- PDB-8eho: PRRSV-1 PLP2 domain bound to ubiquitin -
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Basic information
Entry
Database: PDB / ID: 8eho
Title
PRRSV-1 PLP2 domain bound to ubiquitin
Components
Papain-like protease 2
Ubiquitin
Keywords
VIRAL PROTEIN / PLP2 / DUB / deubiquitinating
Function / homology
Function and homology information
: / hypothalamus gonadotrophin-releasing hormone neuron development / female meiosis I / positive regulation of protein monoubiquitination / mitochondrion transport along microtubule / fat pad development / female gonad development / seminiferous tubule development / male meiosis I / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator ...: / hypothalamus gonadotrophin-releasing hormone neuron development / female meiosis I / positive regulation of protein monoubiquitination / mitochondrion transport along microtubule / fat pad development / female gonad development / seminiferous tubule development / male meiosis I / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / host cell membrane / energy homeostasis / regulation of neuron apoptotic process / regulation of proteasomal protein catabolic process / Maturation of protein E / Maturation of protein E / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Membrane binding and targetting of GAG proteins / Endosomal Sorting Complex Required For Transport (ESCRT) / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Negative regulation of FLT3 / Constitutive Signaling by NOTCH1 HD Domain Mutants / Regulation of FZD by ubiquitination / TICAM1,TRAF6-dependent induction of TAK1 complex / NOTCH2 Activation and Transmission of Signal to the Nucleus / TICAM1-dependent activation of IRF3/IRF7 / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / APC/C:Cdc20 mediated degradation of Cyclin B / p75NTR recruits signalling complexes / Downregulation of ERBB4 signaling / APC-Cdc20 mediated degradation of Nek2A / PINK1-PRKN Mediated Mitophagy / TRAF6-mediated induction of TAK1 complex within TLR4 complex / TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling / Pexophagy / Regulation of innate immune responses to cytosolic DNA / InlA-mediated entry of Listeria monocytogenes into host cells / VLDLR internalisation and degradation / Downregulation of ERBB2:ERBB3 signaling / NF-kB is activated and signals survival / NRIF signals cell death from the nucleus / Regulation of PTEN localization / Activated NOTCH1 Transmits Signal to the Nucleus / Regulation of BACH1 activity / Translesion synthesis by REV1 / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Translesion synthesis by POLK / MAP3K8 (TPL2)-dependent MAPK1/3 activation / TICAM1, RIP1-mediated IKK complex recruitment / Downregulation of TGF-beta receptor signaling / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / Josephin domain DUBs / neuron projection morphogenesis / Regulation of activated PAK-2p34 by proteasome mediated degradation / InlB-mediated entry of Listeria monocytogenes into host cell / IKK complex recruitment mediated by RIP1 / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / regulation of mitochondrial membrane potential / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Autodegradation of Cdh1 by Cdh1:APC/C / TNFR1-induced NF-kappa-B signaling pathway / APC/C:Cdc20 mediated degradation of Securin / positive regulation of protein ubiquitination / Asymmetric localization of PCP proteins / TCF dependent signaling in response to WNT / SCF-beta-TrCP mediated degradation of Emi1 / NIK-->noncanonical NF-kB signaling / Ubiquitin-dependent degradation of Cyclin D / Regulation of NF-kappa B signaling / AUF1 (hnRNP D0) binds and destabilizes mRNA / TNFR2 non-canonical NF-kB pathway / activated TAK1 mediates p38 MAPK activation / Assembly of the pre-replicative complex / Vpu mediated degradation of CD4 / NOTCH3 Activation and Transmission of Signal to the Nucleus / Negative regulators of DDX58/IFIH1 signaling / Deactivation of the beta-catenin transactivating complex / Degradation of DVL / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Regulation of signaling by CBL / Dectin-1 mediated noncanonical NF-kB signaling / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / Fanconi Anemia Pathway / Hh mutants are degraded by ERAD / Recognition of DNA damage by PCNA-containing replication complex / Negative regulation of FGFR3 signaling / Peroxisomal protein import / Degradation of AXIN Similarity search - Function
Mass: 22067.125 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Porcine reproductive and respiratory syndrome virus Production host: Escherichia coli (E. coli) / References: UniProt: W0NX70
#2: Protein
Ubiquitin
Mass: 8519.778 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: UBB / Production host: Escherichia coli (E. coli) / References: UniProt: P0CG47
Resolution: 2.85→43.94 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.879 / SU B: 15.682 / SU ML: 0.273 / Cross valid method: THROUGHOUT / ESU R: 2.75 / ESU R Free: 0.359 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.24658
1156
4.7 %
RANDOM
Rwork
0.21247
-
-
-
obs
0.21408
23538
96.45 %
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Solvent computation
Ion probe radii: 0.9 Å / Shrinkage radii: 0.9 Å / VDW probe radii: 1 Å / Solvent model: MASK
Displacement parameters
Biso mean: 44.2 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-2.57 Å2
-0 Å2
-0 Å2
2-
-
2.14 Å2
-0 Å2
3-
-
-
0.42 Å2
Refinement step
Cycle: LAST / Resolution: 2.85→43.94 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
5577
0
61
172
5810
Refine LS restraints
Refine-ID
Type
Dev ideal
Dev ideal target
Number
X-RAY DIFFRACTION
r_bond_refined_d
0.006
0.017
5766
X-RAY DIFFRACTION
r_bond_other_d
0.001
0.019
5512
X-RAY DIFFRACTION
r_angle_refined_deg
1.18
1.878
7842
X-RAY DIFFRACTION
r_angle_other_deg
0.954
2.64
12739
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
5.937
5
715
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
30.674
22.366
279
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
14.202
15
950
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
16.624
15
39
X-RAY DIFFRACTION
r_chiral_restr
0.061
0.2
883
X-RAY DIFFRACTION
r_gen_planes_refined
0.003
0.02
6407
X-RAY DIFFRACTION
r_gen_planes_other
0.001
0.02
1219
X-RAY DIFFRACTION
r_mcbond_it
0.972
4.485
2878
X-RAY DIFFRACTION
r_mcbond_other
0.973
4.485
2876
X-RAY DIFFRACTION
r_mcangle_it
1.756
6.721
3587
X-RAY DIFFRACTION
r_mcangle_other
1.756
6.721
3587
X-RAY DIFFRACTION
r_scbond_it
0.772
4.565
2888
X-RAY DIFFRACTION
r_scbond_other
0.772
4.566
2886
X-RAY DIFFRACTION
r_scangle_other
1.417
6.802
4253
X-RAY DIFFRACTION
r_long_range_B_refined
4.217
83.903
22832
X-RAY DIFFRACTION
r_long_range_B_other
4.205
83.979
22786
LS refinement shell
Resolution: 2.85→2.924 Å / Total num. of bins used: 20
Rfactor
Num. reflection
% reflection
Rfree
0.4
92
-
Rwork
0.371
1731
-
obs
-
-
99.08 %
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