[English] 日本語
![](img/lk-miru.gif)
- PDB-7ltc: Structure of the alpha-N-methyltransferase (SonM) and RiPP precur... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 7ltc | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA) heteromeric complex (no cofactor) | ||||||
![]() |
| ||||||
![]() | TRANSFERASE / posttranslational modifications / ribosomally synthesized and posttranslationally modified peptides / alpha-N-methyltransferase / borosin / SAM | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Miller, F.S. / Crone, K.K. / Jensen, M.R. / Shaw, S. / Harcombe, W.R. / Elias, M. / Freeman, M.F. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis. Authors: Miller, F.S. / Crone, K.K. / Jensen, M.R. / Shaw, S. / Harcombe, W.R. / Elias, M.H. / Freeman, M.F. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 152.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 118.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 7lteC ![]() 7ltfC ![]() 7lthC ![]() 7ltrC ![]() 7ltsC ![]() 5n0pS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 29068.326 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: SonM / Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 7850.653 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: SonA / Source: (gene. exp.) ![]() ![]() ![]() #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.91 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: Proteins were concentrated at 20 mg/mL and crystallized at pH ranging between 5.5-7 and using PEG 3,350 (0-20%) as precipitant PH range: 5.5-7 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 12, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033167 Å / Relative weight: 1 |
Reflection | Resolution: 2→47.18 Å / Num. obs: 43871 / % possible obs: 98.4 % / Redundancy: 4.5 % / CC1/2: 0.998 / Net I/σ(I): 15.53 |
Reflection shell | Resolution: 2→2.1 Å / Num. unique obs: 6015 / CC1/2: 0.988 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 5N0P Resolution: 2→47.18 Å / Cross valid method: THROUGHOUT
| ||||||||||||||||||||
Displacement parameters | Biso max: 135.76 Å2 / Biso mean: 30.3803 Å2 / Biso min: 12.25 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→47.18 Å
|