+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35196 | |||||||||
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Title | Rat Kir4.1 in complex with PIP2 and Lys05 | |||||||||
Map data | ||||||||||
Sample |
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Keywords | kir4.1 / ion channel / transport protein / MEMBRANE PROTEIN | |||||||||
Function / homology | Function and homology information glutamate reuptake / Potassium transport channels / L-glutamate import / response to blue light / central nervous system myelination / regulation of resting membrane potential / response to mineralocorticoid / cellular response to potassium ion / oligodendrocyte development / astrocyte projection ...glutamate reuptake / Potassium transport channels / L-glutamate import / response to blue light / central nervous system myelination / regulation of resting membrane potential / response to mineralocorticoid / cellular response to potassium ion / oligodendrocyte development / astrocyte projection / inward rectifier potassium channel activity / potassium ion homeostasis / membrane hyperpolarization / non-motile cilium assembly / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / optic nerve development / adult walking behavior / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / ciliary base / potassium ion import across plasma membrane / microvillus / potassium channel activity / response to glucocorticoid / potassium ion transmembrane transport / visual perception / regulation of membrane potential / establishment of localization in cell / regulation of long-term neuronal synaptic plasticity / potassium ion transport / presynapse / cell body / basolateral plasma membrane / apical plasma membrane / signaling receptor binding / ATP binding / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Rattus norvegicus (Norway rat) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.85 Å | |||||||||
Authors | Zhao C / Guo J | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Pharmacological inhibition of astrocytic Kir4.1 channel evokes rapid-onset antidepressant responses Authors: Zhao C / Guo J | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35196.map.gz | 17.1 MB | EMDB map data format | |
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Header (meta data) | emd-35196-v30.xml emd-35196.xml | 13 KB 13 KB | Display Display | EMDB header |
Images | emd_35196.png | 116 KB | ||
Filedesc metadata | emd-35196.cif.gz | 5.4 KB | ||
Others | emd_35196_half_map_1.map.gz emd_35196_half_map_2.map.gz | 17.1 MB 17 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35196 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35196 | HTTPS FTP |
-Related structure data
Related structure data | 8i5nMC 8i5mC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_35196.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.014 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_35196_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35196_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Rat Kir4.1 in complex with PIP2 and Lys05
Entire | Name: Rat Kir4.1 in complex with PIP2 and Lys05 |
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Components |
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-Supramolecule #1: Rat Kir4.1 in complex with PIP2 and Lys05
Supramolecule | Name: Rat Kir4.1 in complex with PIP2 and Lys05 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Rattus norvegicus (Norway rat) |
-Macromolecule #1: ATP-sensitive inward rectifier potassium channel 10
Macromolecule | Name: ATP-sensitive inward rectifier potassium channel 10 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: Rattus norvegicus (Norway rat) |
Molecular weight | Theoretical: 38.791723 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: GAPGIRRRRV LTKDGRSNVR MEHIADKRFL YLKDLWTTFI DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG PPANHTPCV VQVHTLTGAF LFSLESQTTI GYGFRYISEE CPLAIVLLIA QLVLTTILEI FITGTFLAKI ARPKKRAETI R FSQHAVVA ...String: GAPGIRRRRV LTKDGRSNVR MEHIADKRFL YLKDLWTTFI DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG PPANHTPCV VQVHTLTGAF LFSLESQTTI GYGFRYISEE CPLAIVLLIA QLVLTTILEI FITGTFLAKI ARPKKRAETI R FSQHAVVA YHNGKLCLMI RVANMRKSLL IGCQVTGKLL QTHQTKEGEN IRLNQVNVTF QVDTASDSPF LILPLTFYHV VD ETSPLKD LPLRSGEGDF ELVLILSGTV ESTSATCQVR TSYLPEEILW GYEFTPAISL SASGKYVADF SLFDQVVKVA SPG GLRDST VRYGDPEKLK LDYKDDDDK UniProtKB: ATP-sensitive inward rectifier potassium channel 10 |
-Macromolecule #2: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(o...
Macromolecule | Name: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate type: ligand / ID: 2 / Number of copies: 4 / Formula: PIO |
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Molecular weight | Theoretical: 746.566 Da |
Chemical component information | ChemComp-PIO: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: DARK FIELD / Nominal defocus max: 1.4000000000000001 µm / Nominal defocus min: 1.2 µm |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 62.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: OTHER |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.85 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 75833 |