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- PDB-8t1i: Atomic model of the mammalian Mediator complex with MED26 subunit -

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Basic information

Entry
Database: PDB / ID: 8t1i
TitleAtomic model of the mammalian Mediator complex with MED26 subunit
Components
  • (Mediator of RNA polymerase II transcription subunit ...) x 26
  • Unknown Peptide
KeywordsGENE REGULATION / mouse mediator / complex / MED26 subunit
Function / homology
Function and homology information


positive regulation of T cell extravasation / Nuclear Receptor transcription pathway / negative regulation of smooth muscle cell differentiation / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / androgen biosynthetic process / positive regulation of G0 to G1 transition / retinal pigment epithelium development / mammary gland branching involved in thelarche ...positive regulation of T cell extravasation / Nuclear Receptor transcription pathway / negative regulation of smooth muscle cell differentiation / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / androgen biosynthetic process / positive regulation of G0 to G1 transition / retinal pigment epithelium development / mammary gland branching involved in thelarche / G0 to G1 transition / thyroid hormone receptor signaling pathway / Regulation of lipid metabolism by PPARalpha / regulation of vitamin D receptor signaling pathway / core mediator complex / Cytoprotection by HMOX1 / Estrogen-dependent gene expression / ventricular trabecula myocardium morphogenesis / thyroid hormone generation / nuclear retinoic acid receptor binding / mediator complex / positive regulation of keratinocyte differentiation / embryonic heart tube development / camera-type eye development / cellular response to thyroid hormone stimulus / positive regulation of hepatocyte proliferation / embryonic hindlimb morphogenesis / nuclear vitamin D receptor binding / peroxisome proliferator activated receptor binding / limb development / lens development in camera-type eye / positive regulation of intracellular estrogen receptor signaling pathway / embryonic hemopoiesis / nuclear thyroid hormone receptor binding / mammary gland epithelial cell proliferation / megakaryocyte development / histone acetyltransferase binding / cellular response to hepatocyte growth factor stimulus / cortical actin cytoskeleton / termination of RNA polymerase II transcription / LBD domain binding / fat cell differentiation / epithelial cell proliferation involved in mammary gland duct elongation / skeletal muscle cell differentiation / mammary gland branching involved in pregnancy / somatic stem cell population maintenance / monocyte differentiation / blastocyst development / general transcription initiation factor binding / negative regulation of neuron differentiation / hematopoietic stem cell differentiation / embryonic placenta development / negative regulation of keratinocyte proliferation / positive regulation of transcription initiation by RNA polymerase II / erythrocyte development / animal organ regeneration / nuclear retinoid X receptor binding / nuclear receptor-mediated steroid hormone signaling pathway / negative regulation of fibroblast proliferation / ubiquitin ligase complex / histone acetyltransferase activity / RNA polymerase II preinitiation complex assembly / keratinocyte differentiation / cellular response to epidermal growth factor stimulus / peroxisome proliferator activated receptor signaling pathway / lactation / positive regulation of erythrocyte differentiation / nuclear receptor coactivator activity / liver development / promoter-specific chromatin binding / nuclear receptor binding / nuclear estrogen receptor binding / protein-DNA complex / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / animal organ morphogenesis / mRNA transcription by RNA polymerase II / cell morphogenesis / brain development / chromatin DNA binding / transcription coactivator binding / protein import into nucleus / transcription corepressor activity / ubiquitin protein ligase activity / actin binding / heart development / angiogenesis / in utero embryonic development / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / transcription by RNA polymerase II / transcription coactivator activity / cytoskeleton / nuclear body / protein ubiquitination / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin binding / positive regulation of cell population proliferation / protein-containing complex binding / positive regulation of gene expression
Similarity search - Function
Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 ...Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med19, metazoa / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / : / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator complex subunit 16, central helical bridge / Mediator complex, subunit Med23 / Mediator complex subunit 23 / Mediator of RNA polymerase II transcription subunit 15 / : / : / ARC105/Med15 mediator subunit N-terminal KIX domain / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator complex subunit 30 / Mediator complex subunit 28 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / : / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / Mediator complex, subunit Med7 superfmaily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / TFIIS N-terminal domain profile. / TFIIS/LEDGF domain superfamily / Coactivator CBP, KIX domain superfamily / von Willebrand factor A-like domain superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 ...Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 20
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.68 Å
AuthorsZhao, H. / Asturias, F.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-67167 United States
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS) United States
CitationJournal: To Be Published
Title: An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II
Authors: Zhao, H. / Li, J. / Xiang, Y. / Malik, S. / Young, N. / Ryner, M. / Conte, A. / Roeder, R. / Shi, Y. / Casellas, R. / Asturias, F.
History
DepositionJun 2, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 12, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Mediator of RNA polymerase II transcription subunit 1
B: Mediator of RNA polymerase II transcription subunit 4
C: Mediator of RNA polymerase II transcription subunit 6
D: Mediator of RNA polymerase II transcription subunit 7
E: Mediator of RNA polymerase II transcription subunit 8
F: Mediator of RNA polymerase II transcription subunit 9
G: Mediator of RNA polymerase II transcription subunit 10
H: Mediator of RNA polymerase II transcription subunit 11
I: Mediator of RNA polymerase II transcription subunit 14
J: Mediator of RNA polymerase II transcription subunit 15
K: Mediator of RNA polymerase II transcription subunit 16
L: Mediator of RNA polymerase II transcription subunit 17
M: Mediator of RNA polymerase II transcription subunit 18
N: Mediator of RNA polymerase II transcription subunit 19
O: Mediator of RNA polymerase II transcription subunit 20
P: Mediator of RNA polymerase II transcription subunit 21
Q: Mediator of RNA polymerase II transcription subunit 22
R: Mediator of RNA polymerase II transcription subunit 23
S: Mediator of RNA polymerase II transcription subunit 24
T: Mediator of RNA polymerase II transcription subunit 25
U: Mediator of RNA polymerase II transcription subunit 26
V: Mediator of RNA polymerase II transcription subunit 27
W: Mediator of RNA polymerase II transcription subunit 28
X: Mediator of RNA polymerase II transcription subunit 29
Y: Mediator of RNA polymerase II transcription subunit 30
Z: Mediator of RNA polymerase II transcription subunit 31
a: Unknown Peptide


Theoretical massNumber of molelcules
Total (without water)1,372,76227
Polymers1,372,76227
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Mediator of RNA polymerase II transcription subunit ... , 26 types, 26 molecules ABCDEFGHIJKLMNOPQRSTUVWXYZ

#1: Protein Mediator of RNA polymerase II transcription subunit 1 / Mediator complex subunit 1 / Peroxisome proliferator-activated receptor-binding protein / PBP / ...Mediator complex subunit 1 / Peroxisome proliferator-activated receptor-binding protein / PBP / PPAR-binding protein / Thyroid hormone receptor-associated protein complex 220 kDa component / Trap220 / Thyroid receptor-interacting protein 2 / TR-interacting protein 2 / TRIP-2


Mass: 167354.859 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q925J9
#2: Protein Mediator of RNA polymerase II transcription subunit 4 / Mediator complex subunit 4


Mass: 29817.475 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CQA5
#3: Protein Mediator of RNA polymerase II transcription subunit 6 / Mediator complex subunit 6


Mass: 28473.553 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q921D4
#4: Protein Mediator of RNA polymerase II transcription subunit 7 / Cofactor required for Sp1 transcriptional activation subunit 9 / CRSP complex subunit 9 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 9 / CRSP complex subunit 9 / Mediator complex subunit 7


Mass: 27244.252 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CZB6
#5: Protein Mediator of RNA polymerase II transcription subunit 8 / Activator-recruited cofactor 32 kDa component / ARC32 / Mediator complex subunit 8


Mass: 29232.008 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D7W5
#6: Protein Mediator of RNA polymerase II transcription subunit 9 / Mediator complex subunit 9


Mass: 15729.063 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8VCS6
#7: Protein Mediator of RNA polymerase II transcription subunit 10 / Mediator complex subunit 10


Mass: 15683.943 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CXU0
#8: Protein Mediator of RNA polymerase II transcription subunit 11 / Mediator complex subunit 11


Mass: 13147.803 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D8C6
#9: Protein Mediator of RNA polymerase II transcription subunit 14 / Cofactor required for Sp1 transcriptional activation subunit 2 / CRSP complex subunit 2 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 2 / CRSP complex subunit 2 / Mediator complex subunit 14 / Thyroid hormone receptor-associated protein complex 170 kDa component / Trap170


Mass: 161144.766 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A2ABV5
#10: Protein Mediator of RNA polymerase II transcription subunit 15 / Mediator complex subunit 15 / Positive cofactor 2 glutamine/Q-rich-associated protein / PC2 ...Mediator complex subunit 15 / Positive cofactor 2 glutamine/Q-rich-associated protein / PC2 glutamine/Q-rich-associated protein / mPcqap


Mass: 86682.930 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q924H2
#11: Protein Mediator of RNA polymerase II transcription subunit 16 / Mediator complex subunit 16 / Thyroid hormone receptor-associated protein 5 / Thyroid hormone ...Mediator complex subunit 16 / Thyroid hormone receptor-associated protein 5 / Thyroid hormone receptor-associated protein complex 95 kDa component / Trap95 / Vitamin D3 receptor-interacting protein complex 92 kDa component / DRIP92


Mass: 91890.484 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q6PGF3
#12: Protein Mediator of RNA polymerase II transcription subunit 17 / Cofactor required for Sp1 transcriptional activation subunit 6 / CRSP complex subunit 6 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 6 / CRSP complex subunit 6 / Mediator complex subunit 17 / Thyroid hormone receptor-associated protein complex 80 kDa component


Mass: 72555.609 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8VCD5
#13: Protein Mediator of RNA polymerase II transcription subunit 18 / Mediator complex subunit 18


Mass: 23675.652 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CZ82
#14: Protein Mediator of RNA polymerase II transcription subunit 19 / Mediator complex subunit 19


Mass: 25866.531 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8C1S0
#15: Protein Mediator of RNA polymerase II transcription subunit 20 / Mediator complex subunit 20 / TRF-proximal protein homolog


Mass: 23213.775 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9R0X0
#16: Protein Mediator of RNA polymerase II transcription subunit 21 / Mediator complex subunit 21 / RNA polymerase II holoenzyme component SRB7 / RNAPII complex component SRB7


Mass: 15600.307 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CQ39
#17: Protein Mediator of RNA polymerase II transcription subunit 22 / Mediator complex subunit 22 / Surfeit locus protein 5 / Surf-5


Mass: 22304.750 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q62276
#18: Protein Mediator of RNA polymerase II transcription subunit 23 / Cofactor required for Sp1 transcriptional activation subunit 3 / CRSP complex subunit 3 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 3 / CRSP complex subunit 3 / Mediator complex subunit 23 / Protein sur-2 homolog / mSur-2


Mass: 156265.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q80YQ2
#19: Protein Mediator of RNA polymerase II transcription subunit 24 / Mediator complex subunit 24 / Thyroid hormone receptor-associated protein 4 / Thyroid hormone ...Mediator complex subunit 24 / Thyroid hormone receptor-associated protein 4 / Thyroid hormone receptor-associated protein complex 100 kDa component / Trap100 / mTRAP100


Mass: 110106.867 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q99K74
#20: Protein Mediator of RNA polymerase II transcription subunit 25 / Mediator complex subunit 25 / mMED25


Mass: 78173.328 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8VCB2
#21: Protein Mediator of RNA polymerase II transcription subunit 26 / Cofactor required for Sp1 transcriptional activation subunit 7 / CRSP complex subunit 7 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 7 / CRSP complex subunit 7 / Mediator complex subunit 26


Mass: 64773.477 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q7TN02
#22: Protein Mediator of RNA polymerase II transcription subunit 27 / Cofactor required for Sp1 transcriptional activation subunit 8 / CRSP complex subunit 8 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 8 / CRSP complex subunit 8 / Mediator complex subunit 27


Mass: 35343.605 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DB40
#23: Protein Mediator of RNA polymerase II transcription subunit 28 / Mediator complex subunit 28


Mass: 19558.145 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q920D3
#24: Protein Mediator of RNA polymerase II transcription subunit 29 / Intersex-like protein / Mediator complex subunit 29


Mass: 21027.727 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DB91
#25: Protein Mediator of RNA polymerase II transcription subunit 30 / Mediator complex subunit 30 / Thyroid hormone receptor-associated protein 6 / Thyroid hormone ...Mediator complex subunit 30 / Thyroid hormone receptor-associated protein 6 / Thyroid hormone receptor-associated protein complex 25 kDa component / Trap25


Mass: 20383.354 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CQI9
#26: Protein Mediator of RNA polymerase II transcription subunit 31 / Mediator complex subunit 31 / Mediator complex subunit SOH1


Mass: 15792.077 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9CXU1

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Protein/peptide , 1 types, 1 molecules a

#27: Protein/peptide Unknown Peptide


Mass: 1720.111 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Mouse mediator complex / Type: COMPLEX / Entity ID: all / Source: NATURAL
Molecular weightValue: 1.0 MDa / Experimental value: NO
Source (natural)Organism: Mus musculus (house mouse)
Buffer solutionpH: 7.9
SpecimenConc.: 0.1 mg/ml / Embedding applied: YES / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
EM embeddingMaterial: ice
VitrificationInstrument: HOMEMADE PLUNGER / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
MicroscopyModel: FEI TALOS ARCTICA
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 3500 nm / Nominal defocus min: 700 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm
Image recordingElectron dose: 22.5 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM softwareName: PHENIX / Version: dev_3758: / Category: model refinement
CTF correctionType: NONE
3D reconstructionResolution: 4.68 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 199078 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00850412
ELECTRON MICROSCOPYf_angle_d0.56369377
ELECTRON MICROSCOPYf_dihedral_angle_d12.5198086
ELECTRON MICROSCOPYf_chiral_restr0.0398561
ELECTRON MICROSCOPYf_plane_restr0.0039250

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