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- PDB-8sk6: human liver mitochondrial Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase -
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Open data
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Basic information
Entry | Database: PDB / ID: 8sk6 | ||||||
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Title | human liver mitochondrial Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase | ||||||
![]() | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial | ||||||
![]() | ISOMERASE / human / liver / mitochondrial / Delta(3 / 5)-Delta(2 / 4)-dienoyl-CoA isomerase | ||||||
Function / homology | ![]() delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity / Isomerases; Intramolecular oxidoreductases; Transposing C=C bonds / fatty acid beta-oxidation / peroxisomal matrix / Mitochondrial protein degradation / Peroxisomal protein import / peroxisome / mitochondrial matrix / mitochondrion / extracellular exosome ...delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity / Isomerases; Intramolecular oxidoreductases; Transposing C=C bonds / fatty acid beta-oxidation / peroxisomal matrix / Mitochondrial protein degradation / Peroxisomal protein import / peroxisome / mitochondrial matrix / mitochondrion / extracellular exosome / membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.96 Å | ||||||
![]() | Zhang, Z. / Tringides, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: High-Resolution Structural Proteomics of Mitochondria Using the 'Build and Retrieve' Methodology. Authors: Zhemin Zhang / Marios L Tringides / Christopher E Morgan / Masaru Miyagi / Jason A Mears / Charles L Hoppel / Edward W Yu / ![]() Abstract: The application of integrated systems biology to the field of structural biology is a promising new direction, although it is still in the infant stages of development. Here we report the use of ...The application of integrated systems biology to the field of structural biology is a promising new direction, although it is still in the infant stages of development. Here we report the use of single particle cryo-EM to identify multiple proteins from three enriched heterogeneous fractions prepared from human liver mitochondrial lysate. We simultaneously identify and solve high-resolution structures of nine essential mitochondrial enzymes with key metabolic functions, including fatty acid catabolism, reactive oxidative species clearance, and amino acid metabolism. Our methodology also identified multiple distinct members of the acyl-CoA dehydrogenase family. This work highlights the potential of cryo-EM to explore tissue proteomics at the atomic level. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 282.1 KB | Display | ![]() |
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PDB format | ![]() | 231 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 40556MC ![]() 8sgpC ![]() 8sgrC ![]() 8sgsC ![]() 8sgvC ![]() 8shsC ![]() 8sk8C ![]() 8skrC ![]() 8sksC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 35859.137 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: Q13011, Isomerases; Intramolecular oxidoreductases; Transposing C=C bonds |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase / Type: COMPLEX / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 105000 X / Nominal defocus max: 1500 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 35 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.20.1_4487: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.96 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 8862 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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