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- PDB-8r5y: Structure of coxsackievirus B5 capsid (mutant CVB5F.cas.genogroup... -

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Basic information

Entry
Database: PDB / ID: 8r5y
TitleStructure of coxsackievirus B5 capsid (mutant CVB5F.cas.genogroupB) - A particle
ComponentsCoxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
KeywordsVIRUS / Enterovirus / coxsackievirus / thermostable / mutant
Biological speciesCoxsackievirus B5
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.7 Å
AuthorsKumar, K. / Antanasijevic, A.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Environ Sci Technol / Year: 2024
Title: Influence of Amino Acid Substitutions in Capsid Proteins of Coxsackievirus B5 on Free Chlorine and Thermal Inactivation.
Authors: Shotaro Torii / Jérôme Gouttenoire / Kiruthika Kumar / Aleksandar Antanasijevic / Tamar Kohn /
Abstract: The sensitivity of enteroviruses to disinfectants varies among genetically similar variants and coincides with amino acid changes in capsid proteins, although the effect of individual substitutions ...The sensitivity of enteroviruses to disinfectants varies among genetically similar variants and coincides with amino acid changes in capsid proteins, although the effect of individual substitutions remains unknown. Here, we employed reverse genetics to investigate how amino acid substitutions in coxsackievirus B5 (CVB5) capsid proteins affect the virus' sensitivity to free chlorine and heat treatment. Of ten amino acid changes observed in CVB5 variants with free chlorine resistance, none significantly reduced the chlorine sensitivity, indicating a minor role of the capsid composition in chlorine sensitivity of CVB5. Conversely, a subset of these amino acid changes located at the C-terminal region of viral protein 1 led to reduced heat sensitivity. Cryo-electron microscopy revealed that these changes affect the assembly of intermediate viral states (altered and empty particles), suggesting that the mechanism for reduced heat sensitivity could be related to improved molecular packing of CVB5, resulting in greater stability or altered dynamics of virus uncoating during infection.
History
DepositionNov 18, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 27, 2024Provider: repository / Type: Initial release
Revision 1.1Apr 3, 2024Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2Apr 10, 2024Group: Derived calculations / Category: pdbx_struct_assembly / pdbx_struct_oper_list
Item: _pdbx_struct_assembly.oligomeric_count / _pdbx_struct_assembly.oligomeric_details ..._pdbx_struct_assembly.oligomeric_count / _pdbx_struct_assembly.oligomeric_details / _pdbx_struct_oper_list.matrix[1][1] / _pdbx_struct_oper_list.matrix[1][2] / _pdbx_struct_oper_list.matrix[1][3] / _pdbx_struct_oper_list.matrix[2][1] / _pdbx_struct_oper_list.matrix[2][2] / _pdbx_struct_oper_list.matrix[2][3] / _pdbx_struct_oper_list.matrix[3][1] / _pdbx_struct_oper_list.matrix[3][2] / _pdbx_struct_oper_list.vector[1] / _pdbx_struct_oper_list.vector[2] / _pdbx_struct_oper_list.vector[3]

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
B: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
C: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1


Theoretical massNumber of molelcules
Total (without water)281,3863
Polymers281,3863
Non-polymers00
Water0
1
A: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
B: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
C: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
x 60


Theoretical massNumber of molelcules
Total (without water)16,883,165180
Polymers16,883,165180
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
point symmetry operation59

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Components

#1: Protein Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1


Mass: 93795.359 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Coxsackievirus B5 / Cell line (production host): BGMK / Production host: Homo sapiens (human)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Coxsackievirus B5 / Type: VIRUS / Details: Particle A state of the CVB5 virion capsid / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Coxsackievirus B5
Source (recombinant)Organism: Homo sapiens (human) / Cell: BGMK
Details of virusEmpty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION
Natural hostOrganism: Homo sapiens
Virus shellName: Capsid VP1-4 / Diameter: 300 nm
Buffer solutionpH: 7.5 / Details: TBS
Buffer component
IDConc.NameFormulaBuffer-ID
125 mMTris-HClTrisTris-HClTris1
2150 mMSodium chlorideNaClSodium chloride1
SpecimenConc.: 4.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Details: Inactivated by formaldehyde. Purified using a combination of sucrose gradient and size-exclusion chromatography.
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 283 K
Details: 3ul of sample applied. Blotting time varied. Blotting force = 0. Total blots = 1.

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Electron microscopy imaging

MicroscopyModel: TFS GLACIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 150000 X / Nominal defocus max: 1000 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: OTHER
Image recordingElectron dose: 40 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) / Num. of grids imaged: 2 / Num. of real images: 6653

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Processing

EM software
IDNameCategory
1cryoSPARCparticle selection
2EPUimage acquisition
4CTFFINDCTF correction
7UCSF Chimeramodel fitting
9cryoSPARCinitial Euler assignment
10cryoSPARCfinal Euler assignment
11cryoSPARCclassification
12cryoSPARC3D reconstruction
13Cootmodel refinement
14RosettaEMmodel refinement
Image processingDetails: EER files were imported to cryoSPARC live and motion correction was performed with Patch.
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 161820 / Details: Template picker used
SymmetryPoint symmetry: I (icosahedral)
3D reconstructionResolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 27505 / Algorithm: BACK PROJECTION / Details: Non-uniform refinement / Num. of class averages: 1 / Symmetry type: POINT
Atomic model buildingProtocol: RIGID BODY FIT / Space: REAL
Details: Initial fitting was performed in Chimera and model refinement was performed in Coot and Rosetta.
Atomic model buildingPDB-ID: 7XB2

7xb2


Accession code: 7XB2
Details: Asymmetric unit used for fitting and interpretation
Source name: PDB / Type: experimental model

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