+Open data
-Basic information
Entry | Database: PDB / ID: 8f6l | ||||||
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Title | anti-BTLA monoclonal antibody h25F7 in complex with BTLA | ||||||
Components |
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Keywords | IMMUNE SYSTEM / monoclonal antibody / complex | ||||||
Function / homology | Function and homology information immune response-regulating cell surface receptor signaling pathway / Costimulation by the CD28 family / signaling receptor activity / adaptive immune response / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.85 Å | ||||||
Authors | Hendle, J. / Atwell, S. / Lieu, R. / Hickey, M. / Weichert, K. | ||||||
Funding support | 1items
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Citation | Journal: Structure / Year: 2023 Title: Epitope topography of agonist antibodies to the checkpoint inhibitory receptor BTLA. Authors: Cheung, T.C. / Atwell, S. / Bafetti, L. / Cuenca, P.D. / Froning, K. / Hendle, J. / Hickey, M. / Ho, C. / Huang, J. / Lieu, R. / Lim, S. / Lippner, D. / Obungu, V. / Ward-Kavanagh, L. / ...Authors: Cheung, T.C. / Atwell, S. / Bafetti, L. / Cuenca, P.D. / Froning, K. / Hendle, J. / Hickey, M. / Ho, C. / Huang, J. / Lieu, R. / Lim, S. / Lippner, D. / Obungu, V. / Ward-Kavanagh, L. / Weichert, K. / Ware, C.F. / Vendel, A.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8f6l.cif.gz | 129.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8f6l.ent.gz | 96.3 KB | Display | PDB format |
PDBx/mmJSON format | 8f6l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8f6l_validation.pdf.gz | 435.6 KB | Display | wwPDB validaton report |
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Full document | 8f6l_full_validation.pdf.gz | 435.7 KB | Display | |
Data in XML | 8f6l_validation.xml.gz | 24.5 KB | Display | |
Data in CIF | 8f6l_validation.cif.gz | 36.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f6/8f6l ftp://data.pdbj.org/pub/pdb/validation_reports/f6/8f6l | HTTPS FTP |
-Related structure data
Related structure data | 8f60C 8f6oC 2aw2S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 25036.982 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster) |
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#2: Antibody | Mass: 23509.982 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster) |
#3: Protein | Mass: 15147.877 Da / Num. of mol.: 1 / Fragment: Extracellular domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BTLA / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: Q7Z6A9 |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.2 % |
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Crystal grow | Temperature: 281 K / Method: vapor diffusion, sitting drop Details: 18% PEG 5000 MME + 200mM Magnesium Formate dihydrate + 100mM Sodium Acetate pH 4.6 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.97931 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 9, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97931 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→64 Å / Num. obs: 54860 / % possible obs: 99.8 % / Redundancy: 7.2 % / Rmerge(I) obs: 0.083 / Net I/σ(I): 15.7 |
Reflection shell | Resolution: 1.85→1.95 Å / Redundancy: 7.4 % / Rmerge(I) obs: 0.672 / Mean I/σ(I) obs: 2.8 / Num. unique obs: 7911 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2AW2 Resolution: 1.85→30 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.934 / SU B: 3.632 / SU ML: 0.107 / Cross valid method: THROUGHOUT / ESU R: 0.136 / ESU R Free: 0.133 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.162 Å2
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Refinement step | Cycle: LAST / Resolution: 1.85→30 Å
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