PDB-8j4y: Structure of Mycobacterium tuberculosis NrdF2:NrdIcomplex (reduce...

ID or keywords:

Entry

Database: PDB / ID: 8j4y

Title

Structure of Mycobacterium tuberculosis NrdF2:NrdIcomplex (reduced) determined at 3 angstrom resolution

Components

Protein NrdI
Ribonucleoside-diphosphate reductase subunit beta nrdF2

Keywords

OXIDOREDUCTASE / Ribonucleotide reductase NrdF2 / NrdI protein complex / Metal cofactor assembly

Function / homology

Function and homology information

ribonucleoside-diphosphate reductase complex / ribonucleoside-diphosphate reductase / ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor / deoxyribonucleotide biosynthetic process / protein modification process / FMN binding / DNA replication / metal ion binding
Similarity search - Function

Biological species

Mycobacterium tuberculosis H37Rv (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 3.02 Å

Authors

Yadav, L.R. / Mande, S.C.

Funding support

India, 1items

Citation

Journal: Curr Res Struct Biol / Year: 2024
Title: Structural insights into the initiation of free radical formation in the Class Ib ribonucleotide reductases in Mycobacteria.
Authors: Yadav, L.R. / Sharma, V. / Shanmugam, M. / Mande, S.C.

History

Deposition	Apr 21, 2023	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Oct 9, 2024	Provider: repository / Type: Initial release
Revision 1.1	Oct 30, 2024	Group: Database references / Category: citation Item: _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title

Structure viewer	Molecule: MolmilJmol/JSmol

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/j4/8j4y ftp://data.pdbj.org/pub/pdb/validation_reports/j4/8j4y	HTTPS FTP

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Related structure data

Related structure data	8j4vC 8j4wC 8j4xC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#238 - Oct 2019 Ribonucleotide Reductase similarity (7) #187 - Jul 2015 New Delhi Metallo-β-Lactamase similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #57 - Sep 2004 Catalase similarity (1) #142 - Oct 2011 PDB Pioneers similarity (1)

Deposited unit

A: Ribonucleoside-diphosphate reductase subunit beta nrdF2

B: Ribonucleoside-diphosphate reductase subunit beta nrdF2

C: Ribonucleoside-diphosphate reductase subunit beta nrdF2

D: Ribonucleoside-diphosphate reductase subunit beta nrdF2

E: Ribonucleoside-diphosphate reductase subunit beta nrdF2

F: Ribonucleoside-diphosphate reductase subunit beta nrdF2

G: Protein NrdI

I: Protein NrdI

hetero molecules

257 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Ribonucleoside-diphosphate reductase subunit beta nrdF2

C: Ribonucleoside-diphosphate reductase subunit beta nrdF2

G: Protein NrdI

hetero molecules

defined by author&software
Evidence: light scattering, SEC-MALS analysis indicates the protein to be complex of dimer of NrdF2 that interacts with 1 molecule of NrdI
91.2 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

B: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

defined by author&software
74.2 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

D: Ribonucleoside-diphosphate reductase subunit beta nrdF2

F: Ribonucleoside-diphosphate reductase subunit beta nrdF2

I: Protein NrdI

hetero molecules

defined by author&software
91.3 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

E: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

E: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

defined by author&software
74.2 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	160.430, 160.430, 310.400
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	94
Space group name H-M	P4₂2₁2
Space group name Hall	P4n2n
Symmetry operation	#1: x,y,z #2: -y+1/2,x+1/2,z+1/2 #3: y+1/2,-x+1/2,z+1/2 #4: x+1/2,-y+1/2,-z+1/2 #5: -x+1/2,y+1/2,-z+1/2 #6: -x,-y,z #7: y,x,-z #8: -y,-x,-z

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
d_1	ens_1	(chain "A" and resid 11 through 301)
d_2	ens_1	(chain "B" and (resid 11 through 292 or resid 301))
d_3	ens_1	(chain "C" and resid 11 through 301)
d_4	ens_1	(chain "D" and resid 11 through 301)
d_5	ens_1	(chain "E" and resid 11 through 301)
d_6	ens_1	(chain "F" and (resid 11 through 292 or resid 302))
d_1	ens_2	chain "G"
d_2	ens_2	(chain "I" and (resid 7 through 55 or resid 62 through 141))

NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
d_1	1	ens_1	VAL	VAL	SER	SER	A	A	11 - 292	11 - 292
d_2	1	ens_1	VAL	VAL	SER	SER	B	B	11 - 292	11 - 292
d_3	1	ens_1	VAL	VAL	SER	SER	C	C	11 - 292	11 - 292
d_4	1	ens_1	VAL	VAL	SER	SER	D	D	11 - 292	11 - 292
d_5	1	ens_1	VAL	VAL	SER	SER	E	E	11 - 292	11 - 292
d_6	1	ens_1	VAL	VAL	SER	SER	F	F	11 - 292	11 - 292
d_1	1	ens_2	SER	SER	THR	THR	G	G	7 - 141	7 - 141
d_2	1	ens_2	SER	SER	ARG	ARG	I	H	7 - 55	7 - 55
d_2	2	ens_2	ALA	ALA	THR	THR	I	H	62 - 141	62 - 141

NCS ensembles :

ID
ens_1
ens_2

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Protein , 2 types, 8 molecules ABCDEFGI

#1: Protein	Ribonucleoside-diphosphate reductase subunit beta nrdF2 / Ribonucleoside-diphosphate reductase nrdF2 / Ribonucleotide reductase R2-2 small subunit Mass: 36994.289 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: Ribonucleotide reductase NrdF2 metallocofactor subunit essential for the initiation of nucleotide reduction Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria) Strain: H37Rv / Gene: nrdF2 / Plasmid: pET32a+ / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: P9WH71, ribonucleoside-diphosphate reductase
#2: Protein	Protein NrdI Mass: 16508.662 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Ribonucleotide reductase NrdI subunit essential for meta cofactor assembly Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria) Strain: H37Rv / Gene: nrdI / Plasmid: pET32a+ / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P9WIZ3

#1: Protein

Ribonucleoside-diphosphate reductase subunit beta nrdF2 / Ribonucleoside-diphosphate reductase nrdF2 / Ribonucleotide reductase R2-2 small subunit

Mass: 36994.289 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Details: Ribonucleotide reductase NrdF2 metallocofactor subunit essential for the initiation of nucleotide reduction
Source: (gene. exp.)

Mycobacterium tuberculosis H37Rv (bacteria)
Strain: H37Rv / Gene: nrdF2 / Plasmid: pET32a+ / Production host:

Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3)
References: UniProt: P9WH71, ribonucleoside-diphosphate reductase

#2: Protein

Protein NrdI

Mass: 16508.662 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Ribonucleotide reductase NrdI subunit essential for meta cofactor assembly
Source: (gene. exp.)

Mycobacterium tuberculosis H37Rv (bacteria)
Strain: H37Rv / Gene: nrdI / Plasmid: pET32a+ / Production host:

Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P9WIZ3

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Non-polymers , 5 types, 37 molecules

#3: Chemical	ChemComp-MN / MANGANESE (II) ION Mass: 54.938 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: Mn / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical	ChemComp-PER / PEROXIDE ION Mass: 31.999 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: O₂ / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical	ChemComp-OH / HYDROXIDE ION Mass: 17.007 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: HO / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical	ChemComp-FMN / FLAVIN MONONUCLEOTIDE / RIBOFLAVIN MONOPHOSPHATE Mass: 456.344 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: C₁₇H₂₁N₄O₉P / Feature type: SUBJECT OF INVESTIGATION
#7: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y
Has protein modification	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.92 Å³/Da / Density % sol: 68.59 %
Crystal grow	Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.1M MES pH 6, 1.4M NaCl, 0.075M sodium phosphate monobasic, 0.075M potassium phosphate monobasic Temp details: 293.15

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Data collection

Diffraction	Mean temperature: 100 K / Ambient temp details: 100 / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å
Detector	Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 11, 2018
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9763 Å / Relative weight: 1
Reflection	Resolution: 3.02→91.58 Å / Num. obs: 80104 / % possible obs: 99.9 % / Redundancy: 47.1 % / B_iso Wilson estimate: 53.01 Å² / CC_1/2: 0.912 / CC star: 0.977 / Net I/σ(I): 9.7
Reflection shell	Resolution: 3.02→3.128 Å / Redundancy: 46.8 % / Num. unique obs: 7862 / CC_1/2: 0.39 / CC star: 0.749 / % possible all: 99.95

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Processing

Software

Name	Version	Classification
PHENIX	1.20.1_4487	refinement
PHENIX	1.20.1_4487	refinement
iMOSFLM		data reduction
Aimless		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 3.02→91.58 Å / SU ML: 0.4525 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.092
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2662	4028	5.03 %
R_work	0.2296	75984	-
obs	0.2314	80012	99.88 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 60.23 Å²

Refinement step

Cycle: LAST / Resolution: 3.02→91.58 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	15852	0	84	16	15952

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0025	16276
X-RAY DIFFRACTION	f_angle_d	0.4258	22117
X-RAY DIFFRACTION	f_chiral_restr	0.0347	2448
X-RAY DIFFRACTION	f_plane_restr	0.0039	2829
X-RAY DIFFRACTION	f_dihedral_angle_d	6.3144	2191

Refine LS restraints NCS

Ens-ID	Dom-ID	Asym-ID	Auth asym-ID	Refine-ID	Type	Rms dev position (Å)
ens_1	d_2	A	A	X-RAY DIFFRACTION	Torsion NCS	0.760391535222
ens_1	d_3	A	A	X-RAY DIFFRACTION	Torsion NCS	0.899872578738
ens_1	d_4	A	A	X-RAY DIFFRACTION	Torsion NCS	0.716306652281
ens_1	d_5	A	A	X-RAY DIFFRACTION	Torsion NCS	0.768270112513
ens_1	d_6	A	A	X-RAY DIFFRACTION	Torsion NCS	0.81134109084
ens_2	d_2	G	G	X-RAY DIFFRACTION	Torsion NCS	0.96001983134

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.02-3.06	0.3742	154	0.3517	2527	X-RAY DIFFRACTION	99.96
3.06-3.09	0.3674	158	0.3402	2585	X-RAY DIFFRACTION	99.96
3.09-3.13	0.3634	137	0.3368	2548	X-RAY DIFFRACTION	99.96
3.13-3.17	0.368	136	0.3278	2585	X-RAY DIFFRACTION	100
3.17-3.22	0.4162	139	0.3206	2589	X-RAY DIFFRACTION	100
3.22-3.26	0.3015	154	0.3087	2582	X-RAY DIFFRACTION	100
3.26-3.31	0.3144	120	0.2964	2569	X-RAY DIFFRACTION	100
3.31-3.36	0.3229	109	0.2882	2634	X-RAY DIFFRACTION	100
3.36-3.42	0.3108	122	0.2936	2613	X-RAY DIFFRACTION	100
3.42-3.48	0.3162	127	0.279	2606	X-RAY DIFFRACTION	100
3.48-3.54	0.313	135	0.2727	2596	X-RAY DIFFRACTION	100
3.54-3.61	0.3243	145	0.2578	2560	X-RAY DIFFRACTION	100
3.61-3.68	0.2957	119	0.2523	2618	X-RAY DIFFRACTION	99.96
3.68-3.76	0.2264	143	0.2337	2607	X-RAY DIFFRACTION	99.96
3.76-3.85	0.2761	157	0.2357	2589	X-RAY DIFFRACTION	100
3.85-3.95	0.2815	142	0.2306	2590	X-RAY DIFFRACTION	100
3.95-4.05	0.2863	128	0.2123	2634	X-RAY DIFFRACTION	99.96
4.05-4.17	0.2476	133	0.2081	2611	X-RAY DIFFRACTION	100
4.17-4.31	0.2529	146	0.2153	2593	X-RAY DIFFRACTION	99.96
4.31-4.46	0.2655	161	0.2089	2619	X-RAY DIFFRACTION	100
4.46-4.64	0.229	139	0.2011	2593	X-RAY DIFFRACTION	100
4.64-4.85	0.2122	140	0.1861	2666	X-RAY DIFFRACTION	100
4.85-5.11	0.198	127	0.1828	2618	X-RAY DIFFRACTION	100
5.11-5.43	0.2666	147	0.2002	2659	X-RAY DIFFRACTION	100
5.43-5.84	0.2266	140	0.2063	2654	X-RAY DIFFRACTION	99.93
5.84-6.43	0.2246	127	0.1908	2696	X-RAY DIFFRACTION	100
6.43-7.36	0.2142	138	0.1789	2683	X-RAY DIFFRACTION	99.93
7.36-9.27	0.2089	151	0.1637	2724	X-RAY DIFFRACTION	100
9.28-91.58	0.2084	154	0.1842	2836	X-RAY DIFFRACTION	97.49

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	3.98361720109	0.770616657449	-2.68302763361	0.872753038817	-0.454677342308	4.84435576622	-0.155250897723	-0.680332522887	0.719163410138	0.128207971428	0.194449174089	0.335185779359	-0.650273250258	-0.673198596223	-0.106783712651	0.907979493796	0.262600863674	-0.187644294731	0.695165147553	-0.247345435099	0.472924104627	-32.957	-51.73	-21.492
2	1.19349940727	-0.0771657189523	0.60008717986	0.297882831834	0.314358317905	1.08775777068	-0.377246649511	-0.730665598766	0.273749243838	0.29256690268	0.120964805507	-0.0383454696052	-0.183564517078	-0.59271574662	0.122925522885	0.739128371459	0.259194858173	0.0414190488095	0.651799400913	-0.196442892482	0.517458836191	-44.314	-59.923	-28.416
3	3.51407059564	-0.798331555297	-1.92436660919	3.80421527006	1.91415224893	3.20743264562	-0.0257148150199	0.0988228705126	0.199437609115	-0.544513788122	-0.110431632301	-0.00993806734284	-0.0549881598752	-0.231277226521	0.0445615783307	0.580071578758	0.0481374856511	-0.0315910455902	0.0998811156314	-0.000378878050619	0.574439326441	-82.505	-71.086	-60.83
4	2.41222301177	-0.045215827866	0.599478908086	1.0593632555	0.615299856513	0.603857568407	-0.262169070007	-0.500251596798	0.208209583038	-0.277388174038	0.320361011349	-0.170911308968	-0.296153088275	0.127502627227	-0.0446655520298	0.582651250862	0.0392385036163	0.0701011681178	0.093451821949	-0.0963661807208	0.510834534241	-61.85	-74.641	-54.153
5	8.33790430597	3.77883510095	-1.76792156839	3.19695125977	-1.3465322177	1.61896827465	0.00189122195799	-0.497828129628	0.87286760645	0.255014438751	-0.0197873745358	0.519969491542	-0.492882737858	-0.269576474858	0.0648517028057	1.01938123456	0.226696088675	-0.156270249372	0.496379231365	-0.125354240584	0.344223935351	-16.738	-55.392	-16.511
6	2.67465108888	0.0950700492674	0.810225569416	2.26799572738	0.763859433828	0.932792250183	-0.201221803527	-0.328725580084	0.178369711236	0.39227862279	0.0597690423061	-0.0677616353895	-0.275461773745	-0.0512684278505	0.124887388032	0.736412178779	0.0974770488228	-0.0448445550837	0.453961331867	-0.0548316449749	0.280761112592	-8.603	-65.286	-17.07
7	0.860498062382	-0.237602265695	-0.36598685732	3.8827072624	-1.89027689716	1.81723662553	0.0484328442149	-0.138145812374	0.122452333188	-0.132992565043	-0.176740317479	-0.104850671682	0.220578675775	-0.1435652916	0.123495929435	0.377758968773	0.0231460434067	0.0711916932386	0.133155874033	0.0287883818022	0.408159253616	-20.125	-42.187	-74.899
8	1.34549100556	-0.669933462433	1.2960104459	2.79822378227	-1.50066175966	3.47796838514	0.0279603331091	-0.255767623279	0.45641879945	0.727694352358	0.00736409711363	-0.0373108786341	-0.193110321311	-0.329634232209	0.0434099032413	0.544000223612	0.0375134219391	0.199674384304	0.244357253997	-0.00767763233673	0.602555517897	-23.269	-33.648	-63.512
9	2.92899649403	-1.10872319454	-0.202176315538	4.24140650302	-0.880255329934	0.759729406997	0.290368825235	-0.470094073517	-0.0631959457012	1.69751812939	-0.31617794471	0.147760993111	0.142397307218	-0.0268700424796	-0.0743780728233	1.55080087024	-0.4036248155	-0.0965074651895	0.688493866737	-0.0130493113947	0.663061047951	-2.64	-9.211	-33.421
10	2.10851173999	0.690360253978	-0.0401682403495	3.20274502915	-0.201577456455	1.3908032692	0.405211475884	-0.323586690655	-0.16511970342	1.12503642227	-0.422767604791	0.168020053414	0.0292189324255	0.139494674273	-0.000912706987231	1.27924046894	-0.164015121911	0.0277121666909	0.413578320967	0.0118478523769	0.73325818961	-6.808	-17.477	-43.243
11	6.99182500245	4.11421684518	-2.5029732517	5.83286551709	-1.90404612197	4.53358296455	-0.135360395969	0.295870790005	-0.340838809226	-0.223523813162	0.156687200849	-0.448914766076	0.224725361906	-0.0189036234837	-0.005643098267	0.374263068113	0.0878388178162	-0.0600213522918	0.083243700085	0.0629695205603	0.436610446398	-19.327	-51.713	-84.265
12	2.1649206512	0.804904422316	-0.578960249549	2.02767023118	-0.308866357309	0.914323393245	0.179487956759	-0.0184135179048	-0.394021906836	0.0195167422253	-0.184977943384	-0.266002817473	0.14959724297	0.0459080225225	0.0196938173236	0.414793708208	0.0191063369752	-0.045295210351	0.1582020211	0.0138342284732	0.477740837864	-16.757	-72.508	-78.449
13	1.82916344832	0.0655412630104	-0.34903637123	2.34149469719	0.0226251831522	0.118136730454	-0.155393726951	-0.496405499099	0.075505415162	0.141463394502	0.354184333481	0.0635095673109	-0.369884549716	-0.39654536552	-0.212002211542	0.910957989133	0.235756008096	-0.0310166366619	1.25861925084	-0.102309654189	0.348266117821	-14.897	-64.96	10.893
14	7.24116958888	1.6868434007	1.81513463597	6.73276123689	0.484018622926	8.78388845473	-0.324388812766	1.03894349796	0.17191685371	-1.23897213526	0.275110634477	0.095856205385	0.306072687515	0.175651980431	0.0198607385821	0.636619616114	0.0123082113131	0.0252881087065	0.510500464651	-0.0606045893951	0.539274252341	-15.944	-65.51	-106.941

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 10:57 )	A	10 - 57
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 58:292 )	A	58 - 292
3	X-RAY DIFFRACTION	3	( CHAIN B AND RESID 10:42 )	B	10 - 42
4	X-RAY DIFFRACTION	4	( CHAIN B AND RESID 43:293 )	B	43 - 293
5	X-RAY DIFFRACTION	5	( CHAIN C AND RESID 10:71 )	C	10 - 71
6	X-RAY DIFFRACTION	6	( CHAIN C AND RESID 72:292 )	C	72 - 292
7	X-RAY DIFFRACTION	7	( CHAIN D AND RESID 11:149 )	D	11 - 149
8	X-RAY DIFFRACTION	8	( CHAIN D AND RESID 150:292 )	D	150 - 292
9	X-RAY DIFFRACTION	9	( CHAIN E AND RESID 10:65 )	E	10 - 65
10	X-RAY DIFFRACTION	10	( CHAIN E AND RESID 66:292 )	E	66 - 292
11	X-RAY DIFFRACTION	11	( CHAIN F AND RESID 9:43 )	F	9 - 43
12	X-RAY DIFFRACTION	12	( CHAIN F AND RESID 44:293 )	F	44 - 293
13	X-RAY DIFFRACTION	13	( CHAIN G AND RESID 7:140 )	G	7 - 140
14	X-RAY DIFFRACTION	14	( CHAIN I AND RESID 7:141 )	I	7 - 141

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