- PDB-8oee: Crystal structure of human AQP2 T126M mutant -
+
Open data
ID or keywords:
Loading...
-
Basic information
Entry
Database: PDB / ID: 8oee
Title
Crystal structure of human AQP2 T126M mutant
Components
Aquaporin-2
Keywords
MEMBRANE PROTEIN / Aquaporin / water channel
Function / homology
Function and homology information
cellular response to water deprivation / renal water transport / glycerol transmembrane transporter activity / Passive transport by Aquaporins / glycerol transmembrane transport / lumenal side of membrane / water transmembrane transporter activity / cellular response to mercury ion / water transport / water channel activity ...cellular response to water deprivation / renal water transport / glycerol transmembrane transporter activity / Passive transport by Aquaporins / glycerol transmembrane transport / lumenal side of membrane / water transmembrane transporter activity / cellular response to mercury ion / water transport / water channel activity / metanephric collecting duct development / renal water homeostasis / transport vesicle membrane / cellular response to copper ion / actin filament organization / recycling endosome / Vasopressin regulates renal water homeostasis via Aquaporins / basolateral plasma membrane / protein homotetramerization / apical plasma membrane / perinuclear region of cytoplasm / Golgi apparatus / extracellular exosome / membrane / plasma membrane Similarity search - Function
Aquaporin transporter / Major intrinsic protein, conserved site / MIP family signature. / Major intrinsic protein / Major intrinsic protein / Aquaporin-like Similarity search - Domain/homology
Aquaporin-2 / AQP-2 / ADH water channel / Aquaporin-CD / AQP-CD / Collecting duct water channel protein / WCH-CD ...AQP-2 / ADH water channel / Aquaporin-CD / AQP-CD / Collecting duct water channel protein / WCH-CD / Water channel protein for renal collecting duct
Mass: 25169.312 Da / Num. of mol.: 4 / Mutation: T126M Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: AQP2 / Production host: Komagataella pastoris (fungus) / References: UniProt: P41181
Mass: 18.015 Da / Num. of mol.: 15 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
N
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 3.18 Å3/Da / Density % sol: 61.3 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 20- 30% PEG400, Tris-HCl pH 8.5, 0.1M MgCl2, 0.1M NaCl, 0.025 M CdCl2 added to the drop only
-
Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source
Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 1.033 Å
Detector
Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 14, 2017
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.033 Å / Relative weight: 1
Reflection
Resolution: 3.15→89.98 Å / Num. obs: 42680 / % possible obs: 99.77 % / Redundancy: 11.3 % / Biso Wilson estimate: 99 Å2 / CC1/2: 0.998 / Net I/σ(I): 13.1