+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8gxs | ||||||
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タイトル | PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state | ||||||
要素 |
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キーワード | TRANSCRIPTION / PIC / Mediator / transcription initiation / +1 nucleosome | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / spermine transport / negative regulation of MHC class I biosynthetic process / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / MMXD complex / core TFIIH complex portion of holo TFIIH complex ...positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / spermine transport / negative regulation of MHC class I biosynthetic process / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / MMXD complex / core TFIIH complex portion of holo TFIIH complex / SAGA complex assembly / lateral mesodermal cell differentiation / androgen biosynthetic process / positive regulation of G0 to G1 transition / DNA-templated transcription open complex formation / allantois development / mammary gland branching involved in thelarche / retinal pigment epithelium development / pre-snoRNP complex / Cytosolic iron-sulfur cluster assembly / nucleotide-excision repair, DNA duplex unwinding / TFIIH-class transcription factor complex binding / G0 to G1 transition / thyroid hormone receptor signaling pathway / negative regulation of protein autoubiquitination / central nervous system myelin formation / positive regulation of core promoter binding / transcription factor TFTC complex / RNA polymerase II core complex assembly / core mediator complex / positive regulation of mitotic recombination / meiotic sister chromatid cohesion / negative regulation of MHC class II biosynthetic process / hair follicle maturation / RNA polymerase transcription factor SL1 complex / ventricular system development / snRNA transcription by RNA polymerase II / hair cell differentiation / regulation of cell cycle G1/S phase transition / RNA polymerase I general transcription initiation factor activity / regulation of vitamin D receptor signaling pathway / nucleotide-excision repair factor 3 complex / transcription factor TFIIE complex / phosphatase activator activity / nucleotide-excision repair, preincision complex assembly / SLIK (SAGA-like) complex / CAK-ERCC2 complex / RNA polymerase III general transcription initiation factor activity / UV protection / transcription factor TFIIK complex / embryonic cleavage / transcription open complex formation at RNA polymerase II promoter / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / RNA polymerase I core promoter sequence-specific DNA binding / TFIIF-class transcription factor complex binding / hepatocyte differentiation / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / transcriptional start site selection at RNA polymerase II promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / ventricular trabecula myocardium morphogenesis / positive regulation of response to cytokine stimulus / thyroid hormone generation / transcription factor TFIIF complex / DNA 5'-3' helicase / mediator complex / nuclear retinoic acid receptor binding / maintenance of protein location in nucleus / RNA Polymerase III Abortive And Retractive Initiation / adult heart development / positive regulation of androgen receptor activity / positive regulation of keratinocyte differentiation / Generic Transcription Pathway 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) Xenopus laevis (アフリカツメガエル) Sus scrofa (ブタ) synthetic construct (人工物) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.16 Å | ||||||
データ登録者 | Chen, X. / Wang, X. / Liu, W. / Ren, Y. / Qu, X. / Li, J. / Yin, X. | ||||||
資金援助 | 1件
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引用 | ジャーナル: Science / 年: 2022 タイトル: Structures of +1 nucleosome-bound PIC-Mediator complex. 著者: Xizi Chen / Xinxin Wang / Weida Liu / Yulei Ren / Xuechun Qu / Jiabei Li / Xiaotong Yin / Yanhui Xu / 要旨: RNA polymerase II-mediated eukaryotic transcription starts with the assembly of the preinitiation complex (PIC) on core promoters. The +1 nucleosome is well positioned about 40 base pairs downstream ...RNA polymerase II-mediated eukaryotic transcription starts with the assembly of the preinitiation complex (PIC) on core promoters. The +1 nucleosome is well positioned about 40 base pairs downstream of the transcription start site (TSS) and is commonly known as a barrier of transcription. The +1 nucleosome-bound PIC-Mediator structures show that PIC-Mediator prefers binding to T40N nucleosome located 40 base pairs downstream of TSS and contacts T50N but not the T70N nucleosome. The nucleosome facilitates the organization of PIC-Mediator on the promoter by binding TFIIH subunit p52 and Mediator subunits MED19 and MED26 and may contribute to transcription initiation. PIC-Mediator exhibits multiple nucleosome-binding patterns, supporting a structural role of the +1 nucleosome in the coordination of PIC-Mediator assembly. Our study reveals the molecular mechanism of PIC-Mediator organization on chromatin and underscores the significance of the +1 nucleosome in regulating transcription initiation. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8gxs.cif.gz | 4.3 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8gxs.ent.gz | 表示 | PDB形式 | |
PDBx/mmJSON形式 | 8gxs.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8gxs_validation.pdf.gz | 1.8 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8gxs_full_validation.pdf.gz | 1.9 MB | 表示 | |
XML形式データ | 8gxs_validation.xml.gz | 460.7 KB | 表示 | |
CIF形式データ | 8gxs_validation.cif.gz | 783 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/gx/8gxs ftp://data.pdbj.org/pub/pdb/validation_reports/gx/8gxs | HTTPS FTP |
-関連構造データ
関連構造データ | 34360MC 8gxqC C: 同じ文献を引用 (文献) M: このデータのモデリングに利用したマップデータ |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
+DNA鎖 , 4種, 4分子 XYNXNY
+Transcription initiation factor ... , 17種, 23分子 BADADBEBDODQDDDdDEDeDFDfDGDHDIDiDLDlDcDjDJDkDm
+タンパク質 , 11種, 15分子 EANANENBNFDPPKPLHDHIHJNCNGNDNH
+General transcription factor IIF subunit ... , 2種, 2分子 FAFB
+General transcription and DNA repair factor IIH helicase subunit ... , 2種, 2分子 HAHH
+DNA-directed RNA polymerase ... , 8種, 8分子 PAPBPCPEPFPIPDPG
+General transcription factor IIH subunit ... , 5種, 5分子 HBHCHGHEHF
+DNA-directed RNA polymerases I, II, and III subunit ... , 2種, 2分子 PHPJ
+Mediator of RNA polymerase II transcription subunit ... , 24種, 24分子 gjnsuadfimqzbcelokrtvwxy
+Isoform 2 of Mediator of RNA polymerase II transcription subunit ... , 2種, 2分子 hp
+非ポリマー , 3種, 21分子
+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state タイプ: COMPLEX / Entity ID: #1-#77 / 由来: MULTIPLE SOURCES |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
由来(組換発現) | 生物種: Homo sapiens (ヒト) |
緩衝液 | pH: 7.9 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2500 nm / 最小 デフォーカス(公称値): 1500 nm |
撮影 | 電子線照射量: 50 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
-解析
CTF補正 | タイプ: NONE |
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3次元再構成 | 解像度: 4.16 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 29473 / 対称性のタイプ: POINT |