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Open data
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Basic information
Entry | Database: PDB / ID: 8hq1 | ||||||
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Title | Crystal Structure Of Human Lgi1-Adam22 Complex In Space Group C2 | ||||||
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![]() | MEMBRANE PROTEIN / SYNAPTIC MODULATOR / COMPLEX | ||||||
Function / homology | ![]() LGI-ADAM interactions / axon initial segment / negative regulation of cell adhesion / neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of synaptic transmission / synaptic cleft / central nervous system development / axon guidance / postsynaptic density membrane / metalloendopeptidase activity ...LGI-ADAM interactions / axon initial segment / negative regulation of cell adhesion / neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of synaptic transmission / synaptic cleft / central nervous system development / axon guidance / postsynaptic density membrane / metalloendopeptidase activity / integrin binding / neuron projection development / nervous system development / positive regulation of cell growth / cell adhesion / axon / signaling receptor binding / glutamatergic synapse / dendrite / Golgi apparatus / endoplasmic reticulum / proteolysis / extracellular space / extracellular region / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, H.L. / Lin, Z.H. / Xu, F. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure Of Human Lgi1-Adam22 Complex In Space Group C2 Authors: Liu, H.L. / Lin, Z.H. / Xu, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 605.3 KB | Display | ![]() |
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PDB format | ![]() | 495.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 482.7 KB | Display | ![]() |
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Full document | ![]() | 537.1 KB | Display | |
Data in XML | ![]() | 63.5 KB | Display | |
Data in CIF | ![]() | 93 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1w8aS ![]() 3g5cS ![]() 5sxmS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 53743.102 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein | Mass: 60616.598 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #3: Sugar | ChemComp-NAG / #4: Chemical | ChemComp-CA / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.04 Å3/Da / Density % sol: 75.61 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.2 Details: 2M SODIUM CHLORIDE, 0.1M POTASSIUM/SODIUM PHOSPHATE pH=6.2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: May 15, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 4.17→50 Å / Num. obs: 50134 / % possible obs: 99.5 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.074 / Rsym value: 0.04 / Net I/σ(I): 19.667 |
Reflection shell | Resolution: 4.17→4.31 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.758 / Mean I/σ(I) obs: 1.909 / Num. unique obs: 4928 / Rsym value: 0.502 / % possible all: 98.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1W8A, 5SXM, 3G5C Resolution: 4.17→37.92 Å / SU ML: 0.56 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 40.68
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.17→37.92 Å
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Refine LS restraints |
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LS refinement shell |
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