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- PDB-8f3z: Crystal structure of Penicillin Binding Protein 5 (PBP5) S422A va... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8f3z | ||||||
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Title | Crystal structure of Penicillin Binding Protein 5 (PBP5) S422A variant apo form from Enterococcus faecium | ||||||
![]() | Penicillin binding protein 5 | ||||||
![]() | ANTIBIOTIC / Penicillin binding / antibiotic resistance / antibiotics | ||||||
Function / homology | ![]() peptidoglycan L,D-transpeptidase activity / penicillin binding / cell wall organization / response to antibiotic / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schoenle, M.V. / D'Andrea, E.D. / Choy, M.S. / Peti, W. / Page, R. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The Molecular Basis for Resistance of E. faecium PBP5 to beta-lactam Antibiotics Authors: Hunashal, Y. / Kumar, G.S. / Choy, M.S. / Da Silva Santiago, A. / D'Andrea, E.D. / Schoenle, M.V. / Arthur, M. / Rice, L.B. / Page, R. / Peti, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 145 KB | Display | ![]() |
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PDB format | ![]() | 109.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 444.1 KB | Display | ![]() |
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Full document | ![]() | 448.3 KB | Display | |
Data in XML | ![]() | 25.5 KB | Display | |
Data in CIF | ![]() | 36.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8f3fC ![]() 8f3gC ![]() 8f3hC ![]() 8f3iC ![]() 8f3jC ![]() 8f3lC ![]() 8f3mC ![]() 8f3nC ![]() 8f3oC ![]() 8f3pC ![]() 8f3qC ![]() 8f3rC ![]() 8f3sC ![]() 8f3tC ![]() 8f3uC ![]() 8f67C ![]() 6mkaS C: citing same article ( S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 70100.023 Da / Num. of mol.: 1 / Mutation: S422A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.84 Å3/Da / Density % sol: 78.95 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 0.095 M Tris pH 8.0, 2.85 M ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 26, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97946 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→39.5 Å / Num. obs: 41759 / % possible obs: 99.8 % / Redundancy: 19.8 % / CC1/2: 0.995 / Rmerge(I) obs: 0.351 / Rpim(I) all: 0.08 / Rrim(I) all: 0.36 / Χ2: 0.99 / Net I/σ(I): 14.3 / Num. measured all: 827832 |
Reflection shell | Resolution: 2.8→2.91 Å / % possible obs: 99.2 % / Redundancy: 19.2 % / Rmerge(I) obs: 3.355 / Num. measured all: 87971 / Num. unique obs: 4582 / CC1/2: 0.616 / Rpim(I) all: 0.779 / Rrim(I) all: 3.447 / Χ2: 0.91 / Net I/σ(I) obs: 3.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6MKA Resolution: 2.8→39.03 Å / SU ML: 0.34 / Cross valid method: THROUGHOUT / σ(F): 1.91 / Phase error: 21.26 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→39.03 Å
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Refine LS restraints |
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LS refinement shell |
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