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Yorodumi- PDB-8us4: C2221 Crystal structure of TamA (Barrel only) from Pseudomonas ae... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8us4 | ||||||
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Title | C2221 Crystal structure of TamA (Barrel only) from Pseudomonas aeruginosa at 3.15 Ang | ||||||
Components | Translocation and assembly module subunit TamA | ||||||
Keywords | MEMBRANE PROTEIN / Omp85 / protein translocation | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa PAO1 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.15 Å | ||||||
Authors | Mellouk, A. / Moraes, T.F. / Calmettes, C. | ||||||
Funding support | Canada, 1items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2024 Title: POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties. Authors: Mellouk, A. / Jaouen, P. / Ruel, L.J. / Le, M. / Martini, C. / Moraes, T.F. / El Bakkouri, M. / Lague, P. / Boisselier, E. / Calmettes, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8us4.cif.gz | 73.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8us4.ent.gz | 53.4 KB | Display | PDB format |
PDBx/mmJSON format | 8us4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8us4_validation.pdf.gz | 427.6 KB | Display | wwPDB validaton report |
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Full document | 8us4_full_validation.pdf.gz | 431.8 KB | Display | |
Data in XML | 8us4_validation.xml.gz | 13.4 KB | Display | |
Data in CIF | 8us4_validation.cif.gz | 17.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/us/8us4 ftp://data.pdbj.org/pub/pdb/validation_reports/us/8us4 | HTTPS FTP |
-Related structure data
Related structure data | 8us1C 8us2C 8us3C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34052.016 Da / Num. of mol.: 1 / Fragment: Mature TamA Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa PAO1 (bacteria) Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: PA2543 / Plasmid: pET / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): C43 / References: UniProt: Q9I0U1 | ||
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#2: Chemical | ChemComp-PT / Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.44 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 12% peg 4K, 50 mM MgCl2, 70 mM Hepes pH 7.2, 4% isopropanol, 10% glycerol |
-Data collection
Diffraction | Mean temperature: 77 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.97910 Ang | ||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 12, 2013 | ||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.15→48.988 Å / Num. obs: 12152 / % possible obs: 97.8 % / Redundancy: 3.9 % / Biso Wilson estimate: 88.521 Å2 / Rpim(I) all: 0.121 / Rrim(I) all: 0.244 / Rsym value: 0.168 / Net I/av σ(I): 1.996 / Net I/σ(I): 7.5 | ||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.15→48.987 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso max: 226.53 Å2 / Biso mean: 90.0774 Å2 / Biso min: 47.35 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.15→48.987 Å
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