PDB-8j4x: Structure of Mycobacterium tuberculosis NrdF2:NrdIcomplex (oxidis...

ID or keywords:

Entry

Database: PDB / ID: 8j4x

Title

Structure of Mycobacterium tuberculosis NrdF2:NrdIcomplex (oxidised) determined at 3 angstrom resolution

Components

Protein NrdI
Ribonucleoside-diphosphate reductase subunit beta nrdF2

Keywords

OXIDOREDUCTASE / Ribonucleotide reductase NrdF2 / NrdI protein complex / Metal cofactor assembly

Function / homology

Function and homology information

ribonucleoside-diphosphate reductase complex / ribonucleoside-diphosphate reductase / ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor / deoxyribonucleotide biosynthetic process / protein modification process / FMN binding / DNA replication / metal ion binding
Similarity search - Function

Biological species

Mycobacterium tuberculosis H37Rv (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 3.04 Å

Authors

Yadav, L.R. / Mande, S.C.

Funding support

India, 1items

Citation

Journal: Curr Res Struct Biol / Year: 2024
Title: Structural insights into the initiation of free radical formation in the Class Ib ribonucleotide reductases in Mycobacteria.
Authors: Yadav, L.R. / Sharma, V. / Shanmugam, M. / Mande, S.C.

History

Deposition	Apr 21, 2023	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Oct 9, 2024	Provider: repository / Type: Initial release
Revision 1.1	Oct 30, 2024	Group: Database references / Category: citation Item: _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title

Structure viewer	Molecule: MolmilJmol/JSmol

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Validation report

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Full document	8j4x_full_validation.pdf.gz	5.5 MB	Display
Data in XML	8j4x_validation.xml.gz	75.5 KB	Display
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Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/j4/8j4x ftp://data.pdbj.org/pub/pdb/validation_reports/j4/8j4x	HTTPS FTP

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Related structure data

Related structure data	8j4vC 8j4wC 8j4yC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#238 - Oct 2019 Ribonucleotide Reductase similarity (7) #187 - Jul 2015 New Delhi Metallo-β-Lactamase similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #57 - Sep 2004 Catalase similarity (1) #142 - Oct 2011 PDB Pioneers similarity (1)

Deposited unit

A: Ribonucleoside-diphosphate reductase subunit beta nrdF2

B: Ribonucleoside-diphosphate reductase subunit beta nrdF2

C: Ribonucleoside-diphosphate reductase subunit beta nrdF2

D: Ribonucleoside-diphosphate reductase subunit beta nrdF2

E: Ribonucleoside-diphosphate reductase subunit beta nrdF2

F: Ribonucleoside-diphosphate reductase subunit beta nrdF2

G: Protein NrdI

I: Protein NrdI

hetero molecules

257 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Ribonucleoside-diphosphate reductase subunit beta nrdF2

C: Ribonucleoside-diphosphate reductase subunit beta nrdF2

G: Protein NrdI

hetero molecules

defined by author&software
Evidence: light scattering, SEC-MALS analysis indicates the protein to be complex of dimer of NrdF2 that interacts with 1 molecule of NrdI
91.3 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

B: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

defined by author&software
74.2 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

D: Ribonucleoside-diphosphate reductase subunit beta nrdF2

F: Ribonucleoside-diphosphate reductase subunit beta nrdF2

I: Protein NrdI

hetero molecules

defined by author&software
91.3 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

E: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

E: Ribonucleoside-diphosphate reductase subunit beta nrdF2

hetero molecules

defined by author&software
74.2 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	160.500, 160.500, 312.080
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	94
Space group name H-M	P4₂2₁2
Space group name Hall	P4n2n
Symmetry operation	#1: x,y,z #2: -y+1/2,x+1/2,z+1/2 #3: y+1/2,-x+1/2,z+1/2 #4: x+1/2,-y+1/2,-z+1/2 #5: -x+1/2,y+1/2,-z+1/2 #6: -x,-y,z #7: y,x,-z #8: -y,-x,-z

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
d_1	ens_1	(chain "A" and resid 11 through 291)
d_2	ens_1	(chain "B" and resid 11 through 291)
d_3	ens_1	(chain "C" and resid 11 through 291)
d_4	ens_1	(chain "D" and resid 11 through 291)
d_5	ens_1	(chain "E" and resid 11 through 291)
d_6	ens_1	(chain "F" and resid 11 through 291)
d_1	ens_2	chain "G"
d_2	ens_2	(chain "I" and (resid 7 through 58 or resid 62 through 141))

NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
d_1	1	ens_1	VAL	VAL	LEU	LEU	A	A	11 - 291	11 - 291
d_2	1	ens_1	VAL	VAL	LEU	LEU	B	B	11 - 291	11 - 291
d_3	1	ens_1	VAL	VAL	LEU	LEU	C	C	11 - 291	11 - 291
d_4	1	ens_1	VAL	VAL	LEU	LEU	D	D	11 - 291	11 - 291
d_5	1	ens_1	VAL	VAL	LEU	LEU	E	E	11 - 291	11 - 291
d_6	1	ens_1	VAL	VAL	LEU	LEU	F	F	11 - 291	11 - 291
d_1	1	ens_2	SER	SER	THR	THR	G	G	7 - 141	7 - 141
d_2	1	ens_2	SER	SER	PRO	PRO	I	H	7 - 58	7 - 58
d_2	2	ens_2	ALA	ALA	THR	THR	I	H	62 - 141	62 - 141

NCS ensembles :

ID
ens_1
ens_2

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Protein , 2 types, 8 molecules ABCDEFGI

#1: Protein	Ribonucleoside-diphosphate reductase subunit beta nrdF2 / Ribonucleoside-diphosphate reductase nrdF2 / Ribonucleotide reductase R2-2 small subunit Mass: 36994.289 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: Ribonucleotide reductase NrdF2 metallocofactor subunit essential for the initiation of nucleotide reduction Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria) Strain: H37Rv / Gene: nrdF2 / Plasmid: pET32a+ / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: P9WH71, ribonucleoside-diphosphate reductase
#2: Protein	Protein NrdI Mass: 16508.662 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Ribonucleotide reductase NrdI metallocofactor assembly subunit essential for the initiation of nucleotide reduction Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria) Strain: H37Rv / Gene: nrdI / Plasmid: pET32a+ / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P9WIZ3

#1: Protein

Ribonucleoside-diphosphate reductase subunit beta nrdF2 / Ribonucleoside-diphosphate reductase nrdF2 / Ribonucleotide reductase R2-2 small subunit

Mass: 36994.289 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Details: Ribonucleotide reductase NrdF2 metallocofactor subunit essential for the initiation of nucleotide reduction
Source: (gene. exp.)

Mycobacterium tuberculosis H37Rv (bacteria)
Strain: H37Rv / Gene: nrdF2 / Plasmid: pET32a+ / Production host:

Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3)
References: UniProt: P9WH71, ribonucleoside-diphosphate reductase

#2: Protein

Protein NrdI

Mass: 16508.662 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Ribonucleotide reductase NrdI metallocofactor assembly subunit essential for the initiation of nucleotide reduction
Source: (gene. exp.)

Mycobacterium tuberculosis H37Rv (bacteria)
Strain: H37Rv / Gene: nrdI / Plasmid: pET32a+ / Production host:

Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P9WIZ3

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Non-polymers , 5 types, 53 molecules

#3: Chemical	ChemComp-MN / MANGANESE (II) ION Mass: 54.938 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: Mn / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical	ChemComp-OH / HYDROXIDE ION Mass: 17.007 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: HO / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₃H₈O₃
#6: Chemical	ChemComp-FMN / FLAVIN MONONUCLEOTIDE / RIBOFLAVIN MONOPHOSPHATE Mass: 456.344 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: C₁₇H₂₁N₄O₉P / Feature type: SUBJECT OF INVESTIGATION
#7: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 31 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y
Has protein modification	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.94 Å³/Da / Density % sol: 68.79 % / Description: square shaped crystals
Crystal grow	Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.1M MES pH 6, 1.4M NaCl, 0.075M sodium phosphate monobasic, 0.075M potassium phosphate monobasic Temp details: 293.15

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Data collection

Diffraction	Mean temperature: 100 K / Ambient temp details: 100 / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å
Detector	Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 11, 2018
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9763 Å / Relative weight: 1
Reflection	Resolution: 3.04→48.37 Å / Num. obs: 149656 / % possible obs: 99.83 % / Redundancy: 20 % / B_iso Wilson estimate: 54.43 Å² / CC_1/2: 0.722 / CC star: 0.916 / Net I/σ(I): 5.6
Reflection shell	Resolution: 3.04→3.149 Å / Num. unique obs: 7797 / CC_1/2: 0.387 / CC star: 0.747

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Processing

Software

Name	Version	Classification
PHENIX	1.20.1_4487	refinement
PHENIX	1.20.1_4487	refinement
iMOSFLM		data reduction
Aimless		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 3.04→48.27 Å / SU ML: 0.4565 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.453
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2536	7524	5.03 %
R_work	0.219	142132	-
obs	0.2207	149656	99.88 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 58.66 Å²

Refinement step

Cycle: LAST / Resolution: 3.04→48.27 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	15901	0	92	31	16024

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0025	16336
X-RAY DIFFRACTION	f_angle_d	0.4366	22205
X-RAY DIFFRACTION	f_chiral_restr	0.0345	2456
X-RAY DIFFRACTION	f_plane_restr	0.0033	2842
X-RAY DIFFRACTION	f_dihedral_angle_d	3.6051	2202

Refine LS restraints NCS

Ens-ID	Dom-ID	Asym-ID	Auth asym-ID	Refine-ID	Type	Rms dev position (Å)
ens_1	d_2	A	A	X-RAY DIFFRACTION	Torsion NCS	0.576465222957
ens_1	d_3	A	A	X-RAY DIFFRACTION	Torsion NCS	0.711236860899
ens_1	d_4	A	A	X-RAY DIFFRACTION	Torsion NCS	0.47183011791
ens_1	d_5	A	A	X-RAY DIFFRACTION	Torsion NCS	0.644573268351
ens_1	d_6	A	A	X-RAY DIFFRACTION	Torsion NCS	0.691009405147
ens_2	d_2	G	G	X-RAY DIFFRACTION	Torsion NCS	1.04329605702

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.04-3.07	0.3604	274	0.3687	4715	X-RAY DIFFRACTION	99.82
3.07-3.11	0.358	309	0.361	4664	X-RAY DIFFRACTION	99.78
3.11-3.15	0.3744	245	0.3568	4788	X-RAY DIFFRACTION	99.8
3.15-3.19	0.4047	249	0.355	4687	X-RAY DIFFRACTION	99.84
3.19-3.23	0.3948	251	0.3433	4708	X-RAY DIFFRACTION	99.98
3.23-3.27	0.3708	275	0.3218	4783	X-RAY DIFFRACTION	100
3.27-3.32	0.3688	222	0.3255	4762	X-RAY DIFFRACTION	99.98
3.32-3.37	0.3103	209	0.3157	4739	X-RAY DIFFRACTION	99.92
3.37-3.42	0.3061	212	0.3072	4805	X-RAY DIFFRACTION	100
3.42-3.48	0.3026	235	0.2986	4759	X-RAY DIFFRACTION	100
3.48-3.54	0.3348	236	0.2778	4775	X-RAY DIFFRACTION	99.98
3.54-3.6	0.2934	267	0.2534	4707	X-RAY DIFFRACTION	99.88
3.6-3.67	0.3143	215	0.2505	4780	X-RAY DIFFRACTION	100
3.67-3.75	0.246	271	0.2259	4712	X-RAY DIFFRACTION	100
3.75-3.83	0.2472	275	0.2206	4726	X-RAY DIFFRACTION	100
3.83-3.92	0.2711	287	0.2035	4689	X-RAY DIFFRACTION	100
3.92-4.02	0.2378	219	0.1912	4796	X-RAY DIFFRACTION	99.98
4.02-4.13	0.2457	236	0.1827	4749	X-RAY DIFFRACTION	100
4.13-4.25	0.2276	278	0.1823	4700	X-RAY DIFFRACTION	100
4.25-4.38	0.2185	262	0.1725	4751	X-RAY DIFFRACTION	100
4.38-4.54	0.1817	274	0.1648	4724	X-RAY DIFFRACTION	100
4.54-4.72	0.1974	239	0.1627	4756	X-RAY DIFFRACTION	99.96
4.72-4.94	0.1943	276	0.1563	4698	X-RAY DIFFRACTION	99.96
4.94-5.2	0.2181	215	0.1723	4777	X-RAY DIFFRACTION	99.96
5.2-5.52	0.2614	233	0.1892	4790	X-RAY DIFFRACTION	99.94
5.52-5.95	0.2276	269	0.1938	4712	X-RAY DIFFRACTION	100
5.95-6.54	0.206	212	0.1803	4754	X-RAY DIFFRACTION	100
6.54-7.49	0.2078	245	0.1715	4747	X-RAY DIFFRACTION	99.96
7.49-9.42	0.1816	264	0.1434	4755	X-RAY DIFFRACTION	100
9.42-48.27	0.1784	270	0.1691	4624	X-RAY DIFFRACTION	97.96

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.4112929384	0.101859473519	-1.76248033506	1.76170943211	0.122247057655	3.09261752874	-0.205874646771	-0.200094214763	0.324676200086	0.236851546277	0.0408540304426	0.320680999422	-0.606881579589	-0.664307597344	0.0738760653429	0.603388326296	0.184729388494	-0.096012075006	0.804726487315	-0.202537160835	0.314632402	-36.465	-53.677	-20.594
2	2.09340662569	-0.226763491347	0.0790020439557	0.621105449779	-0.0544937103745	1.46555573611	-0.196468986954	-0.333964136717	0.123582682398	0.22487667254	0.0626109619885	0.196002943096	-0.234500187815	-0.642227311898	0.122465784733	0.447944545397	0.13379361143	0.0351395996946	0.697238969082	-0.174210582537	0.352762404721	-43.922	-59.686	-29.306
3	4.61010511187	-0.648397990657	0.317462717154	1.6011351389	0.00528957432415	0.136734720508	-0.133021798779	-0.525493171581	0.555056970119	-0.152112823476	0.22015986384	-0.259761532049	-0.180528042872	0.0633552976809	-0.0943178563253	0.368850619269	-0.020338881036	0.0153451906182	0.222600656694	-0.0939733562711	0.298739107532	-67.504	-76.085	-55.806
4	2.68540780912	-1.34126771948	-1.11696902306	7.37383328306	2.05459470856	4.40265322449	-0.131713370782	-0.173164534224	0.560108903793	-0.721544484096	-0.0304058400049	-0.882360473309	-0.227441531947	0.22584576935	0.0261836893036	0.278147188695	-0.0686717022543	0.0252250867991	0.323865796814	-0.210551429734	0.583422974269	-53.392	-67.949	-54.115
5	7.43797670056	3.34080788983	-2.67548140197	3.54705630304	-3.2926745755	3.26266575496	-0.01428126902	-0.875737886763	0.184267765811	0.401187055961	-0.15599092854	-0.00970817645356	-0.222201430292	-0.0154676682068	0.11594472242	0.614114413019	0.185197200091	0.0675876436738	0.71401967985	-0.214443285409	0.393696157551	-28.633	-60.83	-12.077
6	1.55825350779	-0.0534448720255	0.171731285033	2.31591052341	-0.0162130169158	1.26393353875	-0.213823261291	-0.36465318785	0.160455878538	0.321561960258	0.0905728242159	-0.310792430636	-0.256258097179	0.168019477283	0.123387551824	0.390290960848	-0.000911055612727	-0.0478774135488	0.562610242407	-0.0805926720574	0.271824835212	-7.833	-63.589	-17.902
7	0.339388942454	1.39481043168	-0.703395902284	6.46748668849	-3.82279737571	3.42890371673	-0.102395351021	-0.0448052803776	-0.0809170556757	-0.0920513617355	0.431398594185	0.370582277889	0.148516585232	-0.375820000478	-0.268033094252	0.187538986711	0.0892227429909	0.130037476523	0.361612499102	0.0356498262936	0.481536571711	-25.634	-43.938	-75.991
8	0.65410396874	0.296807984161	0.746326367045	1.9918529955	-1.45696961306	1.79655568456	-0.00693912305684	-0.17122420973	0.359542015558	0.260737306914	-0.0898784216941	0.0879659066879	-0.303765219196	-0.210821512348	0.0808646536596	0.374472364436	0.0640390996581	0.16463944582	0.305490936893	0.00324091133331	0.57280254671	-20.688	-36.317	-67.467
9	1.96855445902	1.46989907954	-0.0889755422041	6.42980591431	-0.44234151833	1.37512867532	0.715598395521	-0.614959075015	-0.117317760058	2.01268728525	-0.979396716353	0.271474319533	0.0228688161908	0.0598418099014	0.185773806746	1.29535885183	-0.315767478354	0.0987214365183	0.622208969237	0.0580216164638	0.69733852994	-4.402	-13.815	-41.861
10	8.39367400062	0.235804678797	0.0985452185962	0.654777635146	1.94372430403	5.54289508616	0.705658698717	-1.106597703	-0.501587783762	1.3697969378	-0.901117067823	0.747656586219	1.03322772641	-0.598281091416	0.216764075767	1.39926709154	-0.352764663835	0.41799355786	0.696749805702	-0.141487704503	1.11917627726	-14.103	-26.891	-39.871
11	3.53810670325	3.02594090719	-3.98321950109	9.203550919	-0.789332317011	5.55696603446	0.0334388399116	0.488461850107	0.389185086275	-0.226888724196	0.585070014028	-0.352628949974	-0.313671545775	0.100816039915	-0.613406505973	0.259878564825	0.132520410935	0.0503473423563	0.215434789551	0.0462291856629	0.363359043525	-15.78	-49.24	-85.379
12	2.23906355333	0.970837548986	-0.117791641318	3.06109691699	-0.0408108932392	1.37644398018	0.167435133538	-0.010021090237	-0.383192553605	-0.0905803117979	-0.0523847584149	0.0236161381953	0.163761780358	-0.0607126384244	-0.100734943796	0.246026160712	0.0288368997387	-0.0351342316629	0.262702834017	0.0451809323737	0.514612572559	-17.183	-72.313	-78.942
13	1.45066638565	1.52309306966	0.416640482306	3.09160240257	1.0307116167	3.54540799182	-0.263263769081	-0.972378725342	0.104058307488	0.780919664869	0.167170817919	0.00673290929447	-0.0618657492189	-0.0740334747911	0.121670366972	0.73804305226	0.19020034662	-0.0638429021052	1.24167485462	-0.0985784078629	0.39681578804	-15.068	-64.729	10.633
14	6.17076488182	1.72370069723	0.299861203577	3.68366048958	0.481690906508	9.83503259641	-0.257360518716	0.967196324608	-0.0648786358857	-1.24959728757	0.224521392164	-0.0114272747695	-0.000565369497124	-0.126242558636	-0.04248438888	0.726144755384	0.0606612031044	0.000636589682938	0.452280894805	-0.0149963844096	0.415415197177	-15.907	-65.477	-107.674

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 10:68 )	A	10 - 68
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 69:293 )	A	69 - 293
3	X-RAY DIFFRACTION	3	( CHAIN B AND RESID 11:228 )	B	11 - 228
4	X-RAY DIFFRACTION	4	( CHAIN B AND RESID 229:292 )	B	229 - 292
5	X-RAY DIFFRACTION	5	( CHAIN C AND RESID 9:43 )	C	9 - 43
6	X-RAY DIFFRACTION	6	( CHAIN C AND RESID 44:291 )	C	44 - 291
7	X-RAY DIFFRACTION	7	( CHAIN D AND RESID 9:72 )	D	9 - 72
8	X-RAY DIFFRACTION	8	( CHAIN D AND RESID 73:293 )	D	73 - 293
9	X-RAY DIFFRACTION	9	( CHAIN E AND RESID 11:244 )	E	11 - 244
10	X-RAY DIFFRACTION	10	( CHAIN E AND RESID 245:292 )	E	245 - 292
11	X-RAY DIFFRACTION	11	( CHAIN F AND RESID 8:37 )	F	8 - 37
12	X-RAY DIFFRACTION	12	( CHAIN F AND RESID 38:293 )	F	38 - 293
13	X-RAY DIFFRACTION	13	( CHAIN G AND RESID 7:141 )	G	7 - 141
14	X-RAY DIFFRACTION	14	( CHAIN I AND RESID 7:143 )	I	7 - 143

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