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- PDB-8izt: Crystal structure of the N-terminal domain (residues 1-121) of MPXV A7 -
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Open data
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Basic information
Entry | Database: PDB / ID: 8izt | ||||||
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Title | Crystal structure of the N-terminal domain (residues 1-121) of MPXV A7 | ||||||
![]() | Virion morphogenesis protein OPG132 | ||||||
![]() | VIRAL PROTEIN / MPXV / A7 / N-terminus | ||||||
Function / homology | Poxvirus A6 / Poxvirus A6 protein / virion component / host cell cytoplasm / Virion morphogenesis protein OPG132![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ni, X.C. / Lei, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural analysis of conformational changes in the mpox virus A7 protein. Authors: Ni, X. / Wang, K. / Han, Y. / Lei, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.2 KB | Display | ![]() |
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PDB format | ![]() | 27.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 415.5 KB | Display | ![]() |
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Full document | ![]() | 415.5 KB | Display | |
Data in XML | ![]() | 8.3 KB | Display | |
Data in CIF | ![]() | 11.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8izuC ![]() 6cb6S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14280.537 Da / Num. of mol.: 1 / Fragment: N-terminal domain (residues 1-121) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.92 Å3/Da / Density % sol: 35.91 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 0,01 M Zinc chloride, 0.1 M MES pH 6.0, 20% w/v PEG6000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 15, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→35.1 Å / Num. obs: 10782 / % possible obs: 99.7 % / Redundancy: 6.2 % / CC1/2: 0.999 / Net I/σ(I): 15 |
Reflection shell | Resolution: 1.74→1.77 Å / Num. unique obs: 612 / CC1/2: 0.255 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6CB6 Resolution: 1.74→35.1 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.95 / SU B: 3.553 / SU ML: 0.108 / Cross valid method: THROUGHOUT / ESU R: 0.14 / ESU R Free: 0.124 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.466 Å2
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Refinement step | Cycle: LAST / Resolution: 1.74→35.1 Å
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Refine LS restraints |
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LS refinement shell |
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