S-ADENOSYLMETHIONINE / Extradiol ring-cleavage dioxygenase LigAB LigA subunit domain-containing protein / TP-methylase family protein Similarity search - Component
Mass: 18.015 Da / Num. of mol.: 488 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.26 Å3/Da / Density % sol: 45.59 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 240 mM sodium malonate pH 5-5.5, 8-12% PEG 3350 at a 1-1 or 2-1 ratio of protein to mother liquor. Protein concentration is 20mg/mL
-
Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N