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Open data
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Basic information
Entry | Database: PDB / ID: 8f6o | ||||||
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Title | anti-BTLA monoclonal antibody h22B3 in complex with BTLA | ||||||
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![]() | IMMUNE SYSTEM / monoclonal antibody / complex | ||||||
Function / homology | ![]() immune response-regulating cell surface receptor signaling pathway / Costimulation by the CD28 family / signaling receptor activity / adaptive immune response / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hendle, J. / Atwell, S. / Lieu, R. / Hickey, M. / Weichert, K. | ||||||
Funding support | 1items
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![]() | ![]() Title: Epitope topography of agonist antibodies to the checkpoint inhibitory receptor BTLA. Authors: Cheung, T.C. / Atwell, S. / Bafetti, L. / Cuenca, P.D. / Froning, K. / Hendle, J. / Hickey, M. / Ho, C. / Huang, J. / Lieu, R. / Lim, S. / Lippner, D. / Obungu, V. / Ward-Kavanagh, L. / ...Authors: Cheung, T.C. / Atwell, S. / Bafetti, L. / Cuenca, P.D. / Froning, K. / Hendle, J. / Hickey, M. / Ho, C. / Huang, J. / Lieu, R. / Lim, S. / Lippner, D. / Obungu, V. / Ward-Kavanagh, L. / Weichert, K. / Ware, C.F. / Vendel, A.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 122.1 KB | Display | ![]() |
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PDB format | ![]() | 91.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.6 KB | Display | ![]() |
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Full document | ![]() | 452.7 KB | Display | |
Data in XML | ![]() | 21.6 KB | Display | |
Data in CIF | ![]() | 30.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8f60C ![]() 8f6lC ![]() 2aw2S C: citing same article ( S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 25083.943 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Antibody | Mass: 23423.910 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Protein | Mass: 15147.877 Da / Num. of mol.: 1 / Fragment: Extracellular domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.78 Å3/Da / Density % sol: 55.82 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop Details: 23% PEG 10K + 200mM Magnesium Formate + 100mM Sodium Acetate pH 5.4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 21, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97931 Å / Relative weight: 1 |
Reflection | Resolution: 2.31→54 Å / Num. obs: 31602 / % possible obs: 99.9 % / Redundancy: 6 % / Rmerge(I) obs: 0.093 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 2.31→2.43 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.505 / Mean I/σ(I) obs: 3.4 / Num. unique obs: 4567 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2AW2 Resolution: 2.31→54 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 2.31→54 Å
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Refine LS restraints |
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