-1.047-17.668
113.88349626505-1.851055313-0.996508250121.713871103390.634462048422.138350893990.124988166896-0.638692875743-0.0439341674436-0.2184569167020.214949487190.0724553218241-0.6366257833921.34644846584-0.2799784255310.353003469333-0.077503107716-0.04668244478350.600702885-0.01492881377180.3903941703327.7545.252-1.622
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Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 0:14 )A0 - 14
2X-RAY DIFFRACTION2( CHAIN A AND RESID 15:35 )A15 - 35
3X-RAY DIFFRACTION3( CHAIN A AND RESID 36:55 )A36 - 55
4X-RAY DIFFRACTION4( CHAIN A AND RESID 56:87 )A56 - 87
5X-RAY DIFFRACTION5( CHAIN A AND RESID 88:160 )A88 - 160
6X-RAY DIFFRACTION6( CHAIN A AND RESID 161:181 )A161 - 181
7X-RAY DIFFRACTION7( CHAIN C AND RESID 21:36 )C21 - 36
8X-RAY DIFFRACTION8( CHAIN C AND RESID 37:72 )C37 - 72
9X-RAY DIFFRACTION9( CHAIN C AND RESID 73:82 )C73 - 82
10X-RAY DIFFRACTION10( CHAIN C AND RESID 83:95 )C83 - 95
11X-RAY DIFFRACTION11( CHAIN C AND RESID 96:106 )C96 - 106
12X-RAY DIFFRACTION12( CHAIN C AND RESID 107:115 )C107 - 115
13X-RAY DIFFRACTION13( CHAIN C AND RESID 116:151 )C116 - 151
14X-RAY DIFFRACTION14( CHAIN C AND RESID 152:162 )C152 - 162
15X-RAY DIFFRACTION15( CHAIN C AND RESID 163:195 )C163 - 195
16X-RAY DIFFRACTION16( CHAIN C AND RESID 196:207 )C196 - 207
17X-RAY DIFFRACTION17( CHAIN C AND RESID -2:2 )C-2 - 2
18X-RAY DIFFRACTION18( CHAIN C AND RESID 3:7 )C3 - 7
19X-RAY DIFFRACTION19( CHAIN C AND RESID 8:13 )C8 - 13
20X-RAY DIFFRACTION20( CHAIN F AND RESID 1:26 )F1 - 26
21X-RAY DIFFRACTION21( CHAIN F AND RESID 27:55 )F27 - 55
22X-RAY DIFFRACTION22( CHAIN F AND RESID 56:76 )F56 - 76
23X-RAY DIFFRACTION23( CHAIN F AND RESID 77:87 )F77 - 87
24X-RAY DIFFRACTION24( CHAIN F AND RESID 88:101 )F88 - 101
25X-RAY DIFFRACTION25( CHAIN F AND RESID 102:123 )F102 - 123
26X-RAY DIFFRACTION26( CHAIN F AND RESID 124:134 )F124 - 134
27X-RAY DIFFRACTION27( CHAIN F AND RESID 135:179 )F135 - 179
28X-RAY DIFFRACTION28( CHAIN H AND RESID 21:36 )H21 - 36
29X-RAY DIFFRACTION29( CHAIN H AND RESID 37:50 )H37 - 50
30X-RAY DIFFRACTION30( CHAIN H AND RESID 51:72 )H51 - 72
31X-RAY DIFFRACTION31( CHAIN H AND RESID 73:82 )H73 - 82
32X-RAY DIFFRACTION32( CHAIN H AND RESID 83:106 )H83 - 106
33X-RAY DIFFRACTION33( CHAIN H AND RESID 107:121 )H107 - 121
34X-RAY DIFFRACTION34( CHAIN H AND RESID 131:151 )H131 - 151
35X-RAY DIFFRACTION35( CHAIN H AND RESID 152:162 )H152 - 162
36X-RAY DIFFRACTION36( CHAIN H AND RESID 163:181 )H163 - 181
37X-RAY DIFFRACTION37( CHAIN H AND RESID 182:195 )H182 - 195
38X-RAY DIFFRACTION38( CHAIN H AND RESID 196:207 )H196 - 207
39X-RAY DIFFRACTION39( CHAIN H AND RESID -2:7 )H-2 - 7
40X-RAY DIFFRACTION40( CHAIN H AND RESID 8:12 )H8 - 12
41X-RAY DIFFRACTION41( CHAIN H AND RESID 13:14 )H13 - 14
42X-RAY DIFFRACTION42( CHAIN D AND RESID 2:37 )D2 - 37
43X-RAY DIFFRACTION43( CHAIN D AND RESID 38:122 )D38 - 122
44X-RAY DIFFRACTION44( CHAIN D AND RESID 123:170 )D123 - 170
45X-RAY DIFFRACTION45( CHAIN D AND RESID 171:219 )D171 - 219
46X-RAY DIFFRACTION46( CHAIN E AND RESID 3:24 )E3 - 24
47X-RAY DIFFRACTION47( CHAIN E AND RESID 25:119 )E25 - 119
48X-RAY DIFFRACTION48( CHAIN E AND RESID 120:134 )E120 - 134
49X-RAY DIFFRACTION49( CHAIN E AND RESID 135:213 )E135 - 213
50X-RAY DIFFRACTION50( CHAIN E AND RESID 214:266 )E214 - 266
51X-RAY DIFFRACTION51( CHAIN I AND RESID 2:37 )I2 - 37
52X-RAY DIFFRACTION52( CHAIN I AND RESID 38:91 )I38 - 91
53X-RAY DIFFRACTION53( CHAIN I AND RESID 92:155 )I92 - 155
54X-RAY DIFFRACTION54( CHAIN I AND RESID 156:219 )I156 - 219
55X-RAY DIFFRACTION55( CHAIN J AND RESID 3:119 )J3 - 119
56X-RAY DIFFRACTION56( CHAIN J AND RESID 120:150 )J120 - 150
57X-RAY DIFFRACTION57( CHAIN J AND RESID 151:198 )J151 - 198
58X-RAY DIFFRACTION58( CHAIN J AND RESID 199:266 )J199 - 266

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