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- PDB-8daz: Crystal structure of DMATS1 prenyltransferase in complex with L-T... -

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Basic information

Entry
Database: PDB / ID: 8daz
TitleCrystal structure of DMATS1 prenyltransferase in complex with L-Trp and GSPP
ComponentsDimethylallyltryptophan synthase 1
KeywordsTRANSFERASE / Prenyltransferase / fungal enzyme / DMATS
Function / homology
Function and homology information


alkaloid metabolic process / Transferases; Transferring alkyl or aryl groups, other than methyl groups / transferase activity, transferring alkyl or aryl (other than methyl) groups
Similarity search - Function
Aromatic prenyltransferase DMATS-type, fungi / Aromatic prenyltransferase, DMATS-type / Tryptophan dimethylallyltransferase / Aromatic prenyltransferase
Similarity search - Domain/homology
GERANYL S-THIOLODIPHOSPHATE / TRYPTOPHAN / Dimethylallyltryptophan synthase 1
Similarity search - Component
Biological speciesFusarium fujikuroi (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.49 Å
AuthorsEaton, S.A. / Ronnebaum, T.A. / Roose, B.W. / Christianson, D.W.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM56838 United States
CitationJournal: Biochemistry / Year: 2022
Title: Structural Basis of Substrate Promiscuity and Catalysis by the Reverse Prenyltransferase N -Dimethylallyl-l-tryptophan Synthase from Fusarium fujikuroi .
Authors: Eaton, S.A. / Ronnebaum, T.A. / Roose, B.W. / Christianson, D.W.
History
DepositionJun 14, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 28, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 5, 2022Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Apr 3, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Dimethylallyltryptophan synthase 1
B: Dimethylallyltryptophan synthase 1
C: Dimethylallyltryptophan synthase 1
D: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)190,1047
Polymers189,3654
Non-polymers7393
Water1,838102
1
A: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,5462
Polymers47,3411
Non-polymers2041
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Dimethylallyltryptophan synthase 1


Theoretical massNumber of molelcules
Total (without water)47,3411
Polymers47,3411
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Dimethylallyltryptophan synthase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)47,8763
Polymers47,3411
Non-polymers5352
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Dimethylallyltryptophan synthase 1


Theoretical massNumber of molelcules
Total (without water)47,3411
Polymers47,3411
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)89.832, 107.357, 179.301
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11SERSERPROPRO(chain 'A' and (resid 11 through 24 or (resid 25...AA11 - 7111 - 71
12SERSERLEULEU(chain 'A' and (resid 11 through 24 or (resid 25...AA87 - 15487 - 154
13SERSERALAALA(chain 'A' and (resid 11 through 24 or (resid 25...AA171 - 293171 - 293
14GLYGLYVALVAL(chain 'A' and (resid 11 through 24 or (resid 25...AA315 - 322315 - 322
15LYSLYSPHEPHE(chain 'A' and (resid 11 through 24 or (resid 25...AA325 - 409325 - 409
21SERSERPROPRO(chain 'B' and (resid 11 through 24 or (resid 25...BB11 - 7111 - 71
22SERSERLEULEU(chain 'B' and (resid 11 through 24 or (resid 25...BB87 - 15487 - 154
23SERSERALAALA(chain 'B' and (resid 11 through 24 or (resid 25...BB171 - 293171 - 293
24GLYGLYVALVAL(chain 'B' and (resid 11 through 24 or (resid 25...BB315 - 322315 - 322
25LYSLYSPHEPHE(chain 'B' and (resid 11 through 24 or (resid 25...BB325 - 409325 - 409
31SERSERPROPRO(chain 'C' and (resid 11 through 69 or (resid 70...CC11 - 7111 - 71
32SERSERLEULEU(chain 'C' and (resid 11 through 69 or (resid 70...CC87 - 15487 - 154
33SERSERALAALA(chain 'C' and (resid 11 through 69 or (resid 70...CC171 - 293171 - 293
34GLYGLYVALVAL(chain 'C' and (resid 11 through 69 or (resid 70...CC315 - 322315 - 322
35LYSLYSPHEPHE(chain 'C' and (resid 11 through 69 or (resid 70...CC325 - 409325 - 409
41SERSERPROPRO(chain 'D' and (resid 11 through 26 or (resid 27...DD11 - 7111 - 71
42SERSERLEULEU(chain 'D' and (resid 11 through 26 or (resid 27...DD87 - 15487 - 154
43SERSERALAALA(chain 'D' and (resid 11 through 26 or (resid 27...DD171 - 293171 - 293
44GLYGLYVALVAL(chain 'D' and (resid 11 through 26 or (resid 27...DD315 - 322315 - 322
45LYSLYSPHEPHE(chain 'D' and (resid 11 through 26 or (resid 27...DD325 - 409325 - 409

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Components

#1: Protein
Dimethylallyltryptophan synthase 1 / DMATS 1 / DMATS1 biosynthesis cluster protein DMATS1


Mass: 47341.348 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Fusarium fujikuroi (fungus) / Gene: DMATS1 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: S0EH60, Transferases; Transferring alkyl or aryl groups, other than methyl groups
#2: Chemical ChemComp-TRP / TRYPTOPHAN / Tryptophan


Type: L-peptide linking / Mass: 204.225 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C11H12N2O2 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical ChemComp-GST / GERANYL S-THIOLODIPHOSPHATE / S-[(2E)-3,7-DIMETHYLOCTA-2,6-DIENYL] TRIHYDROGEN THIODIPHOSPHATE


Mass: 330.275 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: C10H20O6P2S / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 102 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.27 Å3/Da / Density % sol: 46 %
Crystal growTemperature: 294 K / Method: vapor diffusion, hanging drop / pH: 8.9
Details: 7.5 mg/mL protein, 0.1 M Tris-HCl (pH 8.8), 0.6 M NaCl, 24% (v/v) PEG 3,350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Feb 16, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.49→92.11 Å / Num. obs: 51807 / % possible obs: 94.8 % / Redundancy: 13.4 % / Biso Wilson estimate: 50.65 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.109 / Rpim(I) all: 0.031 / Net I/σ(I): 13.2
Reflection shellResolution: 2.49→2.64 Å / Rmerge(I) obs: 1.42 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 2592 / CC1/2: 0.708 / Rpim(I) all: 0.396 / % possible all: 74.7

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Processing

Software
NameVersionClassification
STARANISOdata scaling
PHASERphasing
PHENIX1.14_3260refinement
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: Alphafold

Resolution: 2.49→92.11 Å / SU ML: 0.3121 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.7745 / Stereochemistry target values: CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2608 2000 3.86 %
Rwork0.2362 49793 -
obs0.2371 51793 94.8 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 58.46 Å2
Refinement stepCycle: LAST / Resolution: 2.49→92.11 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11522 0 49 102 11673
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.005411924
X-RAY DIFFRACTIONf_angle_d0.910116336
X-RAY DIFFRACTIONf_chiral_restr0.05371804
X-RAY DIFFRACTIONf_plane_restr0.00532086
X-RAY DIFFRACTIONf_dihedral_angle_d19.19724086
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.49-2.550.4031320.3698793X-RAY DIFFRACTION19.06
2.55-2.620.4103530.33741311X-RAY DIFFRACTION31.63
2.62-2.70.4043780.34431969X-RAY DIFFRACTION47.19
2.7-2.780.33951330.33413290X-RAY DIFFRACTION79.24
2.78-2.880.32291660.31524148X-RAY DIFFRACTION98.88
2.88-30.32381690.29764202X-RAY DIFFRACTION99.93
3-3.140.30521680.27814167X-RAY DIFFRACTION99.95
3.14-3.30.27121680.27074196X-RAY DIFFRACTION99.98
3.3-3.510.29781690.25074196X-RAY DIFFRACTION99.89
3.51-3.780.24821700.22044237X-RAY DIFFRACTION99.98
3.78-4.160.2291700.2134240X-RAY DIFFRACTION100
4.16-4.760.20891710.18124256X-RAY DIFFRACTION100
4.76-60.2481730.21244315X-RAY DIFFRACTION99.98
6-92.110.23811800.22414473X-RAY DIFFRACTION99.89
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.877554499930.218217744365-0.4799904259681.57948292727-0.5377736077181.040917599190.00573963235317-0.1197898011690.1103061412030.3926426675320.03376750691250.41697291855-0.128468143619-0.265348592024-0.06975643571070.2654072822970.1256732710770.2647176954380.3445719152310.1470049592970.18110968117620.3623.153-14.815
20.9295993781580.831564808166-1.069731532951.19091322661-1.963495799543.58069934946-0.403684970502-0.280356242367-0.6552172854140.08883711979950.07603393524130.4780222267570.913475177851-0.009911442801150.2541775327460.3024095943660.06615258089350.1983731925210.2783410663270.1187289896180.32970157254825.422-10.214-20.482
31.79234576398-0.259726466187-0.5037353897941.044799261370.2121437353531.25053770821-0.4085883890960.177471234609-1.06529473786-0.1614164044970.02798091878610.8177204294660.515837484764-0.3272820603880.2689215814970.416172597232-0.06841854665930.2465233775030.3479707620850.07275141234290.73492946860913.613-22.062-20.167
41.814449630710.300238510624-1.193566007951.89653990360.8204662952991.90760227894-0.505907054763-0.283650931563-0.9777248676990.03372946145280.07385942547340.02405708068970.5475065805920.2478411685520.4244294052950.5083612675280.09382161498170.2884956012250.3039826922930.1562558896530.61036079898928.687-27.121-16.96
52.564062180180.0830307758623-0.9793792240411.11032632997-0.7277605660481.20674650103-0.212032100057-0.203399292845-0.3136752969780.1859727540710.1458839242750.02724987558060.1170020151410.1752222008510.0954310924680.1688540703040.1043472834240.2209021047010.4292741199590.2326000162890.12298962525634.367-12.712-18.184
63.346413523850.9585705071560.3225789221393.94256937710.1728033050020.4571327638250.148091625655-0.10376395809-0.1759448098640.0268170993878-0.1512105810370.22939243250.408626433727-0.0594656531141-0.02810655216490.279823313658-0.0259145638912-0.03836285594320.143690160432-0.08168555663070.20736241607125.530.512-74.167
73.678518886970.5488134692722.171718583631.47200820806-1.076655438912.70938780633-0.1771463028540.5598922801130.254021759042-0.4670534621840.2611001844640.458389943331-0.09576750879810.109578700302-0.07323372990050.369016063837-0.0738504448185-0.07164772611750.336068576486-0.04481881011370.26057308877425.05341.556-79.028
80.680029374575-0.175999267437-0.5579699203370.156237305617-0.0150460033692.57181189719-0.134305015780.2811755043220.0959574922698-0.1591674145810.259480253837-0.0834334855435-0.699874226812-0.6141470044470.01740677058430.399756995205-0.0881863708899-0.06980091025740.0842320816359-0.07352712973840.48191455880718.70645.155-77.584
94.32866433354-0.5095807825140.05123187651787.1500906381-0.6982230986693.65666518173-0.174593898657-0.577340430135-0.09087450433130.3822201536090.2376131861950.972641662336-0.440660186651-0.663277321064-0.04559885529110.2440667596090.0601543017719-0.02496260671640.368964519424-0.07961937517110.391616765156.65545.637-73.093
102.44026933264-0.9820416640530.3915661349676.44490466594-2.351368126695.6424954079-0.2930593743050.1010601756040.4012700071230.2068123407250.285586918670.347932990327-0.761860959299-0.3448399032130.06882449488730.285994509902-0.0361670337929-0.1280243915340.208791060234-0.05259886535620.49158737796513.23352.306-76.615
111.10537631999-0.5407135103650.5939344481561.45910579132-0.4313932005630.81880485041-0.3474135593680.1112573233471.548222441230.24963626246-0.09821986640530.293999466457-1.3866599197-0.0658483248902-0.5304432266430.8890736430250.0882427174957-0.410179980931-0.0525287411642-0.151668230771.0351664476214.6764.53-72.091
121.41719101654-0.7777910361592.435093083276.41768750373-0.4085323816264.33179533031-0.7200423905490.02709288841481.0890102101-0.1516198118130.0691276917453-0.0124982210411-1.469947756180.3153151648560.5181072116750.766942770265-0.140291188061-0.2461205640260.4013206691850.03004214891950.84651743007435.09967.906-68.628
138.52827869982-0.914758889414-1.278135419422.242711660583.208691062414.917632788970.05646658664940.3851656079570.52563861032-0.435591143293-0.3439472541830.415131555531-0.980862287047-0.08182328327510.2981126178340.665863685726-0.0780999786256-0.1998463097370.2765870012680.09745447605910.647751788726.1260.696-71.637
146.80176253954-0.627320398260.7127555592035.99847151295-0.7529570180941.32803402393-0.5787772834810.4500977137760.0642827790603-0.3726879512260.561764971633-0.063876395295-0.1712775863070.4196778562870.002489420444870.238009823205-0.170489198588-0.0584081909070.393625887575-0.03274752236010.33149061159737.78149.976-72.075
150.764216399630.8751047978460.7111329525212.64191381947-0.5620049982631.94839008860.024147360502-0.1082169012090.3889630704070.03216900571780.2450009318870.792314683716-0.266089306145-0.60784193059-0.1670030336710.2876640493890.06655869560760.09331399584360.3648450855920.2204126655830.71580689081812.78425.527-28.61
162.77813568190.256972820338-0.3000950649364.65733527311-0.966595096482.124777462860.434693327713-0.3464837033230.4993906611011.23379861179-0.1344956092970.0433942659201-0.8835106928270.255693263868-0.2776884551760.710489318574-0.04264550998540.2153667056920.388239554246-0.06306007883660.50403348729621.7644.25-20.851
172.549486760320.770188715294-0.6519052310494.36837052056-0.5198495866932.915212143960.1332054122350.4174167002320.745052854786-0.43420672970.2796663600590.679249601173-0.132018145282-0.353979422333-0.367420258230.4258629600350.04855031546960.03427599527890.4309130652480.2115604510920.52503538331522.67742.317-42.225
181.57584058828-1.40813161487-1.210315544922.65372600527-0.9448035133273.88159916031-0.0418160252634-0.123458968783-0.124816261522-0.35377848980.1191461865620.8205568218970.0921639261879-0.163119935156-0.009264065160860.215367024139-0.00378039438102-0.1007007481380.3050294915050.1271416939960.59612740709417.25718.165-64.665
197.20572087596-2.12940099236.718238014961.01480845055-0.9985993833228.89936127105-0.236641688533-0.319916427267-0.358123816327-0.4169916251320.03970389479970.7098142815580.00387306070574-0.420890409120.1530943124710.361218177754-0.0573045625552-0.2319837748770.3301163620280.1222463984920.7295666870815.9836.296-63.391
202.12582942741-0.774421920457-1.706305298812.900196556091.606930286844.348373520130.2981915427970.597211591734-0.433551020081-0.478297765546-0.1609726394570.3587453512040.937706316906-0.375328814916-0.0808944192080.705587834205-0.0857821681063-0.2865119016490.3584952772750.0404848865110.41583170017223.371.823-73.908
214.758331806281.6575414524-2.236675681647.95228909911.276444767481.633364362890.216792667066-0.0919782233906-0.880440955615-0.365366353689-0.1393536031040.12456720581.12526588819-0.06984458938330.1300501832510.873203074623-0.142161929196-0.3369294938860.2406998749050.1096878809250.54583986320222.65-4.583-72.001
221.10641217963-0.1193717726950.02157532052372.396211370110.6661738568860.9684502563330.268436092352-0.253568448032-0.449953700274-0.386148955201-0.000618911004360.3729659800581.405779944420.177204828982-0.1461944954381.213121060940.036266835256-0.3534275455090.284980395591-0.003530220998230.51293901084329.458-15.101-67.085
233.066721112772.54785699057-2.276312127072.63332409722-0.8513847312625.189626920190.31667245152-0.450413000511-0.904935248241-0.644816087877-0.1104625411680.05471240338940.3714337145540.583997340111-0.028906748360.865219926680.0717849974052-0.2000122416760.3671433709970.1621018384870.4935107559632.043-13.246-48.152
241.82897783911-2.172991737052.305692745056.264059007270.4719757460767.134159391070.7356058394530.210872065718-0.452742273235-0.481423813571-0.3542531750950.7099553381521.31263224729-0.134073923439-0.2562665868010.790905165192-0.0400897454783-0.1711394222870.2300297424290.09967340057620.29897849921529.441-11.105-59.103
255.29813642685-0.3131434904-0.97072937581.429932946142.958782648987.969810481830.646301085255-1.39146472233-0.7306712236371.37680927186-0.1458457246540.5866492531530.404200261725-0.45641422558-0.3475753773140.9309014521630.1297924260650.233487356820.6807356914810.4079859025740.61452517738321.391-0.695-42.98
263.77403373811-0.773684146623-0.6361722122676.170445253941.096622104235.42079881420.366456037821-0.542661597147-0.2289526988450.4559835594540.3106862260641.01474731428-0.468000631228-0.688205539973-0.6299316184570.5290538450720.02478815882680.1109587090370.6709884281390.2740737535190.44141404036620.4474.078-48.372
276.083318041740.5634707579263.553650824085.95118148435-0.5494065663836.614424211690.25933487787-0.31024796254-0.718706139075-0.3236499281540.3200085195260.7879234838970.0632567606088-0.380288117488-0.5341914211640.398961294509-0.149909109961-0.08873207689840.2419606640760.1973447132080.49605265005621.9970.131-54.384
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 11:81 )A11 - 81
2X-RAY DIFFRACTION2( CHAIN A AND RESID 82:108 )A82 - 108
3X-RAY DIFFRACTION3( CHAIN A AND RESID 109:293 )A109 - 293
4X-RAY DIFFRACTION4( CHAIN A AND RESID 294:343 )A294 - 343
5X-RAY DIFFRACTION5( CHAIN A AND RESID 344:414 )A344 - 414
6X-RAY DIFFRACTION6( CHAIN B AND RESID 11:62 )B11 - 62
7X-RAY DIFFRACTION7( CHAIN B AND RESID 63:95 )B63 - 95
8X-RAY DIFFRACTION8( CHAIN B AND RESID 96:124 )B96 - 124
9X-RAY DIFFRACTION9( CHAIN B AND RESID 125:154 )B125 - 154
10X-RAY DIFFRACTION10( CHAIN B AND RESID 155:199 )B155 - 199
11X-RAY DIFFRACTION11( CHAIN B AND RESID 200:262 )B200 - 262
12X-RAY DIFFRACTION12( CHAIN B AND RESID 263:316 )B263 - 316
13X-RAY DIFFRACTION13( CHAIN B AND RESID 317:343 )B317 - 343
14X-RAY DIFFRACTION14( CHAIN B AND RESID 344:409 )B344 - 409
15X-RAY DIFFRACTION15( CHAIN C AND RESID 11:124 )C11 - 124
16X-RAY DIFFRACTION16( CHAIN C AND RESID 125:262 )C125 - 262
17X-RAY DIFFRACTION17( CHAIN C AND RESID 263:413 )C263 - 413
18X-RAY DIFFRACTION18( CHAIN D AND RESID 11:62 )D11 - 62
19X-RAY DIFFRACTION19( CHAIN D AND RESID 63:95 )D63 - 95
20X-RAY DIFFRACTION20( CHAIN D AND RESID 96:153 )D96 - 153
21X-RAY DIFFRACTION21( CHAIN D AND RESID 154:199 )D154 - 199
22X-RAY DIFFRACTION22( CHAIN D AND RESID 200:262 )D200 - 262
23X-RAY DIFFRACTION23( CHAIN D AND RESID 263:293 )D263 - 293
24X-RAY DIFFRACTION24( CHAIN D AND RESID 294:336 )D294 - 336
25X-RAY DIFFRACTION25( CHAIN D AND RESID 337:357 )D337 - 357
26X-RAY DIFFRACTION26( CHAIN D AND RESID 358:395 )D358 - 395
27X-RAY DIFFRACTION27( CHAIN D AND RESID 396:410 )D396 - 410

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