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Open data
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Basic information
Entry | Database: PDB / ID: 8qi1 | |||||||||
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Title | 360A Vipp1 H1-6 helical tubes | |||||||||
![]() | Membrane-associated protein Vipp1 | |||||||||
![]() | LIPID BINDING PROTEIN / membrane remodeling / membrane tubulation | |||||||||
Function / homology | PspA/IM30 / PspA/IM30 family / plasma membrane / Membrane-associated protein Vipp1![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 6.6 Å | |||||||||
![]() | Junglas, B. / Sachse, C. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for Vipp1 membrane binding: From rings and rods to carpets Authors: Junglas, B. / Kartte, D. / Ritter, I. / Schneider, D. / Sachse, C. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 41.8 KB | Display | ![]() |
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PDB format | ![]() | 24.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 24.9 KB | Display | |
Data in CIF | ![]() | 34.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 18430MC ![]() 8qfvC ![]() 8qhvC ![]() 8qhwC ![]() 8qhxC ![]() 8qhyC ![]() 8qhzC ![]() 8qi0C ![]() 8qi2C ![]() 8qi3C ![]() 8qi4C ![]() 8qi5C ![]() 8qi6C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 21060.562 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction |
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Sample preparation
Component | Name: Vipp1 H1-6 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: YES / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
EM embedding | Material: vitreous ice |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 48 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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Helical symmerty | Angular rotation/subunit: 84.9 ° / Axial rise/subunit: 1.76 Å / Axial symmetry: C1 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 6.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 145546 / Symmetry type: HELICAL | ||||||||||||||||||||||||
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