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- PDB-8jyr: Crystal structure of anti-HER2 antibody H2Mab-119 in complex with... -

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Basic information

Entry
Database: PDB / ID: 8jyr
TitleCrystal structure of anti-HER2 antibody H2Mab-119 in complex with HER2 domain I
Components
  • H2Mab-119 VH-CH1
  • H2Mab-119 light chain
  • Receptor tyrosine-protein kinase erbB-2
KeywordsIMMUNE SYSTEM / HER2 / antibody
Function / homology
Function and homology information


negative regulation of immature T cell proliferation in thymus / ERBB3:ERBB2 complex / ERBB2-ERBB4 signaling pathway / GRB7 events in ERBB2 signaling / immature T cell proliferation in thymus / RNA polymerase I core binding / semaphorin receptor complex / regulation of microtubule-based process / ErbB-3 class receptor binding / motor neuron axon guidance ...negative regulation of immature T cell proliferation in thymus / ERBB3:ERBB2 complex / ERBB2-ERBB4 signaling pathway / GRB7 events in ERBB2 signaling / immature T cell proliferation in thymus / RNA polymerase I core binding / semaphorin receptor complex / regulation of microtubule-based process / ErbB-3 class receptor binding / motor neuron axon guidance / Sema4D induced cell migration and growth-cone collapse / neurotransmitter receptor localization to postsynaptic specialization membrane / PLCG1 events in ERBB2 signaling / ERBB2-EGFR signaling pathway / neuromuscular junction development / ERBB2 Activates PTK6 Signaling / enzyme-linked receptor protein signaling pathway / positive regulation of Rho protein signal transduction / ERBB2-ERBB3 signaling pathway / Drug-mediated inhibition of ERBB2 signaling / Resistance of ERBB2 KD mutants to trastuzumab / Resistance of ERBB2 KD mutants to sapitinib / Resistance of ERBB2 KD mutants to tesevatinib / Resistance of ERBB2 KD mutants to neratinib / Resistance of ERBB2 KD mutants to osimertinib / Resistance of ERBB2 KD mutants to afatinib / Resistance of ERBB2 KD mutants to AEE788 / Resistance of ERBB2 KD mutants to lapatinib / Drug resistance in ERBB2 TMD/JMD mutants / positive regulation of transcription by RNA polymerase I / oligodendrocyte differentiation / ERBB2 Regulates Cell Motility / semaphorin-plexin signaling pathway / PI3K events in ERBB2 signaling / positive regulation of protein targeting to membrane / regulation of angiogenesis / Schwann cell development / regulation of ERK1 and ERK2 cascade / coreceptor activity / Signaling by ERBB2 / transmembrane receptor protein tyrosine kinase activity / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / myelination / positive regulation of MAP kinase activity / GRB2 events in ERBB2 signaling / positive regulation of cell adhesion / SHC1 events in ERBB2 signaling / cellular response to epidermal growth factor stimulus / Downregulation of ERBB2:ERBB3 signaling / Constitutive Signaling by Overexpressed ERBB2 / cell surface receptor protein tyrosine kinase signaling pathway / positive regulation of epithelial cell proliferation / basal plasma membrane / positive regulation of translation / phosphatidylinositol 3-kinase/protein kinase B signal transduction / wound healing / Signaling by ERBB2 TMD/JMD mutants / placental growth factor receptor activity / insulin receptor activity / vascular endothelial growth factor receptor activity / hepatocyte growth factor receptor activity / macrophage colony-stimulating factor receptor activity / platelet-derived growth factor alpha-receptor activity / platelet-derived growth factor beta-receptor activity / stem cell factor receptor activity / boss receptor activity / protein tyrosine kinase collagen receptor activity / brain-derived neurotrophic factor receptor activity / transmembrane-ephrin receptor activity / GPI-linked ephrin receptor activity / epidermal growth factor receptor activity / fibroblast growth factor receptor activity / insulin-like growth factor receptor activity / Signaling by ERBB2 ECD mutants / receptor protein-tyrosine kinase / neuromuscular junction / cellular response to growth factor stimulus / peptidyl-tyrosine phosphorylation / Signaling by ERBB2 KD Mutants / receptor tyrosine kinase binding / epidermal growth factor receptor signaling pathway / ruffle membrane / Downregulation of ERBB2 signaling / neuron differentiation / Constitutive Signaling by Aberrant PI3K in Cancer / transmembrane signaling receptor activity / PIP3 activates AKT signaling / myelin sheath / presynaptic membrane / heart development / RAF/MAP kinase cascade / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / protein tyrosine kinase activity / basolateral plasma membrane / early endosome / cell population proliferation / receptor complex / cell surface receptor signaling pathway / positive regulation of MAPK cascade
Similarity search - Function
: / Epidermal growth factor receptor transmembrane-juxtamembrane segment / Tyrosine protein kinase, EGF/ERB/XmrK receptor / Growth factor receptor domain 4 / Growth factor receptor domain IV / Receptor L-domain / Furin-like cysteine-rich domain / Receptor L-domain superfamily / Furin-like cysteine rich region / Receptor L domain ...: / Epidermal growth factor receptor transmembrane-juxtamembrane segment / Tyrosine protein kinase, EGF/ERB/XmrK receptor / Growth factor receptor domain 4 / Growth factor receptor domain IV / Receptor L-domain / Furin-like cysteine-rich domain / Receptor L-domain superfamily / Furin-like cysteine rich region / Receptor L domain / Furin-like repeat / Furin-like repeats / Growth factor receptor cysteine-rich domain superfamily / : / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Receptor tyrosine-protein kinase erbB-2
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.69 Å
AuthorsArimori, T. / Takagi, J.
Funding support Japan, 2items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS)21H02416 Japan
Japan Agency for Medical Research and Development (AMED)20am0101075 Japan
CitationJournal: Structure / Year: 2024
Title: Locally misfolded HER2 expressed on cancer cells is a promising target for development of cancer-specific antibodies.
Authors: Arimori, T. / Mihara, E. / Suzuki, H. / Ohishi, T. / Tanaka, T. / Kaneko, M.K. / Takagi, J. / Kato, Y.
History
DepositionJul 3, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Mar 13, 2024Provider: repository / Type: Initial release
Revision 1.1Mar 27, 2024Group: Database references / Category: citation / citation_author / Item: _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2May 15, 2024Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Oct 16, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Receptor tyrosine-protein kinase erbB-2
H: H2Mab-119 VH-CH1
L: H2Mab-119 light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)70,7694
Polymers70,5483
Non-polymers2211
Water8,557475
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)53.979, 112.291, 60.537
Angle α, β, γ (deg.)90.000, 90.761, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

#1: Protein Receptor tyrosine-protein kinase erbB-2 / Metastatic lymph node gene 19 protein / MLN 19 / Proto-oncogene Neu / Proto-oncogene c-ErbB-2 / ...Metastatic lymph node gene 19 protein / MLN 19 / Proto-oncogene Neu / Proto-oncogene c-ErbB-2 / Tyrosine kinase-type cell surface receptor HER2 / p185erbB2


Mass: 22726.814 Da / Num. of mol.: 1 / Fragment: domain I
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ERBB2, HER2, MLN19, NEU, NGL / Production host: Homo sapiens (human)
References: UniProt: P04626, receptor protein-tyrosine kinase
#2: Antibody H2Mab-119 VH-CH1


Mass: 23821.691 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Production host: Cricetulus griseus (Chinese hamster)
#3: Antibody H2Mab-119 light chain


Mass: 23999.420 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Production host: Cricetulus griseus (Chinese hamster)
#4: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 475 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.63 Å3/Da / Density % sol: 53.19 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.1 M Sodium citrate pH5.5, 20% w/v PEG3000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Dec 11, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9 Å / Relative weight: 1
ReflectionResolution: 1.69→48.65 Å / Num. obs: 79311 / % possible obs: 99.1 % / Redundancy: 3.52 % / Biso Wilson estimate: 26.84 Å2 / CC1/2: 0.997 / Rsym value: 0.066 / Net I/σ(I): 9.46
Reflection shellResolution: 1.69→1.8 Å / Redundancy: 3.6 % / Mean I/σ(I) obs: 1.35 / Num. unique obs: 12651 / CC1/2: 0.694 / Rsym value: 0.912 / % possible all: 97.9

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Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
XDSdata reduction
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.69→40.02 Å / SU ML: 0.2017 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.5072
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2087 3965 5 %
Rwork0.1734 75325 -
obs0.1752 79290 99.07 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 38.2 Å2
Refinement stepCycle: LAST / Resolution: 1.69→40.02 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4743 0 14 475 5232
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01464861
X-RAY DIFFRACTIONf_angle_d1.32416608
X-RAY DIFFRACTIONf_chiral_restr0.0907747
X-RAY DIFFRACTIONf_plane_restr0.0111851
X-RAY DIFFRACTIONf_dihedral_angle_d6.378664
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.69-1.710.36731290.35682457X-RAY DIFFRACTION91.44
1.71-1.740.35441440.33452734X-RAY DIFFRACTION99.62
1.74-1.760.32261410.31062668X-RAY DIFFRACTION99.57
1.76-1.780.34971430.29632722X-RAY DIFFRACTION99.65
1.78-1.810.3181410.27992679X-RAY DIFFRACTION99.82
1.81-1.830.35571430.28312720X-RAY DIFFRACTION99.79
1.83-1.860.32941430.26142707X-RAY DIFFRACTION99.89
1.86-1.890.28321420.24172695X-RAY DIFFRACTION99.89
1.89-1.930.26841420.21692700X-RAY DIFFRACTION99.68
1.93-1.960.21221420.19182696X-RAY DIFFRACTION99.82
1.96-20.26431430.19632709X-RAY DIFFRACTION99.55
2-2.040.2271410.18782682X-RAY DIFFRACTION99.65
2.04-2.080.23931420.18892702X-RAY DIFFRACTION99.23
2.08-2.130.23491410.17692682X-RAY DIFFRACTION99.54
2.13-2.190.231420.17612699X-RAY DIFFRACTION99.34
2.19-2.250.22661420.17772695X-RAY DIFFRACTION98.99
2.25-2.310.21981410.16632668X-RAY DIFFRACTION98.8
2.31-2.390.20091410.17332689X-RAY DIFFRACTION99.19
2.39-2.470.23811420.17832698X-RAY DIFFRACTION99.47
2.47-2.570.22951420.18212693X-RAY DIFFRACTION99.26
2.57-2.690.24011420.18412704X-RAY DIFFRACTION99.41
2.69-2.830.20941430.18372715X-RAY DIFFRACTION99.31
2.83-3.010.22471420.19022699X-RAY DIFFRACTION99.27
3.01-3.240.22121410.18382676X-RAY DIFFRACTION99.02
3.24-3.560.21391420.16792700X-RAY DIFFRACTION98.51
3.56-4.080.16631420.14132694X-RAY DIFFRACTION98.78
4.08-5.140.12411420.11692702X-RAY DIFFRACTION98.78
5.14-40.020.18011440.14742740X-RAY DIFFRACTION98.7
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.46846502152.29462601876-0.405811716795.76664115369-1.022559210091.21469501767-0.1155305469640.270772186662-0.406072918771-0.03858450933330.170905443019-0.694794817905-0.3430535183870.362877545116-0.1490841948150.236351735969-0.03968101881770.0262197624020.26632224384-0.0940805890020.3700184497921.833290704139.5714484247-18.425848958
22.853535089930.02739654318020.4178661480215.510700395510.6082676612732.12365540566-0.02883978849570.0487935426971-0.1315405988160.1055680199550.149414984659-0.356463038951-0.1266822265540.121048365467-0.1213440726650.201524124717-0.009601653068480.009002714543420.218382195835-0.02476323912750.21967365297111.75902735433.3108445133-16.4439109027
35.4073576548-0.897527611691-0.1024611403478.007537246170.7518036105515.04265923779-0.2899568289970.444330866193-0.369132831943-0.3740767720260.315903514513-0.223434202436-0.1413455856280.0203205446987-0.02281530127850.244938725202-0.0687783711870.04332852309070.217869861254-0.05724302044150.18755198394911.103614030631.5701398596-24.6148189797
43.064108128861.89457456362-0.229250612172.799551280370.307380300140.367903895922-0.165814269080.245145862169-0.0972465088112-0.391711146650.228498519575-0.263988416981-0.03616053897090.12084337108-0.07285859296540.250057790806-0.0300286554780.009442135477070.211870733569-0.02838371321840.19543045385715.642182158139.9461265888-18.6260207984
53.442436464661.163152346960.03131337623677.602569290955.03526017889.23092170679-0.118527057125-0.5643758270060.1990138213510.009463294633710.141988058142-0.40107564934-0.3731264534530.3385822944870.07305550055750.1946401816890.0556116765617-0.04256259514130.349573840105-0.03821749625040.31508366551933.130378294467.111950043-6.45385685672
65.175282446280.2617633013860.2987619675544.131888851681.247540956972.90219761988-0.244697971257-0.530297220791-0.3628414229110.2956517268310.158866598737-0.2507393995550.394523263885-0.04190775069180.1006846106190.2911520286030.0246794283230.04195565252980.2849260116870.03508328563080.30256289165929.284326436556.3272317664-7.19032394641
75.979602592021.516740811390.6021888835716.1474203423.140256997221.63139004715-0.3275233569620.02564146180690.00765567505386-0.712182650540.19411387041-0.0817681454497-0.736094428686-0.5635837872710.1745500655530.187231019160.01420096017020.003572352002260.2074393248960.02725066641920.24194667194527.28962686861.9933758123-13.764622207
84.623456878871.477162381610.8450679823395.349792502943.774039281436.95063263292-0.186364014028-0.5893905494160.04543287830430.2554821813950.127292885708-0.3769831804030.1532147517540.5135157931870.3766488436080.2675451634120.0562687094402-0.03935176313910.405606972188-0.03280491800050.45489309200538.825779296161.2942735751-5.19198357096
95.088699104184.691585552885.010219716777.165930741355.589267314.933019580450.0122279337634-0.04485220058540.20912475084-0.169293369647-0.09763115647290.438818376648-0.256909504442-0.2038622505030.1198176794530.274168181110.0233869772716-0.004560112692290.189543761920.008938140576040.292338978604-3.6524196635852.4344666206-6.24396826985
102.711769003890.828998521674-0.04420290669463.288241560370.8284309344313.116298456230.07563473612-0.1244946807740.1158970354470.0725425471377-0.08502830297970.12346043117-0.00156597549263-0.05635550215330.00968929648210.2092566666540.01371600922220.00511222303260.1726919217580.006284181251690.204920782052-0.01627172439740.5031614691-7.95334345609
112.507686650510.6746780318080.3964587936324.642471167331.432117668544.575843812560.0575356121237-0.192767053747-0.03291639531410.32966128315-0.03014494898520.1377413736760.0456420618948-0.2048533060550.05287990113830.242232121929-0.0206950737020.04361764297790.195367428958-0.00463560303840.231826787106-3.1738104329242.0714248371-0.379544024638
120.5183599406331.261343833160.4649465988733.602578388041.86788698451.08468692069-0.0556181356282-0.03535106162440.0344939892769-0.1370381536210.003537456798920.14089341352-0.0470857937929-0.001770692669040.03328694314770.2343208446410.015106381875-0.009463116914270.2634148795820.001646338123630.2672182922823.5677378004650.7478653128-5.75277254232
136.589101053940.819081604549-4.316800243880.883930150239-0.6732014989884.134551689820.01064113852940.1566641495990.407402508143-0.1124316441670.205656880691-0.0332526892983-0.112131875319-0.0892523858421-0.2288462327560.239383831163-0.007597882309540.000676718539690.243393576763-0.0137834460580.29461210358725.760436711373.8964599947-10.9328907459
147.683117773764.90253626294-5.672766260525.98616207131-3.237635073124.21098541846-0.4142668639140.4496482310140.0913720999211-0.3886064055840.3987537535650.06116943805330.346767228883-0.4184634295070.02477135001410.226780563529-0.0152522623748-0.03583420517680.304349180262-0.01391596691860.25210692546517.104257230464.7686068869-7.71812748383
154.526081043410.693742403028-2.194289271072.96105803213-0.2700502011351.895982153460.2928574693620.2678200222410.765297213344-0.4312295266820.152412379745-0.283479833793-0.400206754695-0.00191158682957-0.4193084061410.348959740323-0.001099170236470.1016784420590.2764672531910.01641519420940.43608576058126.562519136679.0948740899-11.8629701187
162.895655791540.0222399270338-0.3020747704743.0748056596-0.1819836129192.74541175670.01773171069620.070547922078-0.274411585253-0.0436563862179-0.0009687843274210.1511486012060.468071639689-0.251238613543-0.004075359443510.314315162584-0.0596275945487-0.03705136910070.255484771586-0.006538896351610.228447597997-5.958844316692.76488846204-18.5020508927
176.630211158891.152579868320.1690157425167.06825749271.804291432075.702998741020.03285293967010.08077738613750.02424213190760.04540241878110.0502213540660.1433137409750.230447250914-0.253588395436-0.01163537448750.201237644655-0.02243966701870.004032918932250.178897599640.05402305397390.154904284535-5.0317310936714.0157952854-20.5535277042
186.325089805834.057989556514.285564454062.624177111072.680959189762.98629968045-0.2410354738010.488934053309-0.288580348076-0.5257854723480.0565197394342-0.3012913878870.9677710128270.630818849366-0.06284299431350.577025970725-0.03129712200080.1086731200830.34887966044-0.04656000851340.4135342324914.991969474843.46630702201-32.1206118474
191.97242218998-0.761184396551-1.31656599212.703036155371.576116641082.71005460442-0.02117875346850.162592195291-0.0304485030252-0.3301571388110.05448797682580.0558189766938-0.0977322657674-0.0990415600047-0.03836960788370.235694874857-0.0297135939393-0.03104994162760.2440424793350.0177815624730.225689881534-2.7918908908219.1900899996-27.355448617
203.49265129052-3.226804722622.844926626733.13446676905-2.427988138892.833738490850.3506282805670.640638230811-0.516355816518-0.639552455196-0.1105586240460.3066883180690.0510922190493-0.278888012519-0.1118718474530.4445930958580.00217528152994-0.02360686024520.454093209649-0.05810466463980.2746698155210.78164619224613.7349457903-41.1955088955
212.188006310971.306738667471.694067996292.08835013641.464681367045.249691115030.1695095846560.6387337695920.122516399234-0.385271511911-0.2010675836610.835349574988-0.0248390196905-0.5476709633320.05393437218510.502932852382-0.0629172856534-0.04445689719760.4874886482740.02112309123240.407828767246-9.0945943692417.2195351494-38.4638277478
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'H' and (resid 1 through 17 )HC1 - 171 - 17
22chain 'H' and (resid 18 through 56 )HC18 - 5618 - 57
33chain 'H' and (resid 57 through 75 )HC57 - 7558 - 76
44chain 'H' and (resid 76 through 119 )HC76 - 11977 - 126
55chain 'H' and (resid 120 through 145 )HC120 - 145127 - 148
66chain 'H' and (resid 146 through 168 )HC146 - 168149 - 171
77chain 'H' and (resid 169 through 184 )HC169 - 184172 - 187
88chain 'H' and (resid 185 through 214 )HC185 - 214188 - 217
99chain 'L' and (resid 1 through 25 )LD1 - 251 - 25
1010chain 'L' and (resid 26 through 48 )LD26 - 4826 - 52
1111chain 'L' and (resid 49 through 75 )LD49 - 7553 - 79
1212chain 'L' and (resid 76 through 113 )LD76 - 11380 - 117
1313chain 'L' and (resid 114 through 155 )LD114 - 155118 - 159
1414chain 'L' and (resid 156 through 174 )LD156 - 174160 - 178
1515chain 'L' and (resid 175 through 211 )LD175 - 211179 - 215
1616chain 'A' and (resid 23 through 92 )AA23 - 921 - 70
1717chain 'A' and (resid 93 through 117 )AA93 - 11771 - 95
1818chain 'A' and (resid 118 through 138 )AA118 - 13896 - 105
1919chain 'A' and (resid 139 through 184 )AA139 - 184106 - 151
2020chain 'A' and (resid 185 through 199 )AA185 - 199152 - 166
2121chain 'A' and (resid 200 through 215 )AA200 - 215167 - 182

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