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Yorodumi- PDB-8gdg: Solution structure of the Neutrophil Serine Protease Inhibitor, EapH2 -
+Open data
-Basic information
Entry | Database: PDB / ID: 8gdg | |||||||||
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Title | Solution structure of the Neutrophil Serine Protease Inhibitor, EapH2 | |||||||||
Components | Cell surface like-protein Map-w | |||||||||
Keywords | IMMUNE SYSTEM/INHIBITOR / protease / protease inhibitor / enzyme kinetics / innate immunity / immune evasion / IMMUNE SYSTEM / IMMUNE SYSTEM-INHIBITOR complex | |||||||||
Function / homology | MAP domain / MAP domain / MAP repeat profile. / Cell surface like-protein Map-w Function and homology information | |||||||||
Biological species | Staphylococcus aureus (bacteria) | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Authors | Mishra, N.B. / Geisbrecht, B.V. / Prakash, O. | |||||||||
Funding support | United States, 2items
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Citation | Journal: J.Biol.Chem. / Year: 2023 Title: Simultaneous inhibition of two neutrophil serine proteases by the S. aureus innate immune evasion protein EapH2. Authors: Mishra, N. / Herdendorf, T.J. / Prakash, O. / Geisbrecht, B.V. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8gdg.cif.gz | 833.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8gdg.ent.gz | 709.9 KB | Display | PDB format |
PDBx/mmJSON format | 8gdg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8gdg_validation.pdf.gz | 407.6 KB | Display | wwPDB validaton report |
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Full document | 8gdg_full_validation.pdf.gz | 542.3 KB | Display | |
Data in XML | 8gdg_validation.xml.gz | 35.9 KB | Display | |
Data in CIF | 8gdg_validation.cif.gz | 57.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gd/8gdg ftp://data.pdbj.org/pub/pdb/validation_reports/gd/8gdg | HTTPS FTP |
-Related structure data
Related structure data | 8g24C 8g25C 8g26C 8gdhC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13123.912 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: map_1, NCTC10702_01500, SAGV69_01671 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A380EFL1 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 1 mM [U-100% 13C; U-100% 15N] EapH2, 95% H2O/5% D2O Details: 15N_13C_EapH2 was dissolved in 95% H2O/5% D2O / Label: 15N_13C_EapH2 / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 1 mM / Component: EapH2 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 50 mM / Label: 1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K / Temperature err: 0.5 |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 4 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 20 |