+Open data
-Basic information
Entry | Database: PDB / ID: 8r7q | ||||||
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Title | human connexin-36 gap junction channel in complex with quinine | ||||||
Components | Gap junction delta-2 protein | ||||||
Keywords | MEMBRANE PROTEIN | ||||||
Function / homology | Function and homology information Electric Transmission Across Gap Junctions / connexin complex / Gap junction assembly / gap junction channel activity / neuronal action potential / visual perception / cell-cell signaling / chemical synaptic transmission / synapse / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.78 Å | ||||||
Authors | Ding, X.Y. / Blum, T.B. / Korkhov, V.M. | ||||||
Funding support | Switzerland, 1items
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Citation | Journal: Cell Discov / Year: 2024 Title: Structural basis of connexin-36 gap junction channel inhibition. Authors: Xinyue Ding / Simone Aureli / Anand Vaithia / Pia Lavriha / Dina Schuster / Basavraj Khanppnavar / Xiaodan Li / Thorsten B Blum / Paola Picotti / Francesco L Gervasio / Volodymyr M Korkhov / | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8r7q.cif.gz | 806.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8r7q.ent.gz | 680.3 KB | Display | PDB format |
PDBx/mmJSON format | 8r7q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r7/8r7q ftp://data.pdbj.org/pub/pdb/validation_reports/r7/8r7q | HTTPS FTP |
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-Related structure data
Related structure data | 18988MC 8qojC 8r7pC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 37078.766 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GJD2 / Production host: Homo sapiens (human) / References: UniProt: Q9UKL4 #2: Chemical | ChemComp-QI9 / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: human connexin-36 gap junction channel in complex with quinine Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 8 |
Specimen | Conc.: 2 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE / Humidity: 100 % |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
EM software | Name: PHENIX / Version: 1.20.1_4487 / Category: model refinement | ||||||||||||||||||||||||
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.78 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 231305 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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