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Yorodumi- PDB-8us1: P21 Crystal structure of TamA from Pseudomonas aeruginosa at 2.6 Ang -
+Open data
-Basic information
Entry | Database: PDB / ID: 8us1 | ||||||
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Title | P21 Crystal structure of TamA from Pseudomonas aeruginosa at 2.6 Ang | ||||||
Components | Translocation and assembly module subunit TamA | ||||||
Keywords | MEMBRANE PROTEIN / Omp85 / protein translocation | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.65 Å | ||||||
Authors | Mellouk, A. / Moraes, T.F. / Calmettes, C. | ||||||
Funding support | Canada, 1items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2024 Title: POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties Authors: Mellouk, A. / Jaouen, P.L. / Ruel, L.J. / Le, M. / Martini, C. / Moraes, T.F. / El Bakkouri, M. / Lague, P. / Boisselier, E. / Calmettes, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8us1.cif.gz | 234.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8us1.ent.gz | 187.1 KB | Display | PDB format |
PDBx/mmJSON format | 8us1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8us1_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 8us1_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 8us1_validation.xml.gz | 23.6 KB | Display | |
Data in CIF | 8us1_validation.cif.gz | 32.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/us/8us1 ftp://data.pdbj.org/pub/pdb/validation_reports/us/8us1 | HTTPS FTP |
-Related structure data
Related structure data | 8us2C 8us3C 8us4C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 61980.215 Da / Num. of mol.: 1 / Fragment: Mature TamA Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: PA2543 / Plasmid: pET / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): C43 / References: UniProt: Q9I0U1 | ||||
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#2: Chemical | ChemComp-C8E / ( #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.23 Å3/Da / Density % sol: 61.9 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 12% peg 4K, 50 mM MgCl2, 70 mM Hepes pH 7.2, 4% isopropanol, 10% glycerol |
-Data collection
Diffraction | Mean temperature: 77 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.97910 Ang | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 7, 2013 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.65→50 Å / Num. obs: 41274 / % possible obs: 93.2 % / Redundancy: 4.1 % / Biso Wilson estimate: 41.982 Å2 / Rmerge(I) obs: 0.115 / Χ2: 3.284 / Net I/av σ(I): 16.11 / Net I/σ(I): 12.6 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.65→40.9 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso max: 238.34 Å2 / Biso mean: 50.0506 Å2 / Biso min: 17.44 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.65→40.9 Å
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