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- PDB-8is2: Crystal structure of a polyketide aromatase/cyclase Abx(+)D from ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8is2 | ||||||
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Title | Crystal structure of a polyketide aromatase/cyclase Abx(+)D from Actinomycetes sp. MA7150. | ||||||
![]() | Cyclase | ||||||
![]() | BIOSYNTHETIC PROTEIN / aromatase / cyclase / polyketide | ||||||
Function / homology | Polyketide synthesis cyclase / Polyketide synthesis cyclase superfamily / Polyketide synthesis cyclase / polyketide biosynthetic process / Dimeric alpha-beta barrel / Cyclase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Luo, S. / Chen, X. | ||||||
Funding support | ![]()
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![]() | ![]() Title: An unusual aromatase/cyclase programs the formation of the phenyldimethylanthrone framework in anthrabenzoxocinones and fasamycin. Authors: Jiang, K. / Chen, X. / Yan, X. / Li, G. / Lin, Z. / Deng, Z. / Luo, S. / Qu, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 103.9 KB | Display | ![]() |
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PDB format | ![]() | 78.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 409.7 KB | Display | ![]() |
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Full document | ![]() | 409.6 KB | Display | |
Data in XML | ![]() | 5.8 KB | Display | |
Data in CIF | ![]() | 8.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13440.099 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The sequence reference for Actinomycetes sp. MA7150 is not available at the time of biocuration. Current reference is A0A2U3TEK9. Source: (gene. exp.) ![]() Gene: abxD / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.78 % |
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Crystal grow | Temperature: 293.15 K / Method: liquid diffusion / pH: 8.1 / Details: 0.25 M Hepes, 60% Tacsimate, 0.17 M NDSB-211 |
-Data collection
Diffraction | Mean temperature: 298 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 6, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979191 Å / Relative weight: 1 |
Reflection | Resolution: 1.32→38 Å / Num. obs: 49575 / % possible obs: 97.5 % / Redundancy: 2.6 % / CC1/2: 0.999 / Rmerge(I) obs: 0.026 / Net I/σ(I): 9.2 |
Reflection shell | Resolution: 1.32→1.39 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.38 / Mean I/σ(I) obs: 2.6 / Num. unique obs: 7369 / CC1/2: 0.765 / % possible all: 98.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.32→21.05 Å
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Refine LS restraints |
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LS refinement shell |
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