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- PDB-8utf: Structure of the Measles virus Fusion protein in the post-fusion ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8utf | ||||||||||||||||||
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Title | Structure of the Measles virus Fusion protein in the post-fusion conformation | ||||||||||||||||||
![]() | (Fusion glycoprotein F0) x 2 | ||||||||||||||||||
![]() | VIRAL PROTEIN / glycoprotein / immune system / measles / high-resolution / ectodomain / post-fusion | ||||||||||||||||||
Function / homology | Precursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0 / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / viral envelope / host cell plasma membrane / virion membrane / membrane / Fusion glycoprotein F0![]() | ||||||||||||||||||
Biological species | ![]() | ||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.73 Å | ||||||||||||||||||
![]() | Zyla, D. / Saphire, E.O. | ||||||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: A neutralizing antibody prevents postfusion transition of measles virus fusion protein Authors: Zyla, D.S. / Della Marca, R. / Niemeyer, G. / Zipursky, G. / Stearns, K. / Leedale, C. / Sobolik, E.B. / Callaway, H.M. / Hariharan, C. / Peng, W. / Parekh, D. / Marcink, T.C. / Diaz Avalos, ...Authors: Zyla, D.S. / Della Marca, R. / Niemeyer, G. / Zipursky, G. / Stearns, K. / Leedale, C. / Sobolik, E.B. / Callaway, H.M. / Hariharan, C. / Peng, W. / Parekh, D. / Marcink, T.C. / Diaz Avalos, R. / Horvat, B. / Mathieu, C. / Snijder, J. / Greninger, A.L. / Hastie, K.M. / Niewiesk, S. / Moscona, A. / Porotto, M. / Saphire, E.O. | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 312.4 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 43.9 KB | Display | |
Data in CIF | ![]() | 65.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 42539MC ![]() 8ut2C ![]() 8uupC ![]() 8uuqC ![]() 9at8C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 12498.768 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 44898.820 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Sugar | ChemComp-NAG / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Structure of the Measles virus Fusion protein in the post-fusion conformation Type: COMPLEX / Details: Spontaneously sprung from the pre-fusion state / Entity ID: #1-#2 / Source: RECOMBINANT |
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Molecular weight | Units: MEGADALTONS / Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8 / Details: HEPES 50 mM, pH 8.0, NaCl 150 mM |
Specimen | Conc.: 0.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Specimen support | Grid material: COPPER / Grid mesh size: 400 divisions/in. / Grid type: Quantifoil R1.2/1.3 |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 298 K |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1000 nm |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Average exposure time: 2 sec. / Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) / Num. of grids imaged: 3 / Num. of real images: 6028 |
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Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Particle selection | Num. of particles selected: 900000 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 2.73 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 158000 / Num. of class averages: 1 / Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Atomic model building | Protocol: RIGID BODY FIT / Space: REAL | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Atomic model building | Source name: SwissModel / Type: in silico model | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 81.61 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
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