U2 snRNA 3'-end processing / regulation of fertilization / NELF complex / positive regulation of protein modification process / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / NTRK3 as a dependence receptor / negative regulation of DNA-templated transcription, elongation ...U2 snRNA 3'-end processing / regulation of fertilization / NELF complex / positive regulation of protein modification process / meiotic spindle elongation / Integration of energy metabolism / PP2A-mediated dephosphorylation of key metabolic factors / regulation of microtubule binding / NTRK3 as a dependence receptor / negative regulation of DNA-templated transcription, elongation / snRNA 3'-end processing / protein serine/threonine phosphatase complex / mitotic sister chromatid separation / MASTL Facilitates Mitotic Progression / regulation of meiotic cell cycle process involved in oocyte maturation / protein phosphatase type 2A complex / meiotic sister chromatid cohesion, centromeric / snRNA processing / peptidyl-serine dephosphorylation / flagellated sperm motility / peptidyl-threonine dephosphorylation / FAR/SIN/STRIPAK complex / DSIF complex / protein localization to nuclear envelope / positive regulation of microtubule binding / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / protein phosphatase regulator activity / protein antigen binding / GABA receptor binding / integrator complex / regulation of transcription elongation by RNA polymerase II / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Initiation of Nuclear Envelope (NE) Reformation / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / negative regulation of stem cell differentiation / ERKs are inactivated / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / 加水分解酵素; エステル加水分解酵素; 3'-リン酸モノエステル産生エンドリボヌクレアーゼ / negative regulation of epithelial to mesenchymal transition / positive regulation of DNA-templated transcription, elongation / Abortive elongation of HIV-1 transcript in the absence of Tat / regulation of growth / Disassembly of the destruction complex and recruitment of AXIN to the membrane / centrosome localization / inner cell mass cell proliferation / negative regulation of glycolytic process through fructose-6-phosphate / transcription elongation-coupled chromatin remodeling / positive regulation of NLRP3 inflammasome complex assembly / myosin phosphatase activity / CTLA4 inhibitory signaling / protein serine/threonine phosphatase activity / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / Platelet sensitization by LDL / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / protein-serine/threonine phosphatase / regulation of cell differentiation / RNA polymerase III activity / negative regulation of transcription elongation by RNA polymerase II / negative regulation of hippo signaling / ERK/MAPK targets / T cell homeostasis / regulation of G1/S transition of mitotic cell cycle / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / mesoderm development / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / phosphoprotein phosphatase activity 類似検索 - 分子機能
ジャーナル: Nature / 年: 2024 タイトル: Structural basis of Integrator-dependent RNA polymerase II termination. 著者: Isaac Fianu / Moritz Ochmann / James L Walshe / Olexandr Dybkov / Joseph Neos Cruz / Henning Urlaub / Patrick Cramer / 要旨: The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex ...The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex consisting of Pol II, the DRB sensitivity-inducing factor (DSIF) and the negative elongation factor (NELF) and how it cleaves the nascent RNA transcript, but has not explained how Integrator removes Pol II from the DNA template. Here we present three cryo-electron microscopy structures of the complete Integrator-PP2A complex in different functional states. The structure of the pre-termination complex reveals a previously unresolved, scorpion-tail-shaped INTS10-INTS13-INTS14-INTS15 module that may use its 'sting' to open the DSIF DNA clamp and facilitate termination. The structure of the post-termination complex shows that the previously unresolved subunit INTS3 and associated sensor of single-stranded DNA complex (SOSS) factors prevent Pol II rebinding to Integrator after termination. The structure of the free Integrator-PP2A complex in an inactive closed conformation reveals that INTS6 blocks the PP2A phosphatase active site. These results lead to a model for how Integrator terminates Pol II transcription in three steps that involve major rearrangements.
分子量: 24644.318 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: I3LSI7
#8: タンパク質
DNA-directedRNApolymeraseIIsubunitRPB7
分子量: 19314.283 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: A0A4X1VKG7
#10: タンパク質
DNA-directedRNApolymeraseIIsubunitRPB9 / RNA polymerase II subunit B9 / DNA-directed RNA polymerase II subunit I / RNA polymerase II 14.5 ...RNA polymerase II subunit B9 / DNA-directed RNA polymerase II subunit I / RNA polymerase II 14.5 kDa subunit / RPB14.5
分子量: 14541.221 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: P60899
#12: タンパク質
DNA-directedRNApolymeraseIIsubunitRPB11-a
分子量: 13310.284 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: F1RKE4
+
RNA polymerase II subunit ... , 2種, 2分子 DL
#5: タンパク質
RNApolymeraseIIsubunitD
分子量: 16331.255 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: A0A287ADR4
#13: タンパク質
RNApolymeraseIIsubunitK
分子量: 7018.244 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: A0A8D0JYF1
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DNA-directed RNA polymerases I, II, and III subunit ... , 3種, 3分子 FHJ
#7: タンパク質
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC2
分子量: 14477.001 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: A0A8D1KNW4
#9: タンパク質
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC3
分子量: 17162.273 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: I3LCB2
#11: タンパク質
DNA-directedRNApolymerasesI, II, andIIIsubunitRPABC5
分子量: 7655.123 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) Sus scrofa (ブタ) / 参照: UniProt: A0A4X1VYD0