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- PDB-8trv: Structure of the EphA2 LBDCRD bound to FabS1C_C1 -

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Basic information

Entry
Database: PDB / ID: 8trv
TitleStructure of the EphA2 LBDCRD bound to FabS1C_C1
Components
  • (S1C variant of Fab_C1 ...) x 2
  • Ephrin type-A receptor 2
KeywordsSIGNALING PROTEIN/IMMUNE SYSTEM / Cell signalling / antibody / agonist / high affinity / clustering / activation / SIGNALING PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


notochord cell development / notochord formation / blood vessel endothelial cell proliferation involved in sprouting angiogenesis / lens fiber cell morphogenesis / negative regulation of lymphangiogenesis / axial mesoderm formation / cAMP metabolic process / regulation of blood vessel endothelial cell migration / pericyte cell differentiation / leading edge membrane ...notochord cell development / notochord formation / blood vessel endothelial cell proliferation involved in sprouting angiogenesis / lens fiber cell morphogenesis / negative regulation of lymphangiogenesis / axial mesoderm formation / cAMP metabolic process / regulation of blood vessel endothelial cell migration / pericyte cell differentiation / leading edge membrane / negative regulation of chemokine production / response to growth factor / post-anal tail morphogenesis / activation of GTPase activity / bone remodeling / positive regulation of bicellular tight junction assembly / regulation of lamellipodium assembly / branching involved in mammary gland duct morphogenesis / EPH-Ephrin signaling / tight junction / RND1 GTPase cycle / RND2 GTPase cycle / RND3 GTPase cycle / neural tube development / mammary gland epithelial cell proliferation / RHOV GTPase cycle / EPHA-mediated growth cone collapse / growth factor binding / regulation of cell adhesion mediated by integrin / hepatocyte growth factor receptor activity / platelet-derived growth factor beta-receptor activity / placental growth factor receptor activity / brain-derived neurotrophic factor receptor activity / boss receptor activity / platelet-derived growth factor alpha-receptor activity / epidermal growth factor receptor activity / macrophage colony-stimulating factor receptor activity / RHOU GTPase cycle / protein tyrosine kinase collagen receptor activity / lamellipodium membrane / stem cell factor receptor activity / insulin-like growth factor receptor activity / transmembrane-ephrin receptor activity / RHOG GTPase cycle / EPH-ephrin mediated repulsion of cells / GPI-linked ephrin receptor activity / vascular endothelial growth factor receptor activity / RAC2 GTPase cycle / RAC3 GTPase cycle / regulation of angiogenesis / fibroblast growth factor receptor activity / ephrin receptor signaling pathway / insulin receptor activity / regulation of ERK1 and ERK2 cascade / vasculogenesis / keratinocyte differentiation / RAC1 GTPase cycle / transmembrane receptor protein tyrosine kinase activity / cell surface receptor protein tyrosine kinase signaling pathway / osteoclast differentiation / negative regulation of angiogenesis / molecular function activator activity / skeletal system development / protein localization to plasma membrane / cell chemotaxis / positive regulation of protein localization to plasma membrane / cell motility / receptor protein-tyrosine kinase / ruffle membrane / intrinsic apoptotic signaling pathway in response to DNA damage / neuron differentiation / osteoblast differentiation / cell migration / lamellipodium / virus receptor activity / angiogenesis / receptor complex / cell adhesion / defense response to Gram-positive bacterium / positive regulation of cell migration / cadherin binding / inflammatory response / focal adhesion / cell surface / ATP binding / plasma membrane
Similarity search - Function
Ephrin type-A receptor 2, ligand binding domain / Ephrin receptor type-A /type-B / Ephrin receptor ligand binding domain / Tyrosine-protein kinase, receptor class V, conserved site / Ephrin receptor, transmembrane domain / : / Ephrin receptor ligand binding domain / Ephrin type-A receptor 2 transmembrane domain / Receptor tyrosine kinase class V signature 1. / Receptor tyrosine kinase class V signature 2. ...Ephrin type-A receptor 2, ligand binding domain / Ephrin receptor type-A /type-B / Ephrin receptor ligand binding domain / Tyrosine-protein kinase, receptor class V, conserved site / Ephrin receptor, transmembrane domain / : / Ephrin receptor ligand binding domain / Ephrin type-A receptor 2 transmembrane domain / Receptor tyrosine kinase class V signature 1. / Receptor tyrosine kinase class V signature 2. / Eph receptor ligand-binding domain profile. / Ephrin receptor ligand binding domain / Putative ephrin-receptor like / SAM domain (Sterile alpha motif) / SAM domain profile. / Sterile alpha motif. / Sterile alpha motif domain / Sterile alpha motif/pointed domain superfamily / Growth factor receptor cysteine-rich domain superfamily / Fibronectin type III domain / Fibronectin type 3 domain / Fibronectin type-III domain profile. / Galactose-binding-like domain superfamily / Fibronectin type III / Fibronectin type III superfamily / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Immunoglobulin-like fold / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
IODIDE ION / Ephrin type-A receptor 2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.25 Å
AuthorsSinger, A.U. / Bruce, H.A. / Blazer, L. / Adams, J.J. / Sicheri, F. / Sidhu, S.S.
Funding support United States, 2items
OrganizationGrant numberCountry
Celgene CorporationOperating Contract United States
Bristol-Myers Squibb (BMS) ResearchCollaboration and Option Agreement United States
CitationJournal: Protein Sci. / Year: 2025
Title: Synthetic antibodies targeting EphA2 induce diverse signaling-competent clusters with differential activation.
Authors: Adams, J.J. / Bruce, H.A. / Subramania, S. / Ploder, L. / Garcia, J. / Pot, I. / Blazer, L.L. / Singer, A.U. / Sidhu, S.S.
History
DepositionAug 10, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 17, 2024Provider: repository / Type: Initial release
Revision 1.1Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification
Revision 1.2May 7, 2025Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.title / _citation.year
Revision 1.3Jun 4, 2025Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ephrin type-A receptor 2
C: S1C variant of Fab_C1 heavy chain
G: S1C variant of Fab_C1 light chain
D: S1C variant of Fab_C1 heavy chain
E: S1C variant of Fab_C1 light chain
B: Ephrin type-A receptor 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)163,54326
Polymers162,4006
Non-polymers1,14320
Water00
1
A: Ephrin type-A receptor 2
C: S1C variant of Fab_C1 heavy chain
G: S1C variant of Fab_C1 light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)81,66412
Polymers81,2003
Non-polymers4659
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: S1C variant of Fab_C1 heavy chain
E: S1C variant of Fab_C1 light chain
B: Ephrin type-A receptor 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)81,87814
Polymers81,2003
Non-polymers67811
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)73.933, 110.558, 263.443
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21221
Space group name HallP22ab(y,z,x)
Symmetry operation#1: x,y,z
#2: x+1/2,-y,-z+1/2
#3: -x,y,-z
#4: -x+1/2,-y,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and ((resid 27 through 28 and (name N...
d_2ens_1(chain "B" and (resid 27 through 43 or (resid 44...
d_1ens_2(chain "C" and (resid 1 through 30 or (resid 31...
d_2ens_2(chain "D" and (resid 1 through 61 or (resid 62...
d_1ens_3(chain "E" and (resid 1 through 162 or (resid 163...
d_2ens_3(chain "G" and (resid 1 through 2 or (resid 3...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1LYSLYSMETMETAA27 - 595 - 37
d_12ens_1PROPROSERSERAA63 - 7441 - 52
d_13ens_1ASPASPPHEPHEAA78 - 10856 - 86
d_14ens_1CYSCYSLYSLYSAA115 - 11693 - 94
d_15ens_1THRTHRVALVALAA118 - 15296 - 130
d_16ens_1LEULEUALAALAAA163 - 216141 - 194
d_17ens_1SERSERPROPROAA218 - 329196 - 307
d_21ens_1LYSLYSMETMETBF27 - 595 - 37
d_22ens_1PROPROSERSERBF63 - 6841 - 46
d_23ens_1CYSCYSPHEPHEBF70 - 10848 - 86
d_24ens_1CYSCYSPROPROBF115 - 32993 - 307
d_11ens_2GLUGLUSERSERCB1 - 2391 - 217
d_21ens_2GLUGLUSERSERDD1 - 2391 - 217
d_11ens_3ASPASPCYSCYSEE1 - 2321 - 215
d_21ens_3ASPASPCYSCYSGC1 - 2321 - 215

NCS ensembles :
ID
ens_1
ens_2
ens_3

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Ephrin type-A receptor 2 / Epithelial cell kinase / Tyrosine-protein kinase receptor ECK


Mass: 33952.336 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: residues 27-330 / Source: (gene. exp.) Homo sapiens (human) / Gene: EPHA2, ECK / Plasmid: pSCSTa
Details (production host): expressed with a C-terminal thrombin cleavage site and 6-his sequence. The residues LTPR at the C-terminus are derived from the thrombin cleavage site
Cell line (production host): Expi293 / Production host: Homo sapiens (human)
References: UniProt: P29317, receptor protein-tyrosine kinase

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Antibody , 2 types, 4 molecules CDGE

#2: Antibody S1C variant of Fab_C1 heavy chain


Mass: 23814.611 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Fab produced by randomization of CDR regions and selected by phage display. Fabs utilize IMGT (LeClerc et al., Dev Comp Immunol. 2003 Jan;27(1):55-77) numbering.
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pSCSTa / Cell line (production host): Expi293 / Production host: Homo sapiens (human)
#3: Antibody S1C variant of Fab_C1 light chain


Mass: 23432.895 Da / Num. of mol.: 2 / Mutation: SPHAGLSSP replaced by QGTTS; Q165S, K167Y
Source method: isolated from a genetically manipulated source
Details: Fab produced by randomization of CDR regions and selected by phage display. Fabs utilize IMGT (LeClerc et al., Dev Comp Immunol. 2003 Jan;27(1):55-77) numbering.
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pSCSTa / Cell line (production host): Expi293 / Production host: Homo sapiens (human)

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Non-polymers , 4 types, 20 molecules

#4: Chemical
ChemComp-IOD / IODIDE ION


Mass: 126.904 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: I / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: Cl / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Na / Feature type: SUBJECT OF INVESTIGATION
#7: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2 / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.31 Å3/Da / Density % sol: 62.89 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop
Details: 20% PEG3350, 200 mM sodium iodide (PACT Premiere solution E3)
Temp details: room temperature

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 5, 2022 / Details: Mirrors
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.99→131.72 Å / Num. obs: 44485 / % possible obs: 99.6 % / Redundancy: 6.7 % / Biso Wilson estimate: 103.88 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.121 / Rpim(I) all: 0.05 / Rrim(I) all: 0.132 / Net I/σ(I): 12
Reflection shellResolution: 2.99→3.1 Å / Redundancy: 5.9 % / Rmerge(I) obs: 2.214 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 4523 / CC1/2: 0.349 / Rpim(I) all: 0.975 / Rrim(I) all: 2.427 / % possible all: 98.6

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
PHENIX1.20.1_4487refinement
X-Areadata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entries 3FL7 & 8TRS

3fl7

8trs


Resolution: 3.25→131.72 Å / SU ML: 0.4573 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.7391
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.272 1683 4.84 %
Rwork0.217 33088 -
obs0.2197 34771 99.57 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 123.87 Å2
Refinement stepCycle: LAST / Resolution: 3.25→131.72 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10669 0 23 0 10692
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.010110933
X-RAY DIFFRACTIONf_angle_d1.161414926
X-RAY DIFFRACTIONf_chiral_restr0.06271678
X-RAY DIFFRACTIONf_plane_restr0.01331934
X-RAY DIFFRACTIONf_dihedral_angle_d16.52833744
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AAX-RAY DIFFRACTIONTorsion NCS3.06500889003
ens_2d_2BCX-RAY DIFFRACTIONTorsion NCS0.960819593314
ens_3d_2EEX-RAY DIFFRACTIONTorsion NCS0.745157911437
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.25-3.350.33021350.30652694X-RAY DIFFRACTION99.51
3.35-3.450.36831430.30112682X-RAY DIFFRACTION99.72
3.45-3.580.30431420.28652726X-RAY DIFFRACTION99.55
3.58-3.720.37451450.27722720X-RAY DIFFRACTION99.72
3.72-3.890.33881510.27042725X-RAY DIFFRACTION99.79
3.89-4.090.33711410.23912744X-RAY DIFFRACTION100
4.09-4.350.24411240.21342755X-RAY DIFFRACTION99.79
4.35-4.690.27471390.20262744X-RAY DIFFRACTION99.9
4.69-5.160.24071390.18942769X-RAY DIFFRACTION99.86
5.16-5.910.24941250.20592771X-RAY DIFFRACTION98.97
5.91-7.440.3011450.22532810X-RAY DIFFRACTION98.83
7.44-131.720.21731540.17322948X-RAY DIFFRACTION99.3
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.47638614222-1.12944522740.2629403140224.055007670780.5125051759023.424837400240.5106051209850.4866482955872.85690946801-0.266636284538-0.071311077072-0.447366955925-0.6594031880980.266114796523-0.2630796149591.22068294384-0.1318885989160.1745577763550.9543888536360.3077757754182.2503341959213.1570507812-23.18074019-6.24560772419
24.37960885195-0.8625692075150.6824563014573.93093855327-1.036860230772.973259074920.337207038360.3996305330280.042029607072-0.2372854335540.1749246149170.05590253240770.5499174590720.121019604064-0.5315963127770.8959694766780.120071823242-0.1771826387240.851290190815-0.1256282628940.75379414528410.8498130544-50.2617497824-2.47305097787
31.30029222479-2.271815197490.01019052222974.72380852346-3.198828005273.700495996690.4396881711510.127607766977-0.609357027817-0.785564083779-0.002045634523840.8587238427541.09069290606-0.224695489643-0.482911841391.509871656330.186851970161-0.5960685943520.94005467321-0.187987846951.1550682754519.746898926-84.484897670316.0673377508
46.13846163721-0.238932580997-5.102269291445.16918747056-0.2285814937856.527784734130.311661263152-0.147207201519-0.329827958984-0.753805654371-0.08335189865051.784432382890.0699060807108-0.796687438698-0.1149585125880.798513407346-0.00634620435228-0.3468426212310.871210562286-0.2569665375281.270300735114.6133277172-75.196370775938.8490458989
54.83611329502-2.63032154156-0.6561140047363.24210664082-1.33114354635.71362284764-0.1266452890590.277915879431-0.249351496707-0.80353057125-0.09408206995931.449455447981.18730684572-0.7272560139110.3751100829881.01056898108-0.14373180485-0.3334476035550.913066181199-0.1153476804721.6694835443911.0379096314-82.211410479540.1336042881
62.57689704373-0.3572455712430.7209869946414.210093791272.320884274114.321138206440.117124314388-0.287427718456-0.617351125057-0.349415422898-0.01956530996510.140530849745-0.4100877199720.0974749337835-0.1281591197230.5897296373140.0538816429712-0.03847043838641.01681202254-0.1853453591920.75857711288922.4609582099-65.37889820350.7936627809
75.369442870780.167777667889-1.184083261946.797403423990.04904833277912.93894328938-0.3179096186190.3925465128480.4446264155670.240100504030.2596565602360.0997377582989-0.105007790434-0.399055801063-0.04400883271350.578858560542-0.0969765987243-0.0161745238891.34130251952-0.2695330716970.67969295637225.6305918059-53.497239942860.8458067654
82.028991831040.226213950320.772306157475.35786964185-0.05934700064483.533776526160.285333223651-0.305118411187-0.3292907473320.1102159347340.06413965615180.2918209338460.695581965931.04269547725-0.3837245946650.9286508189470.341757330724-0.1678585323021.09031280489-0.179572840130.72881484745738.162437662-78.812243384235.811226542
92.57952772806-1.058175964720.2003869442685.556289640450.6637814722944.957456187260.655004339045-0.0417667001227-0.372443881161-1.22481476825-0.5989898760270.8724995549810.07647693421280.4835722530260.0129180361250.9400284711370.187864948498-0.2026227030090.962340621652-0.05967906184170.71795649400534.9763274297-73.321138136529.0963324754
103.138750596891.883044181010.3013234683149.303397814412.237662747084.305279757930.1252189205780.09434285018730.306678868816-1.74873629773-0.01584328482131.112159987520.3037844578720.536955698559-0.06156778153330.9860460232990.263050403605-0.2567331731850.848273094434-0.1763708110130.86245962049934.0888586446-73.680674241933.7418962786
117.875342484187.290517622854.489965933549.132614907354.476026586253.9089475840.879092548483-0.1798184788450.04043265951520.0658030791055-0.518380407476-0.2638137285140.5601102022190.0993990871057-0.4232710090870.902319803141-0.002712699791130.02116313943921.36991120259-0.3326134417970.80525412932338.1741725741-72.335117388943.5362718462
125.050289186430.9804610678962.465002781596.80526947903-0.3864132642627.901129303840.235866795444-0.635732920338-0.113333976737-0.009027963143290.389696831626-0.197092605534-0.0921862194165-0.53177036552-0.8370678737350.644279308689-0.0263397728816-0.04440979126081.32642525259-0.1682613425920.63173353975335.554210436-58.760826058966.5210045629
139.825251551034.130372478880.9211198165898.95440629493-5.127734616554.3957226905-0.0919274653764-1.28665185705-1.91087184770.500605648933-0.0940628035226-1.38751690179-1.79535044013-1.741514106850.5677831571160.7859067290550.0874632796862-0.00254880754741.15472558903-0.137268015880.71797180813342.2230217068-67.429120099774.4396219706
144.914206094831.84231899578-1.620474391224.98503487648-3.075132972398.51383623435-0.8389179696021.23016948174-0.465930173473-0.726343623661.08766729685-0.1284622123810.810929720024-1.26238292810.1469916203940.687093601601-0.1683015380820.04336644605531.38388234271-0.2134142315410.95240511347737.7269203479-62.790203948355.9662861122
158.26582920305-0.565977682831-0.6304519006982.71789930399-0.5718277935596.469475457920.0846726022135-1.81924286833-0.6529084167140.7533194843560.0103453539140.340464139907-0.2602599912240.638969792225-0.1889148058060.789928815607-0.01039387463560.08700967967521.65941050744-0.1522001774620.77492275300739.4289339316-61.382098199774.0892589896
165.594880780660.920025864951-0.4170182670514.739985077411.178145154172.392629445050.3918515433910.203244768881-0.481046977018-1.259354562-0.3594051221280.7142210479470.0737584053915-0.271754154415-0.02771821494491.028313225980.203905639499-0.2162936319590.804654801896-0.02859548180890.7492801896447.8896428522-107.73769472333.0574928469
177.770484666463.0775584522-1.022631188737.169950606543.91830310047.551346329990.0747510162779-0.684794400497-0.01504440175930.282827377060.0681418208284-0.1261101605180.0748831992026-0.342533184715-0.1956667527720.648378367942-0.0475036175281-0.04266783364131.0227345160.289681627350.73978367967860.5100189396-106.18518281664.5682539727
184.708940398051.37249944601-0.9372088147258.868175459131.443760154054.459609688960.434118950836-0.125413966960.0211634189042-0.945855004302-1.06935967307-0.00671681404228-0.03642131061650.7394396800210.4056461416991.056054792650.1233451980470.1197397661871.110398004090.2256323045940.87929066706474.9324848997-104.81966449932.1385308112
197.93126550676-3.70854195264-2.011607338482.459436356721.777432217681.851077207020.4667294632721.01299989513-1.27842881116-0.713136522161-0.618903221369-0.153473280058-0.2004553098710.6716850771220.5194026990811.318245658860.2713080511450.09461202796411.200957656840.1741898781591.0113496000569.0599520584-103.98345192724.5082457149
205.998224459840.540468198122.710966863435.0563914543-2.357139721354.928690804410.001203617849420.1285863144360.514589525381-0.830087580299-0.735264484547-1.14570877852-1.037402192110.3865473931041.200913829441.31768235410.1529636351280.1201527142310.7648439264360.1892776394970.80199257641964.6005715463-93.856695161730.6093406466
219.170712188141.938728400484.87284739144.48930638551-3.675617401598.340617494710.02665480755621.727205763780.203528967005-0.322041886756-0.855973668776-1.136815091570.1581783795292.388720407090.3811670805571.391293646180.4028879251230.2611058417541.316110498990.2671145374411.3261940123273.8026639752-99.50585643723.016465976
226.922511932143.085335171071.777638804015.18717734879-2.392575368515.680044160310.09050972961470.244635525816-0.396388547888-1.21303997153-0.206027673265-0.191441803417-0.5657976590951.041548310070.3375581720931.100160698030.2650346878730.08656457713950.9156353112880.01425431432190.62812834671467.4482897643-99.654515756330.3988132836
236.912857287632.176485283645.776146016412.757596155831.95734572295.34500172498-0.5615652037971.00338707916-0.241990619317-0.8471475355680.0746160266049-0.377518204507-0.6995856016811.82205056760.7205570723131.179493192410.06047715909170.07732369895461.203999450670.172145960040.82849593354371.7917842902-106.11927863137.8349538025
247.217759586924.7691102674-1.828692246157.90289931609-1.749573945257.983628923180.155409122035-1.136175859270.3902755300020.237458802997-0.1762372724950.6942803186531.272590746270.632633500695-0.2180142498040.8881624587010.09130196904660.08970620954611.537773924430.6798657930051.1346739125564.735660646-115.70806609870.4626602973
257.61044490264-2.07096946417-5.937222593072.385909288393.284828487878.858519438890.9536347016150.2777380223420.202943447274-0.6027938290920.429542708412-0.0650756972501-0.2511715205230.408211140612-1.726338518040.806306999925-0.0341078093848-0.01311152649311.110896104880.3998545080350.88160556741673.6839743537-111.66475689458.0991468568
262.19294954688-0.9307337695211.246165994325.164940777142.16630703435.33410749510.0259305596694-0.663931565625-2.616793567692.105921209390.856451114989-1.001513350881.393085612641.04282291547-0.8151228740011.288542521070.2581796607550.05378654390171.134853349560.2154994625751.209792269176.3431132515-125.06859236263.2091271845
275.352497129715.98684320189-2.228509501549.79019372625-2.881784131175.01990241617-0.6981037395120.7841063743011.32914533672-1.343035811271.669571251522.306387246920.190086169417-0.333202789267-1.208639850620.688531487689-0.0628961178848-0.1176808450141.252601369770.2953628536911.0741366968271.5968443657-109.02961401453.4763078971
289.91339094303-5.67623431206-1.96796652065.447320768025.122586326087.60975931294-0.442303455218-0.438777427967-3.004512320390.742173539828-0.967773848930.7567456647650.7349342652590.4585783614691.709441921120.657883363777-0.188538081373-0.00331943606651.315317608590.342196862741.274615048665.7086679077-123.70491385266.7954481096
292.15512000624-5.37309403568-3.155851108195.166064137874.979212395135.829060558310.200508501033-1.23922576757-0.28128689390.03014178759580.8436181001620.16917352511.58089816385-0.100263348776-1.360000792740.765369622909-0.192746309534-0.1080885029881.216211772040.4091950534551.1886491951377.0217642246-120.89568409865.6207285702
306.0695712442.8289313408-4.890912033688.96400476809-4.327343911254.193343851080.18400173702-0.555687804748-2.354245010911.31670672019-0.4862765739210.03774381716630.071004248269-0.505423210941-0.03082444105660.927625576103-0.0941109099871-0.1320704418791.763497805680.4729805658960.92280344988978.1274264851-117.49363845369.9997862089
311.259091240081.52456774053-0.7331382229483.11849477103-1.136017639681.536012763890.57770849904-0.9416696917521.58485027085-0.159097566483-0.1398639175311.0087201804-0.686817227560.468526389835-0.4973618840491.678395704140.1471636001080.2466071198931.57715022088-0.6373741243052.1604598883542.9572471993-30.340942589428.7306559423
324.49550705525-2.32960218707-0.6940366300496.6864163650.7320980098431.669908698581.554540892-1.171002190920.474435850714-2.06390030795-0.0962588640557-0.631985732627-1.828980269940.623763458623-1.423309357921.877420875620.04305833219270.3595366276491.26037955779-0.4715397707211.7064613532234.6869209714-26.243647921225.5404004078
334.61789548247-0.3392783580192.494078279249.613757165620.9809204057963.303474468511.76556053817-0.4865753760250.2938446623290.3788993079380.9192584728210.809962579587-0.5607344103410.24820475737-1.616521939851.68760548489-0.05199216161810.4209982166861.4971082943-0.3185364558731.7864151803231.5457209611-32.918912631530.8412237842
342.46614552069-1.79033118832-0.7718377967586.059388085360.2217804828380.5497716749890.086489841345-0.2829390504080.0552812552157-0.8816143554020.1653359892890.3754930087290.0600031409250.153934466082-0.2955659019641.548059028430.210775995664-0.1784205660061.10011123492-0.1091966898520.66885607258545.7957164707-71.77473302114.9376951129
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 27 through 178 )AA27 - 1781 - 143
22chain 'A' and (resid 179 through 259 )AA179 - 259144 - 224
33chain 'A' and (resid 260 through 329 )AA260 - 329225 - 294
44chain 'C' and (resid 1 through 57 )CB1 - 571 - 52
55chain 'C' and (resid 58 through 99 )CB58 - 9953 - 91
66chain 'C' and (resid 100 through 158 )CB100 - 15892 - 136
77chain 'C' and (resid 159 through 239 )CB159 - 239137 - 217
88chain 'G' and (resid 1 through 44 )GC1 - 441 - 38
99chain 'G' and (resid 45 through 91 )GC45 - 9139 - 75
1010chain 'G' and (resid 92 through 111 )GC92 - 11176 - 95
1111chain 'G' and (resid 112 through 131 )GC112 - 13196 - 116
1212chain 'G' and (resid 132 through 162 )GC132 - 162117 - 147
1313chain 'G' and (resid 163 through 173 )GC163 - 173148 - 158
1414chain 'G' and (resid 174 through 192 )GC174 - 192159 - 177
1515chain 'G' and (resid 193 through 232 )GC193 - 232178 - 215
1616chain 'D' and (resid 1 through 143 )DD1 - 1431 - 121
1717chain 'D' and (resid 144 through 239 )DD144 - 239122 - 217
1818chain 'E' and (resid 1 through 18 )EF1 - 181 - 18
1919chain 'E' and (resid 19 through 44 )EF19 - 4419 - 38
2020chain 'E' and (resid 45 through 75 )EF45 - 7539 - 61
2121chain 'E' and (resid 76 through 91 )EF76 - 9162 - 75
2222chain 'E' and (resid 92 through 111 )EF92 - 11176 - 95
2323chain 'E' and (resid 112 through 131 )EF112 - 13196 - 116
2424chain 'E' and (resid 132 through 146 )EF132 - 146117 - 131
2525chain 'E' and (resid 147 through 162 )EF147 - 162132 - 147
2626chain 'E' and (resid 163 through 173 )EF163 - 173148 - 158
2727chain 'E' and (resid 174 through 192 )EF174 - 192159 - 177
2828chain 'E' and (resid 193 through 206 )EF193 - 206178 - 191
2929chain 'E' and (resid 207 through 216 )EF207 - 216192 - 201
3030chain 'E' and (resid 217 through 232 )EF217 - 232202 - 215
3131chain 'B' and (resid 27 through 102 )BG27 - 1021 - 72
3232chain 'B' and (resid 103 through 134 )BG103 - 13473 - 100
3333chain 'B' and (resid 135 through 169 )BG135 - 169101 - 125
3434chain 'B' and (resid 170 through 329 )BG170 - 329126 - 284

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