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- PDB-8us3: C2 Crystal structure of TamA from Pseudomonas aeruginosa at 3.1 Ang -
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Open data
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Basic information
Entry | Database: PDB / ID: 8us3 | ||||||
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Title | C2 Crystal structure of TamA from Pseudomonas aeruginosa at 3.1 Ang | ||||||
![]() | Translocation and assembly module subunit TamA | ||||||
![]() | MEMBRANE PROTEIN / Omp85 / protein translocation | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mellouk, A. / Moraes, T.F. / Calmettes, C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties Authors: Mellouk, A. / Jaouen, P.L. / Ruel, L.J. / Le, M. / Martini, C. / Moraes, T.F. / El Bakkouri, M. / Lague, P. / Boisselier, E. / Calmettes, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 235.1 KB | Display | ![]() |
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PDB format | ![]() | 190 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 578.7 KB | Display | ![]() |
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Full document | ![]() | 591.5 KB | Display | |
Data in XML | ![]() | 22.7 KB | Display | |
Data in CIF | ![]() | 30.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8us1C ![]() 8us2C ![]() 8us4C C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 61651.949 Da / Num. of mol.: 1 / Fragment: Mature TamA Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: PA2543 / Plasmid: pET / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-C8E / ( |
#3: Chemical | ChemComp-MG / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.87 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 12% peg 4K, 50 mM MgCl2, 70 mM Hepes pH 7.2, 4% isopropanol, 10% glycerol |
-Data collection
Diffraction | Mean temperature: 77 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 12, 2013 | ||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 | ||||||||||||||||||||||||
Reflection | Resolution: 3.1→48.6 Å / Num. obs: 13130 / % possible obs: 100 % / Redundancy: 2 % / Biso Wilson estimate: 68.282 Å2 / CC1/2: 0.9 / Rsym value: 0.04 / Net I/σ(I): 11.2 | ||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso max: 214.1 Å2 / Biso min: 18.74 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→48.6 Å
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