EMDB-34360: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state

Basic information

Entry

Database: EMDB / ID: EMD-34360

• Title: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
• Map data: PIC-Mediator in complex with plus 1 nucleosome (T40N) in H-binding state

Sample

• Complex: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
  • DNA: x 4 types
  • Protein or peptide: x 73 types
• Ligand: x 3 types

• Keywords: PIC / Mediator / transcription initiation / +1 nucleosome / TRANSCRIPTION

Function / homology

Function:

positive regulation of mediator complex assembly / positive regulation of T cell extravasation / negative regulation of smooth muscle cell differentiation / negative regulation of MHC class I biosynthetic process / spermine transport / MMXD complex / core TFIIH complex portion of holo TFIIH complex / SAGA complex assembly / lateral mesodermal cell differentiation / enucleate erythrocyte development / regulation of RNA biosynthetic process / DNA-templated transcription open complex formation / allantois development / positive regulation of type II interferon-mediated signaling pathway / pre-snoRNP complex / positive regulation of androgen receptor signaling pathway / Cytosolic iron-sulfur cluster assembly / androgen biosynthetic process / positive regulation of G0 to G1 transition / central nervous system myelin formation / TFIIH-class transcription factor complex binding / negative regulation of protein autoubiquitination / retinal pigment epithelium development / G0 to G1 transition / positive regulation of core promoter binding / mammary gland branching involved in thelarche / RNA polymerase II CTD heptapeptide repeat Y1 kinase activity / RNA polymerase II CTD heptapeptide repeat S2 kinase activity / RNA polymerase II CTD heptapeptide repeat T4 kinase activity / RNA polymerase II CTD heptapeptide repeat S5 kinase activity / RNA polymerase II CTD heptapeptide repeat S7 kinase activity / thyroid hormone receptor signaling pathway / transcription factor TFTC complex / RNA polymerase II core complex assembly / positive regulation of mitotic recombination / hair cell differentiation / hair follicle maturation / RNA polymerase I general transcription initiation factor activity / regulation of cell cycle G1/S phase transition / ventricular system development / nucleotide-excision repair factor 3 complex / core mediator complex / transcription factor TFIIE complex / RNA polymerase transcription factor SL1 complex / nucleotide-excision repair, preincision complex assembly / meiotic sister chromatid cohesion / phosphatase activator activity / snRNA transcription by RNA polymerase II / regulation of vitamin D receptor signaling pathway / SLIK (SAGA-like) complex / histone H4K16ac reader activity / CAK-ERCC2 complex / negative regulation of MHC class II biosynthetic process / transcription factor TFIIK complex / RNA polymerase III general transcription initiation factor activity / TFIIF-class transcription factor complex binding / UV protection / transcriptional start site selection at RNA polymerase II promoter / RNA polymerase I core promoter sequence-specific DNA binding / hepatocyte differentiation / nuclear retinoic acid receptor binding / transcription factor TFIIF complex / embryonic cleavage / positive regulation of response to cytokine stimulus / ventricular trabecula myocardium morphogenesis / adult heart development / mediator complex / DNA 5'-3' helicase / positive regulation of keratinocyte differentiation / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / thyroid hormone generation / maintenance of protein location in nucleus / histone H3K4me3 reader activity / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Generic Transcription Pathway / transcription factor TFIIA complex / G protein-coupled receptor internalization / C2H2 zinc finger domain binding

Domain/homology:

Mediator complex subunit 26, C-terminal domain / Mediator complex subunit 26, middle domain / Mediator of RNA polymerase II transcription subunit 26 / Mediator complex subunit 26 C-terminal / Mediator complex subunit 26 middle domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med19, metazoa / Mediator of RNA pol II transcription subunit 19 / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / : / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator complex subunit 16, central helical bridge / Mediator of RNA polymerase II transcription subunit 15 / : / : / ARC105/Med15 mediator subunit N-terminal KIX domain / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / : / : / : / : / : / : / Mediator complex subunit 30 / Mediator complex subunit 28 / Mediator of RNA polymerase II transcription subunit 14, RM2 / Mediator of RNA polymerase II transcription subunit 14, RM8 / Mediator of RNA polymerase II transcription subunit 14, RM6 / Med14 RM domain / Med14 RM domain / Med14 C-terminal domain / Mediator complex, subunit Med23 / Mediator complex subunit 23 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / : / Mediator complex subunit 16, N-terminal / : / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / TAFII28-like protein domain / Transcription initiation factor TFIID subunit 11-like / hTAFII28-like protein conserved region / TAFII55 protein, conserved region / Transcription initiation factor TFIID subunit 7 / TAFII55 protein conserved region / TAFII55 protein conserved region / Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 8 / Transcription factor TFIID complex subunit 8 C-term / Transcription factor TFIID, subunit 8, C-terminal / Transcription initiation factor TFIID component TAF4

Component:

Histone H3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerase subunit / Histone H2B / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB3 / Transcription initiation factor TFIID subunit 4 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 26 / General transcription factor IIF subunit 2 / General transcription and DNA repair factor IIH helicase subunit XPD / General transcription and DNA repair factor IIH helicase/translocase subunit XPB / TATA-box-binding protein / Transcription initiation factor TFIID subunit 1 / General transcription factor IIE subunit 1 / Transcription initiation factor IIE subunit beta / General transcription factor IIH subunit 1 / General transcription factor IIF subunit 1 / Transcription initiation factor TFIID subunit 6 / Cyclin-dependent kinase 7 / Cyclin-H / CDK-activating kinase assembly factor MAT1 / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / DNA-directed RNA polymerase II subunit RPB9 / Histone H4 / Transcription initiation factor IIB / Transcription initiation factor TFIID subunit 10 / Mediator of RNA polymerase II transcription subunit 21 / General transcription factor IIH subunit 2 / General transcription factor IIH subunit 3 / Mediator of RNA polymerase II transcription subunit 22 / Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID subunit 13 / Transcription initiation factor TFIID subunit 11 / Transcription initiation factor TFIID subunit 7 / Mediator of RNA polymerase II transcription subunit 1 / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 3 / Transcription initiation factor TFIID subunit 2 / Mediator of RNA polymerase II transcription subunit 27 / General transcription factor IIH subunit 5 / Mediator of RNA polymerase II transcription subunit 25 / Transcription initiation factor TFIID subunit 8 / General transcription factor IIH subunit 4 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 31

• Biological species: Homo sapiens (human) / synthetic construct (others) / Xenopus laevis (African clawed frog) / Sus scrofa (pig)
• Method: single particle reconstruction / cryo EM / Resolution: 4.16 Å
• Authors: Chen X / Wang X / Liu W / Ren Y / Qu X / Li J / Yin X

Funding support

1 items

Organization	Grant number	Country
Not funded

• Citation: Journal: Science / Year: 2022; Title: Structures of +1 nucleosome-bound PIC-Mediator complex.; Authors: Xizi Chen / Xinxin Wang / Weida Liu / Yulei Ren / Xuechun Qu / Jiabei Li / Xiaotong Yin / Yanhui Xu / ; Abstract: RNA polymerase II-mediated eukaryotic transcription starts with the assembly of the preinitiation complex (PIC) on core promoters. The +1 nucleosome is well positioned about 40 base pairs downstream of the transcription start site (TSS) and is commonly known as a barrier of transcription. The +1 nucleosome-bound PIC-Mediator structures show that PIC-Mediator prefers binding to T40N nucleosome located 40 base pairs downstream of TSS and contacts T50N but not the T70N nucleosome. The nucleosome facilitates the organization of PIC-Mediator on the promoter by binding TFIIH subunit p52 and Mediator subunits MED19 and MED26 and may contribute to transcription initiation. PIC-Mediator exhibits multiple nucleosome-binding patterns, supporting a structural role of the +1 nucleosome in the coordination of PIC-Mediator assembly. Our study reveals the molecular mechanism of PIC-Mediator organization on chromatin and underscores the significance of the +1 nucleosome in regulating transcription initiation.

History

Deposition	Sep 21, 2022	-
Header (metadata) release	Nov 2, 2022	-
Map release	Nov 2, 2022	-
Update	Nov 6, 2024	-
Current status	Nov 6, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_34360.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: PIC-Mediator in complex with plus 1 nucleosome (T40N) in H-binding state
• Voxel size: X=Y=Z: 1.334 Å

Density

Contour Level	By AUTHOR: 0.205
Minimum - Maximum	-1.2771548 - 2.762073
Average (Standard dev.)	0.014527164 (±0.07546102)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 560.27997 Å α=β=γ: 90.0 °

Supplemental data

Additional map: #1

• File: emd_34360_additional_1.map

Half map: Nucleosome of the PIC-Mediator in complex with plus...

• File: emd_34360_half_map_1.map
• Annotation: Nucleosome of the PIC-Mediator in complex with plus 1 nucleosome (T40N) in H-binding state

Half map: #1

• File: emd_34360_half_map_2.map

Sample components

Entire : PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state

• Entire: Name: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state

Components

• Complex: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
  • DNA: DNA (228-mer)
  • DNA: DNA (228-mer)
  • DNA: DNA (162-mer)
  • DNA: DNA (162-mer)
  • Protein or peptide: Transcription initiation factor IIB
  • Protein or peptide: Transcription initiation factor TFIID subunit 1
  • Protein or peptide: General transcription factor IIE subunit 1
  • Protein or peptide: General transcription factor IIF subunit 1
  • Protein or peptide: General transcription and DNA repair factor IIH helicase subunit XPD
  • Protein or peptide: Histone H3
  • Protein or peptide: DNA-directed RNA polymerase subunit
  • Protein or peptide: Transcription initiation factor TFIID subunit 2
  • Protein or peptide: Transcription initiation factor IIE subunit beta
  • Protein or peptide: General transcription factor IIF subunit 2
  • Protein or peptide: General transcription factor IIH subunit 1
  • Protein or peptide: Histone H4
  • Protein or peptide: DNA-directed RNA polymerase subunit beta
  • Protein or peptide: General transcription factor IIH subunit 4
  • Protein or peptide: Transcription initiation factor IIA subunit 2
  • Protein or peptide: TATA-box-binding protein
  • Protein or peptide: Transcription initiation factor IIA subunit 1
  • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3
  • Protein or peptide: DNA-directed RNA polymerase II subunit E
  • Protein or peptide: DNA-directed RNA polymerase II subunit F
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
  • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5
  • Protein or peptide: RNA_pol_L_2 domain-containing protein
  • Protein or peptide: RPB12
  • Protein or peptide: CDK-activating kinase assembly factor MAT1
  • Protein or peptide: General transcription factor IIH subunit 2
  • Protein or peptide: General transcription factor IIH subunit 3
  • Protein or peptide: General transcription factor IIH subunit 5
  • Protein or peptide: General transcription and DNA repair factor IIH helicase subunit XPB
  • Protein or peptide: Transcription initiation factor TFIID subunit 4
  • Protein or peptide: Transcription initiation factor TFIID subunit 5
  • Protein or peptide: Transcription initiation factor TFIID subunit 6
  • Protein or peptide: Transcription initiation factor TFIID subunit 7
  • Protein or peptide: Transcription initiation factor TFIID subunit 8
  • Protein or peptide: Transcription initiation factor TFIID subunit 9
  • Protein or peptide: Transcription initiation factor TFIID subunit 12
  • Protein or peptide: Transcription initiation factor TFIID subunit 3
  • Protein or peptide: Transcription initiation factor TFIID subunit 10
  • Protein or peptide: Transcription initiation factor TFIID subunit 11
  • Protein or peptide: Transcription initiation factor TFIID subunit 13
  • Protein or peptide: Cyclin-dependent kinase 7
  • Protein or peptide: Cyclin-H
  • Protein or peptide: DNA-directed RNA polymerase II subunit RPB4
  • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 26
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 29
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 27
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 28
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 30
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
  • Protein or peptide: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
  • Protein or peptide: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 23
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 24
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 25
  • Protein or peptide: HISTONE H2A.Z
  • Protein or peptide: Histone H2B
• Ligand: ZINC ION
• Ligand: IRON/SULFUR CLUSTER
• Ligand: MAGNESIUM ION

Supramolecule #1: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state

• Supramolecule: Name: PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#77
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: DNA (228-mer)

• Macromolecule: Name: DNA (228-mer) / type: dna / ID: 1 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 70.328703 KDa

Sequence

String:

(DG)(DA)(DA)(DG)(DG)(DG)(DC)(DG)(DC)(DC) (DT)(DA)(DT)(DA)(DA)(DA)(DA)(DG)(DG)(DG) (DG)(DG)(DT)(DG)(DG)(DG)(DG)(DG)(DC) (DG)(DC)(DG)(DT)(DT)(DC)(DG)(DT)(DC)(DC) (DG) (DC)(DA)(DC)(DG)(DG)(DC)(DC)(DG) (DA)(DT)(DC)(DG)(DA)(DA)(DC)(DA)(DC)(DT) (DC)(DG) (DA)(DG)(DC)(DC)(DG)(DA)(DG) (DC)(DA)(DG)(DA)(DC)(DG)(DT)(DG)(DC)(DC) (DT)(DA)(DC) (DG)(DC)(DT)(DG)(DG)(DA) (DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT) (DG)(DC)(DC)(DG) (DA)(DG)(DG)(DC)(DC) (DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG) (DT)(DC)(DG)(DT)(DA) (DG)(DA)(DC)(DA) (DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC) (DC)(DG)(DC)(DT)(DT)(DA) (DA)(DA)(DC) (DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC) (DG)(DC)(DT)(DG)(DT)(DC)(DC) (DC)(DC) (DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA) (DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG) (DG) (DG)(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC) (DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC) (DA)(DG)(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT) (DC)(DA)(DC)(DA)(DT)(DA)(DT)(DA)(DT)(DA) (DC)(DA)(DT)(DC)(DC)(DT)(DG)(DT)

Macromolecule #2: DNA (228-mer)

• Macromolecule: Name: DNA (228-mer) / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 70.483766 KDa

Sequence

String:

(DA)(DC)(DA)(DG)(DG)(DA)(DT)(DG)(DT)(DA) (DT)(DA)(DT)(DA)(DT)(DG)(DT)(DG)(DA)(DC) (DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG) (DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG) (DA) (DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC) (DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT) (DA)(DA) (DA)(DA)(DC)(DG)(DC)(DG)(DG) (DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC) (DG)(DT)(DA) (DC)(DG)(DT)(DG)(DC)(DG) (DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT) (DG)(DC)(DT)(DA) (DG)(DA)(DG)(DC)(DT) (DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC) (DA)(DA)(DT)(DT)(DG) (DA)(DG)(DC)(DG) (DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC) (DC)(DG)(DG)(DG)(DA)(DT) (DT)(DC)(DT) (DC)(DC)(DA)(DG)(DC)(DG)(DT)(DA)(DG)(DG) (DC)(DA)(DC)(DG)(DT)(DC)(DT) (DG)(DC) (DT)(DC)(DG)(DG)(DC)(DT)(DC)(DG)(DA)(DG) (DT)(DG)(DT)(DT)(DC)(DG)(DA)(DT) (DC) (DG)(DG)(DC)(DC)(DG)(DT)(DG)(DC)(DG)(DG) (DA)(DC)(DG)(DA)(DA)(DC)(DG)(DC)(DG) (DC)(DC)(DC)(DC)(DC)(DA)(DC)(DC)(DC)(DC) (DC)(DT)(DT)(DT)(DT)(DA)(DT)(DA)(DG)(DG) (DC)(DG)(DC)(DC)(DC)(DT)(DT)(DC)

Macromolecule #3: DNA (162-mer)

• Macromolecule: Name: DNA (162-mer) / type: dna / ID: 3 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 46.802422 KDa

Sequence

String:

(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)(DC)(DC)(DC)(DC)(DC) (DC)(DC)

Macromolecule #4: DNA (162-mer)

• Macromolecule: Name: DNA (162-mer) / type: dna / ID: 4 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 53.286477 KDa

Sequence

String:

(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)(DG)(DG)(DG)(DG)(DG) (DG)(DG)

Macromolecule #5: Transcription initiation factor IIB

• Macromolecule: Name: Transcription initiation factor IIB / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO / EC number: histone acetyltransferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.877949 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTG AASFDEFGNS KYQNRRTMSS SDRAMMNAFK EITTMADRIN LPRNIVDRTN NLFKQVYEQK SLKGRANDAI A SACLYIAC RQEGVPRTFK EICAVSRISK KEIGRCFKLI LKALETSVDL ITTGDFMSRF CSNLCLPKQV QMAATHIARK AV ELDLVPG RSPISVAAAA IYMASQASAE KRTQKEIGDI AGVADVTIRQ SYRLIYPRAP DLFPTDFKFD TPVDKLPQL

UniProtKB: Transcription initiation factor IIB

Macromolecule #6: Transcription initiation factor TFIID subunit 1

• Macromolecule: Name: Transcription initiation factor TFIID subunit 1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO / EC number: histone acetyltransferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 212.956172 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MGPGCDLLLR TAATITAAAI MSDTDSDEDS AGGGPFSLAG FLFGNINGAG QLEGESVLDD ECKKHLAGLG ALGLGSLITE LTANEELTG TDGALVNDEG WVRSTEDAVD YSDINEVAED ESRRYQQTMG SLQPLCHSDY DEDDYDADCE DIDCKLMPPP P PPPGPMKK DKDQDSITGE KVDFSSSSDS ESEMGPQEAT QAESEDGKLT LPLAGIMQHD ATKLLPSVTE LFPEFRPGKV LR FLRLFGP GKNVPSVWRS ARRKRKKKHR ELIQEEQIQE VECSVESEVS QKSLWNYDYA PPPPPEQCLS DDEITMMAPV ESK FSQSTG DIDKVTDTKP RVAEWRYGPA RLWYDMLGVP EDGSGFDYGF KLRKTEHEPV IKSRMIEEFR KLEENNGTDL LADE NFLMV TQLHWEDDII WDGEDVKHKG TKPQRASLAG WLPSSMTRNA MAYNVQQGFA ATLDDDKPWY SIFPIDNEDL VYGRW EDNI IWDAQAMPRL LEPPVLTLDP NDENLILEIP DEKEEATSNS PSKESKKESS LKKSRILLGK TGVIKEEPQQ NMSQPE VKD PWNLSNDEYY YPKQQGLRGT FGGNIIQHSI PAVELRQPFF PTHMGPIKLR QFHRPPLKKY SFGALSQPGP HSVQPLL KH IKKKAKMREQ ERQASGGGEM FFMRTPQDLT GKDGDLILAE YSEENGPLMM QVGMATKIKN YYKRKPGKDP GAPDCKYG E TVYCHTSPFL GSLHPGQLLQ AFENNLFRAP IYLHKMPETD FLIIRTRQGY YIRELVDIFV VGQQCPLFEV PGPNSKRAN THIRDFLQVF IYRLFWKSKD RPRRIRMEDI KKAFPSHSES SIRKRLKLCA DFKRTGMDSN WWVLKSDFRL PTEEEIRAMV SPEQCCAYY SMIAAEQRLK DAGYGEKSFF APEEENEEDF QMKIDDEVRT APWNTTRAFI AAMKGKCLLE VTGVADPTGC G EGFSYVKI PNKPTQQKDD KEPQPVKKTV TGTDADLRRL SLKNAKQLLR KFGVPEEEIK KLSRWEVIDV VRTMSTEQAR SG EGPMSKF ARGSRFSVAE HQERYKEECQ RIFDLQNKVL SSTEVLSTDT DSSSAEDSDF EEMGKNIENM LQNKKTSSQL SRE REEQER KELQRMLLAA GSAASGNNHR DDDTASVTSL NSSATGRCLK IYRTFRDEEG KEYVRCETVR KPAVIDAYVR IRTT KDEEF IRKFALFDEQ HREEMRKERR RIQEQLRRLK RNQEKEKLKG PPEKKPKKMK ERPDLKLKCG ACGAIGHMRT NKFCP LYYQ TNAPPSNPVA MTEEQEEELE KTVIHNDNEE LIKVEGTKIV LGKQLIESAD EVRRKSLVLK FPKQQLPPKK KRRVGT TVH CDYLNRPHKS IHRRRTDPMV TLSSILESII NDMRDLPNTY PFHTPVNAKV VKDYYKIITR PMDLQTLREN VRKRLYP SR EEFREHLELI VKNSATYNGP KHSLTQISQS MLDLCDEKLK EKEDKLARLE KAINPLLDDD DQVAFSFILD NIVTQKMM A VPDSWPFHHP VNKKFVPDYY KVIVNPMDLE TIRKNISKHK YQSRESFLDD VNLILANSVK YNGPESQYTK TAQEIVNVC YQTLTEYDEH LTQLEKDICT AKEAALEEAE LESLDPMTPG PYTPQPPDLY DTNTSLSMSR DASVFQDESN MSVLDIPSAT PEKQVTQEG EDGDGDLADE EEGTVQQPQA SVLYEDLLMS EGEDDEEDAG SDEEGDNPFS AIQLSESGSD SDVGSGGIRP K QPRMLQEN TRMDMENEES MMSYEGDGGE ASHGLEDSNI SYGSYEEPDP KSNTQDTSFS SIGGYEVSEE EEDEEEEEQR SG PSVLSQV HLSEDEEDSE DFHSIAGDSD LDSDE

UniProtKB: Transcription initiation factor TFIID subunit 1

Macromolecule #7: General transcription factor IIE subunit 1

• Macromolecule: Name: General transcription factor IIE subunit 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.516094 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRH NYYFINYRTL VNVVKYKLDH MRRRIETDER DSTNRASFKC PVCSSTFTDL EANQLFDPMT GTFRCTFCHT E VEEDESAM PKKDARTLLA RFNEQIEPIY ALLRETEDVN LAYEILEPEP TEIPALKQSK DHAATTAGAA SLAGGHHREA WA TKGPSYE DLYTQNVVIN MDDQEDLHRA SLEGKSAKER PIWLRESTVQ GAYGSEDMKE GGIDMDAFQE REEGHAGPDD NEE VMRALL IHEKKTSSAM AGSVGAAAPV TAANGSDSES ETSESDDDSP PRPAAVAVHK REEDEEEDDE FEEVADDPIV MVAG RPFSY SEVSQRPELV AQMTPEEKEA YIAMGQRMFE DLFE

UniProtKB: General transcription factor IIE subunit 1

Macromolecule #8: General transcription factor IIF subunit 1

• Macromolecule: Name: General transcription factor IIF subunit 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 58.343578 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD LSNKKIYQEE EMPESGAGSE FNRKLREEAR RKKYGIVLK EFRPEDQPWL LRVNGKSGRK FKGIKKGGVT ENTSYYIFTQ CPDGAFEAFP VHNWYNFTPL ARHRTLTAEE A EEEWERRN KVLNHFSIMQ QRRLKDQDQD EDEEEKEKRG RRKASELRIH DLEDDLEMSS DASDASGEEG GRVPKAKKKA PL AKGGRKK KKKKGSDDEA FEDSDDGDFE GQEVDYMSDG SSSSQEEPES KAKAPQQEEG PKGVDEQSDS SEESEEEKPP EED KEEEEE KKAPTPQEKK RRKDSSEESD SSEESDIDSE ASSALFMAKK KTPPKRERKP SGGSSRGNSR PGTPSAEGGS TSST LRAAA SKLEQGKRVS EMPAAKRLRL DTGPQSLSGK STPQPPSGKT TPNSGDVQVT EDAVRRYLTR KPMTTKDLLK KFQTK KTGL SSEQTVNVLA QILKRLNPER KMINDKMHFS LKE

UniProtKB: General transcription factor IIF subunit 1

Macromolecule #9: General transcription and DNA repair factor IIH helicase subunit XPD

• Macromolecule: Name: General transcription and DNA repair factor IIH helicase subunit XPD; type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 87.021078 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MKLNVDGLLV YFPYDYIYPE QFSYMRELKR TLDAKGHGVL EMPSGTGKTV SLLALIMAYQ RAYPLEVTKL IYCSRTVPEI EKVIEELRK LLNFYEKQEG EKLPFLGLAL SSRKNLCIHP EVTPLRFGKD VDGKCHSLTA SYVRAQYQHD TSLPHCRFYE E FDAHGREV PLPAGIYNLD DLKALGRRQG WCPYFLARYS ILHANVVVYS YHYLLDPKIA DLVSKELARK AVVVFDEAHN ID NVCIDSM SVNLTRRTLD RCQGNLETLQ KTVLRIKETD EQRLRDEYRR LVEGLREASA ARETDAHLAN PVLPDEVLQE AVP GSIRTA EHFLGFLRRL LEYVKWRLRV QHVVQESPPA FLSGLAQRVC IQRKPLRFCA ERLRSLLHTL EITDLADFSP LTLL ANFAT LVSTYAKGFT IIIEPFDDRT PTIANPILHF SCMDASLAIK PVFERFQSVI ITSGTLSPLD IYPKILDFHP VTMAT FTMT LARVCLCPMI IGRGNDQVAI SSKFETREDI AVIRNYGNLL LEMSAVVPDG IVAFFTSYQY MESTVASWYE QGILEN IQR NKLLFIETQD GAETSVALEK YQEACENGRG AILLSVARGK VSEGIDFVHH YGRAVIMFGV PYVYTQSRIL KARLEYL RD QFQIRENDFL TFDAMRHAAQ CVGRAIRGKT DYGLMVFADK RFARGDKRGK LPRWIQEHLT DANLNLTVDE GVQVAKYF L RQMAQPFHRE DQLGLSLLSL EQLESEETLK RIEQIAQQL

UniProtKB: General transcription and DNA repair factor IIH helicase subunit XPD

Macromolecule #10: Histone H3

• Macromolecule: Name: Histone H3 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Xenopus laevis (African clawed frog)
• Molecular weight: Theoretical: 15.435126 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSA VMALQEASEA YLVALFEDTN LCAIHAKRVT IMPKDIQLAR RIRGERA

UniProtKB: Histone H3

Macromolecule #11: DNA-directed RNA polymerase subunit

• Macromolecule: Name: DNA-directed RNA polymerase subunit / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 217.450078 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ PEGDEDLTKE KGHGGCGRYQ PRIRRSGLEL YAEWKHVNED SQEKKILLSP ERVHEIFKRI SDEECFVLGM EP RYARPEW MIVTVLPVPP LSVRPAVVMQ GSARNQDDLT HKLADIVKIN NQLRRNEQNG AAAHVIAEDV KLLQFHVATM VDN ELPGLP RAMQKSGRPL KSLKQRLKGK EGRVRGNLMG KRVDFSARTV ITPDPNLSID QVGVPRSIAA NMTFAEIVTP FNID RLQEL VRRGNSQYPG AKYIIRDNGD RIDLRFHPKP SDLHLQTGYK VERHMCDGDI VIFNRQPTLH KMSMMGHRVR ILPWS TFRL NLSVTTPYNA DFDGDEMNLH LPQSLETRAE IQELAMVPRM IVTPQSNRPV MGIVQDTLTA VRKFTKRDVF LERGEV MNL LMFLSTWDGK VPQPAILKPR PLWTGKQIFS LIIPGHINCI RTHSTHPDDE DSGPYKHISP GDTKVVVENG ELIMGIL CK KSLGTSAGSL VHISYLEMGH DITRLFYSNI QTVINNWLLI EGHTIGIGDS IADSKTYQDI QNTIKKAKQD VIEVIEKA H NNELEPTPGN TLRQTFENQV NRILNDARDK TGSSAQKSLS EYNNFKSMVV SGAKGSKINI SQVIAVVGQQ NVEGKRIPF GFKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQL RYGEDGLAGE SVEFQNLATL KPSNKAFEKK FRFDYTNERA LRRTLQEDLV KDVLSNAHIQ NELEREFERM R EDREVLRV IFPTGDSKVV LPCNLLRMIW NAQKIFHINP RLPSDLHPIK VVEGVKELSK KLVIVNGDDP LSRQAQENAT LL FNIHLRS TLCSRRMAEE FRLSGEAFDW LLGEIESKFN QAIAHPGEMV GALAAQSLGE PATQMTLNTF HYAGVSAKNV TLG VPRLKE LINISKKPKT PSLTVFLLGQ SARDAERAKD ILCRLEHTTL RKVTANTAIY YDPNPQSTVV AEDQEWVNVY YEMP DFDVA RISPWLLRVE LDRKHMTDRK LTMEQIAEKI NAGFGDDLNC IFNDDNAEKL VLRIRIMNSD ENKMQEEEEV VDKMD DDVF LRCIESNMLT DMTLQGIEQI SKVYMHLPQT DNKKKIIITE DGEFKALQEW ILETDGVSLM RVLSEKDVDP VRTTSN DIV EIFTVLGIEA VRKALERELY HVISFDGSYV NYRHLALLCD TMTCRGHLMA ITRHGVNRQD TGPLMKCSFE ETVDVLM EA AAHGESDPMK GVSENIMLGQ LAPAGTGCFD LLLDAEKCKY GMEIPTNIPG LGAAGPTGMF FGSAPSPMGG ISPAMTPW N QGATPAYGAW SPSVGSGMTP GAAGFSPSAA SDASGFSPGY SPAWSPTPGS PGSPGPSSPY IPSPGGAMSP SYSPTSPAY EPRSPGGYTP QSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPNYS PTSPNYTPTS P SYSPTSPS YSPTSPNYTP TSPNYSPTSP SYSPTSPSYS PTSPSYSPSS PRYTPQSPTY TPSSPSYSPS SPSYSPTSPK YT PTSPSYS PSSPEYTPTS PKYSPTSPKY SPTSPKYSPT SPTYSPTTPK YSPTSPTYSP TSPVYTPTSP KYSPTSPTYS PTS PKYSPT SPTYSPTSPK GSTYSPTSPG YSPTSPTYSL TSPAISPDDS DEEN

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #12: Transcription initiation factor TFIID subunit 2

• Macromolecule: Name: Transcription initiation factor TFIID subunit 2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 137.159984 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDP TLEVCHSESK QRNLNYFSNA YAAAVSAVDP DAGNGELCIK VPSELWKHVD ELKVLKIHIN FSLDQPKGGL H FVVPSVEG SMAERGAHVF SCGYQNSTRF WFPCVDSYSE LCTWKLEFTV DAAMVAVSNG DLVETVYTHD MRKKTFHYML TI PTAASNI SLAIGPFEIL VDPYMHEVTH FCLPQLLPLL KHTTSYLHEV FEFYEEILTC RYPYSCFKTV FIDEAYVEVA AYA SMSIFS TNLLHSAMII DETPLTRRCL AQSLAQQFFG CFISRMSWSD EWVLKGISGY IYGLWMKKTF GVNEYRHWIK EELD KIVAY ELKTGGVLLH PIFGGGKEKD NPASHLHFSI KHPHTLSWEY YSMFQCKAHL VMRLIENRIS MEFMLQVFNK LLSLA STAS SQKFQSHMWS QMLVSTSGFL KSISNVSGKD IQPLIKQWVD QSGVVKFYGS FAFNRKRNVL ELEIKQDYTS PGTQKY VGP LKVTVQELDG SFNHTLQIEE NSLKHDIPCH SKSRRNKKKK IPLMNGEEVD MDLSAMDADS PLLWIRIDPD MSVLRKV EF EQADFMWQYQ LRYERDVVAQ QESILALEKF PTPASRLALT DILEQEQCFY RVRMSACFCL AKIANSMVST WTGPPAMK S LFTRMFCCKS CPNIVKTNNF MSFQSYFLQK TMPVAMALLR DVHNLCPKEV LTFILDLIKY NDNRKNKFSD NYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD PALFKSYAEY GHFVDIRIA ALEAVVDYTK VDRSYEELQW LLNMIQNDPV PYVRHKILNM LTKNPPFTKN MESPLCNEAL VDQLWKLMNS G TSHDWRLR CGAVDLYFTL FGLSRPSCLP LPELGLVLNL KEKKAVLNPT IIPESVAGNQ EAANNPSSHP QLVGFQNPFS SS QDEEEID MDTVHDSQAF ISHHLNMLER PSTPGLSKYR PASSRSALIP QHSAGCDSTP TTKPQWSLEL ARKGTGKEQA PLE MSMHPA ASAPLSVFTK ESTASKHSDH HHHHHHEHKK KKKKHKHKHK HKHKHDSKEK DKEPFTFSSP ASGRSIRSPS LSD

UniProtKB: Transcription initiation factor TFIID subunit 2

Macromolecule #13: Transcription initiation factor IIE subunit beta

• Macromolecule: Name: Transcription initiation factor IIE subunit beta / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 33.106824 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRH QRGDTHPLTL DEILDETQHL DIGLKQKQWL MTEALVNNPK IEVIDGKYAF KPKYNVRDKK ALLRLLDQHD Q RGLGGILL EDIEEALPNS QKAVKALGDQ ILFVNRPDKK KILFFNDKSC QFSVDEEFQK LWRSVTVDSM DEEKIEEYLK RQ GISSMQE SGPKKVAPIQ RRKKPASQKK RRFKTHNEHL AGVLKDYSDI TSSK

UniProtKB: Transcription initiation factor IIE subunit beta

Macromolecule #14: General transcription factor IIF subunit 2

• Macromolecule: Name: General transcription factor IIF subunit 2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.427309 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVG GQTLTVFTES SSDKLSLEGI VVQRAECRPA ASENYMRLKR LQIEESSKPV RLSQQLDKVV TTNYKPVANH Q YNIEYERK KKEDGKRARA DKQHVLDMLF SAFEKHQYYN LKDLVDITKQ PVVYLKEILK EIGVQNVKGI HKNTWELKPE YR HYQGEEK SD

UniProtKB: General transcription factor IIF subunit 2

Macromolecule #15: General transcription factor IIH subunit 1

• Macromolecule: Name: General transcription factor IIH subunit 1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 62.116492 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MATSSEEVLL IVKKVRQKKQ DGALYLMAER IAWAPEGKDR FTISHMYADI KCQKISPEGK AKIQLQLVLH AGDTTNFHFS NESTAVKER DAVKDLLQQL LPKFKRKANK ELEEKNRMLQ EDPVLFQLYK DLVVSQVISA EEFWANRLNV NATDSSSTSN H KQDVGISA AFLADVRPQT DGCNGLRYNL TSDIIESIFR TYPAVKMKYA ENVPHNMTEK EFWTRFFQSH YFHRDRLNTG SK DLFAECA KIDEKGLKTM VSLGVKNPLL DLTALEDKPL DEGYGISSVP SASNSKSIKE NSNAAIIKRF NHHSAMVLAA GLR KQEAQN EQTSEPSNMD GNSGDADCFQ PAVKRAKLQE SIEYEDLGKN NSVKTIALNL KKSDRYYHGP TPIQSLQYAT SQDI INSFQ SIRQEMEAYT PKLTQVLSSS AASSTITALS PGGALMQGGT QQAINQMVPN DIQSELKHLY VAVGELLRHF WSCFP VNTP FLEEKVVKMK SNLERFQVTK LCPFQEKIRR QYLSTNLVSH IEEMLQTAYN KLHTWQSRRL MKKT

UniProtKB: General transcription factor IIH subunit 1

Macromolecule #16: Histone H4

• Macromolecule: Name: Histone H4 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Xenopus laevis (African clawed frog)
• Molecular weight: Theoretical: 11.394426 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVY ALKRQGRTLY GFGG

UniProtKB: Histone H4

Macromolecule #17: DNA-directed RNA polymerase subunit beta

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 134.041422 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD RDLCELNECP LDPGGYFIIN GSEKVLIAQE KMATNTVYVF AKKDSKYAYT GECRSCLENS SRPTSTIWVS ML ARGGQGA KKSAIGQRIV ATLPYIKQEV PIIIVFRALG FVSDRDILEH IIYDFEDPEM MEMVKPSLDE AFVIQEQNVA LNF IGSRGA KPGVTKEKRI KYAKEVLQKE MLPHVGVSDF CETKKAYFLG YMVHRLLLAA LGRRELDDRD HYGNKRLDLA GPLL AFLFR GMFKNLLKEV RIYAQKFIDR GKDFNLELAI KTRIISDGLK YSLATGNWGD QKKAHQARAG VSQVLNRLTF ASTLS HLRR LNSPIGRDGK LAKPRQLHNT LWGMVCPAET PEGHAVGLVK NLALMAYISV GSQPSPILEF LEEWSMENLE EISPAA IAD ATKIFVNGCW VGIHKDPEQL MNTLRKLRRQ MDIIVSEVSM IRDIREREIR IYTDAGRICR PLLIVEKQKL LLKKRHI DQ LKEREYNNYS WQDLVASGVV EYIDTLEEET VMLAMTPDDL QEKEVAYCST YTHCEIHPSM ILGVCASIIP FPDHNQSP R NTYQSAMGKQ AMGVYITNFH VRMDTLAHVL YYPQKPLVTT RSMEYLRFRE LPAGINSIVA IASYTGYNQE DSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL EGTNRRYTK RDCSTFLRTS ETGIVDQVMV TLNQEGYKFC KIRVRSVRIP QIGDKFASRH GQKGTCGIQY RQEDMPFTCE G ITPDIIIN PHAIPSRMTI GHLIECLQGK VSANKGEIGD ATPFNDAVNV QKISNLLSDY GYHLRGNEVL YNGFTGRKIT SQ IFIGPTY YQRLKHMVDD KIHSRARGPI QILNRQPMEG RSRDGGLRFG EMERDCQIAH GAAQFLRERL FEASDPYQVH VCN LCGIMA IANTRTHTYE CRGCRNKTQI SLVRMPYACK LLFQELMSMS IAPRMMSV

UniProtKB: DNA-directed RNA polymerase subunit beta

Macromolecule #18: General transcription factor IIH subunit 4

• Macromolecule: Name: General transcription factor IIH subunit 4 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.245156 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MESTPSRGLN RVHLQCRNLQ EFLGGLSPGV LDRLYGHPAT CLAVFRELPS LAKNWVMRML FLEQPLPQAA VALWVKKEFS KAQEESTGL LSGLRIWHTQ LLPGGLQGLI LNPIFRQNLR IALLGGGKAW SDDTSQLGPD KHARDVPSLD KYAEERWEVV L HFMVGSPS AAVSQDLAQL LSQAGLMKST EPGEPPCITS AGFQFLLLDT PAQLWYFMLQ YLQTAQSRGM DLVEILSFLF QL SFSTLGK DYSVEGMSDS LLNFLQHLRE FGLVFQRKRK SRRYYPTRLA INLSSGVSGA GGTVHQPGFI VVETNYRLYA YTE SELQIA LIALFSEMLY RFPNMVVAQV TRESVQQAIA SGITAQQIIH FLRTRAHPVM LKQTPVLPPT ITDQIRLWEL ERDR LRFTE GVLYNQFLSQ VDFELLLAHA RELGVLVFEN SAKRLMVVTP AGHSDVKRFW KRQKHSS

UniProtKB: General transcription factor IIH subunit 4

Macromolecule #19: Transcription initiation factor IIA subunit 2

• Macromolecule: Name: Transcription initiation factor IIA subunit 2 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.469091 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG SLNTYRFCDN VWTFVLNDVE FREVTELIK VDKVKIVACD GKNTGSNTTE

UniProtKB: Transcription initiation factor IIA subunit 2

Macromolecule #20: TATA-box-binding protein

• Macromolecule: Name: TATA-box-binding protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.729938 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQ QQQQQQAVAA AAVQQSTSQQ ATQGTSGQAP QLFHSQTLTT APLPGTTPLY PSPMTPMTPI TPATPASESS G IVPQLQNI VSTVNLGCKL DLKTIALRAR NAEYNPKRFA AVIMRIREPR TTALIFSSGK MVCTGAKSEE QSRLAARKYA RV VQKLGFP AKFLDFKIQN MVGSCDVKFP IRLEGLVLTH QQFSSYEPEL FPGLIYRMIK PRIVLLIFVS GKVVLTGAKV RAE IYEAFE NIYPILKGFR KTT

UniProtKB: TATA-box-binding protein

Macromolecule #21: Transcription initiation factor IIA subunit 1

• Macromolecule: Name: Transcription initiation factor IIA subunit 1 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 41.544551 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQ AQPQQTVPQQ AQTQQVLIPA SQQATAPQVI VPDSKLIQHM NASNMSAAAT AATLALPAGV TPVQQILTNS G QLLQVVRA ANGAQYIFQP QQSVVLQQQV IPQMQPGGVQ APVIQQVLAP LPGGISPQTG VIIQPQQILF TGNKTQVIPT TV AAPTPAQ AQITATGQQQ PQAQPAQTQA PLVLQVDGTG DTSSEEDEDE EEDYDDDEEE DKEKDGAEDG QVEEEPLNSE DDV SDEEGQ ELFDTENVVV CQYDKIHRSK NKWKFHLKDG IMNLNGRDYI FSKAIGDAEW

UniProtKB: Transcription initiation factor IIA subunit 1

Macromolecule #22: DNA-directed RNA polymerase II subunit RPB3

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 31.439074 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG FGKEHAKWNP TAGVAFEYDP DNALRHTVYP KPEEWPKSEY SELDEDESQA PYDPNGKPER FYYNVESCGS LR PETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

Macromolecule #23: DNA-directed RNA polymerase II subunit E

• Macromolecule: Name: DNA-directed RNA polymerase II subunit E / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 24.644318 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Macromolecule #24: DNA-directed RNA polymerase II subunit F

• Macromolecule: Name: DNA-directed RNA polymerase II subunit F / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 14.477001 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIISD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

Macromolecule #25: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 17.162273 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #26: DNA-directed RNA polymerase II subunit RPB9

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 14.541221 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKC GHKEAVFFQS HSARAEDAMR LYYVCTAPHC GHRWTE

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

Macromolecule #27: DNA-directed RNA polymerases I, II, and III subunit RPABC5

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 7.655123 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #28: RNA_pol_L_2 domain-containing protein

• Macromolecule: Name: RNA_pol_L_2 domain-containing protein / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 13.310284 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTN AITDLISELS LLEERFRVAI KDKQEGIE

UniProtKB: DNA-directed RNA polymerase II subunit RPB11-a

Macromolecule #29: RPB12

• Macromolecule: Name: RPB12 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 7.018244 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

Macromolecule #30: CDK-activating kinase assembly factor MAT1

• Macromolecule: Name: CDK-activating kinase assembly factor MAT1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.873965 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDF PSLREYNDFL EEVEEIVFNL TNNVDLDNTK KKMEIYQKEN KDVIQKNKLK LTREQEELEE ALEVERQENE Q RRLFIQKE EQLQQILKRK NKQAFLDELE SSDLPVALLL AQHKDRSTQL EMQLEKPKPV KPVTFSTGIK MGQHISLAPI HK LEEALYE YQPLQIETYG PHVPELEMLG RLGYLNHVRA ASPQDLAGGY TSSLACHRAL QDAFSGLFWQ PS

UniProtKB: CDK-activating kinase assembly factor MAT1

Macromolecule #31: General transcription factor IIH subunit 2

• Macromolecule: Name: General transcription factor IIH subunit 2 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.481996 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MDEEPERTKR WEGGYERTWE ILKEDESGSL KATIEDILFK AKRKRVFEHH GQVRLGMMRH LYVVVDGSRT MEDQDLKPNR LTCTLKLLE YFVEEYFDQN PISQIGIIVT KSKRAEKLTE LSGNPRKHIT SLKKAVDMTC HGEPSLYNSL SIAMQTLKHM P GHTSREVL IIFSSLTTCD PSNIYDLIKT LKAAKIRVSV IGLSAEVRVC TVLARETGGT YHVILDESHY KELLTHHVSP PP ASSSSEC SLIRMGFPQH TIASLSDQDA KPSFSMAHLD GNTEPGLTLG GYFCPQCRAK YCELPVECKI CGLTLVSAPH LAR SYHHLF PLDAFQEIPL EEYNGERFCY GCQGELKDQH VYVCAVCQNV FCVDCDVFVH DSLHCCPGCI HKIPAPSGV

UniProtKB: General transcription factor IIH subunit 2

Macromolecule #32: General transcription factor IIH subunit 3

• Macromolecule: Name: General transcription factor IIH subunit 3 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.416008 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF MNRSNKLAVI ASHIQESRFL YPGKNGRLGD FFGDPGNPP EFNPSGSKDG KYELLTSANE VIVEEIKDLM TKSDIKGQHT ETLLAGSLAK ALCYIHRMNK EVKDNQEMKS R ILVIKAAE DSALQYMNFM NVIFAAQKQN ILIDACVLDS DSGLLQQACD ITGGLYLKVP QMPSLLQYLL WVFLPDQDQR SQ LILPPPV HVDYRAACFC HRNLIEIGYV CSVCLSIFCN FSPICTTCET AFKISLPPVL KAKKKKLKVS A

UniProtKB: General transcription factor IIH subunit 3

Macromolecule #33: General transcription factor IIH subunit 5

• Macromolecule: Name: General transcription factor IIH subunit 5 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.060362 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MVNVLKGVLI ECDPAMKQFL LYLDESNALG KKFIIQDIDD THVFVIAELV NVLQERVGEL MDQNAFSLTQ K

UniProtKB: General transcription factor IIH subunit 5

Macromolecule #34: General transcription and DNA repair factor IIH helicase subunit XPB

• Macromolecule: Name: General transcription and DNA repair factor IIH helicase subunit XPB; type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 89.404734 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG TKVDEYGAKD YRLQMPLKDD HTSRPLWVAP DGHIFLEAF SPVYKYAQDF LVAIAEPVCR PTHVHEYKLT AYSLYAAVSV GLQTSDITEY LRKLSKTGVP DGIMQFIKLC T VSYGKVKL VLKHNRYFVE SCHPDVIQHL LQDPVIRECR LRNSEGEATE LITETFTSKS AISKTAESSG GPSTSRVTDP QG KSDIPMD LFDFYEQMDK DEEEEEETQT VSFEVKQEMI EELQKRCIHL EYPLLAEYDF RNDSVNPDIN IDLKPTAVLR PYQ EKSLRK MFGNGRARSG VIVLPCGAGK SLVGVTAACT VRKRCLVLGN SAVSVEQWKA QFKMWSTIDD SQICRFTSDA KDKP IGCSV AISTYSMLGH TTKRSWEAER VMEWLKTQEW GLMILDEVHT IPAKMFRRVL TIVQAHCKLG LTATLVREDD KIVDL NFLI GPKLYEANWM ELQNNGYIAK VQCAEVWCPM SPEFYREYVA IKTKKRILLY TMNPNKFRAC QFLIKFHERR NDKIIV FAD NVFALKEYAI RLNKPYIYGP TSQGERMQIL QNFKHNPKIN TIFISKVGDT SFDLPEANVL IQISSHGGSR RQEAQRL GR VLRAKKGMVA EEYNAFFYSL VSQDTQEMAY STKRQRFLVD QGYSFKVITK LAGMEEEDLA FSTKEEQQQL LQKVLAAT D LDAEEEVVAG EFGSRSSQAS RRFGTMSSMS GADDTVYMEY HSSRSKAPSK HVHPLFKRFR K

UniProtKB: General transcription and DNA repair factor IIH helicase/translocase subunit XPB

Macromolecule #35: Transcription initiation factor TFIID subunit 4

• Macromolecule: Name: Transcription initiation factor TFIID subunit 4 / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 110.221883 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAAGSDLLDE VFFNSEVDEK VVSDLVGSLE SQLAASAAHH HHLAPRTPEV RAAAAGALGN HVVSGSPAGA AGAGPAAPAE GAPGAAPEP PPAGRARPGG GGPQRPGPPS PRRPLVPAGP APPAAKLRPP PEGSAGSCAP VPAAAAVAAG PEPAPAGPAK P AGPAALAA RAGPGPGPGP GPGPGPGPGK PAGPGAAQTL NGSAALLNSH HAAAPAVSLV NNGPAALLPL PKPAAPGTVI QT PPFVGAA APPAPAAPSP PAAPAPAAPA AAPPPPPPAP ATLARPPGHP AGPPTAAPAV PPPAAAQNGG SAGAAPAPAP AAG GPAGVS GQPGPGAAAA APAPGVKAES PKRVVQAAPP AAQTLAASGP ASTAASMVIG PTMQGALPSP AAVPPPAPGT PTGL PKGAA GAVTQSLSRT PTATTSGIRA TLTPTVLAPR LPQPPQNPTN IQNFQLPPGM VLVRSENGQL LMIPQQALAQ MQAQA HAQP QTTMAPRPAT PTSAPPVQIS TVQAPGTPII ARQVTPTTII KQVSQAQTTV QPSATLQRSP GVQPQLVLGG AAQTAS LGT ATAVQTGTPQ RTVPGATTTS SAATETMENV KKCKNFLSTL IKLASSGKQS TETAANVKEL VQNLLDGKIE AEDFTSR LY RELNSSPQPY LVPFLKRSLP ALRQLTPDSA AFIQQSQQQP PPPTSQATTA LTAVVLSSSV QRTAGKTAAT VTSALQPP V LSLTQPTQVG VGKQGQPTPL VIQQPPKPGA LIRPPQVTLT QTPMVALRQP HNRIMLTTPQ QIQLNPLQPV PVVKPAVLP GTKALSAVSA QAAAAQKNKL KEPGGGSFRD DDDINDVASM AGVNLSEESA RILATNSELV GTLTRSCKDE TFLLQAPLQR RILEIGKKH GITELHPDVV SYVSHATQQR LQNLVEKISE TAQQKNFSYK DDDRYEQASD VRAQLKFFEQ LDQIEKQRKD E QEREILMR AAKSRSRQED PEQLRLKQKA KEMQQQELAQ MRQRDANLTA LAAIGPRKKR KVDCPGPGSG AEGSGPGSVV PG SSGVGTP RQFTRQRITR VNLRDLIFCL ENERETSHSL LLYKAFLK

UniProtKB: Transcription initiation factor TFIID subunit 4

Macromolecule #36: Transcription initiation factor TFIID subunit 5

• Macromolecule: Name: Transcription initiation factor TFIID subunit 5 / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 86.932109 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAALAEEQTE VAVKLEPEGP PTLLPPQAGD GAGEGSGGTT NNGPNGGGGN VAASSSTGGD GGTPKPTVAV SAAAPAGAAP VPAAAPDAG APHDRQTLLA VLQFLRQSKL REAEEALRRE AGLLEEAVAG SGAPGEVDSA GAEVTSALLS RVTASAPGPA A PDPPGTGA SGATVVSGSA SGPAAPGKVG SVAVEDQPDV SAVLSAYNQQ GDPTMYEEYY SGLKHFIECS LDCHRAELSQ LF YPLFVHM YLELVYNQHE NEAKSFFEKF HGDQECYYQD DLRVLSSLTK KEHMKGNETM LDFRTSKFVL RISRDSYQLL KRH LQEKQN NQIWNIVQEH LYIDIFDGMP RSKQQIDAMV GSLAGEAKRE ANKSKVFFGL LKEPEIEVPL DDEDEEGENE EGKP KKKKP KKDSIGSKSK KQDPNAPPQN RIPLPELKDS DKLDKIMNMK ETTKRVRLGP DCLPSICFYT FLNAYQGLTA VDVTD DSSL IAGGFADSTV RVWSVTPKKL RSVKQASDLS LIDKESDDVL ERIMDEKTAS ELKILYGHSG PVYGASFSPD RNYLLS SSE DGTVRLWSLQ TFTCLVGYKG HNYPVWDTQF SPYGYYFVSG GHDRVARLWA TDHYQPLRIF AGHLADVNCT RFHPNSN YV ATGSADRTVR LWDVLNGNCV RIFTGHKGPI HSLTFSPNGR FLATGATDGR VLLWDIGHGL MVGELKGHTD TVCSLRFS R DGEILASGSM DNTVRLWDAI KAFEDLETDD FTTATGHINL PENSQELLLG TYMTKSTPVV HLHFTRRNLV LAAGAYSPQ

UniProtKB: Transcription initiation factor TFIID subunit 5

Macromolecule #37: Transcription initiation factor TFIID subunit 6

• Macromolecule: Name: Transcription initiation factor TFIID subunit 6 / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 72.749297 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA KPGQEEDGPL KGKGQGATTA DGKGKEKKAP PLLEGAPLRL KPRSIHELSV EQQLYYKEIT EACVGSCEAK RA EALQSIA TDPGLYQMLP RFSTFISEGV RVNVVQNNLA LLIYLMRMVK ALMDNPTLYL EKYVHELIPA VMTCIVSRQL CLR PDVDNH WALRDFAARL VAQICKHFST TTNNIQSRIT KTFTKSWVDE KTPWTTRYGS IAGLAELGHD VIKTLILPRL QQEG ERIRS VLDGPVLSNI DRIGADHVQS LLLKHCAPVL AKLRPPPDNQ DAYRAEFGSL GPLLCSQVVK ARAQAALQAQ QVNRT TLTI TQPRPTLTLS QAPQPGPRTP GLLKVPGSIA LPVQTLVSAR AAAPPQPSPP PTKFIVMSSS SSAPSTQQVL SLSTSA PGS GSTTTSPVTT TVPSVQPIVK LVSTATTAPP STAPSGPGSV QKYIVVSLPP TGEGKGGPTS HPSPVPPPAS SPSPLSG SA LCGGKQEAGD SPPPAPGTPK ANGSQPNSGS PQPAP

UniProtKB: Transcription initiation factor TFIID subunit 6

Macromolecule #38: Transcription initiation factor TFIID subunit 7

• Macromolecule: Name: Transcription initiation factor TFIID subunit 7 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 40.325117 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTA DICQMLVSTV DGDLYPPVEE PVASTDPKAS KKKDKDKEKK FIWNHGITLP LKNVRKRRFR KTAKKKYIES P DVEKEVKR LLSTDAEAVS TRWEIIAEDE TKEAENQGLD ISSPGMSGHR QGHDSLEHDE LREIFNDLSS SSEDEDETQH QD EEDINII DTEEDLERQL QDKLNESDEQ HQENEGTNQL VMGIQKQIDN MKGKLQETQD RAKRQEDLIM KVENLALKNR FQA VLDELK QKEDREKEQL SSLQEELESL LEK

UniProtKB: Transcription initiation factor TFIID subunit 7

Macromolecule #39: Transcription initiation factor TFIID subunit 8

• Macromolecule: Name: Transcription initiation factor TFIID subunit 8 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.304359 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPT LSDIVVTLVE MGFNVDTLPA YAKRSQRMVI TAPPVTNQPV TPKALTAGQN RPHPPHIPSH FPEFPDPHTY I KTPTYREP VSDYQVLREK AASQRRDVER ALTRFMAKTG ETQSLFKDDV STFPLIAARP FTIPYLTALL PSELEMQQME ET DSSEQDE QTDTENLALH ISMEDSGAEK ENTSVLQQNP SLSGSRNGEE NIIDNPYLRP VKKPKIRRKK SLS

UniProtKB: Transcription initiation factor TFIID subunit 8

Macromolecule #40: Transcription initiation factor TFIID subunit 9

• Macromolecule: Name: Transcription initiation factor TFIID subunit 9 / type: protein_or_peptide / ID: 40 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.006838 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPR DFLLDIARQR NQTPLPLIKP YSGPRLPPDR YCLTAPNYRL KSLQKKASTS AGRITVPRLS VGSVTSRPST P TLGTPTPQ TMSVSTKVGT PMSLTGQRFT VQMPTSQSPA VKASIPATSA VQNVLINPSL IGSKNILITT NMMSSQNTAN ES SNALKRK REDDDDDDDD DDDYDNL

UniProtKB: Transcription initiation factor TFIID subunit 9

Macromolecule #41: Transcription initiation factor TFIID subunit 12

• Macromolecule: Name: Transcription initiation factor TFIID subunit 12 / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.948467 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL SPENNQVLTK KKLQDLVREV DPNEQLDEDV EEMLLQIAD DFIESVVTAA CQLARHRKSS TLEVKDVQLH LERQWNMWIP GFGSEEIRPY KKACTTEAHK QRMALIRKTT K K

UniProtKB: Transcription initiation factor TFIID subunit 12

Macromolecule #42: Transcription initiation factor TFIID subunit 3

• Macromolecule: Name: Transcription initiation factor TFIID subunit 3 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 103.76932 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MCESYSRSLL RVSVAQICQA LGWDSVQLSA CHLLTDVLQR YLQQLGRGCH RYSELYGRTD PILDDVGEAF QLMGVSLHEL EDYIHNIEP VTFPHQIPSF PVSKNNVLQF PQPGSKDAEE RKEYIPDYLP PIVSSQEEEE EEQVPTDGGT SAEAMQVPLE E DDELEEEE IINDENFLGK RPLDSPEAEE LPAMKRPRLL STKGDTLDVV LLEAREPLSS INTQKIPPML SPVHVQDSTD LA PPSPEPP MLAPVAKSQM PTAKPLETKS FTPKTKTKTS SPGQKTKSPK TAQSPAMVGS PIRSPKTVSK EKKSPGRSKS PKS PKSPKV TTHIPQTPVR PETPNRTPSA TLSEKISKET IQVKQIQTPP DAGKLNSENQ PKKAVVADKT IEASIDAVIA RACA EREPD PFEFSSGSES EGDIFTSPKR ISGPECTTPK ASTSANNFTK SGSTPLPLSG GTSSSDNSWT MDASIDEVVR KAKLG TPSN MPPNFPYISS PSVSPPTPEP LHKVYEEKTK LPSSVEVKKK LKKELKTKMK KKEKQRDRER EKDKNKDKSK EKDKVK EKE KDKETGRETK YPWKEFLKEE EADPYKFKIK EFEDVDPKVK LKDGLVRKEK EKHKDKKKDR EKGKKDKDKR EKEKVKD KG REDKMKAPAP PLVLPPKELA LPLFSPATAS RVPAMLPSLL PVLPEKLFEE KEKVKEKEKK KDKKEKKKKK EKEKEKKE K EREKEKRERE KREKEKEKHK HEKIKVEPVA LAPSPVIPRL TLRVGAGQDK IVISKVVPAP EAKPAPSQNR PKTPPPAPA PAPGPMLVSP APVPLPLLAQ AAAGPALLPS PGPAASGASA KAPVRSVVTE TVSTYVIRDE WGNQIWICPG CNKPDDGSPM IGCDDCDDW YHWPCVGIMT APPEEMQWFC PKCANKKKDK KHKKRKHRAH

UniProtKB: Transcription initiation factor TFIID subunit 3

Macromolecule #43: Transcription initiation factor TFIID subunit 10

• Macromolecule: Name: Transcription initiation factor TFIID subunit 10 / type: protein_or_peptide / ID: 43 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.731248 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPE GAISNGVYVL PSAANGDVKP VVSSTPLVDF LMQLEDYTPT IPDAVTGYYL NRAGFEASDP RIIRLISLAA Q KFISDIAN DALQHCKMKG TASGSSRSKS KDRKYTLTME DLTPALSEYG INVKKPHYFT

UniProtKB: Transcription initiation factor TFIID subunit 10

Macromolecule #44: Transcription initiation factor TFIID subunit 11

• Macromolecule: Name: Transcription initiation factor TFIID subunit 11 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.340094 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MDDAHESPSD KGGETGESDE TAAVPGDPGA TDTDGIPEET DGDADVDLKE AAAEEGELES QDVSDLTTVE REDSSLLNPA AKKLKIDTK EKKEKKQKVD EDEIQKMQIL VSSFSEEQLN RYEMYRRSAF PKAAIKRLIQ SITGTSVSQN VVIAMSGISK V FVGEVVEE ALDVCEKWGE MPPLQPKHMR EAVRRLKSKG QIPNSKHKKI IFF

UniProtKB: Transcription initiation factor TFIID subunit 11

Macromolecule #45: Transcription initiation factor TFIID subunit 13

• Macromolecule: Name: Transcription initiation factor TFIID subunit 13 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.307068 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MADEEEDPTF EEENEEIGGG AEGGQGKRKR LFSKELRCMM YGFGDDQNPY TESVDILEDL VIEFITEMTH KAMSIGRQGR VQVEDIVFL IRKDPRKFAR VKDLLTMNEE LKRARKAFDE ANYGS

UniProtKB: Transcription initiation factor TFIID subunit 13

Macromolecule #46: Cyclin-dependent kinase 7

• Macromolecule: Name: Cyclin-dependent kinase 7 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO / EC number: cyclin-dependent kinase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.032242 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MALDVKSRAK RYEKLDFLGE GQFATVYKAR DKNTNQIVAI AKIKLGHRSE AKDGINRTAL REIKLLQELS HPNIIGLLDA FGHKSNISL VFDFMETDLE VIIKDNSLVL TPSHIKAYML MTLQGLEYLH QHWILHRDLK PNNLLLDENG VLKLADFGLA K SFGSPNRA YTHQVVTRWY RAPELLFGAR MYGVGVDMWA VGCILAELLL RVPFLPGDSD LDQLTRIFET LGTPTEEQWP DM CSLPDYV TFKSFPGIPL HHIFSAAGDD LLDLIQGLFL FNPCARITAT QALKMKYFSN RPGPTPGCQL PRPNCPVETL KEQ SNPALA IKRKRTEALE QGGLPKKLIF

UniProtKB: Cyclin-dependent kinase 7

Macromolecule #47: Cyclin-H

• Macromolecule: Name: Cyclin-H / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.695473 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKR FYLNNSVMEY HPRIIMLTCA FLACKVDEFN VSSPQFVGNL RESPLGQEKA LEQILEYELL LIQQLNFHLI V HNPYRPFE GFLIDLKTRY PILENPEILR KTADDFLNRI ALTDAYLLYT PSQIALTAIL SSASRAGITM ESYLSESLML KE NRTCLSQ LLDIMKSMRN LVKKYEPPRS EEVAVLKQKL ERCHSAELAL NVITKKRKGY EDDDYVSKKS KHEEEEWTDD DLV ESL

UniProtKB: Cyclin-H

Macromolecule #48: DNA-directed RNA polymerase II subunit RPB4

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB4 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 16.331255 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQ KKLHKFELAC LANLCPETAE ESKALIPSLE GRFEDEELQQ ILDDIQTKRS FQY

UniProtKB: DNA-directed RNA polymerase II subunit RPB4

Macromolecule #49: DNA-directed RNA polymerase II subunit RPB7

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 19.314283 KDa
• Recombinant expression: Organism: Sus scrofa (pig)

Sequence

String:

MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVV TQVNKVGLFT EIGPMSCFIS RHSIPSEMEF DPNSNPPCYK TMDEDIVIQQ DDEIRLKIVG TRVDKNDIFA I GSLMDDYL GLVS

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #50: Mediator of RNA polymerase II transcription subunit 7

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.285281 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINF LDLLDILIRS PGSIKREEKL EDLKLLFVHV HHLINEYRPH QARETLRVMM EVQKRQRLET AERFQKHLER V IEMIQNCL ASLPDDLPHS EAGMRVKTEP MDADDSNNCT GQNEHQRENS GHRRDQIIEK DAALCVLIDE MNERP

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

Macromolecule #51: Mediator of RNA polymerase II transcription subunit 10

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 10; type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.710969 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLNQK LNFIVTGLQD IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERAL AKNEQVKGKI DTMKKFKSLL IQELSKVFPE DMAKYRSIRG EDHPPS

UniProtKB: Mediator of RNA polymerase II transcription subunit 10

Macromolecule #52: Mediator of RNA polymerase II transcription subunit 14

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 14; type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 160.787594 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR LSTLIEFLLH RAYSELMVLT DLLPRKSDVE RKIEIVQFA SRTRQLFVRL LALVKWANNA GKVEKCAMIS SFLDQQAILF VDTADRLASL ARDALVHARL PSFAIPYAID V LTTGSYPR LPTCIRDKII PPDPITKIEK QATLHQLNQI LRHRLVTTDL PPQLANLTVA NGRVKFRVEG EFEATLTVMG DD PDVPWRL LKLEILVEDK ETGDGRALVH SMQISFIHQL VQSRLFADEK PLQDMYNCLH SFCLSLQLEV LHSQTLMLIR ERW GDLVQV ERYHAGKCLS LSVWNQQVLG RKTGTASVHK VTIKIDENDV SKPLQIFHDP PLPASDSKLV ERAMKIDHLS IEKL LIDSV HARAHQKLQE LKAILRGFNA NENSSIETAL PALVVPILEP CGNSECLHIF VDLHSGMFQL MLYGLDQATL DDMEK SVND DMKRIIPWIQ QLKFWLGQQR CKQSIKHLPT ISSETLQLSN YSTHPIGNLS KNKLFIKLTR LPQYYIVVEM LEVPNK PTQ LSYKYYFMSV NAADREDSPA MALLLQQFKE NIQDLVFRTK TGKQTRTNAK RKLSDDPCPV ESKKTKRAGE MCAFNKV LA HFVAMCDTNM PFVGLRLELS NLEIPHQGVQ VEGDGFSHAI RLLKIPPCKG ITEETQKALD RSLLDCTFRL QGRNNRTW V AELVFANCPL NGTSTREQGP SRHVYLTYEN LLSEPVGGRK VVEMFLNDWN SIARLYECVL EFARSLPDIP AHLNIFSEV RVYNYRKLIL CYGTTKGSSI SIQWNSIHQK FHISLGTVGP NSGCSNCHNT ILHQLQEMFN KTPNVVQLLQ VLFDTQAPLN AINKLPTVP MLGLTQRTNT AYQCFSILPQ SSTHIRLAFR NMYCIDIYCR SRGVVAIRDG AYSLFDNSKL VEGFYPAPGL K TFLNMFVD SNQDARRRSV NEDDNPPSPI GGDMMDSLIS QLQPPPQQQP FPKQPGTSGA YPLTSPPTSY HSTVNQSPSM MH TQSPGNL HAASSPSGAL RAPSPASFVP TPPPSSHGIS IGPGASFASP HGTLDPSSPY TMVSPSGRAG NWPGSPQVSG PSP AARMPG MSPANPSLHS PVPDASHSPR AGTSSQTMPT NMPPPRKLPQ RSWAASIPTI LTHSALNILL LPSPTPGLVP GLAG SYLCS PLERFLGSVI MRRHLQRIIQ QETLQLINSN EPGVIMFKTD ALKCRVALSP KTNQTLQLKV TPENAGQWKP DELQV LEKF FETRVAGPPF KANTLIAFTK LLGAPTHILR DCVHIMKLEL FPDQATQLKW NVQFCLTIPP SAPPIAPPGT PAVVLK SKM LFFLQLTQKT SVPPQEPVSI IVPIIYDMAS GTTQQADIPR QQNSSVAAPM MVSNILKRFA EMNPPRQGEC TIFAAVR DL MANLTLPPGG RP

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

Macromolecule #53: Mediator of RNA polymerase II transcription subunit 19

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 19; type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.321146 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MENFTALFGA QADPPPPPTA LGFGPGKPPP PPPPPAGGGP GTAPPPTAAT APPGADKSGA GCGPFYLMRE LPGSTELTGS TNLITHYNL EQAYNKFCGK KVKEKLSNFL PDLPGMIDLP GSHDNSSLRS LIEKPPILSS SFNPITGTML AGFRLHTGPL P EQCRLMHI QPPKKKNKHK HKQSRTQDPV PPETPSDSDH KKKKKKKEED PDRKRKKKEK KKKKNRHSPD HPGMGSSQAS SS SSLR

UniProtKB: Mediator of RNA polymerase II transcription subunit 19

Macromolecule #54: Mediator of RNA polymerase II transcription subunit 21

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 21; type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.575234 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP TEEYAQLFAA LIARTAKDID VLIDSLPSEE STAALQAAS LYKLEEENHE AATCLEDVVY RGDMLLEKIQ SALADIAQSQ LKTRSGTHSQ SLPDS

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

Macromolecule #55: Mediator of RNA polymerase II transcription subunit 1

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 168.697328 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MKAQGETEES EKLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNG LGSHLSASGT ECYITSDMFY VEVQLDPAGQ LCDVKVAHHG ENPVSCPELV QQLREKNFDE FSKHLKGLVN L YNLPGDNK LKTKMYLALQ SLEQDLSKMA IMYWKATNAG PLDKILHGSV GYLTPRSGGH LMNLKYYVSP SDLLDDKTAS PI ILHENNV SRSLGMNASV TIEGTSAVYK LPIAPLIMGS HPVDNKWTPS FSSITSANSV DLPACFFLKF PQPIPVSRAF VQK LQNCTG IPLFETQPTY APLYELITQF ELSKDPDPIP LNHNMRFYAA LPGQQHCYFL NKDAPLPDGR SLQGTLVSKI TFQH PGRVP LILNLIRHQV AYNTLIGSCV KRTILKEDSP GLLQFEVCPL SESRFSVSFQ HPVNDSLVCV VMDVQDSTHV SCKLY KGLS DALICTDDFI AKVVQRCMSI PVTMRAIRRK AETIQADTPA LSLIAETVED MVKKNLPPAS SPGYGMTTGN NPMSGT TTP TNTFPGGPIT TLFNMSMSIK DRHESVGHGE DFSKVSQNPI LTSLLQITGN GGSTIGSSPT PPHHTPPPVS SMAGNTK NH PMLMNLLKDN PAQDFSTLYG SSPLERQNSS SGSPRMEICS GSNKTKKKKS SRLPPEKPKH QTEDDFQREL FSMDVDSQ N PIFDVNMTAD TLDTPHITPA PSQCSTPPTT YPQPVPHPQP SIQRMVRLSS SDSIGPDVTD ILSDIAEEAS KLPSTSDDC PAIGTPLRDS SSSGHSQSTL FDSDVFQTNN NENPYTDPAD LIADAAGSPS SDSPTNHFFH DGVDFNPDLL NSQSQSGFGE EYFDESSQS GDNDDFKGFA SQALNTLGVP MLGGDNGETK FKGNNQADTV DFSIISVAGK ALAPADLMEH HSGSQGPLLT T GDLGKEKT QKRVKEGNGT SNSTLSGPGL DSKPGKRSRT PSNDGKSKDK PPKRKKADTE GKSPSHSSSN RPFTPPTSTG GS KSPGSAG RSQTPPGVAT PPIPKITIQI PKGTVMVGKP SSHSQYTSSG SVSSSGSKSH HSHSSSSSSS ASTSGKMKSS KSE GSSSSK LSSSMYSSQG SSGSSQSKNS SQSGGKPGSS PITKHGLSSG SSSTKMKPQG KPSSLMNPSL SKPNISPSHS RPPG GSDKL ASPMKPVPGT PPSSKAKSPI SSGSGGSHMS GTSSSSGMKS SSGLGSSGSL SQKTPPSSNS CTASSSSFSS SGSSM SSSQ NQHGSSKGKS PSRNKKPSLT AVIDKLKHGV VTSGPGGEDP LDGQMGVSTN SSSHPMSSKH NMSGGEFQGK REKSDK DKS KVSTSGSSVD SSKKTSESKN VGSTGVAKII ISKHDGGSPS IKAKVTLQKP GESSGEGLRP QMASSKNYGS PLISGST PK HERGSPSHSK SPAYTPQNLD SESESGSSIA EKSYQNSPSS DDGIRPLPEY STEKHKKHKK EKKKVKDKDR DRDRDKDR D KKKSHSIKPE SWSKSPISSD QSLSMTSNTI LSADRPSRLS PDFMIGEEDD DLMDVALIGN

UniProtKB: Mediator of RNA polymerase II transcription subunit 1

Macromolecule #56: Mediator of RNA polymerase II transcription subunit 4

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.781424 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKI HHEMQVLEKE VEKRDSDIQQ LQKQLKEAEQ ILATAVYQAK EKLKSIEKAR KGAISSEEII KYAHRISASN A VCAPLTWV PGDPRRPYPT DLEMRSGLLG QMNNPSTNGV NGHLPGDALA AGRLPDVLAP QYPWQSNDMS MNMLPPNHSS DF LLEPPGH NKENEDDVEI MSTDSSSSSS ESD

UniProtKB: Mediator of RNA polymerase II transcription subunit 4

Macromolecule #57: Mediator of RNA polymerase II transcription subunit 6

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.464627 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSP AQVIPLADYY IIAGVIYQAP DLGSVINSRV LTAVHGIQSA FDEAMSYCRY HPSKGYWWHF KDHEEQDKVR P KAKRKEEP SSIFQRQRVD ALLLDLRQKF PPKFVQLKPG EKPVPVDQTK KEAEPIPETV KPEEKETTKN VQQTVSAKGP PE KRMRLQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

Macromolecule #58: Mediator of RNA polymerase II transcription subunit 9

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.42275 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNA LKSKFQEMRK LISTMPGIHL SPEQQQQQLQ SLREQVRTKN ELLQKYKSLC MFEIPKE

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

Macromolecule #59: Mediator of RNA polymerase II transcription subunit 31

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 31; type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.826116 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKD PEYAKYLKYP QCLHMLELLQ YEHFRKELV NAQCAKFIDE QQILHWQHYS RKRMRLQQAL AEQQQQNNTS GK

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

Macromolecule #60: Mediator of RNA polymerase II transcription subunit 17

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 17; type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 72.984047 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MSGVRAVRIS IESACEKQVH EVGLDGTETY LPPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGTEGDAQEW PGAGSSADQD DEEGVVKFQ PSLWPWDSVR NNLRSALTEM CVLYDVLSIV RDKKFMTLDP VSQDALPPKQ NPQTLQLISK KKSLAGAAQI L LKGAERLT KSVTENQENK LQRDFNSELL RLRQHWKLRK VGDKILGDLS YRSAGSLFPH HGTFEVIKNT DLDLDKKIPE DY CPLDVQI PSDLEGSAYI KVSIQKQAPD IGDLGTVNLF KRPLPKSKPG SPHWQTKLEA AQNVLLCKEI FAQLSREAVQ IKS QVPHIV VKNQIISQPF PSLQLSISLC HSSNDKKSQK FATEKQCPED HLYVLEHNLH LLIREFHKQT LSSIMMPHPA SAPF GHKRM RLSGPQAFDK NEINSLQSSE GLLEKIIKQA KHIFLRSRAA ATIDSLASRI EDPQIQAHWS NINDVYESSV KVLIT SQGY EQICKSIQLQ LNIGVEQIRV VHRDGRVITL SYQEQELQDF LLSQMSQHQV HAVQQLAKVM GWQVLSFSNH VGLGPI ESI GNASAITVAS PSGDYAISVR NGPESGSKIM VQFPRNQCKD LPKSDVLQDN KWSHLRGPFK EVQWNKMEGR NFVYKME LL MSALSPCLL

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

Macromolecule #61: Mediator of RNA polymerase II transcription subunit 26

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 26; type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.544438 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE TRLGKLINDV RKKTKNEELA KRAKKLLRSW QKLIEPAHQ HEAALRGLAG ATGSANGGAH NCRPEVGAAG PPRSIHDLKS RNDLQRLPGQ RLDRLGSRKR RGDQRDLGHP G PPPKVSKA SHDPLVPNSS PLPTNGISGS PESFASSLDG SGHAGPEGSR LERDENDKHS GKIPVNAVRP HTSSPGLGKP PG PCLQPKA SVLQQLDRVD ETPGPPHPKG PPRCSFSPRN SRHEGSFARQ QSLYAPKGSV PSPSPRPQAL DATQVPSPLP LAQ PSTPPV RRLELLPSAE SPVCWLEQPE SHQRLAGPGC KAGLSPAEPL LSRAGFSPDS SKADSDAASS GGSDSKKKKR YRPR DYTVN LDGQVAEAGV KPVRLKERKL TFDPMTRQIK PLTQKEPVRA DSPVHMEQQS RTELDKQEAK ASLQSPFEQT NWKEL SRNE IIQSYLSRQS SLLSSSGAQT PGAHHFMSEY LKQEESTRQG ARQLHVLVPQ SPPTDLPGLT REVTQDDLDR IQASQW PGV NGCQDTQGNW YDWTQCISLD PHGDDGRLNI LPYVCLD

UniProtKB: Mediator of RNA polymerase II transcription subunit 26

Macromolecule #62: Mediator of RNA polymerase II transcription subunit 29

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 29; type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.089801 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ QQDFDPVQRY KMLIPQLKES LQTLMKVAAQ NLIQNTNID NGQKSSDGPI QRFDKCLEEF YALCDQLELC LRLAHECLSQ SCDSAKHSPT LVPTATKPDA VQPDSLPYPQ Y LAVIKAQI SCAKDIHTAL LDCANKVTGK TPAPPAGPGG TL

UniProtKB: Mediator of RNA polymerase II transcription subunit 29

Macromolecule #63: Mediator of RNA polymerase II transcription subunit 27

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 27; type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.478742 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNS GLLSLDPVQD KTPLYSQLLQ AYKWSNKLQY HAGLASGLLN QQSLKRSANQ MGVSAKRRPK AQPTTLVLPP Q YVDDVISR IDRMFPEMSI HLSRPNGTSA MLLVTLGKVL KVIVVMRSLF IDRTIVKGYN ENVYTEDGKL DIWSKSNYQV FQ KVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 27

Macromolecule #64: Mediator of RNA polymerase II transcription subunit 28

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 28; type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 19.540107 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIAR QTECFFLQKR LQLSVQKPEQ VIKEDVSELR NELQRKDALV QKHLTKLRHW QQVLEDINVQ HKKPADIPQG S LAYLEQAS ANIPAPLKPT

UniProtKB: Mediator of RNA polymerase II transcription subunit 28

Macromolecule #65: Mediator of RNA polymerase II transcription subunit 30

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 30; type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.301229 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD NLRQLSVLF RKLRLVYDKC NENCGGMDPI PVEQLIPYVE EDGSKNDDRA GPPRFASEER REIAEVNKKL KQKNQQLKQI M DQLRNLIW DINAMLAMRN

UniProtKB: Mediator of RNA polymerase II transcription subunit 30

Macromolecule #66: Mediator of RNA polymerase II transcription subunit 15

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 15; type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 86.830102 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA RLIIHFRDIH NKKSQASVSD PMNALQSLT GGPAAGAAGI GMPPRGPGQS LGGMGSLGAM GQPMSLSGQP PPGTSGMAPH SMAVVSTATP QTQLQLQQVA L QQQQQQQQ FQQQQQAALQ QQQQQQQQQQ FQAQQSAMQQ QFQAVVQQQQ QLQQQQQQQQ HLIKLHHQNQ QQIQQQQQQL QR IAQLQLQ QQQQQQQQQQ QQQQQALQAQ PPIQQPPMQQ PQPPPSQALP QQLQQMHHTQ HHQPPPQPQQ PPVAQNQPSQ LPP QSQTQP LVSQAQALPG QMLYTQPPLK FVRAPMVVQQ PPVQPQVQQQ QTAVQTAQAA QMVAPGVQMI TEALAQGGMH IRAR FPPTT AVSAIPSSSI PLGRQPMAQV SQSSLPMLSS PSPGQQVQTP QSMPPPPQPS PQPGQPSSQP NSNVSSGPAP SPSSF LPSP SPQPSQSPVT ARTPQNFSVP SPGPLNTPVN PSSVMSPAGS SQAEEQQYLD KLKQLSKYIE PLRRMINKID KNEDRK KDL SKMKSLLDIL TDPSKRCPLK TLQKCEIALE KLKNDMAVPT PPPPPVPPTK QQYLCQPLLD AVLANIRSPV FNHSLYR TF VPAMTAIHGP PITAPVVCTR KRRLEDDERQ SIPSVLQGEV ARLDPKFLVN LDPSHCSNNG TVHLICKLDD KDLPSVPP L ELSVPADYPA QSPLWIDRQW QYDANPFLQS VHRCMTSRLL QLPDKHSVTA LLNTWAQSVH QACLSAA

UniProtKB: Mediator of RNA polymerase II transcription subunit 15

Macromolecule #67: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8

• Macromolecule: Name: Isoform 2 of Mediator of RNA polymerase II transcription subunit 8; type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 29.112906 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MQREEKQLEA SLDALLSQVA DLKNSLGSFI CKLENEYGRL TWPSVLDSFA LLSGQLNTLN KVLKHEKTPL FRNQVIIPLV LSPDRDEDL MRQTEGRVPV FSHEVVPDHL RTKPDPEVEE QEKQLTTDAA RIGADAAQKQ IQSLNKMCSN LLEKISKEER E SESGGLRP NKQTFNPTDT NALVAAVAFG KGLSNWRPSG SSGPGQAGQP GAGTILAGTS GLQQVQMAGA PSQQQPMLSG VQ MAQAGQP GKMPSGIKTN IKSASMHPYQ R

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

Macromolecule #68: Mediator of RNA polymerase II transcription subunit 11

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 11; type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.145829 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MATYSLANER LRALEDIERE IGAILQNAGT VILELSKEKT NERLLDRQAA AFTASVQHVE AELSAQIRYL TQVATGQPHE GSSYSSRKD CQMALKRVDY ARLKLSDVAR TCEQMLEN

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

Macromolecule #69: Mediator of RNA polymerase II transcription subunit 18

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 18; type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.69373 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLG QPEMGDKNRH ALVRNCVDIA TSENLTDFLM EMGFRMDHEF VAKGHLFRKG IMKIMVYKIF RILVPGNTDS T EALSLSYL VELSVVAPAG QDMVSDDMKN FAEQLKPLVH LEKIDPKRLM

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

Macromolecule #70: Mediator of RNA polymerase II transcription subunit 20

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 20; type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.243762 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADT NFDVLMVKLK GFFQSAKASK IETRGTRYQY CDFLVKVGTV TMGPSARGIS VEVEYGPCVV ASDCWSLLLE F LQSFLGSH TPGAPAVFGN RHDAVYGPAD TMVQYMELFN KIRKQQQVPV AGIR

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

Macromolecule #71: Mediator of RNA polymerase II transcription subunit 22

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 22; type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.243713 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILN DFPSVNEAID QRNQQLRTLQ EECDRKLITL RDEISIDLYE LEEEYYSSSS SLCEANDLPL CEAYGRLDLD T DSADGLSA PLLASPEPSA GPLQVAAPAH SHAGGPGPTE HA

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

Macromolecule #72: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16

• Macromolecule: Name: Isoform 2 of Mediator of RNA polymerase II transcription subunit 16; type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 93.136039 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR MIHILDTEHP WDLHSIPSEH HEAITCLEW DQSGSRLLSA DADGQIKCWS MADHLANSWE SSVGSLVEGD PIVALSWLHN GVKLALHVEK SGASSFGEKF S RVKFSPSL TLFGGKPMEG WIAVTVSGLV TVSLLKPSGQ VLTSTESLCR LRGRVALADI AFTGGGNIVV ATADGSSASP VQ FYKVCVS VVSEKCRIDT EILPSLFMRC TTDLNRKDKF PAITHLKFLA RDMSEQVLLC ASSQTSSIVE CWSLRKEGLP VNN IFQQIS PVVGDKQPTI LKWRILSATN DLDRVSAVAL PKLPISLTNT DLKVASDTQF YPGLGLALAF HDGSVHIVHR LSLQ TMAVF YSSAAPRPVD EPAMKRPRTA GPAVHLKAMQ LSWTSLALVG IDSHGKLSVL RLSPSMGHPL EVGLALRHLL FLLEY CMVT GYDWWDILLH VQPSMVQSLV EKLHEEYTRQ TAALQQVLST RILAMKASLC KLSPCTVTRV CDYHTKLFLI AISSTL KSL LRPHFLNTPD KSPGDRLTEI CTKITDVDID KVMINLKTEE FVLDMNTLQA LQQLLQWVGD FVLYLLASLP NQGSLLR PG HSFLRDGTSL GMLRELMVVI RIWGLLKPSC LPVYTATSDT QDSMSLLFRL LTKLWICCRD EGPASEPDEA LVDECCLL P SQLLIPSLDW LPASDGLVSR LQPKQPLRLQ FGRAPTLPGS AATLQLDGLA RAPGQPKIDH LRRLHLGACP TEECKACTR CGCVTMLKSP NRTTAVKQWE QRWIKNCLCG GLWWRVPLSY P

UniProtKB: Mediator of RNA polymerase II transcription subunit 16

Macromolecule #73: Mediator of RNA polymerase II transcription subunit 23

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 23; type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 156.652156 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

METQLQSIFE EVVKTEVIEE AFPGMFMDTP EDEKTKLISC LGAFRQFWGG LSQESHEQCI QWIVKFIHGQ HSPKRISFLY DCLAMAVET GLLPPRLVCE SLINSDTLEW ERTQLWALTF KLVRKIIGGV DYKGVRDLLK VILEKILTIP NTVSSAVVQQ L LAAREVIA YILERNACLL PAYFAVTEIR KLYPEGKLPH WLLGNLVSDF VDTFRPTARI NSICGRCSLL PVVNNSGAIC NS WKLDPAT LRFPLKGLLP YDKDLFEPQT ALLRYVLEQP YSRDMVCNML GLNKQHKQRC PVLEDQLVDL VVYAMERSET EEK FDDGGT SQLLWQHLSS QLIFFVLFQF ASFPHMVLSL HQKLAGRGLI KGRDHLMWVL LQFISGSIQK NALADFLPVM KLFD LLYPE KEYIPVPDIN KPQSTHAFAM TCIWIHLNRK AQNDNSKLQI PIPHSLRLHH EFLQQSLRNK SLQMNDYKIA LLCNA YSTN SECFTLPMGA LVETIYGNGI MRIPLPGTNC MASGSITPLP MNLLDSLTVH AKMSLIHSIA TRVIKLAHAK SSVALA PAL VETYSRLLVY MEIESLGIKG FISQLLPTVF KSHAWGILHT LLEMFSYRMH HIQPHYRVQL LSHLHTLAAV AQTNQNQ LH LCVESTALRL ITALGSSEVQ PQFTRFLSDP KTVLSAESEE LNRALILTLA RATHVTDFFT GSDSIQGTWC KDILQTIM S FTPHNWASHT LSCFPGPLQA FFKQNNVPQE SRFNLKKNVE EEYRKWKSMS NENDIITHFS MQGSPPLFLC LLWKMLLET DHINQIGYRV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLL LLKPNDFRNR VSDFVKENSP EHWLQNDWHT KHMNYHKKYP EKLYFEGLAE QVDPPVQIQS PYLPIYFGNV C LRFLPVFD IVIHRFLELL PVSKSLETLL DHLGGLYKFH DRPVTYLYNT LHYYEMHLRD RAFLKRKLVH AIIGSLKDNR PQ GWCLSDT YLKCAMNARE ENPWVPDDTY YCRLIGRLVD TMAGKSPGPF PNCDWRFNEF PNPAAHALHV TCVELMALAV SGK EVGNAL LNVVLKSQPL VPRENITAWM NAIGLIITAL PEPYWIVLHD RIVSVISSPS LTSETEWVGY PFRLFDFTAC HQSY SEMSC SYTLALAHAV WHHSSIGQLS LIPKFLTEVL LPIVKTEFQL LYVYHLVGPF LQRFQQERTR CMIEIGVAFY DMLLN VDQC STHLNYMDPI CDFLYHMKYM FTGDSVKEQV EKIICNLKPA LKLRLRFITH ISKMEPAAVP PQAMNSGSPA PQSNQV PVS LPVTQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 23

Macromolecule #74: Mediator of RNA polymerase II transcription subunit 24

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 24; type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 110.430164 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA MIGPSPNPLI LSYLKYAISS QMVSYSSVLT AISKFDDFS RDLCVQALLD IMDMFCDRLS CHGKAEECIG LCRALLSALH WLLRCTAASA ERLREGLEAG TPAAGEKQLA M CLQRLEKT LSSTKNRALL HIAKLEEASS WTAIEHSLLK LGEILANLSN PQLRSQAEQC GTLIRSIPTM LSVHAEQMHK TG FPTVHAV ILLEGTMNLT GETQSLVEQL TMVKRMQHIP TPLFVLEIWK ACFVGLIESP EGTEELKWTA FTFLKIPQVL VKL KKYSHG DKDFTEDVNC AFEFLLKLTP LLDKADQRCN CDCTNFLLQE CGKQGLLSEA SVNNLMAKRK ADREHAPQQK SGEN ANIQP NIQLILRAEP TVTNILKTMD ADHSKSPEGL LGVLGHMLSG KSLDLLLAAA AATGKLKSFA RKFINLNEFT TYGSE ESTK PASVRALLFD ISFLMLCHVA QTYGSEVILS ESRTGAEVPF FETWMQTCMP EEGKILNPDH PCFRPDSTKV ESLVAL LNN SSEMKLVQMK WHEACLSISA AILEILNAWE NGVLAFESIQ KITDNIKGKV CSLAVCAVAW LVAHVRMLGL DEREKSL QM IRQLAGPLFS ENTLQFYNER VVIMNSILER MCADVLQQTA TQIKFPSTGV DTMPYWNLLP PKRPIKEVLT DIFAKVLE K GWVDSRSIHI FDTLLHMGGV YWFCNNLIKE LLKETRKEHT LRAVELLYSI FCLDMQQVTL VLLGHILPGL LTDSSKWHS LMDPPGTALA KLAVWCALSS YSSHKGQAST RQKKRHREDI EDYISLFPLD DVQPSKLMRL LSSNEDDANI LSSPTDRSMS SSLSASQLH TVNMRDPLNR VLANLFLLIS SILGSRTAGP HTQFVQWFME ECVDCLEQGG RGSVLQFMPF TTVSELVKVS A MSSPKVVL AITDLSLPLG RQVAAKAIAA L

UniProtKB: Mediator of RNA polymerase II transcription subunit 24

Macromolecule #75: Mediator of RNA polymerase II transcription subunit 25

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 25; type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 78.230344 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTS SAYEFVTWLD GIKFMGGGGE SCSLIAEGLS TALQLFDDFK KMREQIGQTH RVCLLICNSP PYLLPAVEST T YSGCTTEN LVQQIGERGI HFSIVSPRKL PALRLLFEKA APPALLEPLQ PPTDVSQDPR HMVLVRGLVL PVGGGSAPGP LQ SKQPVPL PPAAPSGATL SAAPQQPLPP VPPQYQVPGN LSAAQVAAQN AVEAAKNQKA GLGPRFSPIT PLQQAAPGVG PPF SQAPAP QLPPGPPGAP KPPPASQPSL VSTVAPGSGL APTAQPGAPS MAGTVAPGGV SGPSPAQLGA PALGGQQSVS NKLL AWSGV LEWQEKPKPA SVDANTKLTR SLPCQVYVNH GENLKTEQWP QKLIMQLIPQ QLLTTLGPLF RNSRMVQFHF TNKDL ESLK GLYRIMGNGF AGCVHFPHTA PCEVRVLMLL YSSKKKIFMG LIPYDQSGFV NGIRQVITNH KQVQQQKLEQ QQRGMG GQQ APPGLGPILE DQARPSQNLL QLRPPQPQPQ GTVGASGATG QPQPQGTAQP PPGAPQGPPG AASGPPPPGP ILRPQNP GA NPQLRSLLLN PPPPQTGVPP PQASLHHLQP PGAPALLPPP HQGLGQPQLG PPLLHPPPAQ SWPAQLPPRA PLPGQMLL S GGPRGPVPQP GLQPSVMEDD ILMDLI

UniProtKB: Mediator of RNA polymerase II transcription subunit 25

Macromolecule #76: HISTONE H2A.Z

• Macromolecule: Name: HISTONE H2A.Z / type: protein_or_peptide / ID: 76 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.581796 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAGGKAGKDS GKAKTKAVSR SQRAGLQFPV GRIHRHLKSR TTSHGRVGAT AAVYSAAILE YLTAEVLELA GNASKDLKVK RITPRHLQL AIRGDEELDS LIKATIAGGG VIPHIHKSLI GKKGQQKTV

Macromolecule #77: Histone H2B

• Macromolecule: Name: Histone H2B / type: protein_or_peptide / ID: 77 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Xenopus laevis (African clawed frog)
• Molecular weight: Theoretical: 13.979291 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MPEPAKSAPA PKKGSKKAVT KTQKKDGKKR RKTRKESYAI YVYKVLKQVH PDTGISSKAM SIMNSFVNDV FERIAGEASR LAHYNKRST ITSREIQTAV RLLLPGELAK HAVSEGTKAV TKYTSAK

UniProtKB: Histone H2B

Macromolecule #78: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 78 / Number of copies: 19 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #79: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 79 / Number of copies: 1 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #80: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 80 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: OTHER
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.16 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 29473
• Initial angle assignment: Type: OTHER
• Final angle assignment: Type: MAXIMUM LIKELIHOOD