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Yorodumi- PDB-8tcb: Structure of human C4b-binding protein alpha chain CCP domains 1 ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8tcb | ||||||
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Title | Structure of human C4b-binding protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M87 protein | ||||||
Components |
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Keywords | IMMUNE SYSTEM / immune evasion / human complement | ||||||
Function / homology | Function and homology information regulation of opsonization / response to symbiotic bacterium / negative regulation of complement activation, classical pathway / T cell mediated immunity / complement activation, classical pathway / Regulation of Complement cascade / positive regulation of protein catabolic process / blood microparticle / innate immune response / RNA binding ...regulation of opsonization / response to symbiotic bacterium / negative regulation of complement activation, classical pathway / T cell mediated immunity / complement activation, classical pathway / Regulation of Complement cascade / positive regulation of protein catabolic process / blood microparticle / innate immune response / RNA binding / extracellular space / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Streptococcus pyogenes (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.69 Å | ||||||
Authors | McGowan, M.A. / Kolesinski, P. / Ghosh, P. | ||||||
Funding support | United States, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2024 Title: Conservation of C4BP-binding sequence patterns in Streptococcus pyogenes M and Enn proteins. Authors: Kolesinski, P. / McGowan, M. / Botteaux, A. / Smeesters, P.R. / Ghosh, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8tcb.cif.gz | 182.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8tcb.ent.gz | 119.4 KB | Display | PDB format |
PDBx/mmJSON format | 8tcb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8tcb_validation.pdf.gz | 760.3 KB | Display | wwPDB validaton report |
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Full document | 8tcb_full_validation.pdf.gz | 763.6 KB | Display | |
Data in XML | 8tcb_validation.xml.gz | 16.9 KB | Display | |
Data in CIF | 8tcb_validation.cif.gz | 21.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tc/8tcb ftp://data.pdbj.org/pub/pdb/validation_reports/tc/8tcb | HTTPS FTP |
-Related structure data
Related structure data | 8tgtC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14163.894 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: C4BPA, C4BP / Production host: Escherichia coli (E. coli) / References: UniProt: P04003 #2: Protein | Mass: 12531.667 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pyogenes (bacteria) / Gene: emm / Production host: Escherichia coli (E. coli) / References: UniProt: Q6TLP8 #3: Chemical | ChemComp-K / | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | Has protein modification | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.84 Å3/Da / Density % sol: 56.7 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.1 M sodium citrate, pH 4.8, 0.2 M KH2PO4, 25% w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.98 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 5, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.69→68.81 Å / Num. obs: 17478 / % possible obs: 98.7 % / Redundancy: 6.8 % / Biso Wilson estimate: 54.92 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.09 / Rpim(I) all: 0.037 / Rrim(I) all: 0.097 / Net I/σ(I): 20.3 |
Reflection shell | Resolution: 2.69→2.82 Å / Redundancy: 3 % / Rmerge(I) obs: 0.953 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 2045 / CC1/2: 0.452 / Rpim(I) all: 0.641 / Rrim(I) all: 1.155 / % possible all: 90.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.69→68.81 Å / SU ML: 0.4636 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.1603 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.45 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.69→68.81 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION
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