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- EMDB-40968: Atomic model of the mammalian Mediator complex with MED26 subunit -

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Basic information

Entry
Database: EMDB / ID: EMD-40968
TitleAtomic model of the mammalian Mediator complex with MED26 subunit
Map dataAtomic model of the mammalian Mediator complex with MED26 subunit
Sample
  • Complex: Mouse mediator complex
    • Protein or peptide: x 27 types
Keywordsmouse mediator / complex / GENE REGULATION / MED26 subunit
Function / homology
Function and homology information


epithelial cell proliferation involved in mammary gland duct elongation / positive regulation of T cell extravasation / mammary gland branching involved in thelarche / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / positive regulation of mediator complex assembly / positive regulation of G0 to G1 transition / negative regulation of smooth muscle cell differentiation / Nuclear Receptor transcription pathway / regulation of vitamin D receptor signaling pathway ...epithelial cell proliferation involved in mammary gland duct elongation / positive regulation of T cell extravasation / mammary gland branching involved in thelarche / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / positive regulation of mediator complex assembly / positive regulation of G0 to G1 transition / negative regulation of smooth muscle cell differentiation / Nuclear Receptor transcription pathway / regulation of vitamin D receptor signaling pathway / core mediator complex / blastocyst development / mammary gland branching involved in pregnancy / embryonic hindlimb morphogenesis / retinal pigment epithelium development / androgen biosynthetic process / regulation of RNA biosynthetic process / Regulation of lipid metabolism by PPARalpha / skeletal muscle cell differentiation / thyroid hormone generation / thyroid hormone receptor signaling pathway / Cytoprotection by HMOX1 / embryonic heart tube development / positive regulation of hepatocyte proliferation / Estrogen-dependent gene expression / camera-type eye development / mammary gland epithelial cell proliferation / lens development in camera-type eye / embryonic placenta development / positive regulation of keratinocyte differentiation / ventricular trabecula myocardium morphogenesis / megakaryocyte development / negative regulation of keratinocyte proliferation / mediator complex / nuclear retinoic acid receptor binding / positive regulation of intracellular estrogen receptor signaling pathway / cellular response to hepatocyte growth factor stimulus / embryonic hemopoiesis / cellular response to thyroid hormone stimulus / peroxisome proliferator activated receptor binding / nuclear vitamin D receptor binding / negative regulation of neuron differentiation / nuclear thyroid hormone receptor binding / stem cell population maintenance / cellular response to steroid hormone stimulus / cortical actin cytoskeleton / histone acetyltransferase activity / histone acetyltransferase binding / LBD domain binding / erythrocyte development / keratinocyte differentiation / animal organ regeneration / monocyte differentiation / nuclear receptor-mediated steroid hormone signaling pathway / limb development / positive regulation of transcription initiation by RNA polymerase II / general transcription initiation factor binding / negative regulation of fibroblast proliferation / fat cell differentiation / nuclear retinoid X receptor binding / lactation / ubiquitin ligase complex / RNA polymerase II preinitiation complex assembly / cell morphogenesis / peroxisome proliferator activated receptor signaling pathway / animal organ morphogenesis / positive regulation of erythrocyte differentiation / in utero embryonic development / liver development / cellular response to epidermal growth factor stimulus / protein import into nucleus / nuclear estrogen receptor binding / nuclear receptor binding / brain development / positive regulation of transcription elongation by RNA polymerase II / promoter-specific chromatin binding / protein-DNA complex / chromatin DNA binding / mRNA transcription by RNA polymerase II / transcription by RNA polymerase II / transcription coactivator binding / transcription coregulator activity / heart development / transcription corepressor activity / ubiquitin protein ligase activity / transcription regulator complex / angiogenesis / actin binding / DNA-binding transcription factor binding / RNA polymerase II-specific DNA-binding transcription factor binding / cytoskeleton / nuclear body / transcription coactivator activity / protein ubiquitination / RNA polymerase II cis-regulatory region sequence-specific DNA binding / positive regulation of gene expression / positive regulation of cell population proliferation / chromatin binding / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process
Similarity search - Function
Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 ...Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med19, metazoa / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / : / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator complex subunit 16, central helical bridge / Mediator of RNA polymerase II transcription subunit 15 / : / ARC105/Med15 mediator subunit N-terminal KIX domain / ARC105/Med15 mediator subunit central domain / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / : / : / : / : / : / : / Mediator complex subunit 30 / Mediator complex subunit 28 / Mediator of RNA polymerase II transcription subunit 14, RM2 / Mediator of RNA polymerase II transcription subunit 14, RM8 / Mediator of RNA polymerase II transcription subunit 14, RM6 / Med14 RM domain / Med14 RM domain / Med14 C-terminal domain / : / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med23 / Mediator complex subunit 23 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / : / Mediator complex subunit 16, N-terminal / : / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / : / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / TFIIS N-terminal domain profile. / TFIIS helical bundle-like domain / Transcription factor IIS, N-terminal / Coactivator CBP, KIX domain superfamily / TFIIS/LEDGF domain superfamily / von Willebrand factor A-like domain superfamily / WD domain, G-beta repeat
Similarity search - Domain/homology
Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 ...Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 26 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 19 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 20
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.68 Å
AuthorsZhao H / Asturias F
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-67167 United States
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS) United States
CitationJournal: Mol Cell / Year: 2024
Title: An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II.
Authors: Haiyan Zhao / Jiaqin Li / Yufei Xiang / Sohail Malik / Supriya V Vartak / Giovana M B Veronezi / Natalie Young / McKayla Riney / Jens Kalchschmidt / Andrea Conte / Seol Kyoung Jung / ...Authors: Haiyan Zhao / Jiaqin Li / Yufei Xiang / Sohail Malik / Supriya V Vartak / Giovana M B Veronezi / Natalie Young / McKayla Riney / Jens Kalchschmidt / Andrea Conte / Seol Kyoung Jung / Srinivas Ramachandran / Robert G Roeder / Yi Shi / Rafael Casellas / Francisco J Asturias /
Abstract: The essential Mediator (MED) coactivator complex plays a well-understood role in regulation of basal transcription in all eukaryotes, but the mechanism underlying its role in activator-dependent ...The essential Mediator (MED) coactivator complex plays a well-understood role in regulation of basal transcription in all eukaryotes, but the mechanism underlying its role in activator-dependent transcription remains unknown. We investigated modulation of metazoan MED interaction with RNA polymerase II (RNA Pol II) by antagonistic effects of the MED26 subunit and the CDK8 kinase module (CKM). Biochemical analysis of CKM-MED showed that the CKM blocks binding of the RNA Pol II carboxy-terminal domain (CTD), preventing RNA Pol II interaction. This restriction is eliminated by nuclear receptor (NR) binding to CKM-MED, which enables CTD binding in a MED26-dependent manner. Cryoelectron microscopy (cryo-EM) and crosslinking-mass spectrometry (XL-MS) revealed that the structural basis for modulation of CTD interaction with MED relates to a large intrinsically disordered region (IDR) in CKM subunit MED13 that blocks MED26 and CTD interaction with MED but is repositioned upon NR binding. Hence, NRs can control transcription initiation by priming CKM-MED for MED26-dependent RNA Pol II interaction.
History
DepositionJun 2, 2023-
Header (metadata) releaseJun 12, 2024-
Map releaseJun 12, 2024-
UpdateOct 16, 2024-
Current statusOct 16, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_40968.png

Downloads & links

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Map

FileDownload / File: emd_40968.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationAtomic model of the mammalian Mediator complex with MED26 subunit
Projections & slices

Image control

Size
Brightness
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AxesZ (Sec.)Y (Row.)X (Col.)
1.11 Å/pix.
x 560 pix.
= 621.6 Å		1.11 Å/pix.
x 560 pix.
= 621.6 Å		1.11 Å/pix.
x 560 pix.
= 621.6 Å

Surface

Projections

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.11 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.054
Minimum - Maximum-0.070911705 - 0.30787417
Average (Standard dev.)-0.00021929563 (±0.008145886)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions560560560
Spacing560560560
CellA=B=C: 621.60004 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half Map 1

Fileemd_40968_half_map_1.map
AnnotationHalf Map 1
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram (log scale)

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Half map: Half Map 2

Fileemd_40968_half_map_2.map
AnnotationHalf Map 2
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Sample components

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Entire : Mouse mediator complex

EntireName: Mouse mediator complex
Components
  • Complex: Mouse mediator complex
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 16
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 23
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 24
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 25
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 26
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 27
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 28
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 29
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 30
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
    • Protein or peptide: Unknown Peptide

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Supramolecule #1: Mouse mediator complex

SupramoleculeName: Mouse mediator complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 1.0 MDa

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Macromolecule #1: Mediator of RNA polymerase II transcription subunit 1

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 167.354859 KDa
SequenceString: MKAQGETEDS ERLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNG LGSHLSASGT ECYITSDMFY VEVQLDPAGQ LCDVKVAHHG ENPVSCPELV QQLREKNFEE FSKHLKGLVN L YNLPGDNK ...String:
MKAQGETEDS ERLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNG LGSHLSASGT ECYITSDMFY VEVQLDPAGQ LCDVKVAHHG ENPVSCPELV QQLREKNFEE FSKHLKGLVN L YNLPGDNK LKTKMYLALQ SLEQDLSKMA IMYWKATNAA PLDKILHGSV GYLTPRSGGH LMNMKYYASP SDLLDDKTAS PI ILHEKNV PRSLGMNASV TIEGTSAMYK LPIAPLIMGS HPADNKWTPS FSAVTSANSV DLPACFFLKF PQPIPVSKAF VQK LQNCTG IPLFETPPTY LPLYELITQF ELSKDPDPLP LNHNMRFYAA LPGQQHCYFL NKDAPLPDGQ SLQGTLVSKI TFQH PGRVP LILNMIRHQV AYNTLIGSCV KRTILKEDSP GLLQFEVCPL SESRFSVSFQ HPVNDSLVCV VMDVQDSTHV SCKLY KGLS DALICTDDFI AKVVQRCMSI PVTMRAIRRK AETIQADTPA LSLIAETVED MVKKNLPPAS SPGYGMTTGN NPMSGT TTP TNTFPGGPIT TLFNMSMSIK DRHESVGHGE DFSKVSQNPI LTSLLQITGN GGSTIGSSPT PPHHTPPPVS SMAGNTK NH PMLMNLLKDN PAQDFSTLYG SSPLERQNSS SGSPRMEMCS GSNKAKKKKS SRVPPDKPKH QTEDDFQREL FSMDVDSQ N PMFDVSMTAD ALDTPHITPA PSQCSTPPAT YPQPVSHPQP SIQRMVRLSS SDSIGPDVTD ILSDIAEEAS KLPSTSDDC PPIGTPVRDS SSSGHSQSAL FDSDVFQTNN NENPYTDPAD LIADAAGSPN SDSPTNHFFP DGVDFNPDLL NSQSQSGFGE EYFDESSQS GDNDDFKGFA SQALNTLGMP MLGGDNGEPK FKGSSQADTV DFSIISVAGK ALGAADLMEH HSGSQSPLLT T GELGKEKT QKRVKEGNGT GASSGSGPGS DSKPGKRSRT PSNDGKSKDK PPKRKKADTE GKSPSHSSSN RPFTPPTSTG GS KSPGSSG RSQTPPGVAT PPIPKITIQI PKGTVMVGKP SSHSQYTSSG SVSSSGSKSH HSHSSSSSSL ASASTSGKVK SSK SEGSSS SKLSGSMYAS QGSSGSSQSK NSSQTGGKPG SSPITKHGLS SGSSSTKMKP QGKPSSLMNP SISKPNISPS HSRP PGGSD KLASPMKPVP GTPPSSKAKS PISSGSSGSH VSGTSSSSGM KSSSGSASSG SVSQKTPPAS NSCTPSSSSF SSSGS SMSS SQNQHGSSKG KSPSRNKKPS LTAVIDKLKH GVVTSGPGGE DPIDSQMGAS TNSSNHPMSS KHNTSGGEFQ SKREKS DKD KSKVSASGGS VDSSKKTSES KNVGSTGVAK IIISKHDGGS PSIKAKVTLQ KPGESGGDGL RPQIASSKNY GSPLISG ST PKHERGSPSH SKSPAYTPQN VDSESESGSS IAERSYQNSP SSEDGIRPLP EYSTEKHKKH KKEKKKVRDK DRDKKKSH S MKPENWSKSP ISSDPTASVT NNPILSADRP SRLSPDFMIG EEDDDLMDVA LIGN

UniProtKB: Mediator of RNA polymerase II transcription subunit 1

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Macromolecule #2: Mediator of RNA polymerase II transcription subunit 4

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 29.817475 KDa
SequenceString: MAASSSGEKE KERMGGVSGM AGLGSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQLE EENQVLELLI HRDGDFQELM KLALNQGKV HHEMQALEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV ...String:
MAASSSGEKE KERMGGVSGM AGLGSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQLE EENQVLELLI HRDGDFQELM KLALNQGKV HHEMQALEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV PGDPRRPYPT DLEMRSGLLG QMNNPSTSGV NGHLPGDALA AGRLPDVLAP QYPWQSNDMS VNMLPPNHSS DF LLEPPGH NKENEDDVEV MSTDSSSSSS DSD

UniProtKB: Mediator of RNA polymerase II transcription subunit 4

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Macromolecule #3: Mediator of RNA polymerase II transcription subunit 6

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 28.473553 KDa
SequenceString: MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQEKVK P KAKRKEEP ...String:
MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQEKVK P KAKRKEEP SSIFQRQRVD ALLIDLRQKF PPRFVQQKSG EKPVPVDQAK KEAEPLPETV KSEEKESTKN IQQTVSTKGP PE KRMRLQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

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Macromolecule #4: Mediator of RNA polymerase II transcription subunit 7

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 27.244252 KDa
SequenceString: MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ...String:
MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ASLPDDLPHS EAGMRVKAEP MDTDDNSNCP GQNEQQRESS GHRRDQIIEK DAALCVLIDE MNERP

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

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Macromolecule #5: Mediator of RNA polymerase II transcription subunit 8

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 29.232008 KDa
SequenceString: MQREEKQLEA SLDALLNQVA DLKNSLGSFI YKLENEYDRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP ...String:
MQREEKQLEA SLDALLNQVA DLKNSLGSFI YKLENEYDRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP NKQTFNPGDT NALVAAVAFG KGLSNWRPSG SSGPGQPGQP GAGTILAGAS GLPQVQMPGA PNQQQPMLSG VQ MAQAGQP GKMPSGIKTN IKSASMHPYQ R

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

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Macromolecule #6: Mediator of RNA polymerase II transcription subunit 9

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 15.729063 KDa
SequenceString:
MASSGVAGGR QAEDTLQPPP ELLPESKPPP PPQPLPVAAL PPPAAPRPQS PAGAKEENYS FLPLVHNVIK CMDKDSPDLH QDLNALKTK FQELRKLIGT MPGIHVSPEQ QQQQLHSLRE QVRTKNELLQ KYKSLCMFEI PKD

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

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Macromolecule #7: Mediator of RNA polymerase II transcription subunit 10

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 10
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 15.683943 KDa
SequenceString:
MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLSQK LNFIVTGLQD IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERAL AKNEQVKGKI DTMKKFKSLL IQELSKVFPE DMAKYRSIRG EDHPPS

UniProtKB: Mediator of RNA polymerase II transcription subunit 10

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Macromolecule #8: Mediator of RNA polymerase II transcription subunit 11

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 11
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 13.147803 KDa
SequenceString:
MATYSLANER LRALEDIERE IGAILQNAGT AILELSKEKT NERLLDRQAA AFTTSVQHVE AELSAQIRYL TQVATGQPHE GSSYSSRKD CQMALKRVDY ARLKISDVAR TCEQMLEN

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

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Macromolecule #9: Mediator of RNA polymerase II transcription subunit 14

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 14
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 161.144766 KDa
SequenceString: MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKI EIVQFASRTR QLFVRLLALV KWANDAGKVE KCAMISSFLD QQAILFVDTA DRLASLARDA LVHARLPSFA I PYAIDVLT ...String:
MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKI EIVQFASRTR QLFVRLLALV KWANDAGKVE KCAMISSFLD QQAILFVDTA DRLASLARDA LVHARLPSFA I PYAIDVLT TGSYPRLPTC IRDKIIPPDP ITKIEKQATL HQLNQILRHR LVTTDLPPQL ANLTVANGRV KFRVEGEFEA TL TVMGDDP EVPWRLLKLE ILVEDKETGD GRALVHSMQI DFIHQLVQSR LFADEKPLQD MYNCLHCFCL SLQLEVLHSQ TLM LIRERW GDLVQVERYH AGKSLSLSVW NQQVLGRKTG TASVHKVTIK IDENDVSKPL QIFHDPPLPA SDSKLVERAM KIDH LSIEK LLIDSVHARA HQRLQELKAI LRSFNANESS SIETALPALI VPILEPCGNS ECLHIFVDLH SGMFQLMLYG LDPAT LEDM EKSLNDDMKR IIPWIQQLKF WLGQQRCKQS IKHLPTITTE TLQLANYSTH PIGSLSKNKL FIKLTRLPQY YIVVEM LEV PNKPTQLSYN YYFMSVSTAD REDSPVMALL LQQFKDNIQD LMSYTKTGKQ TRTGTKHKLS DDPCPIDSKK AKRSGEM CA FNKVLAHFVA MCDTNMPFVG LRLELSNLEI PHQGVQVEGD GFNHAIRLLK IPPCKGISEE TQKALDRSLL DCTFRLQG R NNRTWVAELV FANCPLNGTS TREQGPSRHV YLTYENLLSE PVGGRKVVEM FLNDWSSIAR LYECVLEFAR SLPEIPAHL NIFSEVRVYN YRKLILCYGT TKGSSISIQW NSIHQKFHIA LGTVGPNSGC SNCHNTILHQ LQEMFNKTPN VVQLLQVLFD TQAPLNAIN KLPTVPMLGL TQRTNTAYQC FSILPQSSTH IRLAFRNMYC IDIYCRSRGV VAIRDGAYSL FDNSKLVEGF Y PAPGLKTF LNMFVDSNQD ARRRSVNEDD NPPSPIGGDM MDSLISQLQP PQQQPFPKQP GTSGAYPLTS PPTSYHSTVN QS PSMMHTQ SPGNLHAASS PSGALRAPSP ASFVPTPPPS SHGISIGPGA SFASPHGTLD PSSPYTMVSP SGRAGNWPGS PQV SGPSPA TRLPGMSPAN PSLHSPVPDV SHSPRAGTSS QTMPTNMPPP RKLPQRSWAA SIPTILTHSA LNILLLPSPT PGLV PGLAG SYLCSPLERF LGSVIMRRHL QRIIQQETLQ LINSNEPGVI MFKTDALKCR VALSPKTNQT LQLKVTPENA GQWKP DELQ VLEKFFETRV AGPPFKANTL IAFTKLLGAP THILRDCVHI MKLELFPDQA TQLKWNVQFC LTIPPSAPPI APPGTP AVV LKSKMLFFLQ LTQKTSVPPQ EPVSIIVPII YDMASGTTQQ ADIPRQQNSS VAAPMMVSNI LKRFAEMNPP RQGECTI FA AVRDLMANLT LPPGGRP

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

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Macromolecule #10: Mediator of RNA polymerase II transcription subunit 15

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 15
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 86.68293 KDa
SequenceString: MDVSGQETDW RSAAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLT GGPTPGAAGI GMPPRGPGQS LGGMGGLGAM GQPLPLSGQP PPGTSGMAPH GMAVVSTATP QTQLQLQQVA L QQQQQQQQ ...String:
MDVSGQETDW RSAAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLT GGPTPGAAGI GMPPRGPGQS LGGMGGLGAM GQPLPLSGQP PPGTSGMAPH GMAVVSTATP QTQLQLQQVA L QQQQQQQQ QQQFQQQQAA LQQQQQQQQQ QQQQQQFQAQ QNAMQQQFQA VVQQQQLQQQ QQQQHLIKLH HQSQQQQIQQ QQ LQRMAQL QLQQQQQQQQ QQALQAQPPM QQPSMQQPQP PPSQALPQQL SQLHHPQHHQ PPPQAQQSPI AQNQPPQIPP QSQ SQPLVS QAQALPGPML YAAQQQLKFV RAPMVVQQPQ VQPQVQQVQP QVQPQAAVQA AQSAQMVAPG VQMIAEALAQ GGMH VRARF PPTSTMSAGP SSSISLGGQP TTQVSQSSLT MLSSPSPGQQ VQTPQSMPPP PQPSPQPGSQ PNSNVSSGPA PSPSS FLPS PSPQPSQSPV TARTPQNFSV PSPGPLNTPV NPSSVMSPAG SSQAEEQQYL DKLKQLSKYI EPLRRMINKI DKNEDR KKD LSKMKSLLDI LTDPSKRCPL KTLQKCEIAL EKLKNDMAVP TPPPPPVLPT KQQDLCQPLL DAVLANIRSP VFNHSLY RT FVPAMMAIHG PPIVSPVVCS RKRRFEEDER QSIPNVLQGE VARLDPKFLV NLDPSHCSNN GTVHLICKLD DKDLPSVP P LELSVPADYP AQSPMWIDRQ WQYDANPFLQ SVHRCMTSRL LQLPDKHSVT ALLNTWAQSI HQACLSAA

UniProtKB: Mediator of RNA polymerase II transcription subunit 15

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Macromolecule #11: Mediator of RNA polymerase II transcription subunit 16

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 16
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 91.890484 KDa
SequenceString: MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADA DGQIKCWSMA DHLANSWESS VGSQVEGDPI VALSWLHNGV KLALHVEKSG ASSFGEKFSR VKFSPSLTLF G GKPMEGWI ...String:
MDLAYVCEWE KWAKSTYCPS LPLACAWSCR NLIAFTTDLR NDDQDLTHMI HILDTEHPWE VHSVSSGHSE AITCLEWDQS GSRLLSADA DGQIKCWSMA DHLANSWESS VGSQVEGDPI VALSWLHNGV KLALHVEKSG ASSFGEKFSR VKFSPSLTLF G GKPMEGWI AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAA ADGSSASPVK FYKVCVSVVS EK CRIDTEI LPSLFMRCTT DPNRKDRFPA ITHLKFLARD MSEQVLLCAS SQTSSLVECW SLRKEGLPVN NIFQQISPVV GDK QPMILK WRILSATNDL DRVSAVALPK LPISLTNTDL KVASDTQFYP GLGLALAFQD GSVHMVHRLS LQTMAVFYSS APRS LDEPA LKRPRTTCPA VHFKAMQLSW TSLALVGIDN HGKLSMLRIS PSLGHPLEPK LALQHLLFLL EYCMVTGYDW WDILL HVQP GMVQSLVERL HEEYTRQKPA LQQVLSTRIL AMKASLCKLS PCTVARVCDY HTKLFLMAIT STLKSLLRPH FLNTPD KSP GDRLAEICAK ITDVDIDKVM INLKTEEFVL DMNTLQALQQ LLQWVGDFVL YLLVSLPNQG SPLRPGHSFL RDGTSLG ML RELMVVIRIW GLLKPSCLPV YTATSDTQDS MSLLFRLLTK LWICCRDEGA ASEPDEGLVD ECCLLPSQLL VPNLDWLP A SDGLVSRLQP KQPLRLRFGR APTLPSSTST LQLDGLTRAP GQPKIDHLRR LHLGAYPTEE CKACTRCGCV TMLKSPNKT TAVTQWEQRW IKNCLCGGLW RRVPLSCS

UniProtKB: Mediator of RNA polymerase II transcription subunit 16

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Macromolecule #12: Mediator of RNA polymerase II transcription subunit 17

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 17
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 72.555609 KDa
SequenceString: MSGVRAVRIS IESACEKQVQ EVGLDGTETY LQPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGPDGDAPDW GGAGADQDDE EGLVKFQPS LWPWDSVRNN LRSALTEMCV LYDVLSIVRD KKFMTLDPVS QDALPPKQSP QTLQLISKKK SLAGAAQILL K GAERLTKS ...String:
MSGVRAVRIS IESACEKQVQ EVGLDGTETY LQPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGPDGDAPDW GGAGADQDDE EGLVKFQPS LWPWDSVRNN LRSALTEMCV LYDVLSIVRD KKFMTLDPVS QDALPPKQSP QTLQLISKKK SLAGAAQILL K GAERLTKS VAENQENKLQ RDFNSELLRL RQHWKLRKVG DKILGDLSYR SAGSLFPHHG TFEVIKNTDI DLDKKIPEDY CP LDVQIPS DLEGSAYIKV SIQKQAPDIG DLGTVNLFKR PLPKSKPGSP HWQTKLEAAQ NVLLCKEIFA QLSREAVQIK SQI PHIVVK NQIISQPFPS LQLSISLCHS SDDKKSQKCA AEKPGQEDHL YVLEHNLHLL IREFHKQTLS SIVMPHPASA PFGH KRMRL SGPQAFDKNE INSIQSTEGL LEKIIKQAKH IFLRSRTAAT IDSLASRIED PQIQAHWSNI NDVYESSVKV LITSQ GYEQ ICKSIQLQLN IGVEQVRVVH RDGRVIMLSH QEQELQDFLL SQMSQHQVHA VQQLAKVMGW QVLSFSNHVG LGPIES IGN ASAITVASPS GDYAISVRNG PESGSKIMVQ FPRNQCKDLP KSDVLQDSKW SHLRGPFKEV QWNKMEGRNF VYKMELL MS ALSPCLL

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

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Macromolecule #13: Mediator of RNA polymerase II transcription subunit 18

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 18
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.675652 KDa
SequenceString: MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKVVVYKIF RILVPGNTDS T EALSLSYL ...String:
MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKVVVYKIF RILVPGNTDS T EALSLSYL VELSVVAPAG QDMVSDDMRN FAEQLKPLVH LEKIDPKRLM

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

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Macromolecule #14: Mediator of RNA polymerase II transcription subunit 19

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 19
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 25.866531 KDa
SequenceString: MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS APAGADKSTA GSGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILGG SFNPITGTML SGFRLHTGPL P EQCRLMHI ...String:
MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS APAGADKSTA GSGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILGG SFNPITGTML SGFRLHTGPL P EQCRLMHI QPPKKKNKHK HKQSRTQDPV PPETPSDSDH KKKKKKKEED PERKRKKKEK KKKKNRHSPD HPGMGSSQAS SS

UniProtKB: Mediator of RNA polymerase II transcription subunit 19

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Macromolecule #15: Mediator of RNA polymerase II transcription subunit 20

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 20
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 23.213775 KDa
SequenceString: MGVTCVSQMP VAEGKSLQQT VELLTKKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQAG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH ...String:
MGVTCVSQMP VAEGKSLQQT VELLTKKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQAG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH APGAPTVFGN RHDAVYGPAD TMIQYMELFN KIRKQQQVPV AGIR

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

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Macromolecule #16: Mediator of RNA polymerase II transcription subunit 21

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 21
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 15.600307 KDa
SequenceString:
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFSNIQT AINKDQPANP TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAAS LYKLEEENHE AATCLEDVVY RGDMLLEKIQ SALADIAQSQ LKTRSVTHSH SLPDS

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

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Macromolecule #17: Mediator of RNA polymerase II transcription subunit 22

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 22
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 22.30475 KDa
SequenceString: MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRALQ EECDRKLITL RDEVSIDLYE LEEEYYSSSS SLCEANDLPL CEAYWRLDLD A DSADGLSA ...String:
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRALQ EECDRKLITL RDEVSIDLYE LEEEYYSSSS SLCEANDLPL CEAYWRLDLD A DSADGLSA PLLASPETGA GPLQSAAPVH SHGGGPGPTE HT

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

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Macromolecule #18: Mediator of RNA polymerase II transcription subunit 23

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 23
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 156.265656 KDa
SequenceString: METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRMVCE SLINSDSLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK AILEKILTIP NTVSSAVVQQ L LAAREVIA ...String:
METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRMVCE SLINSDSLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK AILEKILTIP NTVSSAVVQQ L LAAREVIA YILERNACLL PAYFAVTEIR KLYPEGKLPH WLLGNLVSDF VDTFRPTARI NSICGRCSLL PVVNNSGAIC NS WKLDPAT LRFPLKGLLP YDKDLFEPQT ALLRYVLEQP YSRDMVCNML GLNKQHKQRC PVLEDQLVDL VVYAMERSET EEK FDDGGT SQLLWQHLSS QLIFFVLFQF ASFPHMVLSL HQKLAGRGLI KGRDHLMWVL LQFISGSIQK NALADFLPVM KLFD LLYPE KECIPVPDIN KPQSTHAFAM TCIWIHLNRK AQNGDSTLQI PIPHSLKLHH EFLQQSLRNK SLQMNDYKIA LLCNA YSTN SECFTLPMGA LVETIYGNGI MRVPLPGTSC LASASVTPLP MNLLDSLTVH AKMSLIHSIA TRVIKLAHTK SSVALA PAL VETYSRLLVY MEIESLGIKG FISQLLPTVF KSHAWGILHT LLEMFSHRMH HIQPHYRVQL LSHLHTLAAV AQTNQNQ LH LCVESTALRL ITALGSSEVQ PQFTRFLNDP KTVLSAESEE LNRALILTLA RATHVTDFFT GSDSIQGTWC KDILQTIM N FTPHNWASHT LSCFPAPLQA FFKQNNVPQE SRFNLKKNVE EEYRKWKSMT DENEIITQFS VQGFPPLFLC LLWKMLLET DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLL LLKPNDFRNR VSDFVKENSP EHWLQSDWHT KHMSYHKKYP EKLYFEGLAE QVDPPVPIQS PYLPIYFGNV C LRFLPVFD IVIHRFLELL PVSKSLETLL DHLGGLYKFH DRPVTYLYNT LHYYEMCLRN RDHLKRKLVH AIIGSLKDNR PQ GWCLSDT YLKHAMNARE DNPWVPEDSY YCKLIGRLVD TMAGKSPGPF PNCDWRFNEF PNPAAHALHV TCVELMALAV PGK DVGNAL LNVVLKSQPL VPRENITAWM NAIGLIITAL PEPYWIVLHD RIVNVISSSS LTSETEWVGY PFRLFDFTAC HQSY SEMSC SYTLALAHAV WHHSSIGQLS LIPKFLTEAL LPVVKTEFQL LYVYHLVGPF LQRFQQERTR CMIEIGVAFY DMLLN VDQC STHLNYMDPI CDFLYHMKYM FTGDSVKEQV EKIICNLKPA LKLRLRFITH ISKMEPAVPP QALNSGSPAP QSNQVP ASL PVTQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 23

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Macromolecule #19: Mediator of RNA polymerase II transcription subunit 24

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 24
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 110.106867 KDa
SequenceString: MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLAEALLEQA MIGPSPNPLI LSYLKYAISS QMVSCSSVLT AISKFDDFS RDLCVQALLD IMDMFCDRLS CHGKAEECIG LCRALLSALH WLLRCTAASA ERLQEGLEAG TPAPGEKQLA L CLQCLEKT ...String:
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLAEALLEQA MIGPSPNPLI LSYLKYAISS QMVSCSSVLT AISKFDDFS RDLCVQALLD IMDMFCDRLS CHGKAEECIG LCRALLSALH WLLRCTAASA ERLQEGLEAG TPAPGEKQLA L CLQCLEKT LSSTKNRALL HIAKLEEASS WTAIEHSLLK LGEILANLSN PQLRSQAERC GTLIRSIPSM LSVHSEQLHK TG FPTIHAL ILLEGTMNLT GEMQPLVEQL MMVKRMQHIP TPLFVLEIWK ACFVGLIESP EGTQELKWTA FTYLKIPQVL VKL KKYFHG EKDFTEDVNC AFEFLLKLTP LLDKADQRCN CDCTNFLLQE CNKQGLLSEV NFASLVGKRT ADRDPQLKSS ENAN IQPNP GLILRAEPTV TNILKTMDAD HSKSPEGLLG VLGHMLSGKS LDLLLAAAAA TGKLKSFARK FINLNEFTTH GSGES TKTA SVRALLFDIS FLMLCHVAQT YGSEVILSES SSGEEVPFFE TWMQTCMPEE GKILNPDHPC FRPDSTKVES LVALLN NSS EMKLVQMKWH EACLSISAAI LEILNAWENG VLAFESIQKI TDNIKGKVCS LAVCAVAWLV AHVRMLGLDE REKSLQM IR QLAGPLYSEN TLQFYNERVV IMNSILEHMC ADVLQQTATQ IKFPSTGVDT MPYWNLLPPK RPIKEVLTDI FAKVLEKG W VDSRSIHILD TLLHMGGVYW FCNNLIKELL KETRKEHTLR AVQLLYSIFC LDMQQVTLVL LGHILPGLLT DSSKWHSLM DPPGTALAKL AVWCALSSYS SHKGQASSRQ KKRHREDIED YVSLFPVEDM QPSKLMRLLS SSDDDANILS SPTDRSMNSS LSASQLHTV NMRDPLNRVL ANLFLLISSI LGSRTAGPHT QFVQWFMEEC VGCLEQDSRG SILQFMPFTT VSELVKVSAM S SPKVVLAI TDLSLPLGRQ VAAKAIAAL

UniProtKB: Mediator of RNA polymerase II transcription subunit 24

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Macromolecule #20: Mediator of RNA polymerase II transcription subunit 25

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 25
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 78.173328 KDa
SequenceString: MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTS SAYEFVTWLD GIKFMGGGGE SCSLIAEGLS TALQLFDDFK KMREQIGQTH RVCLLICNSP PYLLPAVEST T YSGCTTES ...String:
MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTS SAYEFVTWLD GIKFMGGGGE SCSLIAEGLS TALQLFDDFK KMREQIGQTH RVCLLICNSP PYLLPAVEST T YSGCTTES LVQKIGERGI HFSIVSPRKL PALRLLFEKA APPALLEPLQ QPADVSQDPR HMVLVRGLVL PVGGSSTSGS LQ TKQAVPL PPAPASAATL SAAPPQALPP VPPQYQVPGN LSAAQVAAQN AVEAAKSQKA GLGPRFSPIN PLQQAAPGVG PPF SQAPAP PLAPVPPGAP KPPPASQPSL VSTVAPGPVL AAPAQPGAPS LAGTVTPGGV NGPSAAQLGG PALGGQQSVS NKLL AWSGV LEWQEKPKPA SVDANTKLTR SLPCQVYVNH GENLKTEQWP QKLIMQLIPQ QLLTTLGPLF RNSRMVQFHF TNKDL ESLK GLYRIMGNGF AGCVHFPHTA PCEVRVLMLL YSSKKKIFMG LIPYDQSGFV NGIRQVITNH KQVQQQKLEQ QRGMGA QQA PPVLGPILEE QARPPQNLLQ LRAPQPQPQG AVGASAATGQ PQPQGATQAP TGAPQGPPGA APGPPPSGPI LRPQNPG AN PQLRSLLLNP APPQTGVPPP QASLHHLQPP GAPTLLPPHQ SMGQPQLGPQ LLHPPPAQSW PTQLPQRAPL PGQMLLSG G PRGPVPQPGL QPSVMEDDIL MDLI

UniProtKB: Mediator of RNA polymerase II transcription subunit 25

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Macromolecule #21: Mediator of RNA polymerase II transcription subunit 26

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 26
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 64.773477 KDa
SequenceString: MTAAPASPQQ MRDRLLQAID SQSNIRNMVA VLEVISSLER YPITKEALEE TRLGKLINDV RKKTKNEELA KRAKRLLRSW QKLIEPVHQ NEVALRALAG AAGSANGGAH NCRPEMGVAG APKSIHDLKN RNDIQRLPGQ RLDRLGSRKR RGDQRDLGHP G PPHKVSKG ...String:
MTAAPASPQQ MRDRLLQAID SQSNIRNMVA VLEVISSLER YPITKEALEE TRLGKLINDV RKKTKNEELA KRAKRLLRSW QKLIEPVHQ NEVALRALAG AAGSANGGAH NCRPEMGVAG APKSIHDLKN RNDIQRLPGQ RLDRLGSRKR RGDQRDLGHP G PPHKVSKG SPDPLVPNAS PLPTNGISGS PESLPSPLDG SGHLGPDGSR LEPSDNEKHS TKIPVNAVRP RPSSPGLGKP PV PCLQTKA AQLQQLDRAD ESPGPPYPRG SSRCSFSPRN SRHEGSFSRH RSSYIPKGQV SSPSPWPQPP DNTQVPSPLP LAQ PPTPPV RRQELLPNAE SPVHWPEQSE GHPRLTGPAC RAGFSPDSSK ADSDATSSGG SDSKKKKRYR PRDYTVNLDG QVAE AGVKP VRLKERKLTF DPMTRQIRPL TQKEPVRADS PVPTEQLPRT ELEQQEVKAS LQSPFEQTNW KELSRNEIIQ SYLSR QSSL LSSSGAQTPG AHHFMAEYLK QEESSRQGAR QPHVLLPLPT PTDLPGLTRE VTQDDLDRIQ AQQWPGVNGC EDTQGN WYD WTQCISLDPH GDDGRLNILP YVCLD

UniProtKB: Mediator of RNA polymerase II transcription subunit 26

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Macromolecule #22: Mediator of RNA polymerase II transcription subunit 27

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 27
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 35.343605 KDa
SequenceString: MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIANFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR ...String:
MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIANFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR IDRMFPEMSI HLSRPNGTSA MLLVTLGKVL KVIVVMRSLF IDRTIVKGYN ESVYTEDGKL DIWSKSSYQV FQ KVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 27

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Macromolecule #23: Mediator of RNA polymerase II transcription subunit 28

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 28
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 19.558145 KDa
SequenceString:
MAASLGGMFT GQPPGPPPPP PGLPGQASLL QAAPGAPRPS NSTLVDELES SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIAR QTECFFLQKR LQLSVQKPDQ VIKEDVSELR SELQRKDALV QKHLTKLRHW QQVLEDINVQ HKKPADMPQG S LAFLEQAS ANIPAPLKQT

UniProtKB: Mediator of RNA polymerase II transcription subunit 28

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Macromolecule #24: Mediator of RNA polymerase II transcription subunit 29

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 29
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 21.027727 KDa
SequenceString: MAAPQPQAAA VSSASGVSGP GSAGGPGPQQ QPQPTQLVGS AQSGLLQQQQ QDFDPVQRYK MLIPQLKESL QTLMKVAAQN LIQNTNIDN GQKSSDAPLQ RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV QPDSLPYPQY L AVIKAQIT ...String:
MAAPQPQAAA VSSASGVSGP GSAGGPGPQQ QPQPTQLVGS AQSGLLQQQQ QDFDPVQRYK MLIPQLKESL QTLMKVAAQN LIQNTNIDN GQKSSDAPLQ RFDKCLEEFY ALCDQLELCL RLAHECLSQS CDSAKHSPTL VPTATKPDAV QPDSLPYPQY L AVIKAQIT CAKDIHTALL DCANKVTGKT TAPSTGPGGS L

UniProtKB: Mediator of RNA polymerase II transcription subunit 29

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Macromolecule #25: Mediator of RNA polymerase II transcription subunit 30

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 30
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 20.383354 KDa
SequenceString:
MSTPPLAPTG MASGPFGGPQ AQQAAREVNT ATLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD HLRQLSILF RKLRLVYDKC NENCGGMDPI PVEQLIPYVD EDGSKNDDRA GPPRFASEER REIVEVNKKL KQKNQQLKQI M DQLRNLIW DINAMLAMRN

UniProtKB: Mediator of RNA polymerase II transcription subunit 30

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Macromolecule #26: Mediator of RNA polymerase II transcription subunit 31

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 31
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 15.792077 KDa
SequenceString:
MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKE PEYAKYLKYP QCLHMLELLQ YEHFRKELV NAQCAKFIDE QQILHWQHYS RKRVRLQQAL AEQQQQNNTA GK

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

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Macromolecule #27: Unknown Peptide

MacromoleculeName: Unknown Peptide / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 1.720111 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.1 mg/mL
BufferpH: 7.9
Sugar embeddingMaterial: ice
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: HOMEMADE PLUNGER

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 22.5 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.7000000000000001 µm
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.68 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 199078
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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