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- PDB-8ffe: Crystal structure of LRP6 E1E2 domains bound to YW210.09 Fab and ... -

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Basic information

Entry
Database: PDB / ID: 8ffe
TitleCrystal structure of LRP6 E1E2 domains bound to YW210.09 Fab and engineered XWnt8 peptide
Components
  • (YW210.09 Fab ...) x 2
  • Low-density lipoprotein receptor-related protein 6
KeywordsSIGNALING PROTEIN/Immune System / WNT / RECEPTOR / LRP5 / LRP6 / LDL RECEPTOR-LIKE PROTEIN / YWTD B-PROPELLER / SIGNALING PROTEIN / SIGNALING PROTEIN-Immune System complex
Function / homology
Function and homology information


Wnt signaling pathway involved in somitogenesis / Wnt-Frizzled-LRP5/6 complex / Negative regulation of TCF-dependent signaling by WNT ligand antagonists / Signaling by RNF43 mutants / neural crest formation / receptor-mediated endocytosis involved in cholesterol transport / kinase inhibitor activity / Wnt receptor activity / low-density lipoprotein particle receptor activity / toxin transmembrane transporter activity ...Wnt signaling pathway involved in somitogenesis / Wnt-Frizzled-LRP5/6 complex / Negative regulation of TCF-dependent signaling by WNT ligand antagonists / Signaling by RNF43 mutants / neural crest formation / receptor-mediated endocytosis involved in cholesterol transport / kinase inhibitor activity / Wnt receptor activity / low-density lipoprotein particle receptor activity / toxin transmembrane transporter activity / Wnt-protein binding / cellular response to cholesterol / midbrain dopaminergic neuron differentiation / negative regulation of protein serine/threonine kinase activity / dopaminergic neuron differentiation / frizzled binding / neural crest cell differentiation / Wnt signalosome / Disassembly of the destruction complex and recruitment of AXIN to the membrane / negative regulation of smooth muscle cell apoptotic process / canonical Wnt signaling pathway / coreceptor activity / positive regulation of cell cycle / Regulation of FZD by ubiquitination / TCF dependent signaling in response to WNT / protein localization to plasma membrane / cell-cell adhesion / response to peptide hormone / Wnt signaling pathway / positive regulation of DNA-binding transcription factor activity / positive regulation of cytosolic calcium ion concentration / chemical synaptic transmission / cytoplasmic vesicle / early endosome membrane / membrane raft / signaling receptor binding / neuronal cell body / synapse / positive regulation of DNA-templated transcription / cell surface / endoplasmic reticulum / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / extracellular region / identical protein binding / plasma membrane
Similarity search - Function
Low density lipoprotein receptor-related protein 5/6 / Low-density lipoprotein receptor repeat class B / LDL-receptor class B (LDLRB) repeat profile. / LDLR class B repeat / Low-density lipoprotein-receptor YWTD domain / Low-density lipoprotein receptor domain class A / Low-density lipoprotein (LDL) receptor class A, conserved site / LDL-receptor class A (LDLRA) domain signature. / LDL-receptor class A (LDLRA) domain profile. / Low-density lipoprotein receptor domain class A ...Low density lipoprotein receptor-related protein 5/6 / Low-density lipoprotein receptor repeat class B / LDL-receptor class B (LDLRB) repeat profile. / LDLR class B repeat / Low-density lipoprotein-receptor YWTD domain / Low-density lipoprotein receptor domain class A / Low-density lipoprotein (LDL) receptor class A, conserved site / LDL-receptor class A (LDLRA) domain signature. / LDL-receptor class A (LDLRA) domain profile. / Low-density lipoprotein receptor domain class A / Low-density lipoprotein (LDL) receptor class A repeat / LDL receptor-like superfamily / Six-bladed beta-propeller, TolB-like / Coagulation Factor Xa inhibitory site / Epidermal growth factor-like domain. / EGF-like domain signature 2. / EGF-like domain
Similarity search - Domain/homology
SUCCINIC ACID / Low-density lipoprotein receptor-related protein 6
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.72 Å
AuthorsJude, K.M. / Tsutsumi, N. / Waghray, D. / Garcia, K.C.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)R01DK115728 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2023
Title: Structure of the Wnt-Frizzled-LRP6 initiation complex reveals the basis for coreceptor discrimination.
Authors: Naotaka Tsutsumi / Sunhee Hwang / Deepa Waghray / Simon Hansen / Kevin M Jude / Nan Wang / Yi Miao / Caleb R Glassman / Nathanael A Caveney / Claudia Y Janda / Rami N Hannoush / K Christopher Garcia /
Abstract: Wnt morphogens are critical for embryonic development and tissue regeneration. Canonical Wnts form ternary receptor complexes composed of tissue-specific Frizzled (Fzd) receptors together with the ...Wnt morphogens are critical for embryonic development and tissue regeneration. Canonical Wnts form ternary receptor complexes composed of tissue-specific Frizzled (Fzd) receptors together with the shared LRP5/6 coreceptors to initiate β-catenin signaling. The cryo-EM structure of a ternary initiation complex of an affinity-matured XWnt8-Frizzled8-LRP6 complex elucidates the basis of coreceptor discrimination by canonical Wnts by means of their N termini and linker domains that engage the LRP6 E1E2 domain funnels. Chimeric Wnts bearing modular linker "grafts" were able to transfer LRP6 domain specificity between different Wnts and enable non-canonical Wnt5a to signal through the canonical pathway. Synthetic peptides comprising the linker domain serve as Wnt-specific antagonists. The structure of the ternary complex provides a topological blueprint for the orientation and proximity of Frizzled and LRP6 within the Wnt cell surface signalosome.
History
DepositionDec 8, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 8, 2023Provider: repository / Type: Initial release
Revision 1.1Mar 22, 2023Group: Data collection / Database references / Category: citation / citation_author / diffrn_detector
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name / _diffrn_detector.pdbx_collection_date

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Low-density lipoprotein receptor-related protein 6
H: YW210.09 Fab heavy chain with engineered XWnt8 NC peptide and linker
L: YW210.09 Fab light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,40017
Polymers122,3863
Non-polymers3,01414
Water10,665592
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11200 Å2
ΔGint-11 kcal/mol
Surface area44440 Å2
MethodPISA
Unit cell
Length a, b, c (Å)125.212, 91.385, 104.055
Angle α, β, γ (deg.)90.000, 103.670, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z

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Components

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Protein , 1 types, 1 molecules A

#1: Protein Low-density lipoprotein receptor-related protein 6 / LRP-6


Mass: 69302.148 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: LRP6 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: O75581

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Antibody , 2 types, 2 molecules HL

#2: Antibody YW210.09 Fab heavy chain with engineered XWnt8 NC peptide and linker


Mass: 28657.805 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: engineered Wnt8 peptide (position 1-18) is originally from Xenopus laevis
Source: (gene. exp.) Homo sapiens (human) / Production host: Trichoplusia ni (cabbage looper)
#3: Antibody YW210.09 Fab light chain


Mass: 24426.025 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Trichoplusia ni (cabbage looper)

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Sugars , 3 types, 3 molecules

#4: Polysaccharide alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1- ...alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 716.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
LFucpa1-3[DGlcpNAcb1-4][LFucpa1-6]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2-1-2/a3-b1_a4-c1_a6-d1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE
#5: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 878.823 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4[LFucpa1-3][LFucpa1-6]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5][a1122h-1b_1-5]/1-2-1-3-2/a3-b1_a4-c1_a6-e1_c4-d1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(3+1)][a-L-Fucp]{}[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE
#6: Polysaccharide alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy- ...alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DGlcpNAcb1-4]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1a_1-5]/1-2-1/a3-b1_a4-c1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(3+1)][a-D-Manp]{}[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE

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Non-polymers , 4 types, 603 molecules

#7: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C3H8O3
#8: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Na
#9: Chemical ChemComp-SIN / SUCCINIC ACID / Succinic acid


Mass: 118.088 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H6O4
#10: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 592 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.36 Å3/Da / Density % sol: 47.95 % / Description: rectangluar prism
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6
Details: 100 mM SPG (succinate/phosphate/glycine buffer), 25% PEG 1500

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-1 / Wavelength: 0.97946 Å
DetectorType: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Nov 14, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97946 Å / Relative weight: 1
ReflectionResolution: 1.72→44.37 Å / Num. obs: 109415 / % possible obs: 90.56 % / Redundancy: 7.1 % / Biso Wilson estimate: 30.74 Å2 / CC1/2: 0.997 / CC star: 0.999 / Rpim(I) all: 0.03889 / Rrim(I) all: 0.1042 / Rsym value: 0.09658 / Net I/σ(I): 10.49
Reflection shellResolution: 1.72→1.781 Å / Redundancy: 6.7 % / Mean I/σ(I) obs: 0.64 / Num. unique obs: 6719 / CC1/2: 0.426 / CC star: 0.773 / Rpim(I) all: 0.8556 / Rrim(I) all: 2.243 / Rsym value: 2.068 / % possible all: 55.47

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
XDSdata reduction
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.72→44.37 Å / SU ML: 0.2478 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 27.5285
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2308 5465 5 %
Rwork0.185 103836 -
obs0.1873 109301 90.56 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 38.7 Å2
Refinement stepCycle: LAST / Resolution: 1.72→44.37 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8272 0 199 592 9063
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00928713
X-RAY DIFFRACTIONf_angle_d0.973711859
X-RAY DIFFRACTIONf_chiral_restr0.06351354
X-RAY DIFFRACTIONf_plane_restr0.00921506
X-RAY DIFFRACTIONf_dihedral_angle_d13.77913172
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.72-1.740.50221030.46621892X-RAY DIFFRACTION50.82
1.74-1.760.44980.41072134X-RAY DIFFRACTION54.83
1.76-1.780.44081270.38832269X-RAY DIFFRACTION60.83
1.78-1.80.40961290.35052612X-RAY DIFFRACTION67.38
1.8-1.830.33531520.3032826X-RAY DIFFRACTION74.52
1.83-1.850.37611690.27833281X-RAY DIFFRACTION86.51
1.85-1.880.30281920.25073567X-RAY DIFFRACTION93.93
1.88-1.910.30041920.25233652X-RAY DIFFRACTION95.6
1.91-1.940.3231930.23933687X-RAY DIFFRACTION96.95
1.94-1.970.26821940.21023677X-RAY DIFFRACTION97.33
1.97-20.26661960.19893721X-RAY DIFFRACTION97.29
2-2.040.25731970.20043779X-RAY DIFFRACTION97.45
2.04-2.080.22921930.19773697X-RAY DIFFRACTION97.47
2.08-2.120.26421910.19823704X-RAY DIFFRACTION97.42
2.12-2.170.24851980.20173729X-RAY DIFFRACTION97.3
2.17-2.220.24451940.19253712X-RAY DIFFRACTION97.48
2.22-2.270.26851940.21833691X-RAY DIFFRACTION96.74
2.27-2.330.23881790.20423416X-RAY DIFFRACTION90.21
2.33-2.40.24351960.20643665X-RAY DIFFRACTION95.66
2.4-2.480.31761980.20133757X-RAY DIFFRACTION98.33
2.48-2.570.23991980.19493752X-RAY DIFFRACTION98.21
2.57-2.670.2442020.19533778X-RAY DIFFRACTION98.47
2.67-2.790.25592000.19823732X-RAY DIFFRACTION98.2
2.79-2.940.23982010.2093776X-RAY DIFFRACTION98.39
2.94-3.130.26561940.20313746X-RAY DIFFRACTION97.6
3.13-3.370.23911880.19093537X-RAY DIFFRACTION91.86
3.37-3.70.21691940.17053698X-RAY DIFFRACTION96.74
3.71-4.240.18742000.15163793X-RAY DIFFRACTION98.98
4.24-5.340.16842060.13233829X-RAY DIFFRACTION98.66
5.34-44.370.18481970.15943727X-RAY DIFFRACTION94.67
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.353775301740.570870098137-0.2227702022073.341087470710.1450524727122.635143673820.0858838781546-0.487440632132-0.05077976762550.525478702034-0.109675069232-0.6915778690970.01172780949620.431646082375-0.08033562093940.329462253126-0.058972893183-0.1136194609630.462806260528-0.01008236532480.34170148366511.6163247333-28.115042899495.2521976211
21.156989393780.372669369602-0.2743703290912.45384665881-0.1439254226231.090503697880.169934425558-0.3542724492930.2786876915150.613659109145-0.03617107513130.0263311328808-0.2016960075320.0974473366431-0.1306271039030.411353003733-0.04476019916130.04763000600050.377900776269-0.04940015849170.221530392974-4.93134615268-23.421186714798.8964403265
31.05832881071-0.0914297848294-0.06200295443220.6701911112270.1816186246160.631252144489-0.02572813977940.0014815982956-0.0615388352466-0.02080581421730.00847478548990.02102536418750.03262724836890.04822458261790.02193134264490.239173100818-0.02376700754790.01134675470620.2052937551020.02442796716170.196827876203-17.7511678131-55.735173152869.3935738914
45.358258102724.97193928527-4.365467720474.80461976094-3.145224393627.84713772396-0.218429266651.059995364470.683876733457-0.7230529922920.4245914366690.00584222830147-0.4609087823370.208981357294-0.01480552579680.388260636822-0.04752271685950.071874200910.3779995982910.08011917299720.432471733308-14.6748730898-41.678975346455.3964256714
55.711817330266.84047910198-4.562511928158.49328607835-5.368891777283.996054728910.264273248246-0.26489982490.4206353117420.3219566334090.0715479825943-0.913905236202-0.4803683549531.01064104242-0.1118469602560.81024978475-0.239938823637-0.0524042298980.7011748125690.1250343875730.984561236815-7.83695017876-37.682148913257.6839079592
66.791928895081.8024002475-1.84469515866.11167952546-1.776340520215.69004234367-0.6133845137111.13216467731-0.619415690004-0.5545890782530.4672673521570.8820261062910.685717516038-0.954745218897-0.06518332864590.4072323134590.0195599466621-0.02840520625280.438087348886-0.09277402386550.454977103414-5.93770973204-10.398327173161.7236264015
71.490809707810.757760243142-0.8204808680381.81573897465-0.7277384631252.169675637210.04964884165140.00697861274510.05625394181220.0844699110743-0.007292505880980.233543813362-0.0363745111472-0.0754885617743-0.009477398543210.1952136686370.0107395832520.01769581314070.173543676053-0.01324409401890.2309341229945.9893023127-5.0432830050367.5790737909
81.62750210440.2332156470890.05552861429712.02273920060.1891718510583.76464340507-0.05379020303040.324328081408-0.00223352114316-0.233766623549-0.03030465501990.448218934036-0.227200251291-0.463536620649-0.01146236432640.2211008998060.0182302505837-0.04546956663230.312628313256-0.05423422757470.3371675949875.64522655307-3.8726875199233.5886944729
91.381854441110.632427378680.7156385271331.394734999451.073143002125.70420222431-0.0297170746789-0.0160841782074-0.1162449244620.0115303686886-0.07449387120780.001826688765750.3019401342330.1479546157230.0405866666010.2260550532570.03883222873970.01838008186920.193269593273-0.0155752547860.35066742550118.3182186672-26.158499548460.6835569546
101.30599524730.1236664314760.1248519777950.76657225148-0.1123416533563.54401990250.05681740535090.00277281071376-0.262852706308-0.0537919533176-0.04552744665160.1109139539030.119571846693-0.124149107589-0.01595646588180.245722404618-0.00100827202249-7.22124761467E-50.200284063137-0.01844256173820.3700660128429.97439162767-24.773285501762.2780204268
111.127734376490.633132181646-0.4073208616413.01202196644-1.623306428294.417080821250.02991409528830.2884313541530.0604979318188-0.3661895534260.0696791854410.3441737166740.00982919677711-0.105458373145-0.08306827868520.172240167181-0.00962398847859-0.03276004066130.330386095972-0.05150608584540.25835026188413.3636698766-14.447193058728.8077711552
122.214366863772.57694746321-1.532417521366.13560713344-2.578817936134.83010000296-0.02522764195010.217310124789-0.148808589917-0.163007082852-0.0453123710124-0.0981787976551-0.1446488594140.2387648757990.1534451308830.218671610553-0.00922633033754-0.03113043911010.287121801655-0.06787087666930.24130545060619.6682880677-11.176894887828.963530204
133.154917788592.56239434936-2.555199320887.67252433305-2.852375863634.2129371432-0.01951784139820.166986118075-0.07500958724050.4071339317270.0899997549550.323653190549-0.02021747825920.04909985648290.03495561732510.2235269557870.0205234748148-0.008967195515640.271871170182-0.05460413212570.27112971681517.6944836301-12.75173449734.6973833494
143.735088221162.42393102387-2.06202286818.29490017283-1.71131337994.2079187095-0.2017476396150.677586539242-0.231330980446-1.124372311380.00639237154695-0.1942384987180.161624350028-0.2054812195980.04154836856680.362790987920.0005758949818110.004293545569410.466641334933-0.07449042519080.2516716643321.924911855-8.2673770067920.2467054376
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11(chain A and resid 20:93)AA20 - 931 - 74
22(chain A and resid 94:324)AA94 - 32475 - 305
33(chain A and resid 325:632)AA325 - 632306 - 613
44(chain H and resid 226:234)HR226 - 2341 - 9
55(chain H and resid 235:240)HR235 - 24010 - 15
66(chain H and resid 1000:1006)HR1000 - 100616 - 22
77(chain H and resid 1007:1114)HR1007 - 111423 - 143
88(chain H and resid 1115:1214)HR1115 - 1214144 - 239
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1111(chain L and resid 106:124)LT106 - 124109 - 127
1212(chain L and resid 125:152)LT125 - 152128 - 155
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