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Yorodumi- PDB-8rh8: CryoEM structure of human rho1 GABAA receptor in complex with GAB... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8rh8 | ||||||
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Title | CryoEM structure of human rho1 GABAA receptor in complex with GABA and estradiol in desensitized state | ||||||
Components | Gamma-aminobutyric acid receptor subunit rho-1 | ||||||
Keywords | MEMBRANE PROTEIN / Complex | ||||||
Function / homology | Function and homology information GABA receptor activation / GABA-A receptor activity / GABA-gated chloride ion channel activity / GABA-A receptor complex / gamma-aminobutyric acid signaling pathway / neurotransmitter receptor activity / chloride channel complex / transmembrane transporter complex / GABA-ergic synapse / chloride transmembrane transport ...GABA receptor activation / GABA-A receptor activity / GABA-gated chloride ion channel activity / GABA-A receptor complex / gamma-aminobutyric acid signaling pathway / neurotransmitter receptor activity / chloride channel complex / transmembrane transporter complex / GABA-ergic synapse / chloride transmembrane transport / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / modulation of chemical synaptic transmission / presynaptic membrane / chemical synaptic transmission / postsynaptic membrane / neuron projection / protein domain specific binding / glutamatergic synapse / synapse / protein-containing complex binding / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.66 Å | ||||||
Authors | Chen, F. / John, C. / Rebecca, J.H. / Erik, L. | ||||||
Funding support | 1items
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Citation | Journal: Nat Commun / Year: 2024 Title: Divergent mechanisms of steroid inhibition in the human rho1 GABAA receptor Authors: Chen, F. / John, C. / Rebecca, J.H. / Erik, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8rh8.cif.gz | 353.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8rh8.ent.gz | 287.1 KB | Display | PDB format |
PDBx/mmJSON format | 8rh8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8rh8_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 8rh8_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 8rh8_validation.xml.gz | 64 KB | Display | |
Data in CIF | 8rh8_validation.cif.gz | 87.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rh/8rh8 ftp://data.pdbj.org/pub/pdb/validation_reports/rh/8rh8 | HTTPS FTP |
-Related structure data
Related structure data | 19172MC 8rh4C 8rh7C 8rh9C 8rhgC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Protein / Sugars , 2 types, 15 molecules AEBCD
#1: Protein | Mass: 55950.230 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GABRR1 / Production host: Homo sapiens (human) / References: UniProt: P24046 #3: Sugar | ChemComp-NAG / |
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-Non-polymers , 5 types, 167 molecules
#2: Chemical | ChemComp-ABU / #4: Chemical | ChemComp-HEX / #5: Chemical | ChemComp-OCT / #6: Chemical | #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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Has protein modification | Y |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: human rho1 GABAA receptor in complex with estradiol / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 42 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 2.66 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 1401487 / Symmetry type: POINT | ||||||||||||||||||||||||
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