+Open data
-Basic information
Entry | Database: PDB / ID: 8qko | ||||||
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Title | Connexin-43 gap junction channel in complex with mefloquine | ||||||
Components | Gap junction alpha-1 protein | ||||||
Keywords | MEMBRANE PROTEIN / connexin / gap junction channel / complex / mefloquine | ||||||
Function / homology | Function and homology information gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / milk ejection reflex / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / columnar/cuboidal epithelial cell maturation ...gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / milk ejection reflex / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / columnar/cuboidal epithelial cell maturation / glutathione transmembrane transporter activity / neuroblast migration / gap junction hemi-channel activity / negative regulation of trophoblast cell migration / microtubule-based transport / regulation of bone remodeling / monoatomic ion transmembrane transporter activity / SARS-CoV-2 targets PDZ proteins in cell-cell junction / regulation of ventricular cardiac muscle cell membrane depolarization / gap junction channel activity involved in cell communication by electrical coupling / contractile muscle fiber / Oligomerization of connexins into connexons / Transport of connexins along the secretory pathway / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / gap junction assembly / atrial cardiac muscle cell action potential / cellular response to pH / Regulation of gap junction activity / cardiac conduction system development / skeletal muscle tissue regeneration / regulation of atrial cardiac muscle cell membrane depolarization / connexin complex / Golgi-associated vesicle membrane / Formation of annular gap junctions / fascia adherens / Gap junction degradation / export across plasma membrane / cell communication by electrical coupling involved in cardiac conduction / cell-cell contact zone / gap junction / bone remodeling / Gap junction assembly / gap junction channel activity / adult heart development / regulation of bone mineralization / glutamate secretion / regulation of ventricular cardiac muscle cell membrane repolarization / tight junction / blood vessel morphogenesis / lens development in camera-type eye / xenobiotic transport / intermediate filament / embryonic digit morphogenesis / maintenance of blood-brain barrier / positive regulation of stem cell proliferation / RHOJ GTPase cycle / heart looping / efflux transmembrane transporter activity / beta-tubulin binding / RHOQ GTPase cycle / establishment of mitotic spindle orientation / intercalated disc / lateral plasma membrane / alpha-tubulin binding / T cell proliferation / positive regulation of vascular associated smooth muscle cell proliferation / bone development / tubulin binding / protein tyrosine kinase binding / neuron migration / negative regulation of cell growth / beta-catenin binding / osteoblast differentiation / male gonad development / monoatomic ion transmembrane transport / cellular response to amyloid-beta / protein localization / positive regulation of cold-induced thermogenesis / cell junction / cell-cell signaling / heart development / spermatogenesis / scaffold protein binding / positive regulation of canonical NF-kappaB signal transduction / in utero embryonic development / apical plasma membrane / membrane raft / Golgi membrane / negative regulation of gene expression / intracellular membrane-bounded organelle / signaling receptor binding / focal adhesion / endoplasmic reticulum membrane / positive regulation of gene expression / Golgi apparatus / signal transduction / mitochondrion / nucleoplasm / nucleus / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.73 Å | ||||||
Authors | Lavriha, P. / Korkhov, V.M. / Han, Y. | ||||||
Funding support | Switzerland, 1items
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Citation | Journal: To Be Published Title: Two distinct mechanisms of connexin channel inhibition by mefloquine and 2-aminoethoxydiphenyl borate Authors: Lavriha, P. / Han, Y. / Ding, X. / Qi, C. / Vaithia, A. / Korkhov, V.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8qko.cif.gz | 422.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8qko.ent.gz | 342.6 KB | Display | PDB format |
PDBx/mmJSON format | 8qko.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8qko_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 8qko_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 8qko_validation.xml.gz | 69.1 KB | Display | |
Data in CIF | 8qko_validation.cif.gz | 92.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qk/8qko ftp://data.pdbj.org/pub/pdb/validation_reports/qk/8qko | HTTPS FTP |
-Related structure data
Related structure data | 18468MC 8qjfC 8qjhC 8qk6C 8qkiC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 43061.336 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GJA1, GJAL / Production host: Homo sapiens (human) / References: UniProt: P17302 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Connexin-32 gap junction channel in complex with 2-aminoethoxydipheniyl borate Type: COMPLEX / Entity ID: all / Source: RECOMBINANT | |||||||||||||||
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Source (natural) | Organism: Homo sapiens (human) | |||||||||||||||
Source (recombinant) | Organism: Homo sapiens (human) | |||||||||||||||
Buffer solution | pH: 8 | |||||||||||||||
Buffer component |
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Specimen | Conc.: 5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | |||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 130000 X / Nominal defocus max: 2500 nm / Nominal defocus min: 500 nm / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.73 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 28940 / Symmetry type: POINT |