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- PDB-8dx4: Clostridioides difficile R20291 minor pilin - PilW fused with Mal... -

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Basic information

Entry
Database: PDB / ID: 8dx4
TitleClostridioides difficile R20291 minor pilin - PilW fused with Maltose Binding Protein
ComponentsMaltodextrin-binding protein,Putative pilin protein
KeywordsDNA BINDING PROTEIN / Membrane Bound Protein / Pilin
Function / homology
Function and homology information


protein secretion by the type II secretion system / type II protein secretion system complex / cell envelope / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / periplasmic space / metal ion binding / plasma membrane
Similarity search - Function
PilW, C-terminal / Bacterial general secretion pathway protein G-type pilin / : / Prokaryotic N-terminal methylation site. / Prokaryotic N-terminal methylation motif / Prokaryotic N-terminal methylation site / Pilin-like / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. ...PilW, C-terminal / Bacterial general secretion pathway protein G-type pilin / : / Prokaryotic N-terminal methylation site. / Prokaryotic N-terminal methylation motif / Prokaryotic N-terminal methylation site / Pilin-like / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain
Similarity search - Domain/homology
DI(HYDROXYETHYL)ETHER / TRIETHYLENE GLYCOL / Pilin protein / Maltodextrin-binding protein
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Clostridioides difficile R20291 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.487 Å
AuthorsRonish, L.A. / Piepenbrink, K.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)K22 AI123467 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P20-GM113126 United States
CitationJournal: J.Biol.Chem. / Year: 2022
Title: Recognition of extracellular DNA by type IV pili promotes biofilm formation by Clostridioides difficile.
Authors: Ronish, L.A. / Sidner, B. / Yu, Y. / Piepenbrink, K.H.
History
DepositionAug 2, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 21, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 26, 2022Group: Database references / Category: citation / Item: _citation.journal_volume
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Maltodextrin-binding protein,Putative pilin protein
B: Maltodextrin-binding protein,Putative pilin protein
C: Maltodextrin-binding protein,Putative pilin protein
D: Maltodextrin-binding protein,Putative pilin protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)225,73725
Polymers224,1264
Non-polymers1,61221
Water11,259625
1
A: Maltodextrin-binding protein,Putative pilin protein
hetero molecules


  • defined by author
  • Evidence: gel filtration, Tandem liquid chromatography. First NiNTA column is followed by a size exclusion column. Fractions were then quantified for protein present by UV-vis wavelength of 280nm. ...Evidence: gel filtration, Tandem liquid chromatography. First NiNTA column is followed by a size exclusion column. Fractions were then quantified for protein present by UV-vis wavelength of 280nm. Followed by reducing SDS-PAGE to confirm the expected size.
  • 56.3 kDa, 1 polymers
  • Search similar-shape structures of this assembly by Omokage search (details)
Theoretical massNumber of molelcules
Total (without water)56,2805
Polymers56,0311
Non-polymers2484
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Maltodextrin-binding protein,Putative pilin protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,2805
Polymers56,0311
Non-polymers2484
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Maltodextrin-binding protein,Putative pilin protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,4565
Polymers56,0311
Non-polymers4254
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Maltodextrin-binding protein,Putative pilin protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)56,72210
Polymers56,0311
Non-polymers6919
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)65.326, 81.796, 102.964
Angle α, β, γ (deg.)92.440, 90.950, 113.370
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 2 through 21 or resid 23...
21(chain C and (resid 2 or (resid 3 and (name...
31(chain D and (resid 2 or (resid 3 and (name...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and (resid 2 through 21 or resid 23...A2 - 21
121(chain A and (resid 2 through 21 or resid 23...A23 - 24
131(chain A and (resid 2 through 21 or resid 23...A27
141(chain A and (resid 2 through 21 or resid 23...A29 - 40
151(chain A and (resid 2 through 21 or resid 23...A42 - 43
161(chain A and (resid 2 through 21 or resid 23...A45
171(chain A and (resid 2 through 21 or resid 23...A47 - 48
181(chain A and (resid 2 through 21 or resid 23...A1 - 1152
191(chain A and (resid 2 through 21 or resid 23...A56 - 137
1101(chain A and (resid 2 through 21 or resid 23...A0
1111(chain A and (resid 2 through 21 or resid 23...A225 - 2357
1121(chain A and (resid 2 through 21 or resid 23...A275 - 294
1131(chain A and (resid 2 through 21 or resid 23...A295
1141(chain A and (resid 2 through 21 or resid 23...A1 - 1152
1151(chain A and (resid 2 through 21 or resid 23...A1 - 1152
1161(chain A and (resid 2 through 21 or resid 23...A1 - 1152
1171(chain A and (resid 2 through 21 or resid 23...A1 - 1152
211(chain C and (resid 2 or (resid 3 and (name...C2
221(chain C and (resid 2 or (resid 3 and (name...C3
231(chain C and (resid 2 or (resid 3 and (name...C1 - 1152
241(chain C and (resid 2 or (resid 3 and (name...C1 - 1152
251(chain C and (resid 2 or (resid 3 and (name...C1 - 1152
261(chain C and (resid 2 or (resid 3 and (name...C1 - 1152
311(chain D and (resid 2 or (resid 3 and (name...D2
321(chain D and (resid 2 or (resid 3 and (name...D3
331(chain D and (resid 2 or (resid 3 and (name...D0 - 1152
341(chain D and (resid 2 or (resid 3 and (name...D0 - 1152
351(chain D and (resid 2 or (resid 3 and (name...D0 - 1152
361(chain D and (resid 2 or (resid 3 and (name...D0 - 1152

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
Maltodextrin-binding protein,Putative pilin protein


Mass: 56031.410 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli), (gene. exp.) Clostridioides difficile R20291 (bacteria)
Strain: FS14, CD196 / Gene: malE, JW3994, CD196_2148 / Plasmid: pETMAL / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: A0A4P1LXE0, UniProt: A0A0H3N4W9

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Non-polymers , 5 types, 646 molecules

#2: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 18 / Source method: obtained synthetically / Formula: C2H6O2
#3: Chemical ChemComp-EPE / 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES


Mass: 238.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H18N2O4S / Comment: pH buffer*YM
#4: Chemical ChemComp-PGE / TRIETHYLENE GLYCOL


Mass: 150.173 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H14O4
#5: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 625 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.25 Å3/Da / Density % sol: 45.33 %
Crystal growTemperature: 278 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: Hepes, Cesium Chloride, PEG 3350, n-Octyl-beta-D-glucoside, Sodium Chloride, ethanol

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Data collection

DiffractionMean temperature: 80 K / Ambient temp details: liquid nitrogen stream / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 30, 2021 / Details: dimorph mirrors
RadiationMonochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.48→102.8 Å / Num. obs: 64470 / % possible obs: 93.3 % / Redundancy: 2.3 % / Biso Wilson estimate: 41.14 Å2 / CC1/2: 0.993 / Rmerge(I) obs: 0.08 / Rpim(I) all: 0.06 / Rrim(I) all: 0.101 / Net I/σ(I): 6.8 / Num. measured all: 150043 / Scaling rejects: 1135
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
2.48-2.542.40.2721085244470.9010.2070.3432.591.4
11.37-102.82.50.03817046760.9970.0290.04914.194.2

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Processing

Software
NameVersionClassification
Aimless0.7.4data scaling
PHENIX1.12_2829refinement
PDB_EXTRACT3.27data extraction
autoXDSdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4TSM

4tsm


Resolution: 2.487→39.735 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 1.97 / Phase error: 24.15 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2182 3188 4.97 %
Rwork0.1864 60993 -
obs0.1879 64181 93.53 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 217.02 Å2 / Biso mean: 62.1066 Å2 / Biso min: 15.24 Å2
Refinement stepCycle: final / Resolution: 2.487→39.735 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15116 0 104 625 15845
Biso mean--72.23 52.2 -
Num. residues----1984
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00415673
X-RAY DIFFRACTIONf_angle_d0.77521263
X-RAY DIFFRACTIONf_chiral_restr0.0472341
X-RAY DIFFRACTIONf_plane_restr0.0052751
X-RAY DIFFRACTIONf_dihedral_angle_d10.8889417
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A5793X-RAY DIFFRACTION10.05TORSIONAL
12C5793X-RAY DIFFRACTION10.05TORSIONAL
13D5793X-RAY DIFFRACTION10.05TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.4874-2.52460.23551230.2216277596
2.5246-2.5640.29411270.2403274496
2.564-2.6060.26471530.2384268496
2.606-2.6510.30911740.2124268196
2.651-2.69910.2561390.2064270895
2.6991-2.75110.29271340.2121273696
2.7511-2.80720.25721330.2249272896
2.8072-2.86820.26231550.2173268395
2.8682-2.93490.30791540.2158267994
2.9349-3.00830.22641190.2113266294
3.0083-3.08960.2731380.2177265594
3.0896-3.18050.22731290.213265693
3.1805-3.28310.25141500.2146265193
3.2831-3.40040.22741270.206261092
3.4004-3.53640.2281290.1862256191
3.5364-3.69730.20211600.1742251690
3.6973-3.8920.2231400.1765259190
3.892-4.13560.18661150.1693252589
4.1356-4.45460.18371560.1528255291
4.4546-4.90210.18211400.1442262192
4.9021-5.60980.17781310.1702260092
5.6098-7.06130.20711250.1871266294
7.0613-39.7350.18251370.1707271395
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.18371.36670.65763.1972-0.79122.6824-0.53651.07550.5824-0.7580.40350.5256-0.433-0.27080.05380.56280.0222-0.07830.77670.08550.601323.0082-67.101428.125
24.30891.01661.27350.34070.26021.36470.1316-0.1355-0.07010.05980.0132-0.21650.1228-0.045-0.10440.39970.0747-0.01390.2797-0.05580.49244.9672-75.637240.0426
35.2796-2.6602-1.71771.82020.36012.73330.1422-0.98950.45490.61430.37650.01070.1190.2294-0.45780.54040.0130.04330.6399-0.24420.633749.2211-67.355352.2536
43.8672-0.0344-0.61940.85720.35071.0910.09120.7744-0.0446-0.1419-0.06940.0526-0.091-0.0884-0.06350.36210.1189-0.04160.4179-0.02750.458345.3315-73.731133.2516
56.3894-1.3051-0.78161.64170.16435.45970.21731.5602-1.7196-0.51890.6449-1.29220.70140.6045-0.72730.47920.00740.01590.7676-0.45081.001631.7807-83.841728.1398
61.74950.0381-0.05480.2281-0.02971.42570.1922-0.11670.13740.03330.2596-0.3558-0.34950.0783-0.42050.47160.05090.09110.4229-0.0880.7240.6744-59.693952.9102
71.55130.17650.15371.91610.54493.18240.5517-0.43040.08790.0888-0.2935-0.2973-0.7383-0.7254-0.21381.0480.07560.08041.0297-0.07880.860327.1544-47.862381.1279
81.7195-0.4692-0.03131.85730.16441.76010.7366-1.2551.41140.3299-0.23840.2696-1.2839-0.4122-0.13091.12860.20150.32451.1158-0.29071.151829.6504-38.503774.266
91.46070.4085-0.04690.27750.4633.3437-0.24440.29461.7237-0.03720.08370.1603-0.8950.49820.2441.27340.22060.47990.82540.03461.646133.4471-38.028864.0161
105.67850.25830.35053.2072-0.20792.6311-0.0088-0.9325-1.99580.38880.022-0.08150.489-0.0242-0.09310.3412-0.0037-0.09470.5290.15170.711649.8813-43.99936.9064
116.4095-0.46550.05160.9102-0.23090.1131-0.1486-0.24080.6930.00150.0188-0.1835-0.2091-0.0050.12060.35530.02360.00350.2677-0.01160.269637.125-28.922931.8523
123.4296-0.08240.69341.65730.65493.21090.1158-0.4618-0.12970.243-0.11730.06960.1488-0.13570.00350.2296-0.02160.01520.2820.01110.203117.1766-30.8438.6436
132.68550.1232-0.17730.87920.1770.9141-0.0412-0.0639-0.19650.0144-0.0174-0.0550.0508-0.02430.03290.1909-0.0117-0.00610.16810.01830.140835.8951-33.609931.4293
148.0564-0.90711.14870.95580.90282.11980.57011.7036-0.9381-0.4203-0.42760.30660.11-0.0235-0.10870.27860.0324-0.03620.4116-0.10180.344840.8726-36.628621.4883
151.09910.34370.41231.08640.66721.67720.2007-0.46680.00740.4541-0.2355-0.17840.0693-0.17270.02480.4589-0.0485-0.08110.60780.01270.340132.933-22.376254.078
162.5544-0.01071.5521.3652-0.0032.91830.27510.10191.03170.5934-0.2356-1.1847-0.61710.0166-0.15771.2263-0.19640.01780.7998-0.05371.2246.58521.535873.2638
173.2814-0.17281.58882.03860.27472.9593-0.1723-0.39180.82360.55030.2604-0.3365-0.49011.07130.09830.9498-0.1678-0.16291.15630.08690.722445.93-5.672375.7493
182.07420.58950.28853.72250.17913.29310.073-1.2148-0.20350.87510.09430.630.6984-0.7624-0.21221.0651-0.4046-0.22621.46340.15660.704336.875-16.236777.1742
196.44560.1586-0.17221.17480.96011.43930.0105-0.2345-0.70150.1294-0.07820.03730.3028-0.2330.08270.384-0.030.00730.3020.04370.327727.5989-63.5371105.739
203.89540.6516-0.11943.62141.51313.02480.17980.1105-0.03970.0001-0.04160.13320.17690.0271-0.14470.26290.00910.00780.17320.01510.282654.489-64.836297.9852
212.10730.41020.20760.2565-0.23690.57220.09830.120.0328-0.03060.0884-0.06830.10980.0231-0.19280.2948-0.0369-0.02580.2838-0.01690.402436.3309-66.471595.1806
220.91950.10640.43791.31640.3912.7135-0.08511.12590.0195-0.7642-0.04590.3847-0.4838-0.54840.24130.9353-0.102-0.1391.168-0.00580.713827.7801-87.254757.8656
233.7540.6561-0.33513.6479-0.55513.48470.33670.1858-1.66480.04570.1047-0.51680.66980.0899-0.48640.9185-0.0713-0.27780.8651-0.06811.071730.917-97.401770.1668
243.9427-0.2467-0.70242.8907-0.53562.77190.2495-0.16141.2929-0.08430.1253-0.372-0.73860.0065-0.32090.51260.02870.10460.3262-0.03760.626584.0284-13.1338104.7269
253.40470.78430.69113.38870.2482.4422-0.04991.60110.5139-0.7687-0.0028-0.2071-0.4184-0.2656-0.04410.5270.07590.05070.68010.14820.373778.9709-20.398493.101
264.72450.7912-0.5983.6745-1.06741.2406-0.08290.3629-0.703-0.11320.0351-0.04820.62830.11080.05880.35030.0132-0.030.2823-0.03620.314979.9128-33.9404102.7356
272.43-0.9360.89640.72810.5172-0.1269-0.04730.15870.0904-0.06260.01540.08320.0037-0.03250.02840.3964-0.00720.04220.29140.05240.234549.2242-29.3006104.7364
281.87580.0754-0.16521.3789-0.18432.49520.05890.24750.244-0.1056-0.04950.0149-0.29570.1164-0.01420.3705-0.0211-0.00070.3220.08560.295551.2929-26.7961100.7273
293.58970.8996-0.43480.75250.13130.59480.1015-0.34710.14220.1104-0.0093-0.10180.03610.0699-0.08330.2610.0310.01360.23710.03710.213373.3087-26.3027109.5789
300.7126-0.5478-0.3411.61450.46011.15630.27530.4159-0.008-0.5612-0.2681-0.2137-0.0515-0.26790.01250.52350.07680.07810.72210.01930.326765.543-38.019884.1897
315.7761-1.4954-2.43951.50481.02542.24710.15540.9238-1.281-0.1121-0.3486-0.29490.5079-0.17920.36681.03810.09940.20540.8326-0.03140.877776.7684-58.414266.1442
323.68490.5688-0.73312.2279-0.67472.67080.48681.9060.2953-1.3025-0.5043-0.2301-0.3406-0.436-0.00891.25330.3960.22821.39990.2610.710373.2707-45.174359.7817
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 72 )A1 - 72
2X-RAY DIFFRACTION2chain 'A' and (resid 73 through 169 )A73 - 169
3X-RAY DIFFRACTION3chain 'A' and (resid 170 through 200 )A170 - 200
4X-RAY DIFFRACTION4chain 'A' and (resid 201 through 282 )A201 - 282
5X-RAY DIFFRACTION5chain 'A' and (resid 283 through 314 )A283 - 314
6X-RAY DIFFRACTION6chain 'A' and (resid 315 through 1051 )A315 - 1051
7X-RAY DIFFRACTION7chain 'A' and (resid 1052 through 1096 )A0
8X-RAY DIFFRACTION8chain 'A' and (resid 1097 through 1121 )A0
9X-RAY DIFFRACTION9chain 'A' and (resid 1122 through 1152 )A0
10X-RAY DIFFRACTION10chain 'B' and (resid 1 through 72 )B1 - 72
11X-RAY DIFFRACTION11chain 'B' and (resid 73 through 128 )B73 - 128
12X-RAY DIFFRACTION12chain 'B' and (resid 129 through 234 )B129 - 234
13X-RAY DIFFRACTION13chain 'B' and (resid 235 through 282 )B235 - 282
14X-RAY DIFFRACTION14chain 'B' and (resid 283 through 314 )B283 - 314
15X-RAY DIFFRACTION15chain 'B' and (resid 315 through 1054 )B315 - 1054
16X-RAY DIFFRACTION16chain 'B' and (resid 1055 through 1085 )B0
17X-RAY DIFFRACTION17chain 'B' and (resid 1086 through 1110 )B0
18X-RAY DIFFRACTION18chain 'B' and (resid 1111 through 1151 )B0
19X-RAY DIFFRACTION19chain 'C' and (resid 1 through 128 )C1 - 128
20X-RAY DIFFRACTION20chain 'C' and (resid 129 through 234 )C129 - 234
21X-RAY DIFFRACTION21chain 'C' and (resid 235 through 1054 )C235 - 1054
22X-RAY DIFFRACTION22chain 'C' and (resid 1055 through 1096 )C0
23X-RAY DIFFRACTION23chain 'C' and (resid 1097 through 1152 )C0
24X-RAY DIFFRACTION24chain 'D' and (resid 0 through 42 )D0 - 42
25X-RAY DIFFRACTION25chain 'D' and (resid 43 through 72 )D43 - 72
26X-RAY DIFFRACTION26chain 'D' and (resid 73 through 105 )D73 - 105
27X-RAY DIFFRACTION27chain 'D' and (resid 106 through 169 )D106 - 169
28X-RAY DIFFRACTION28chain 'D' and (resid 170 through 245 )D170 - 245
29X-RAY DIFFRACTION29chain 'D' and (resid 246 through 314 )D246 - 314
30X-RAY DIFFRACTION30chain 'D' and (resid 315 through 1054 )D315 - 1054
31X-RAY DIFFRACTION31chain 'D' and (resid 1055 through 1101 )D0
32X-RAY DIFFRACTION32chain 'D' and (resid 1103 through 1152 )D0

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