- PDB-8u0g: Full-length dimer of DNA-Damage Response Protein C from Deinococc... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 8u0g
タイトル
Full-length dimer of DNA-Damage Response Protein C from Deinococcus radiodurans - Crystal form xMJ7124
要素
DNA damage response protein C
キーワード
DNA BINDING PROTEIN / DNA Repair / Radioresistance
機能・相同性
cellular response to desiccation / nucleoid / cellular response to gamma radiation / DNA repair / DNA damage response / cytoplasm / DNA damage response protein C
分子量: 25241.369 Da / 分子数: 2 / 由来タイプ: 組換発現 由来: (組換発現) Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 (放射線耐性) 株: R1 / 遺伝子: ddrC / プラスミド: pMJ5741 詳細 (発現宿主): WT DdrC with N-term His-tag and TEV site in pDEST-527 backbone 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): (DE3)-T1R / 参照: UniProt: Q9RYE6
-
実験情報
-
実験
実験
手法: X線回折 / 使用した結晶の数: 1
-
試料調製
結晶
マシュー密度: 3.48 Å3/Da / 溶媒含有率: 64.6 % 解説: Hexagonal bipyramid ~100um in width, depth and height
結晶化
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: 1.5 ul of protein solution was mixed with 1.5 uL of crystallization solution and hung upside-down in a sealed chamber containing 1mL of well solution Protein solution: 140uM DdrC, 200mM ...詳細: 1.5 ul of protein solution was mixed with 1.5 uL of crystallization solution and hung upside-down in a sealed chamber containing 1mL of well solution Protein solution: 140uM DdrC, 200mM Sodium sulfate, 1mM Magnesium chloride, 20mM Sodium citrate / Citric acid, pH 6.5 Crystallization solution (Wizard Classics 1 #22) 10% (v/v) 2-propanol 100mM Tris-base/HCl, pH 8.5 Well solution: 1.5M Ammonium sulfate PH範囲: 6.0 - 8.5 / Temp details: Temperature-controlled incubator at 20C
-
データ収集
回折
平均測定温度: 100 K / Ambient temp details: Nitrogen cryo-stream / Serial crystal experiment: N