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- PDB-8db0: Crystal structure of DMATS1 prenyltransferase in complex with L-T... -

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Basic information

Entry
Database: PDB / ID: 8db0
TitleCrystal structure of DMATS1 prenyltransferase in complex with L-Trp and DMSPP
ComponentsDimethylallyltryptophan synthase 1
KeywordsTRANSFERASE / prenyltransferase / fungal protein / DMATS
Function / homology
Function and homology information


transferase activity, transferring alkyl or aryl (other than methyl) groups / Transferases; Transferring alkyl or aryl groups, other than methyl groups / alkaloid metabolic process
Similarity search - Function
Aromatic prenyltransferase DMATS-type, fungi / Aromatic prenyltransferase, DMATS-type / Tryptophan dimethylallyltransferase / Aromatic prenyltransferase
Similarity search - Domain/homology
DIMETHYLALLYL S-THIOLODIPHOSPHATE / 3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL / TRYPTOPHAN / Dimethylallyltryptophan synthase 1
Similarity search - Component
Biological speciesFusarium fujikuroi (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.26 Å
AuthorsEaton, S.A. / Ronnebaum, T.A. / Roose, B.W. / Christianson, D.W.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM56838 United States
CitationJournal: Biochemistry / Year: 2022
Title: Structural Basis of Substrate Promiscuity and Catalysis by the Reverse Prenyltransferase N -Dimethylallyl-l-tryptophan Synthase from Fusarium fujikuroi .
Authors: Eaton, S.A. / Ronnebaum, T.A. / Roose, B.W. / Christianson, D.W.
History
DepositionJun 14, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 28, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 5, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Apr 3, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Dimethylallyltryptophan synthase 1
B: Dimethylallyltryptophan synthase 1
C: Dimethylallyltryptophan synthase 1
D: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)191,13912
Polymers189,3654
Non-polymers1,7748
Water8,215456
1
A: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,9163
Polymers47,3411
Non-polymers5752
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,8083
Polymers47,3411
Non-polymers4662
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,8704
Polymers47,3411
Non-polymers5283
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,5462
Polymers47,3411
Non-polymers2041
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)90.500, 108.400, 182.040
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11SERSERPROPRO(chain 'A' and (resid 11 through 13 or (resid 14...AA11 - 15611 - 156
12GLYGLYSERSER(chain 'A' and (resid 11 through 13 or (resid 14...AA170 - 211170 - 211
13LEULEUTHRTHR(chain 'A' and (resid 11 through 13 or (resid 14...AA216 - 271216 - 271
14ARGARGPROPRO(chain 'A' and (resid 11 through 13 or (resid 14...AA276 - 307276 - 307
15HISHISASPASP(chain 'A' and (resid 11 through 13 or (resid 14...AA311 - 321311 - 321
16GLNGLNALAALA(chain 'A' and (resid 11 through 13 or (resid 14...AA327 - 411327 - 411
21SERSERPROPRO(chain 'B' and (resid 11 through 13 or (resid 14...BB11 - 15611 - 156
22GLYGLYSERSER(chain 'B' and (resid 11 through 13 or (resid 14...BB170 - 211170 - 211
23LEULEUTHRTHR(chain 'B' and (resid 11 through 13 or (resid 14...BB216 - 271216 - 271
24ARGARGPROPRO(chain 'B' and (resid 11 through 13 or (resid 14...BB276 - 307276 - 307
25HISHISASPASP(chain 'B' and (resid 11 through 13 or (resid 14...BB311 - 321311 - 321
26GLNGLNALAALA(chain 'B' and (resid 11 through 13 or (resid 14...BB327 - 411327 - 411
31SERSERPROPRO(chain 'C' and (resid 11 through 24 or (resid 25...CC11 - 15611 - 156
32GLYGLYSERSER(chain 'C' and (resid 11 through 24 or (resid 25...CC170 - 211170 - 211
33LEULEUTHRTHR(chain 'C' and (resid 11 through 24 or (resid 25...CC216 - 271216 - 271
34ARGARGPROPRO(chain 'C' and (resid 11 through 24 or (resid 25...CC276 - 307276 - 307
35HISHISASPASP(chain 'C' and (resid 11 through 24 or (resid 25...CC311 - 321311 - 321
36GLNGLNALAALA(chain 'C' and (resid 11 through 24 or (resid 25...CC327 - 411327 - 411
41SERSERPROPRO(chain 'D' and (resid 11 through 72 or (resid 73...DD11 - 15611 - 156
42GLYGLYSERSER(chain 'D' and (resid 11 through 72 or (resid 73...DD170 - 211170 - 211
43LEULEUTHRTHR(chain 'D' and (resid 11 through 72 or (resid 73...DD216 - 271216 - 271
44ARGARGPROPRO(chain 'D' and (resid 11 through 72 or (resid 73...DD276 - 307276 - 307
45HISHISASPASP(chain 'D' and (resid 11 through 72 or (resid 73...DD311 - 321311 - 321
46GLNGLNALAALA(chain 'D' and (resid 11 through 72 or (resid 73...DD327 - 411327 - 411

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
Dimethylallyltryptophan synthase 1 / DMATS 1 / DMATS1 biosynthesis cluster protein DMATS1


Mass: 47341.348 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Fusarium fujikuroi (fungus) / Gene: DMATS1 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: S0EH60, Transferases; Transferring alkyl or aryl groups, other than methyl groups

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Non-polymers , 5 types, 464 molecules

#2: Chemical
ChemComp-TRP / TRYPTOPHAN


Type: L-peptide linking / Mass: 204.225 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C11H12N2O2 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical ChemComp-PE8 / 3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL


Mass: 370.436 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C16H34O9
#4: Chemical ChemComp-DST / DIMETHYLALLYL S-THIOLODIPHOSPHATE / DMASPP / DMAPP / DMADP / Dimethylallyl pyrophosphate / dimethylallyl diphosphate / isoprenyl pyrophosphate


Mass: 262.158 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C5H12O6P2S / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2 / Feature type: SUBJECT OF INVESTIGATION
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 456 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.27 Å3/Da / Density % sol: 46 %
Crystal growTemperature: 294 K / Method: vapor diffusion, hanging drop / pH: 8.9
Details: 7.5 mg/mL protein, 0.1 M Tris-HCl (pH 8.9), 0.55 M NaCl, 25% (v/v) PEG 3,350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 9, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.26→93.14 Å / Num. obs: 84491 / % possible obs: 100 % / Redundancy: 7.8 % / Biso Wilson estimate: 39.58 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.21 / Rpim(I) all: 0.079 / Net I/σ(I): 7.4
Reflection shellResolution: 2.26→2.3 Å / Rmerge(I) obs: 2.19 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 4417 / CC1/2: 0.52 / Rpim(I) all: 1.26 / % possible all: 99.9

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Processing

Software
NameVersionClassification
iMOSFLMdata reduction
Aimlessdata scaling
PHASERphasing
PHENIX1.14_3260refinement
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: Alphafold

Resolution: 2.26→93.14 Å / SU ML: 0.2561 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 23.7039 / Stereochemistry target values: CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2324 2000 2.37 %
Rwork0.2062 157835 -
obs0.2068 84364 99.8 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 63.23 Å2
Refinement stepCycle: LAST / Resolution: 2.26→93.14 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11903 0 117 456 12476
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.004712385
X-RAY DIFFRACTIONf_angle_d0.77816959
X-RAY DIFFRACTIONf_chiral_restr0.05171852
X-RAY DIFFRACTIONf_plane_restr0.00542175
X-RAY DIFFRACTIONf_dihedral_angle_d18.87134246
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.26-2.290.27671380.29365721X-RAY DIFFRACTION99.22
2.29-2.320.33131430.28095933X-RAY DIFFRACTION99.79
2.32-2.350.31971410.28395807X-RAY DIFFRACTION99.6
2.35-2.380.32051410.28375822X-RAY DIFFRACTION99.9
2.38-2.420.27521420.26435871X-RAY DIFFRACTION99.87
2.42-2.460.28411420.26085844X-RAY DIFFRACTION99.88
2.46-2.50.29731470.25175854X-RAY DIFFRACTION99.97
2.5-2.540.26551400.25735836X-RAY DIFFRACTION99.83
2.54-2.590.29671430.25385874X-RAY DIFFRACTION99.85
2.59-2.640.27771440.24945844X-RAY DIFFRACTION99.83
2.64-2.690.30711390.25495807X-RAY DIFFRACTION99.75
2.69-2.750.28251450.25245858X-RAY DIFFRACTION99.65
2.75-2.810.28321440.24515862X-RAY DIFFRACTION99.78
2.81-2.880.28541440.24425822X-RAY DIFFRACTION99.47
2.88-2.960.22611400.23365790X-RAY DIFFRACTION99.81
2.96-3.050.26921430.2215901X-RAY DIFFRACTION99.98
3.05-3.150.24721410.22475835X-RAY DIFFRACTION99.93
3.15-3.260.25841440.21725868X-RAY DIFFRACTION99.97
3.26-3.390.26131460.21055861X-RAY DIFFRACTION99.92
3.39-3.540.20631360.19125855X-RAY DIFFRACTION99.9
3.54-3.730.20691420.18515865X-RAY DIFFRACTION99.93
3.73-3.960.20941410.18155847X-RAY DIFFRACTION99.95
3.96-4.270.17781380.16845853X-RAY DIFFRACTION99.65
4.27-4.70.18841320.15115872X-RAY DIFFRACTION99.88
4.7-5.380.18081450.16215825X-RAY DIFFRACTION99.92
5.38-6.780.24991370.20585882X-RAY DIFFRACTION99.95
6.78-93.140.18891340.18915826X-RAY DIFFRACTION99.62
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.26198924322-0.0846038567501-1.479603945072.4784538479-0.3506153774362.640296640150.0494933575859-0.08561514164560.07346604634920.1253897030160.03635827549570.363880734092-0.214182333054-0.0854516210011-0.1040632089060.2652225755620.03787014026660.02157321417240.2477372455690.08524127418320.23465042713318.1946614567-2.61441861497-16.0069229985
28.61240759864-0.4259483806443.629964290952.793937437252.504612774497.1344510138-0.1001594940890.257191388588-1.0505275578-0.05127379214040.04338497184031.167298594-0.0547082550964-0.5306274039050.1422533465050.2888170229390.002638841877870.04053920836040.4619253521450.006654235712150.7888491927283.94186826883-17.4786086085-18.6257460891
33.928495318230.998322206564-0.2593430599255.040410952140.3538956436932.58305901932-0.2428430470170.302715759472-1.19207999078-0.188090386553-0.00407666285150.6403584231770.446256630216-0.6191881402690.2560892472910.27082830749-0.03563246052820.06075156430180.386566013672-0.01241282816950.81588252911610.0052661467-20.659195348-19.7186418365
41.40999275083-0.891246042857-1.742662849556.734535223421.313048744851.88195972114-0.6516119563360.312343222816-2.09443350482-0.240936049740.1357228869260.5034913132320.723703077928-1.236632288250.4048829003270.525203512799-0.2232543578130.2869119226810.235404862578-0.04170184797511.4776709620413.9585645398-32.8973227965-22.6265685808
54.788663770190.230518010076-2.551667960292.50651720860.4765105179881.46004514447-0.3184637269190.408677799366-1.4537421712-0.281671543194-0.2162839693580.4281849320.441351158945-0.1004881582380.4838511774570.410363314760.01889665216980.07085595287810.317212616731-0.05930765433830.66287344582626.4533269619-29.3175554464-24.1608937433
62.523319027360.0139285214338-1.002678584854.148153209211.489836901914.37310763641-0.351283945258-0.134736609257-0.845570100820.0726014007602-0.06607951745680.03250268915330.492554315770.1732687459550.3873868585220.2921781163110.04620196415750.1399925416670.2908461800890.1651305570970.53583459803128.7203581052-27.8412190704-16.9618547236
73.53042131755-0.597145373934-0.6172927982223.408505576750.1350890840190.340088875251-0.0856101765834-0.0706540111055-0.05149078298020.09672132295020.00818739128217-0.114473580077-0.01892730980340.2280100895460.05094447186640.2315678350970.02629774785180.02205848095290.3480208503580.1170151113060.20008977003834.4724095209-13.4929725409-18.5912166323
83.48067790298-0.9711214927891.207597578312.703730561690.2567274015012.511217496290.05676993444780.09147173965290.0670946095929-0.178286907109-0.005600042642810.178294801758-0.0677902153281-0.099051797455-0.0461640270140.193319406847-0.03064032410590.01935068020710.193969057392-0.01533354731060.21932196375320.336603171239.2888313891-77.2394778521
94.5669825591-0.999438661990.435597322498.92647881301-1.484677719684.27682878458-0.0223520263744-0.1581308303540.402019890934-0.3178654745860.2296806432950.692465001223-0.514397610406-0.425369375764-0.1318204980770.234618813821-0.007628721356950.02333365258610.286145927084-0.04703308525410.37478579849413.523964167452.1638248937-77.0729006542
104.083680012380.01145747648491.141565506288.097666346111.073271392652.71814459716-0.247586701416-0.3188186162521.42502400940.434994878788-0.0005399637427910.619728862689-0.917919693988-0.4230411228690.2216449502920.5195229456230.120787606267-0.06997828812010.374092720688-0.1145539617150.7213032591813.373009518866.8467760325-72.4752125438
115.50132833673-0.4031595457481.085564553532.065844293010.5382966377960.456534969759-0.272725996301-0.4029770290411.033782816850.1259265509880.0433704003064-0.347674059624-0.4918627023870.03961885679680.1861533194190.524618672424-0.0110830447397-0.03687728173110.372609064283-0.1514054765660.64318827809428.086632704564.44611149-68.7385167095
129.04205961332-1.06974879974-1.405957390886.771995293572.527646001434.6610184210.07052294336290.3861622618471.16633834822-0.6201537510470.125812526933-0.210529827553-0.907097261590.16702159922-0.1583412204970.516457379463-0.0367680741119-0.08464576422470.258297321011-0.01812253108450.54062411211730.956448314262.9246234258-74.92082224
135.83254510463-0.03939224531550.5866829369545.47496326883-1.033864701832.49537878727-0.232267892720.2284106793430.103538244044-0.2948040002520.243578297099-0.169701648309-0.1148999539170.2803589302270.00985701150040.193959051662-0.0600397100814-0.006849794555660.254136001425-0.04637084096210.25852254356637.846857724449.6037426618-73.1060879779
142.33866420494-0.4105518651470.1403576676845.379699412970.9441165849892.934422701360.261884186122-0.0743899559183-0.1638489813660.185921383862-0.03257986390380.4709752997630.560603741911-0.347273101688-0.2260009258040.432965455606-0.0755695314465-0.07545590476340.3194676461120.1100192207280.32022662526218.65951083337.89230445154-69.4404466908
153.98444414984-0.450655972728-1.999892070517.75994368195-1.803390088031.734633529990.3361450809510.0238864469165-0.400722949289-0.0859250338773-0.0193409778566-0.1131459095621.29352386247-0.0197877650996-0.3486167819240.830276663183-0.00811241951817-0.2783989884370.4338567399140.08408797709090.42788080820122.6690969814-5.43690370632-73.9866247352
163.76312797957-1.344389931780.8554738808763.80641461006-2.528824477863.434289244750.695990209719-0.111403359356-1.76031553493-1.08320107878-0.451859252004-0.6299903128731.489722821570.662945664566-0.1813850958381.617639859490.28484723961-0.5017085418930.297347125132-0.04604164328031.1013026571331.467395683-19.2644141082-72.6093552402
170.367577583398-0.7984060069760.922383402742.82325354847-0.5849522300382.472266865970.358320399889-0.870256513917-1.237724352770.341874116249-0.0710406308882-0.03977999165231.04112320155-0.154709974399-0.3306085052741.08678693696-0.193364928027-0.4013836249490.89359265610.4752832329160.94483254174927.149155604-14.7998424302-56.1353161377
182.70521103933-1.61510840112-0.1529146804863.6569307572-0.9669528666874.064397041270.761106057752-0.385737887321-0.3334232240560.683210686791-0.3125819752-0.05373821239320.841691827902-0.134829490688-0.1785376949311.23730685244-0.325931695304-0.4994608369850.7066775489450.444593014620.55813220564225.196571063-10.8370339024-57.632504333
191.52387643564-1.335799252461.419675365873.95111521582-1.068611748343.927866209950.258563597866-1.7366185214-0.6154473055710.6143645565920.5041622916470.4519831490370.218514708613-0.564195320446-0.6608713241370.789190966056-0.166688299574-0.1142782364381.035212379360.279361406330.48441810999721.20042368731.33424227339-50.0458006242
202.18017432760.2016560512260.9033521336245.16325175783-1.026521739851.5168860550.369221385824-0.1520838851730.2963623737060.5170448651630.2097343964071.27994733132-0.179341043967-0.508691883884-0.6231668056070.7353470104850.04599179989230.240513455630.5526066617020.3239505541310.98345508058912.957362146525.4131520151-28.9181000479
212.782482334440.03706437722650.2956747879614.0081912012-0.1649651329121.250961117550.493227455499-0.4997809830171.077700582761.44708021523-0.09140550152610.201433487499-1.110714496680.313218359779-0.4194065227021.51755527652-0.08353681485180.4513739451710.559554073254-0.02128028844221.0552219566222.019675790443.58609748-20.6488778953
222.36706337930.71938870356-0.8081637032644.74200682248-1.962082588644.377105724050.1884103463230.6833971505891.04135795697-0.2175291408430.572533724621.19254551479-0.393742464995-0.0533687978505-0.6933804177650.7634740359920.05832973686710.212009999520.6570784488360.3589022663350.98131379055822.937377342341.6434925257-42.8723972825
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 11 through 137 )
2X-RAY DIFFRACTION2chain 'A' and (resid 138 through 172 )
3X-RAY DIFFRACTION3chain 'A' and (resid 173 through 211 )
4X-RAY DIFFRACTION4chain 'A' and (resid 212 through 243 )
5X-RAY DIFFRACTION5chain 'A' and (resid 244 through 294 )
6X-RAY DIFFRACTION6chain 'A' and (resid 295 through 343 )
7X-RAY DIFFRACTION7chain 'A' and (resid 344 through 414 )
8X-RAY DIFFRACTION8chain 'B' and (resid 11 through 153 )
9X-RAY DIFFRACTION9chain 'B' and (resid 154 through 199 )
10X-RAY DIFFRACTION10chain 'B' and (resid 200 through 243 )
11X-RAY DIFFRACTION11chain 'B' and (resid 244 through 294 )
12X-RAY DIFFRACTION12chain 'B' and (resid 295 through 343 )
13X-RAY DIFFRACTION13chain 'B' and (resid 344 through 412 )
14X-RAY DIFFRACTION14chain 'C' and (resid 11 through 153 )
15X-RAY DIFFRACTION15chain 'C' and (resid 154 through 199 )
16X-RAY DIFFRACTION16chain 'C' and (resid 200 through 227 )
17X-RAY DIFFRACTION17chain 'C' and (resid 228 through 315 )
18X-RAY DIFFRACTION18chain 'C' and (resid 316 through 343 )
19X-RAY DIFFRACTION19chain 'C' and (resid 344 through 412 )
20X-RAY DIFFRACTION20chain 'D' and (resid 11 through 124 )
21X-RAY DIFFRACTION21chain 'D' and (resid 125 through 262 )
22X-RAY DIFFRACTION22chain 'D' and (resid 263 through 412 )

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