+Open data
-Basic information
Entry | Database: PDB / ID: 8t5z | ||||||
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Title | Influenza PA-N Endonuclease (amino acids 52-74 truncation) | ||||||
Components | Polymerase acidic protein | ||||||
Keywords | VIRAL PROTEIN / HYDROLASE / Influenza endonuclease / resistance / drug discovery / metal-binding pharmacophore | ||||||
Function / homology | Function and homology information cap snatching / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / endonuclease activity / host cell cytoplasm / Hydrolases; Acting on ester bonds / viral RNA genome replication / DNA-templated transcription / host cell nucleus / RNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.03 Å | ||||||
Authors | Kohlbrand, A.J. / Cohen, S.M. | ||||||
Funding support | United States, 1items
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Citation | Journal: Biochemistry / Year: 2024 Title: Structural Studies of Inhibitors with Clinically Relevant Influenza Endonuclease Variants. Authors: Kohlbrand, A.J. / Stokes, R.W. / Sankaran, B. / Cohen, S.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8t5z.cif.gz | 101.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8t5z.ent.gz | 67.9 KB | Display | PDB format |
PDBx/mmJSON format | 8t5z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t5/8t5z ftp://data.pdbj.org/pub/pdb/validation_reports/t5/8t5z | HTTPS FTP |
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-Related structure data
Related structure data | 8t5wC 8t67C 8t6zC 8t81C 8t94C 8vgzC 8t5v C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 22424.547 Da / Num. of mol.: 1 / Fragment: N-terminal domain (UNP residues 1-198) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus / Strain: A/California/04/2009(H1N1) / Gene: PA / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: C3W5S0, Hydrolases; Acting on ester bonds | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49.05 % |
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Crystal grow | Temperature: 306 K / Method: vapor diffusion, hanging drop Details: 32% PEG (MW 4000 g/mol), 100 mM Tris (pH 8.35), and 220 mM sodium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Oct 8, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→65.7 Å / Num. obs: 25379 / % possible obs: 100 % / Redundancy: 20.2 % / Biso Wilson estimate: 44.41 Å2 / CC1/2: 1 / Net I/σ(I): 24 |
Reflection shell | Resolution: 2.03→2.1 Å / Num. unique obs: 1362 / CC1/2: 0.785 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.03→65.66 Å / SU ML: 0.3007 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.4762 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 54.98 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→65.66 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION / Auth asym-ID: A / Label asym-ID: A
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