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Open data
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Basic information
Entry | Database: PDB / ID: 8i5m | ||||||
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Title | Rat Kir4.1 in complex with PIP2 | ||||||
![]() | ATP-sensitive inward rectifier potassium channel 10 | ||||||
![]() | MEMBRANE PROTEIN / kir4.1 / ion channel / transport protein | ||||||
Function / homology | ![]() glutamate reuptake / Potassium transport channels / L-glutamate import / response to blue light / central nervous system myelination / response to mineralocorticoid / regulation of resting membrane potential / cellular response to potassium ion / oligodendrocyte development / astrocyte projection ...glutamate reuptake / Potassium transport channels / L-glutamate import / response to blue light / central nervous system myelination / response to mineralocorticoid / regulation of resting membrane potential / cellular response to potassium ion / oligodendrocyte development / astrocyte projection / inward rectifier potassium channel activity / potassium ion homeostasis / non-motile cilium assembly / membrane hyperpolarization / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / adult walking behavior / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / ciliary base / optic nerve development / potassium channel activity / potassium ion import across plasma membrane / microvillus / response to glucocorticoid / potassium ion transmembrane transport / visual perception / establishment of localization in cell / regulation of membrane potential / regulation of long-term neuronal synaptic plasticity / potassium ion transport / presynapse / cell body / basolateral plasma membrane / apical plasma membrane / signaling receptor binding / ATP binding / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.85 Å | ||||||
![]() | Zhao, C. / Guo, J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Pharmacological inhibition of astrocytic Kir4.1 channel evokes rapid-onset antidepressant responses Authors: Zhao, C. / Guo, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 420.5 KB | Display | ![]() |
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PDB format | ![]() | 349.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 44.7 KB | Display | |
Data in CIF | ![]() | 55.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 35195MC ![]() 8i5nC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 38791.723 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-PIO / [( Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Rat Kir4.1 in complex with PIP2 / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: DARK FIELD / Nominal defocus max: 1400 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 62 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 2.85 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 22786 / Symmetry type: POINT | ||||||||||||||||||||||||
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