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- PDB-8tct: Structure of 3K-GlcH bound Bacteroides thetaiotaomicron 3-Keto-be... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8tct | ||||||
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Title | Structure of 3K-GlcH bound Bacteroides thetaiotaomicron 3-Keto-beta-glucopyranoside-1,2-Lyase BT1 | ||||||
![]() | Sugar phosphate isomerase/epimerase | ||||||
![]() | ISOMERASE / Sugar Phosphate Isomerase | ||||||
Function / homology | Xylose isomerase-like, TIM barrel domain / Xylose isomerase-like TIM barrel / Xylose isomerase-like superfamily / isomerase activity / : / PHOSPHATE ION / : / Sugar phosphate isomerase/epimerase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lazarski, A.C. / Worrall, L.J. / Strynadka, N.C.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: An alternative broad-specificity pathway for glycan breakdown in bacteria. Authors: Nasseri, S.A. / Lazarski, A.C. / Lemmer, I.L. / Zhang, C.Y. / Brencher, E. / Chen, H.M. / Sim, L. / Panwar, D. / Betschart, L. / Worrall, L.J. / Brumer, H. / Strynadka, N.C.J. / Withers, S.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.5 KB | Display | ![]() |
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PDB format | ![]() | 55.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 991.9 KB | Display | ![]() |
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Full document | ![]() | 993.8 KB | Display | |
Data in XML | ![]() | 15.2 KB | Display | |
Data in CIF | ![]() | 22.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8tcdC ![]() 8tcrC ![]() 8tcsC ![]() 8tdaC ![]() 8tdeC ![]() 8tdfC ![]() 8tdhC ![]() 8tdiC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 33286.801 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: BT_2156 / Production host: ![]() ![]() | ||||
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#2: Chemical | ChemComp-UC6 / Mass: 162.141 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H10O5 / Feature type: SUBJECT OF INVESTIGATION | ||||
#3: Chemical | ChemComp-CO / | ||||
#4: Chemical | ChemComp-PO4 / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.46 Å3/Da / Density % sol: 72.41 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 27% PEG 300 0.11 M Phosphate Citrate pH 4.2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Feb 2, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1807 Å / Relative weight: 1 |
Reflection | Resolution: 1.86→45.69 Å / Num. obs: 49947 / % possible obs: 99.93 % / Redundancy: 19.9 % / CC1/2: 1 / Rmerge(I) obs: 0.081 / Rpim(I) all: 0.026 / Rrim(I) all: 0.085 / Net I/σ(I): 26.9 |
Reflection shell | Resolution: 1.86→1.93 Å / Redundancy: 20 % / Rmerge(I) obs: 2.902 / Num. unique obs: 4903 / CC1/2: 0.619 / Rpim(I) all: 0.951 / Rrim(I) all: 3.056 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.86→45.69 Å
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