詳細 | タイプ | Solution-ID | 内容 | 詳細 | Label | 溶媒系 |
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bicelle1 | 400 uM [U-100% 15N, U-100% 13C, U-85% 2H] DR5 ectodomain, 4.4 mM DGS-NTA (Ni), 44 mM DMPC, 88 mM D7PC, 95% H2O/5% D2O | (15N,13C, 2H)-labeled DR5-ECD anchored to DMPC-D7PC bicelle with q = 0.5 for triple resonance experiments | (15N, 13C, 2H)_sample95% H2O/5% D2Obicelle2 | 500 uM [U-100% 15N; U-100% 2H] DR5 ectodomain, 5.5 mM DGS-NTA (Ni), 55 mM DMPC, 110 mM D7PC, 95% H2O/5% D2O | (15N,2H)-labeled DR5-ECD anchored to DMPC-D7PC bicelle with q = 0.5 for measuring HN-HN NOEs | (15N,2H)_sample95% H2O/5% D2Obicelle3 | 250 uM [U-100% 15N; U-100% 2H] Isotopically mixed DR5 ectodomain, 250 uM [U-100% 13C] DR5 ectodomain, 5.5 mM DGS-NTA (Ni), 55 mM DMPC, 110 mM D7PC, 95% H2O/5% D2O | Isotopically mixed DR5-ECD anchored to DMPC-D7PC bicelle with q = 0.5 for measuring intermolecular NOEs using regular 15N-edited NOESY | mixed_NOE_sample195% H2O/5% D2Obicelle4 | 250 uM [U-100% 13C; U-100% 15N; U-95% 2H] Isotopically mixed DR5 ectodomain, 250 uM DR5 ectodomain, 5.5 mM DGS-NTA (Ni), 55 mM DMPC, 110 mM D7PC, 95% H2O/5% D2O | Isotopically mixed DR5-ECD anchored to DMPC-D7PC bicelle with q = 0.5 for measuring intermolecular NOEs using 13C-filtered 15N-edited NOESY | mixed_NOE_sample295% H2O/5% D2Obicelle5 | 500 uM [U-100% 13C; U-100% 15N] DR5 ectodomain, 5.5 mM DGS-NTA (Ni), 55 mM DMPC, 110 mM D7PC, 95% H2O/5% D2O | (15N,13C)-labeled DR5-ECD anchored to DMPC-D7PC bicelle with q = 0.5 for measuring both intra- and inter- molecular NOEs | (15N,13C)_sample95% H2O/5% D2Obicelle6 | 400 uM [U-100% 15N, U-85% 2H] DR5 ectodomain, 4.4 mM DGS-NTA (Ni), 44 mM DMPC, 88 mM D7PC, 95% H2O/5% D2O | (15N,2H)-labeled DR5-ECD anchored to DMPC-D7PC bicelle with q = 0.5 for performing paramagnetic probe titration analysis | PPT_sample95% H2O/5% D2O | | | | | | | | | | | | | | | | | |
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試料 | 濃度 (mg/ml) | 構成要素 | Isotopic labeling | Solution-ID |
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400 uMDR5 ectodomain[U-100% 15N, U-100% 13C, U-85% 2H] | 1 | 4.4 mMDGS-NTA (Ni) | natural abundance1 | 44 mMDMPCnatural abundance1 | 88 mMD7PCnatural abundance1 | 500 uMDR5 ectodomain[U-100% 15N; U-100% 2H] | 2 | 5.5 mMDGS-NTA (Ni) | natural abundance2 | 55 mMDMPCnatural abundance2 | 110 mMD7PCnatural abundance2 | 250 uMIsotopically mixed DR5 ectodomain[U-100% 15N; U-100% 2H] | 3 | 250 uMDR5 ectodomain[U-100% 13C] | 3 | 5.5 mMDGS-NTA (Ni) | natural abundance3 | 55 mMDMPCnatural abundance3 | 110 mMD7PCnatural abundance3 | 250 uMIsotopically mixed DR5 ectodomain[U-100% 13C; U-100% 15N; U-95% 2H] | 4 | 250 uMDR5 ectodomainnatural abundance4 | 5.5 mMDGS-NTA (Ni) | natural abundance4 | 55 mMDMPCnatural abundance4 | 110 mMD7PCnatural abundance4 | 500 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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